FastQCFastQC Report
Thu 26 May 2016
SRR937885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195569
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56982.9135496934585747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47152.41091379513113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36771.8801548302645101No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23641.2087805327020131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.6355813037853647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.5266683370063763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.4903640147467135No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.448946407661746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.35281665294601905No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.25770955519535305No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.14521728903865133No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.11913953642959774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2190.11198093767417128No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.10175436802356201No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.049.8822861
GTATCAA30300.038.580141
TGTATCG500.001613348337.9961139
GTATAGA550.002569251234.5595931
CCGGGTT300.003949770231.6634360-61
TAAGGTG804.4828493E-429.6844645
AGTATGA1652.6211637E-928.7849336
TATCAAC41050.028.4623762
TAGTATG1703.605237E-927.9383185
ATCAACG42400.027.5561433
GACAGGT350.00838910427.14008156-57
ACTATCC700.00837902727.1400818
AGCCGCC350.00838910427.14008116-17
GGACGGG350.00838910427.14008146-47
CGTAAGT350.00838910427.14008162-63
TCAACGC43200.027.0458434
CAACGCA43950.026.6923775
ATGGGAT6350.026.1784255
AACGCAG44650.026.1675366
ATGGGAG5000.025.6473775
Produced by FastQC (version 0.11.5)