##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 195569 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03726561980682 31.0 31.0 34.0 30.0 34.0 2 32.18468162132035 33.0 31.0 34.0 30.0 34.0 3 32.1729312927918 34.0 31.0 34.0 30.0 34.0 4 35.904422480045405 37.0 35.0 37.0 35.0 37.0 5 35.84094104893925 37.0 35.0 37.0 35.0 37.0 6 35.93260690600248 37.0 35.0 37.0 35.0 37.0 7 35.877710680118014 37.0 35.0 37.0 35.0 37.0 8 35.8770612929452 37.0 35.0 37.0 35.0 37.0 9 37.46240457332195 39.0 37.0 39.0 34.0 39.0 10-11 37.40922385449637 39.0 37.0 39.0 34.0 39.0 12-13 37.284894333969085 39.0 37.0 39.0 34.0 39.0 14-15 38.19745205017155 40.0 37.5 41.0 33.0 41.0 16-17 38.20969836732816 40.0 37.5 41.0 33.5 41.0 18-19 38.410264407958316 40.0 38.0 41.0 34.0 41.0 20-21 38.44270820017488 40.0 38.0 41.0 34.0 41.0 22-23 38.33656919041361 40.0 38.0 41.0 34.0 41.0 24-25 38.23371546615262 40.0 37.0 41.0 34.0 41.0 26-27 37.95145447386856 40.0 37.0 41.0 33.0 41.0 28-29 37.804102388415345 40.0 36.5 41.0 33.0 41.0 30-31 37.456508955918366 39.0 35.5 41.0 33.0 41.0 32-33 36.9818350556581 39.0 35.0 41.0 33.0 41.0 34-35 36.65559470059161 38.5 35.0 41.0 32.0 41.0 36-37 36.32451717808037 38.0 35.0 41.0 31.0 41.0 38-39 36.058319570075014 38.0 35.0 41.0 31.0 41.0 40-41 35.71009720354453 38.0 35.0 40.0 30.0 41.0 42-43 35.2294688831052 38.0 35.0 40.0 28.0 41.0 44-45 34.51609406398765 38.0 34.5 40.0 24.0 41.0 46-47 33.62068630508925 38.0 33.5 40.0 16.0 41.0 48-49 32.818611334107146 38.0 33.0 40.0 2.0 41.0 50-51 32.39624889425215 37.0 33.0 40.0 2.0 41.0 52-53 32.06672581032781 37.0 33.0 40.0 2.0 41.0 54-55 31.781069596919757 37.0 32.0 40.0 2.0 41.0 56-57 31.478636695999874 36.0 31.5 40.0 2.0 41.0 58-59 31.19481103855928 36.0 31.0 40.0 2.0 41.0 60-61 30.836505274353296 35.5 31.0 39.5 2.0 41.0 62-63 30.390570591453653 35.0 30.0 39.0 2.0 41.0 64-65 29.984966942613603 35.0 29.0 39.0 2.0 40.0 66-67 29.536122800648364 35.0 28.5 38.5 2.0 40.0 68-69 29.011752885170964 35.0 28.0 38.0 2.0 40.0 70-71 28.57478179057008 35.0 26.0 37.0 2.0 40.0 72-73 28.06544237583666 34.0 26.0 37.0 2.0 39.0 74-75 27.588730831573514 34.0 25.5 36.0 2.0 39.0 76-77 26.36034852149369 32.5 23.5 35.0 2.0 37.5 78-79 26.861212666629168 33.5 24.0 35.0 2.0 37.5 80-81 26.640045201437857 34.0 24.0 35.0 2.0 37.0 82-83 26.227541174726056 33.0 21.0 35.0 2.0 37.0 84-85 25.779806615567907 33.0 19.5 35.0 2.0 36.0 86-87 25.448199868077253 33.0 18.0 35.0 2.0 36.0 88-89 25.021864405913004 33.0 9.5 35.0 2.0 35.5 90-91 24.43930019583881 32.5 2.0 35.0 2.0 35.0 92-93 23.937052907158087 32.0 2.0 35.0 2.0 35.0 94-95 23.4183843042609 32.0 2.0 34.0 2.0 35.0 96-97 22.901221563744766 31.0 2.0 34.0 2.0 35.0 98-99 22.143762559505852 31.0 2.0 34.0 2.0 35.0 100-101 20.13379165409651 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3111993878558046 1101 2 0.29869686789142236 1101 3 0.3423492094302816 1101 4 0.26658123543712975 1101 5 0.1463976063378638 1101 6 0.17017697084612138 1101 7 0.28687312836672163 1101 8 0.2936883004920645 1101 9 0.15921534257021364 1101 10-11 0.2604336684015962 1101 12-13 0.24795998645913642 1101 14-15 0.4794505694846407 1101 16-17 0.5327058110073253 1101 18-19 0.547793173213222 1101 20-21 0.6135828985878717 1101 22-23 0.4695025510533313 1101 24-25 0.2644823947837551 1101 26-27 0.411246907630634 1101 28-29 0.5718967936001178 1101 30-31 0.7895739927899825 1101 32-33 1.0500453154109763 1101 34-35 0.9874020880725922 1101 36-37 1.1409464657379615 1101 38-39 1.2913197770312834 1101 40-41 1.4997504189427104 1101 42-43 1.5806079402640236 1101 44-45 1.7512082887999725 1101 46-47 2.2444571666413395 1101 48-49 2.3069017322351186 1101 50-51 2.51584661190536 1101 52-53 2.296075392926756 1101 54-55 2.723140540832752 1101 56-57 2.65301598972599 1101 58-59 2.7291377492363544 1101 60-61 2.692053751666027 1101 62-63 2.858847702088113 1101 64-65 2.9219854978086204 1101 66-67 2.9682422917015465 1101 68-69 3.309241323344306 1101 70-71 3.147369526517867 1101 72-73 3.4537295532446954 1101 74-75 3.1348362249010258 1101 76-77 3.337733867827044 1101 78-79 3.4369662049963594 1101 80-81 3.6141118813358375 1101 82-83 3.5704111570274293 1101 84-85 3.5869394273925153 1101 86-87 3.5929678940598784 1101 88-89 3.479164801615891 1101 90-91 3.4681005438485712 1101 92-93 3.4636295333805016 1101 94-95 3.5620860935943632 1101 96-97 3.770997386196509 1101 98-99 3.611353859815793 1101 100-101 3.497120599175254 1102 1 0.24409863966856093 1102 2 0.23856381018760686 1102 3 0.42851141810421467 1102 4 0.16243315102734357 1102 5 0.19884941489427632 1102 6 0.16613172283202005 1102 7 0.10054512223850764 1102 8 0.11953276569033733 1102 9 0.13528489355292805 1102 10-11 0.27364499719472946 1102 12-13 0.17591941855037874 1102 14-15 0.5436367453348723 1102 16-17 0.38049482850000516 1102 18-19 0.33992199153908587 1102 20-21 0.20386118646682405 1102 22-23 0.18720615137653596 1102 24-25 0.3586118007461607 1102 26-27 0.32622062138867136 1102 28-29 0.3669952751892538 1102 30-31 0.6146157044912997 1102 32-33 0.6844514352258741 1102 34-35 0.6059787484081838 1102 36-37 0.8759827208141928 1102 38-39 0.6072924729188784 1102 40-41 0.860485804106986 1102 42-43 0.825707471204133 1102 44-45 0.8929729823370209 1102 46-47 1.002280078170564 1102 48-49 1.361591281748744 1102 50-51 1.412787229133599 1102 52-53 1.0204659823471935 1102 54-55 1.455220249532914 1102 56-57 1.4303568294166418 1102 58-59 1.6300422897780287 1102 60-61 1.417614367425184 1102 62-63 1.3268083934005332 1102 64-65 1.5255485690490609 1102 66-67 1.445925219810512 1102 68-69 1.217897944224294 1102 70-71 1.1930923719379756 1102 72-73 1.5971495979470482 1102 74-75 1.856472268008364 1102 76-77 1.4669535704409746 1102 78-79 1.4491439230777239 1102 80-81 1.4424485551031125 1102 82-83 1.6287445778066783 1102 84-85 2.0352588918037746 1102 86-87 1.772438391557177 1102 88-89 1.9492714156750957 1102 90-91 1.935171910208478 1102 92-93 1.8179061632849063 1102 94-95 2.006090102115092 1102 96-97 1.978191736490853 1102 98-99 2.30173498409043 1102 100-101 2.114671518931381 1103 1 0.32420518801761133 1103 2 0.37683450861047874 1103 3 0.404272449842189 1103 4 0.15418424658496122 1103 5 0.28211179410953235 1103 6 0.22324913692546033 1103 7 0.2427137404689006 1103 8 0.1876330961152064 1103 9 0.2617602835401982 1103 10-11 0.4946820469615645 1103 12-13 0.5083947929147286 1103 14-15 0.522221620781103 1103 16-17 0.22432025793515464 1103 18-19 0.32499428662489294 1103 20-21 0.47739907596587017 1103 22-23 0.46985220619738044 1103 24-25 0.40403936114829975 1103 26-27 0.35566202370242905 1103 28-29 0.28754488969357794 1103 30-31 0.7154265343029635 1103 32-33 1.1066414568636276 1103 34-35 1.047666180546095 1103 36-37 1.174681331155007 1103 38-39 1.374870216499808 1103 40-41 1.3579275501250976 1103 42-43 1.3696664766487245 1103 44-45 1.2195233623646686 1103 46-47 1.8435706880114395 1103 48-49 1.8733670615279934 1103 50-51 2.008075354929396 1103 52-53 1.993832800816623 1103 54-55 2.0647330486142863 1103 56-57 2.3236991198936856 1103 58-59 2.0568606029708043 1103 60-61 2.0851038414794125 1103 62-63 1.7920395736344048 1103 64-65 1.8584613853156497 1103 66-67 2.264019691638474 1103 68-69 2.592773937256471 1103 70-71 2.392069851491474 1103 72-73 2.270544537681616 1103 74-75 2.407344417800971 1103 76-77 2.3208167292401995 1103 78-79 2.819598628312189 1103 80-81 3.031175052365196 1103 82-83 3.0478412602785383 1103 84-85 2.9885697802014093 1103 86-87 3.1962258572843822 1103 88-89 3.0422981230655175 1103 90-91 3.040177495404194 1103 92-93 3.3024316559929083 1103 94-95 3.6707088636858245 1103 96-97 3.900452061291464 1103 98-99 4.186965070719548 1103 100-101 4.4787911280457955 1104 1 0.20069439407296485 1104 2 0.2128800133729456 1104 3 0.22901641090635394 1104 4 0.217365399730582 1104 5 0.1011527304805 1104 6 0.21666527857545503 1104 7 0.2437140091978165 1104 8 0.3128010516853621 1104 9 0.5263686857975145 1104 10-11 0.33972250559224193 1104 12-13 0.2702114003615037 1104 14-15 0.38210188712131554 1104 16-17 0.3278704654535929 1104 18-19 0.4706602864457423 1104 20-21 0.3848816832334947 1104 22-23 0.32782151138384563 1104 24-25 0.539792249984103 1104 26-27 0.39017875954191084 1104 28-29 0.28092370788354515 1104 30-31 0.29649308949689157 1104 32-33 0.5819333263210922 1104 34-35 0.31138628445461336 1104 36-37 0.14153809864478006 1104 38-39 0.3691746564986218 1104 40-41 0.29264881852556357 1104 42-43 0.5907258657383281 1104 44-45 0.5109150795424213 1104 46-47 0.9480390526467204 1104 48-49 1.368612052719655 1104 50-51 1.1569661016971793 1104 52-53 1.5297603634453942 1104 54-55 1.4792473957523242 1104 56-57 0.9835181757594462 1104 58-59 1.3303370593322192 1104 60-61 1.4599732093692914 1104 62-63 1.4704457125971118 1104 64-65 1.853616800203234 1104 66-67 1.9217038455902795 1104 68-69 2.000659637892465 1104 70-71 2.339660248911674 1104 72-73 1.9228242546204974 1104 74-75 1.855084109061309 1104 76-77 2.089448600489188 1104 78-79 1.9486183649578557 1104 80-81 2.0000771454648323 1104 82-83 1.8575737257170104 1104 84-85 2.3546382762152653 1104 86-87 2.513378226577629 1104 88-89 2.3545909778623297 1104 90-91 2.54058058877256 1104 92-93 2.6137839595968586 1104 94-95 2.7542163731659812 1104 96-97 2.6280729146550534 1104 98-99 2.613870806588885 1104 100-101 2.630809580764435 1105 1 0.18597156729022757 1105 2 0.19028367487595688 1105 3 0.24284306562405789 1105 4 0.27836911244342133 1105 5 0.11958827010411 1105 6 0.24246223159043012 1105 7 -0.02622581924924816 1105 8 0.0593123818607495 1105 9 0.07437790356733132 1105 10-11 0.15235853170925395 1105 12-13 0.11053274205910668 1105 14-15 0.1811673076820668 1105 16-17 -0.13785095305876638 1105 18-19 -0.28820541576908454 1105 20-21 -0.2631147320782432 1105 22-23 0.20623016584550413 1105 24-25 0.27321306678092583 1105 26-27 -0.2730525808754507 1105 28-29 -0.2807841400232576 1105 30-31 -0.08143547278706365 1105 32-33 0.06471402021432482 1105 34-35 0.08020589782038456 1105 36-37 0.016042003394488802 1105 38-39 -0.05871549841112511 1105 40-41 0.22620406113213676 1105 42-43 0.4716950118143828 1105 44-45 0.3825064250807628 1105 46-47 0.5760316272210417 1105 48-49 0.7643360317236301 1105 50-51 0.4009809525485437 1105 52-53 0.08653926499448517 1105 54-55 0.46852533711707167 1105 56-57 0.610582572123544 1105 58-59 0.5009938254313084 1105 60-61 0.7085009266910163 1105 62-63 0.5070927488280716 1105 64-65 0.6722059693237057 1105 66-67 0.8119867799136671 1105 68-69 0.929221921850985 1105 70-71 0.576377698662025 1105 72-73 1.4869325634157846 1105 74-75 1.6301769268823314 1105 76-77 1.6095164535169708 1105 78-79 1.354392273548303 1105 80-81 1.6164194831936776 1105 82-83 1.0858735592850586 1105 84-85 1.0880270868669086 1105 86-87 0.9506461820250749 1105 88-89 0.15010371630811292 1105 90-91 0.10001600037158553 1105 92-93 0.44184694769057486 1105 94-95 0.685403161029253 1105 96-97 1.1163586655192184 1105 98-99 0.836249503298653 1105 100-101 0.7159459269429043 1106 1 -0.4203558952016202 1106 2 -0.43398246993813316 1106 3 -0.7161697632517843 1106 4 -0.23130690408190446 1106 5 -0.2516880943229438 1106 6 -0.41692024286053453 1106 7 -0.30346000059302014 1106 8 -0.31381221044192387 1106 9 -0.6962244012815475 1106 10-11 -0.48348621714919204 1106 12-13 -1.364190023759548 1106 14-15 -0.961689835175072 1106 16-17 -1.085013379673235 1106 18-19 -0.6644828673890046 1106 20-21 -0.4957740058334039 1106 22-23 -0.773276248592417 1106 24-25 -0.4038362917752778 1106 26-27 -0.19586188724640152 1106 28-29 -0.37743558598977245 1106 30-31 -0.6131271448896882 1106 32-33 -1.0167167255999843 1106 34-35 -1.8651083009184788 1106 36-37 -1.5242384923654555 1106 38-39 -1.0605093666433518 1106 40-41 -1.1279490156236776 1106 42-43 -1.2355239488292398 1106 44-45 -1.2967173192114672 1106 46-47 -1.8689020540140149 1106 48-49 -2.0788363970652384 1106 50-51 -1.6178818437115083 1106 52-53 -1.779355494735892 1106 54-55 -1.1808549629481604 1106 56-57 -1.4669342793659084 1106 58-59 -1.721880712511723 1106 60-61 -2.3886397560661052 1106 62-63 -1.9561601322330304 1106 64-65 -2.1588442589867967 1106 66-67 -2.303744900886503 1106 68-69 -1.9822805786926132 1106 70-71 -2.6786233885308413 1106 72-73 -2.1351048360188756 1106 74-75 -2.3180726926001647 1106 76-77 -1.9752302305447813 1106 78-79 -2.3268971371931606 1106 80-81 -1.5549221128054533 1106 82-83 -0.9690840401930885 1106 84-85 -0.9737254680363314 1106 86-87 -1.3612694518083686 1106 88-89 -2.1114748877362466 1106 90-91 -1.518053145094818 1106 92-93 -1.7868984317397398 1106 94-95 -1.6010721596926452 1106 96-97 -1.8000905625738461 1106 98-99 -2.0078470394280252 1106 100-101 -2.1878375042377876 1107 1 0.15960816589451454 1107 2 0.045801896638685946 1107 3 -0.11254592377888528 1107 4 0.09498839391150682 1107 5 0.04825200750395453 1107 6 0.13713785836737458 1107 7 -0.053235382443872936 1107 8 0.030752030348736525 1107 9 0.4404931788322557 1107 10-11 -0.3498384184070531 1107 12-13 0.05270126105627071 1107 14-15 0.13871447749338728 1107 16-17 0.025498749706812873 1107 18-19 0.13800290679488114 1107 20-21 0.018676428428349823 1107 22-23 -0.01863414017672227 1107 24-25 0.45135987474164097 1107 26-27 0.2925328417143689 1107 28-29 -0.3926347474153431 1107 30-31 -0.4256458630687874 1107 32-33 -0.11472511134422092 1107 34-35 -0.030352385973884566 1107 36-37 0.12311101347461317 1107 38-39 0.1964537959801902 1107 40-41 0.20029297299566906 1107 42-43 -0.09641044179120684 1107 44-45 0.8951065801595988 1107 46-47 1.3578874039075188 1107 48-49 2.2409449746029253 1107 50-51 1.79953355011898 1107 52-53 1.7308271946920861 1107 54-55 1.646413680358222 1107 56-57 2.199882132733517 1107 58-59 2.3768015276799552 1107 60-61 1.774732511079744 1107 62-63 1.3538877863054566 1107 64-65 0.7625393888119447 1107 66-67 0.909492595369862 1107 68-69 -0.0018972303847384353 1107 70-71 -0.5204173388153563 1107 72-73 -0.2701752163722766 1107 74-75 0.23905518999941577 1107 76-77 0.025967732917333564 1107 78-79 0.2913268819742534 1107 80-81 0.1619092738372565 1107 82-83 1.1674837944879535 1107 84-85 0.9412191261535447 1107 86-87 1.4968467506474568 1107 88-89 1.14144514820266 1107 90-91 1.0410214281307013 1107 92-93 1.473508875837382 1107 94-95 1.0043486249734208 1107 96-97 1.1168755949583513 1107 98-99 0.7540577224767375 1107 100-101 0.8692155056454105 1108 1 -0.16746638988493245 1108 2 -0.12538330211566517 1108 3 -0.181880966827066 1108 4 -0.07280790577708274 1108 5 -0.24768131361712875 1108 6 -0.7369233249995375 1108 7 -0.3136310593077667 1108 8 -0.35310948274890563 1108 9 0.26024038528032634 1108 10-11 0.05696685728445772 1108 12-13 -0.6251701439629755 1108 14-15 -0.41349288649269056 1108 16-17 -0.33431989839723286 1108 18-19 -0.22396815017525284 1108 20-21 -0.297361307420708 1108 22-23 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2316 94-95 2.1808451635915844 2316 96-97 2.6081542701314184 2316 98-99 2.689309521037888 2316 100-101 3.422731183669029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 12.0 6 43.0 7 82.0 8 144.0 9 255.0 10 418.0 11 752.0 12 1118.0 13 1559.0 14 2017.0 15 2944.0 16 4224.0 17 5988.0 18 4998.0 19 2773.0 20 2123.0 21 2010.0 22 2078.0 23 2245.0 24 2524.0 25 2657.0 26 2962.0 27 3259.0 28 3892.0 29 4315.0 30 5012.0 31 6203.0 32 7364.0 33 9103.0 34 12691.0 35 18665.0 36 21992.0 37 31054.0 38 25692.0 39 2393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.41158115504629 20.946851501355567 16.620799017852576 16.020768325745564 2 15.661991419908064 24.01607616749076 40.541701394392774 19.780231018208408 3 18.69877127765648 31.682424106069977 31.903829339005668 17.714975277267868 4 10.963393993935643 20.094698034964644 37.905291738465706 31.036616232634007 5 10.401006351056976 41.47414066415079 32.34641385164503 15.778439133147199 6 25.21002817419939 40.341260629240836 17.563622046438855 16.88508915012093 7 23.184656054896227 40.2164964795034 20.27161768992018 16.327229775680195 8 27.429705116864124 33.66433330435805 19.075620369281427 19.830341209496392 9 28.67427864334327 16.654480004499693 21.1229796133334 33.54826173882364 10-11 23.910742500089484 30.660278469491587 24.38039771129371 21.04858131912522 12-13 26.96656217880209 29.811001396012415 27.055027434456452 16.167408990729047 14-15 23.19411563182304 28.287458646308977 30.444753514104995 18.07367220776299 16-17 17.006274000480648 32.28834835786858 30.465206653406213 20.240170988244557 18-19 16.84904049210253 26.796169127008884 39.25289795417474 17.101892426713842 20-21 18.956322097684694 25.182545201668983 40.28343082712919 15.577701873517139 22-23 18.15269525377622 26.150251066136242 40.220692758454945 15.476360921632596 24-25 17.10930668971054 26.682654203887118 40.593856899610884 15.614182206791465 26-27 16.875589633193826 26.916675401973244 40.5194652458371 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17.69094283858894 30.476967208504412 34.174894794164715 17.657195158741928 54-55 17.22231028434977 30.596106744934016 34.10637677750564 18.07520619321058 56-57 17.625748457066305 30.703230062024144 33.557976979991714 18.113044500917834 58-59 17.56438903916265 30.118270278009295 33.99797513920918 18.319365543618876 60-61 17.70474870761726 30.203150806109353 34.11967131805143 17.972429168221957 62-63 17.803179440504373 29.907602943206747 34.5714300323671 17.717787583921787 64-65 17.60503965352382 29.902745322622703 34.50240068722548 17.989814336627994 66-67 17.762528826143203 30.17860703894789 33.91462859655671 18.144235538352195 68-69 17.827978871907103 30.1711927759512 33.686320429106864 18.314507923034835 70-71 17.64594593212626 30.296723918412425 33.66561162556438 18.391718523896934 72-73 17.730315131743783 29.999386405820967 33.56820354964233 18.702094912792926 74-75 17.3831230921056 30.449611134689036 32.990146700141636 19.17711907306373 76-77 17.137174092008447 30.149972643926187 33.861450434373545 18.85140282969182 78-79 17.191630575397944 30.06867141520384 33.49201560574529 19.24768240365293 80-81 16.914746202107697 30.412028491223047 33.14098860248812 19.532236704181134 82-83 16.97789526970021 30.337118868532333 32.844673746861716 19.840312114905736 84-85 16.86795964595616 30.058444845553232 32.673634369455286 20.399961139035327 86-87 16.648599726950593 29.679806104239425 32.77283209506619 20.898762073743793 88-89 16.667518880804217 29.422607877526602 32.68462793182969 21.225245309839494 90-91 16.707322062328046 29.507379337854005 32.85288372045452 20.932414879363424 92-93 16.31124258237052 29.307977674884754 33.00266663939519 21.378113103349534 94-95 15.737156706840041 29.048315428314304 33.13076203283751 22.08376583200814 96-97 16.242093583338875 29.17819286287704 32.611763623069095 21.96794993071499 98-99 16.28180479424037 29.48488505276937 32.11542788186206 22.1178822711282 100-101 16.010483922521253 29.05964329092885 32.24292015598031 22.686952630569586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1560.0 1 1070.0 2 775.5 3 947.5 4 745.0 5 615.0 6 1070.0 7 2156.0 8 3087.0 9 3271.5 10 2567.5 11 1738.5 12 1505.0 13 1494.0 14 1473.5 15 1398.0 16 1357.5 17 1333.5 18 1297.0 19 1283.0 20 1334.5 21 1431.0 22 1537.0 23 1667.0 24 1807.5 25 1995.0 26 2227.5 27 2455.5 28 2618.5 29 2902.5 30 3249.5 31 3532.0 32 3894.0 33 4400.0 34 4960.0 35 5388.0 36 5986.5 37 6438.0 38 6357.5 39 6090.0 40 5933.5 41 5954.5 42 5941.0 43 5886.5 44 5848.5 45 5790.5 46 5466.0 47 5153.0 48 5409.0 49 5211.0 50 4582.5 51 4044.5 52 3614.0 53 3329.0 54 3025.0 55 2890.0 56 2717.5 57 2433.0 58 2245.5 59 2157.0 60 1893.5 61 1517.5 62 1229.0 63 852.5 64 533.0 65 432.0 66 335.0 67 240.0 68 163.0 69 110.5 70 76.0 71 64.5 72 45.5 73 41.5 74 46.5 75 34.5 76 26.0 77 18.5 78 9.5 79 7.0 80 7.0 81 5.0 82 5.0 83 4.5 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04039495011990653 2 0.0 3 0.0 4 0.0 5 0.005624613307835087 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006135941790365548 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.113284825304623E-4 22-23 0.001533985447591387 24-25 0.0 26-27 0.001278321206326156 28-29 0.0020453139301218493 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.113284825304623E-4 38-39 0.0 40-41 2.5566424126523117E-4 42-43 0.026077752609053585 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011760555098200636 92-93 0.0023009781713870807 94-95 0.0 96-97 0.0 98-99 5.113284825304623E-4 100-101 0.016106847199709565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 195569.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.01705815639936 #Duplication Level Percentage of deduplicated Percentage of total 1 81.55914977424 44.87144486315848 2 10.508725827487062 11.563183600010236 3 3.1840543760500912 5.255319143408572 4 1.3495555814961493 2.96994311649868 5 0.7932866649723079 2.1822149290738784 6 0.5128669550017287 1.692985865389534 7 0.3432392951401255 1.3218811383600066 8 0.25942479106750915 1.1418231053898327 9 0.22051104134788918 1.0918681907368504 >10 1.1694069437791839 11.628478306946862 >50 0.06784954960421238 2.482366939946203 >100 0.021951324871951065 1.8543643350593264 >500 0.003991149976718376 1.6638775309546532 >1k 0.00498893747089797 7.152312883803423 >5k 9.97787494179594E-4 3.1279360512634815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5698 2.9135496934585747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4715 2.41091379513113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3677 1.8801548302645101 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2364 1.2087805327020131 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.6355813037853647 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.5266683370063763 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 959 0.4903640147467135 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.448946407661746 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 690 0.35281665294601905 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.25770955519535305 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.14521728903865133 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.11913953642959774 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 219 0.11198093767417128 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.10175436802356201 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.556642412652312E-4 0.0 12-13 0.0 0.0 0.0 5.113284825304624E-4 0.0 14-15 0.0 0.0 0.0 5.113284825304624E-4 0.0 16-17 0.0 0.0 0.0 0.0010226569650609249 0.0 18-19 0.0 0.0 0.0 0.0017896496888566184 0.0 20-21 0.0 0.0 0.0 0.002556642412652312 0.0 22-23 0.0 0.0 0.0 0.0035792993777132368 0.0 24-25 0.0 0.0 0.0 0.006647270272896012 0.0 26-27 0.0 0.0 0.0 0.0074142629966917045 0.0 28-29 0.0 0.0 0.0 0.011249226615670172 0.0 30-31 0.0 0.0 0.0 0.024799431402727425 0.0 32-33 0.0 0.0 0.0 0.03144670167562344 0.0 34-35 0.0 0.0 0.0 0.03655998650092806 0.0 36-37 0.0 0.0 0.0 0.04090627860243699 0.0 38-39 0.0 0.0 0.0 0.05547914035455517 0.0 40-41 0.0 0.0 0.0 0.08667017778891337 0.0 42-43 0.0 0.0 0.0 0.14138232541967286 0.0 44-45 0.0 0.0 0.0 0.18561223915855785 0.0 46-47 0.0 0.0 0.0 0.3065414252770122 0.0 48-49 0.0 0.0 0.0 0.4228686550526924 0.0 50-51 0.0 0.0 0.0 0.5182314170446236 0.0 52-53 0.0 0.0 0.0 0.6123158578302288 0.0 54-55 0.0 0.0 0.0 0.7127919046474646 0.0 56-57 0.0 0.0 0.0 0.9290838527578502 0.0 58-59 0.0 0.0 0.0 1.1880717291595293 0.0 60-61 0.0 0.0 0.0 1.4813186138907495 0.0 62-63 0.0 0.0 0.0 1.783769411307518 0.0 64-65 0.0 0.0 0.0 2.0918448220321215 0.0 66-67 0.0 0.0 0.0 2.463580628831768 0.0 68-69 0.0 0.0 0.0 2.918662978283879 0.0 70-71 0.0 0.0 0.0 3.3456222611968154 0.0 72-73 0.0 0.0 0.0 3.6884680087334907 0.0 74-75 0.0 0.0 0.0 4.065828428840971 0.0 76-77 0.0 0.0 0.0 4.517587143156636 0.0 78-79 0.0 0.0 0.0 4.981873405294295 0.0 80-81 0.0 0.0 0.0 5.49575853023741 0.0 82-83 0.0 0.0 0.0 5.9536531863434385 0.0 84-85 0.0 0.0 0.0 6.550117861215224 0.0 86-87 0.0 0.0 0.0 7.214844888504825 0.0 88-89 0.0 0.0 0.0 8.250029401387746 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1810 0.0 49.882286 1 GTATCAA 3030 0.0 38.58014 1 TGTATCG 50 0.0016133483 37.996113 9 GTATAGA 55 0.0025692512 34.559593 1 CCGGGTT 30 0.0039497702 31.66343 60-61 TAAGGTG 80 4.4828493E-4 29.684464 5 AGTATGA 165 2.6211637E-9 28.784933 6 TATCAAC 4105 0.0 28.462376 2 TAGTATG 170 3.605237E-9 27.938318 5 ATCAACG 4240 0.0 27.556143 3 GACAGGT 35 0.008389104 27.140081 56-57 ACTATCC 70 0.008379027 27.140081 8 AGCCGCC 35 0.008389104 27.140081 16-17 GGACGGG 35 0.008389104 27.140081 46-47 CGTAAGT 35 0.008389104 27.140081 62-63 TCAACGC 4320 0.0 27.045843 4 CAACGCA 4395 0.0 26.692377 5 ATGGGAT 635 0.0 26.178425 5 AACGCAG 4465 0.0 26.167536 6 ATGGGAG 500 0.0 25.647377 5 >>END_MODULE