FastQCFastQC Report
Thu 26 May 2016
SRR937884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130482
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36192.7735626369920756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27402.0999065005134807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19981.53124568906056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14561.1158627243604482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.4651982648947748No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.4514032586870219No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.37936267071320184No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.3663340537392131No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.2383470517006177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.17856869146702226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1920.14714673288269647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1320.10116337885685382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.054.1643681
GTATCAA18450.046.9388921
TATCAAC24300.035.6250532
ATCAACG24650.035.119223
ATCGTGA550.002568488734.5530137
TCAACGC25200.034.3659744
GGGATCC702.0407332E-433.9359937
CAACGCA25700.033.6973725
AACGCAG26200.033.0034036
CGACGAG300.00395603131.64923194-95
ATGGGAC2101.8189894E-1229.4565495
ACGCAGA29550.029.2618987
TCGTGAA650.005819033429.2371658
CGCAGAG29600.029.2124718
GAGACCA1004.91249E-528.5062379
GCAGAGT30000.027.8727639
TGGGACT1554.512367E-827.586686
ATGGGAG3650.027.3768985
TCCATAT700.00829314327.1906591
GTTATAA700.00829314327.1906591
Produced by FastQC (version 0.11.5)