##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937884_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 130482 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.745934305114883 33.0 31.0 34.0 30.0 34.0 2 31.73946597998191 33.0 31.0 34.0 30.0 34.0 3 31.685174966662068 33.0 31.0 34.0 30.0 34.0 4 35.39834613203354 37.0 35.0 37.0 33.0 37.0 5 35.440091353596664 37.0 35.0 37.0 33.0 37.0 6 35.56451464569826 37.0 35.0 37.0 33.0 37.0 7 35.51355742554529 37.0 35.0 37.0 33.0 37.0 8 35.53745344185405 37.0 35.0 37.0 33.0 37.0 9 37.01499823730476 39.0 37.0 39.0 33.0 39.0 10-11 36.98586395058322 39.0 37.0 39.0 33.0 39.0 12-13 36.871790745083615 39.0 37.0 39.0 33.0 39.0 14-15 37.758939930411856 40.0 37.5 41.0 32.5 41.0 16-17 37.75328014592051 40.0 37.5 41.0 32.5 41.0 18-19 37.898633527996196 40.0 37.5 41.0 33.0 41.0 20-21 38.0351121227449 40.0 38.0 41.0 33.5 41.0 22-23 37.949709538480406 40.0 37.5 41.0 33.0 41.0 24-25 37.84447280084609 40.0 37.0 41.0 33.0 41.0 26-27 37.68322067411597 40.0 37.0 41.0 33.0 41.0 28-29 37.45669900829233 40.0 36.5 41.0 33.0 41.0 30-31 36.9737243451204 39.0 35.5 40.5 32.5 41.0 32-33 36.486155178492055 38.0 35.0 40.5 31.5 41.0 34-35 36.25104995325026 38.0 35.0 41.0 31.0 41.0 36-37 35.93881531552245 38.0 35.0 41.0 30.5 41.0 38-39 35.631129197897025 38.0 35.0 40.0 30.0 41.0 40-41 35.208503854937845 38.0 35.0 40.0 28.0 41.0 42-43 34.6808946827915 38.0 35.0 40.0 24.5 41.0 44-45 34.03293174537484 38.0 34.5 40.0 21.0 41.0 46-47 33.142651860026675 38.0 33.0 40.0 5.5 41.0 48-49 32.39359068683803 37.5 33.0 40.0 2.0 41.0 50-51 31.297753713155835 36.0 31.5 39.5 2.0 40.5 52-53 31.296584969574347 36.5 31.5 39.5 2.0 40.5 54-55 31.45601692187428 37.0 32.0 40.0 2.0 41.0 56-57 31.159979920602076 36.5 31.0 40.0 2.0 41.0 58-59 30.858080041691572 36.0 31.0 40.0 2.0 41.0 60-61 30.47119142870281 35.5 30.5 40.0 2.0 41.0 62-63 30.065591422571696 35.0 29.5 39.0 2.0 41.0 64-65 29.577550926564584 35.0 28.0 39.0 2.0 41.0 66-67 29.05465888015205 35.0 27.5 39.0 2.0 40.0 68-69 28.560954767707422 35.0 26.0 38.0 2.0 40.0 70-71 28.0909205867476 35.0 25.5 37.5 2.0 40.0 72-73 27.59688692693245 34.5 24.5 37.0 2.0 39.5 74-75 27.068296776566882 34.0 21.5 36.5 2.0 39.0 76-77 26.56225762940482 34.0 20.0 36.0 2.0 39.0 78-79 26.089797826520133 34.0 18.0 35.5 2.0 38.0 80-81 25.629186401189436 33.0 10.5 35.0 2.0 37.0 82-83 25.140168758909276 33.0 4.5 35.0 2.0 37.0 84-85 24.622143284131145 33.0 2.0 35.0 2.0 36.0 86-87 24.085498382918715 32.0 2.0 35.0 2.0 36.0 88-89 23.54001318189482 32.0 2.0 35.0 2.0 35.0 90-91 22.96015159179044 31.0 2.0 35.0 2.0 35.0 92-93 22.356156404714824 31.0 2.0 34.0 2.0 35.0 94-95 21.654914087766894 30.5 2.0 34.0 2.0 35.0 96-97 20.885198724728316 29.5 2.0 34.0 2.0 35.0 98-99 19.872629941294583 29.0 2.0 34.0 2.0 35.0 100-101 17.36221087966156 22.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1288524094356447 1101 2 0.038229586496978385 1101 3 0.2511506042968925 1101 4 -0.01350587947807469 1101 5 -0.14069326749469724 1101 6 0.006634386021730165 1101 7 0.17970255587137984 1101 8 -0.088390853266894 1101 9 0.030365071299286228 1101 10-11 0.10966029617890172 1101 12-13 0.13024306876057068 1101 14-15 0.45630184646635286 1101 16-17 0.41051801713918934 1101 18-19 0.405780221790188 1101 20-21 0.31511193548950445 1101 22-23 0.2720114145199872 1101 24-25 0.30060848060065126 1101 26-27 0.5127570383482762 1101 28-29 0.43382582613651977 1101 30-31 0.7038528216249418 1101 32-33 0.6931966550302775 1101 34-35 0.656730902719552 1101 36-37 0.8201670261106102 1101 38-39 0.9280016914577587 1101 40-41 1.005024794508543 1101 42-43 1.153103701061383 1101 44-45 1.713362617141776 1101 46-47 1.982153868361145 1101 48-49 2.5404128252062286 1101 50-51 2.2311304044645226 1101 52-53 2.919153618646604 1101 54-55 3.1708651672245765 1101 56-57 3.611681124606335 1101 58-59 3.7295527145957017 1101 60-61 4.029627104313487 1101 62-63 4.282878757946854 1101 64-65 4.095532377209569 1101 66-67 3.926707510506361 1101 68-69 3.8660520891000587 1101 70-71 4.477282337613378 1101 72-73 4.471279751944934 1101 74-75 4.112308700349352 1101 76-77 3.658412646771044 1101 78-79 3.904286981731385 1101 80-81 4.068013115252885 1101 82-83 3.9574408598723068 1101 84-85 3.750067833810533 1101 86-87 4.0975333884158545 1101 88-89 3.790469110246189 1101 90-91 3.0897648975468464 1101 92-93 3.07245878739754 1101 94-95 3.152877163462378 1101 96-97 3.3553951820329644 1101 98-99 3.9650339391782126 1101 100-101 4.509914953163177 1102 1 0.14218136863469155 1102 2 -0.006872598140088115 1102 3 -0.147053570571277 1102 4 -0.12264052991184826 1102 5 -0.11935721247780862 1102 6 -0.46500664335787434 1102 7 -0.09858958769742543 1102 8 -0.3207545868335089 1102 9 0.041883624918881424 1102 10-11 -0.2265466003728207 1102 12-13 -0.2895280182117972 1102 14-15 -0.26361990513142075 1102 16-17 -0.32337058824099785 1102 18-19 -0.3349976450903185 1102 20-21 -0.5302016656380459 1102 22-23 -0.4757832927775283 1102 24-25 -0.16638503110015535 1102 26-27 -0.18853211622877097 1102 28-29 -0.07573651742742271 1102 30-31 0.006634042008407448 1102 32-33 0.38276677483295174 1102 34-35 -4.766985538040558E-4 1102 36-37 0.4430680491720196 1102 38-39 0.5185401959898499 1102 40-41 0.8041001511219861 1102 42-43 0.8256450730815033 1102 44-45 1.3475817126680099 1102 46-47 1.2261698096505356 1102 48-49 1.7151594899288334 1102 50-51 1.8562670961609555 1102 52-53 1.5711487098959083 1102 54-55 1.6484902553147833 1102 56-57 1.4356647459035052 1102 58-59 1.897367591512424 1102 60-61 2.060379698418128 1102 62-63 1.7885104558399831 1102 64-65 2.072398066986242 1102 66-67 2.6000690327050933 1102 68-69 2.7676583684128353 1102 70-71 2.9037968816098783 1102 72-73 2.747797941053335 1102 74-75 2.839873036574474 1102 76-77 2.799569605289669 1102 78-79 2.4823045268700206 1102 80-81 2.3845303323314475 1102 82-83 2.614867167859959 1102 84-85 2.8798398928127433 1102 86-87 2.773063752198027 1102 88-89 3.1450169948662428 1102 90-91 3.412290474082045 1102 92-93 3.325751538485001 1102 94-95 3.1903367634720965 1102 96-97 3.375358244898994 1102 98-99 3.7268501769367113 1102 100-101 3.402918768380305 1103 1 -0.0314809493838375 1103 2 -0.4500187505318465 1103 3 -0.28407380123869785 1103 4 -0.13568647432477832 1103 5 -0.0604424837956401 1103 6 -0.2792437392172076 1103 7 -0.3019550632126453 1103 8 -0.33807355072082146 1103 9 -0.6365054845536093 1103 10-11 -0.13508318573867228 1103 12-13 -0.30092903919874914 1103 14-15 -0.32429300023446217 1103 16-17 0.06196690466881449 1103 18-19 -0.14829883624176432 1103 20-21 0.028637434380861748 1103 22-23 -0.017643757893807788 1103 24-25 0.10307044252434139 1103 26-27 0.13212396242504099 1103 28-29 -0.053199175774942375 1103 30-31 0.14914680434532812 1103 32-33 0.5774538612387019 1103 34-35 0.7849364230192677 1103 36-37 0.8446184367689185 1103 38-39 1.1692586682908512 1103 40-41 1.0294073925664975 1103 42-43 0.45556566298507306 1103 44-45 0.7320872902819247 1103 46-47 1.0786750091211346 1103 48-49 0.8239513220331993 1103 50-51 0.5330112822074682 1103 52-53 0.5953215213914689 1103 54-55 0.7504660541425388 1103 56-57 0.838151038208288 1103 58-59 1.2975472095876732 1103 60-61 0.7548966203315324 1103 62-63 1.4535416526635778 1103 64-65 1.6297245937398834 1103 66-67 1.7258205925462704 1103 68-69 1.5050000208991285 1103 70-71 2.2524639264198605 1103 72-73 2.6386090584668374 1103 74-75 2.155301283880199 1103 76-77 2.4958543472068584 1103 78-79 2.220572630481289 1103 80-81 2.848493274286451 1103 82-83 2.4556593781076437 1103 84-85 2.37052811364844 1103 86-87 2.3269775355219053 1103 88-89 2.2218935310734658 1103 90-91 2.452269676199645 1103 92-93 2.6044337090276315 1103 94-95 2.4323863002465345 1103 96-97 2.6575666018662822 1103 98-99 2.3512026065075204 1103 100-101 2.397322247298815 1104 1 -0.5095885015436963 1104 2 -0.3998865142697241 1104 3 -0.6849259904834817 1104 4 -0.5359215610129908 1104 5 -0.2857436886149145 1104 6 -0.49302569631388593 1104 7 -0.541243925980794 1104 8 -0.6978443411998114 1104 9 -0.15354926944928593 1104 10-11 -0.7114863594089655 1104 12-13 -0.6407468470153859 1104 14-15 -0.552753182426649 1104 16-17 -0.8351385904825221 1104 18-19 -0.5981665999796419 1104 20-21 -0.8615036176320672 1104 22-23 -0.16764375789380637 1104 24-25 -0.7893186259446523 1104 26-27 -1.0341704836519483 1104 28-29 -1.0965873626688634 1104 30-31 -0.8832510798744764 1104 32-33 -1.224382753512991 1104 34-35 -0.8774497068661233 1104 36-37 -1.1114056596166293 1104 38-39 -0.5247025424055991 1104 40-41 -0.6417063306189235 1104 42-43 -1.2741728030742792 1104 44-45 -0.746328813156218 1104 46-47 0.7827449474108832 1104 48-49 0.9174570522712209 1104 50-51 0.9263847761833688 1104 52-53 1.0220920180120956 1104 54-55 1.0054763391851473 1104 56-57 0.9412218580731135 1104 58-59 0.9919785936605514 1104 60-61 1.3253932409603877 1104 62-63 1.2333829691490337 1104 64-65 1.5000155135165514 1104 66-67 1.2914391643946423 1104 68-69 1.29934324746392 1104 70-71 1.2934718605955844 1104 72-73 1.2241218413055108 1104 74-75 1.1904614366865474 1104 76-77 0.884467335746379 1104 78-79 1.0108606116743566 1104 80-81 1.4995952431374668 1104 82-83 0.49800436782260604 1104 84-85 0.589305662869716 1104 86-87 1.144036013335601 1104 88-89 1.4578030226985028 1104 90-91 1.3275561881009104 1104 92-93 1.066481901798717 1104 94-95 0.7779196531704287 1104 96-97 1.1023359230534737 1104 98-99 1.5856574992492192 1104 100-101 2.211221747739602 1105 1 -0.012484701729054137 1105 2 0.2917260899934817 1105 3 0.18173295298268854 1105 4 0.42364748439942446 1105 5 0.18378828543512782 1105 6 0.5441615140660971 1105 7 0.5563000962620208 1105 8 0.14518160875384467 1105 9 0.775783446028008 1105 10-11 0.3984533996271793 1105 12-13 0.5413082966361458 1105 14-15 0.4702426470450831 1105 16-17 0.372403114800143 1105 18-19 0.5501767735143304 1105 20-21 0.12673780961538483 1105 22-23 -0.09072068097072616 1105 24-25 0.18670083652059333 1105 26-27 0.7373772457271031 1105 28-29 0.5465428504914769 1105 30-31 1.1043531833322007 1105 32-33 0.8509763753099975 1105 34-35 1.485733796377616 1105 36-37 1.9138492059996892 1105 38-39 1.5589397189475065 1105 40-41 1.478891445099336 1105 42-43 1.8372729800582448 1105 44-45 2.5120122433776118 1105 46-47 2.072249117939144 1105 48-49 1.9580038548662166 1105 50-51 2.3089728206690054 1105 52-53 2.6249650485959677 1105 54-55 3.514396635756043 1105 56-57 2.9891829331426223 1105 58-59 3.037275164559535 1105 60-61 3.8474622863728065 1105 62-63 3.956332459417794 1105 64-65 3.68983247366484 1105 66-67 3.424085132883988 1105 68-69 3.287410902700252 1105 70-71 4.307084001466766 1105 72-73 3.31309311099967 1105 74-75 3.113017043730107 1105 76-77 3.282463165218111 1105 78-79 3.452936607967221 1105 80-81 3.3014951504590577 1105 82-83 3.147216601372186 1105 84-85 3.7886800836296786 1105 86-87 3.5062927325200306 1105 88-89 3.95815056672075 1105 90-91 4.398165548619907 1105 92-93 4.453472170566094 1105 94-95 3.086654592929463 1105 96-97 3.1282279527105636 1105 98-99 4.040766396376188 1105 100-101 3.987519245422641 1106 1 -0.029014520225164375 1106 2 0.31095685922425176 1106 3 0.18789112065771008 1106 4 0.04929571257574139 1106 5 0.28113054991222697 1106 6 -0.15235966138766344 1106 7 0.357294046132381 1106 8 -0.0945547784544516 1106 9 0.5218940769700993 1106 10-11 -0.021490420597537252 1106 12-13 -0.11929023404665884 1106 14-15 -0.3207577851070269 1106 16-17 -0.48936222660002926 1106 18-19 -0.016536709631736812 1106 20-21 -0.1041934781979279 1106 22-23 -0.40078982530953766 1106 24-25 -0.2933121280429347 1106 26-27 0.3510332526415354 1106 28-29 0.48771614349060854 1106 30-31 0.6988864590452479 1106 32-33 0.498610342466435 1106 34-35 0.7349775301719106 1106 36-37 1.0659667513756617 1106 38-39 0.8463208771151827 1106 40-41 1.0831481434571515 1106 42-43 1.3063741036537522 1106 44-45 0.41737092963517597 1106 46-47 1.0900753927878952 1106 48-49 1.1276235610027747 1106 50-51 1.7543595968142078 1106 52-53 1.7948008307913028 1106 54-55 1.4073309486341792 1106 56-57 1.092467289235966 1106 58-59 1.5067004022431938 1106 60-61 2.472408267358116 1106 62-63 2.761269797360754 1106 64-65 2.5256686880122885 1106 66-67 2.4890159885451766 1106 68-69 2.585487825777175 1106 70-71 2.6555606652524197 1106 72-73 2.5451587981215376 1106 74-75 3.0044928432115228 1106 76-77 3.3355638566615013 1106 78-79 2.9959357436629865 1106 80-81 2.47197916083071 1106 82-83 1.9523159963592214 1106 84-85 2.6077163844075564 1106 86-87 2.6209210816989383 1106 88-89 3.136089201120054 1106 90-91 3.279701849397785 1106 92-93 2.832523596668082 1106 94-95 2.315587177199127 1106 96-97 2.7807236311893853 1106 98-99 3.205902524293215 1106 100-101 4.21839651422126 1107 1 0.15045708621273235 1107 2 0.20284875111614298 1107 3 0.11546476171449882 1107 4 0.19885538460732732 1107 5 0.8773953540421999 1107 6 0.08496192986650897 1107 7 0.7397720297339561 1107 8 0.7982481368203764 1107 9 0.746157666402226 1107 10-11 0.2734533996271793 1107 12-13 -0.007158439330595456 1107 14-15 0.6013497156521552 1107 16-17 -0.10589730100027595 1107 18-19 0.4032173140548707 1107 20-21 0.145578765956337 1107 22-23 0.26478867453862875 1107 24-25 0.6672101920299482 1107 26-27 0.3959011542510069 1107 28-29 0.4726083390569684 1107 30-31 0.6695818735608867 1107 32-33 0.8615013233349416 1107 34-35 0.63256331820714 1107 36-37 0.6378616800121577 1107 38-39 0.2907484507562472 1107 40-41 0.8512926925005786 1107 42-43 1.3372729800582448 1107 44-45 1.0613885427539103 1107 46-47 1.1624258331158615 1107 48-49 0.9943863912487565 1107 50-51 0.7857139849101671 1107 52-53 0.074029497660419 1107 54-55 -0.14178839542898558 1107 56-57 -0.38399794003824894 1107 58-59 0.18670343898780573 1107 60-61 1.1682782946888146 1107 62-63 1.3722628128481524 1107 64-65 1.3084655297978962 1107 66-67 0.71540529920415 1107 68-69 1.4976499879393366 1107 70-71 1.2804467873295486 1107 72-73 0.7429267908333514 1107 74-75 0.9735939668070301 1107 76-77 0.5115432067981551 1107 78-79 1.0496102046408176 1107 80-81 1.637253986217896 1107 82-83 1.4689421730977585 1107 84-85 1.7520376719872672 1107 86-87 1.2887771400044379 1107 88-89 0.3658948494650325 1107 90-91 0.31591502886938727 1107 92-93 -0.1482689936750674 1107 94-95 0.8873302686051403 1107 96-97 0.5288256658082737 1107 98-99 0.7150127581225512 1107 100-101 1.630710513613911 1108 1 -0.1112331754775262 1108 2 -0.5779320296646375 1108 3 -0.4008372545875183 1108 4 -0.561547875795938 1108 5 -0.42869645204960705 1108 6 -0.20568586078127993 1108 7 -0.12959733963540998 1108 8 -0.260953922381681 1108 9 -0.0670126467680916 1108 10-11 -0.11146723529345337 1108 12-13 0.11800243583028447 1108 14-15 0.20795632225875949 1108 16-17 -0.7001701452731126 1108 18-19 0.08886724970480486 1108 20-21 -0.9078317874542137 1108 22-23 -0.44145328170333187 1108 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0.3624828556959194 2313 76-77 0.32110276635771484 2313 78-79 0.44225284728345926 2313 80-81 -0.335969237005326 2313 82-83 0.348071302226888 2313 84-85 1.0846202545698524 2313 86-87 1.3428311940584905 2313 88-89 1.1579368915070738 2313 90-91 1.8306797936341503 2313 92-93 1.8229522275461534 2313 94-95 1.6283212595961274 2313 96-97 2.419716556699168 2313 98-99 3.0868846299944224 2313 100-101 2.345425228328624 2314 1 0.020531980384014048 2314 2 0.08204119657364828 2314 3 -0.028299484459385127 2314 4 0.2592591618785747 2314 5 0.6118466728308647 2314 6 0.7298658699511762 2314 7 0.7057440258264336 2314 8 0.5852881889206714 2314 9 0.5512700076591415 2314 10-11 0.04152568878380691 2314 12-13 0.19660255057497977 2314 14-15 0.703270913958896 2314 16-17 0.451005987830726 2314 18-19 0.992194845803489 2314 20-21 0.8643999968257603 2314 22-23 0.7811514228290832 2314 24-25 0.6263440246577545 2314 26-27 0.5923355404444379 2314 28-29 0.502810227692585 2314 30-31 0.7221706068725169 2314 32-33 1.1280268850412298 2314 34-35 1.056285232892911 2314 36-37 0.9518473524315638 2314 38-39 0.45421312024500793 2314 40-41 1.0088960790196282 2314 42-43 0.21980310054017593 2314 44-45 0.503671186843782 2314 46-47 0.5206967546397934 2314 48-49 -0.20302487543962044 2314 50-51 0.20650525811107912 2314 52-53 -0.495980271144532 2314 54-55 0.10788597773936459 2314 56-57 0.03459535204901698 2314 58-59 -0.7098286352551106 2314 60-61 -0.34930555422033294 2314 62-63 0.08880465589601982 2314 64-65 -0.018056775640072686 2314 66-67 -0.11819938982222311 2314 68-69 0.47102999445187166 2314 70-71 0.9290140292702844 2314 72-73 0.35665196178743486 2314 74-75 0.4946783041564302 2314 76-77 0.6766360104451756 2314 78-79 0.5921859128791773 2314 80-81 0.4092337973543323 2314 82-83 0.7630646087864612 2314 84-85 0.48087192792995737 2314 86-87 0.44222878441993885 2314 88-89 0.2047909745057339 2314 90-91 0.6950260676189792 2314 92-93 1.6538312993890827 2314 94-95 2.184546159194525 2314 96-97 1.0812515857040772 2314 98-99 0.2934888245504226 2314 100-101 0.4039928320769537 2315 1 -0.1299921942365465 2315 2 -0.2734587251913325 2315 3 -0.511948365698629 2315 4 -0.1474064616545192 2315 5 -0.5903126136657662 2315 6 0.1262044711090553 2315 7 0.06953685949878974 2315 8 0.11134044991268865 2315 9 -0.47971105946650283 2315 10-11 -0.2297933536195771 2315 12-13 -0.847365529537683 2315 14-15 -0.6196050053025672 2315 16-17 -0.539996985099954 2315 18-19 -0.7444660836285308 2315 20-21 0.29563141600899456 2315 22-23 0.19923935898930978 2315 24-25 -0.03794951312976025 2315 26-27 -0.7326527243028664 2315 28-29 -0.7784446619960406 2315 30-31 0.11921332319233358 2315 32-33 -0.44316698133335564 2315 34-35 -1.1242811886373687 2315 36-37 -1.2774594853090093 2315 38-39 -0.9145867545789557 2315 40-41 -1.4080965668886734 2315 42-43 -2.075064682279418 2315 44-45 -3.7277479963826323 2315 46-47 -3.8127408920508614 2315 48-49 -4.663125016262651 2315 50-51 -4.803352354156171 2315 52-53 -4.507402583771665 2315 54-55 -5.4200439236845135 2315 56-57 -5.623204679244992 2315 58-59 -6.823738821454452 2315 60-61 -6.4808968544863355 2315 62-63 -6.03261609203075 2315 64-65 -5.678986207653846 2315 66-67 -6.144721060922208 2315 68-69 -5.336590096300746 2315 70-71 -4.982189225306463 2315 72-73 -4.679414381507822 2315 74-75 -5.0036787604656965 2315 76-77 -4.740826045571097 2315 78-79 -5.478382073351462 2315 80-81 -5.754199155235245 2315 82-83 -5.624511670356085 2315 84-85 -5.970935300985705 2315 86-87 -5.7058701046428055 2315 88-89 -5.345670612100431 2315 90-91 -5.104144641190281 2315 92-93 -5.6241377195437945 2315 94-95 -5.427955796680926 2315 96-97 -6.0259303889477795 2315 98-99 -5.481898438788647 2315 100-101 -4.063304930401534 2316 1 -0.2324800678087584 2316 2 -0.12277808053478267 2316 3 0.03194147939603553 2316 4 0.199018198023154 2316 5 -0.19538224283178351 2316 6 0.06721526754153473 2316 7 0.16357535112764054 2316 8 0.38046891181223685 2316 9 0.0813904895868589 2316 10-11 0.15598352010911043 2316 12-13 -0.7070119072563514 2316 14-15 -1.6943194474868832 2316 16-17 -0.952608470000591 2316 18-19 -0.4355159975700076 2316 20-21 -0.2380096417284534 2316 22-23 -0.2549931554841649 2316 24-25 -0.22907766208923164 2316 26-27 -0.48597771256761746 2316 28-29 -0.6538162783315116 2316 30-31 -0.783853489513028 2316 32-33 -0.9322140788141908 2316 34-35 -0.6123894659022753 2316 36-37 -0.7770683102190361 2316 38-39 -0.8409676026465505 2316 40-41 -0.2862846438719373 2316 42-43 -0.5030884657248862 2316 44-45 -0.9722324276140526 2316 46-47 -0.40098999234815835 2316 48-49 -1.112663429656486 2316 50-51 -0.8718079949009692 2316 52-53 -0.06224533138549759 2316 54-55 0.2042715199080405 2316 56-57 -0.03166970819194859 2316 58-59 -0.011033454532221043 2316 60-61 0.9458751686712326 2316 62-63 0.8839853787875853 2316 64-65 -0.2228760527485072 2316 66-67 -0.7688017994607783 2316 68-69 -0.4024639814517421 2316 70-71 -0.2456847659104362 2316 72-73 -1.1794926165258133 2316 74-75 -0.6499000090965836 2316 76-77 -1.1607133871451865 2316 78-79 -0.5584164967593814 2316 80-81 0.19236632747481508 2316 82-83 0.034148946135854175 2316 84-85 0.13147433756851257 2316 86-87 -0.4794579625680164 2316 88-89 -0.481956013446073 2316 90-91 -1.0760582697304137 2316 92-93 -0.9244819536229656 2316 94-95 -0.09256227454041621 2316 96-97 0.6595648387161255 2316 98-99 0.9802358125022295 2316 100-101 1.3377277718359881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 610.0 3 110.0 4 98.0 5 105.0 6 103.0 7 139.0 8 197.0 9 267.0 10 340.0 11 529.0 12 933.0 13 1281.0 14 1532.0 15 2087.0 16 2844.0 17 3955.0 18 3226.0 19 1819.0 20 1684.0 21 1689.0 22 1699.0 23 1802.0 24 1937.0 25 2010.0 26 2064.0 27 2319.0 28 2498.0 29 2930.0 30 3350.0 31 4196.0 32 5062.0 33 6437.0 34 8623.0 35 12478.0 36 14421.0 37 19020.0 38 14530.0 39 1558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.47011109829701 20.28216660900319 15.731365086687427 16.516357206012376 2 15.014177366928699 23.178650191721033 41.95962716214451 19.84754527920576 3 18.339775757529182 32.122464976522934 31.101923505498476 18.435835760449407 4 10.378772160361482 19.551067770170057 38.927695937171 31.142464132297455 5 10.115960071005372 41.166977891509326 32.53337021924061 16.183691818244693 6 24.490391557474002 40.06359544693467 18.550208144518347 16.89580485107298 7 23.243222486752167 40.185853621073655 20.57445664695492 15.996467245219263 8 26.513621247148638 33.5750109446164 19.5682060813665 20.34316172686846 9 28.93855606758833 16.817972350230413 21.117511520737327 33.125960061443934 10-11 23.294278483734825 30.683594275027552 24.84879206460755 21.17333517663007 12-13 26.293212697486158 29.784022918762815 27.255201652854478 16.667562730896552 14-15 22.94650091974946 28.457701125589 30.07791948447947 18.517878470182065 16-17 16.862695404748465 33.01154668786312 29.78907230983676 20.336685597551657 18-19 16.73987666930325 27.777436472404183 38.02862869473733 17.454058163555242 20-21 19.10051528578779 26.25154547339482 38.59767007886714 16.050269161950254 22-23 18.205555512890417 27.249967361169773 38.55797808206554 15.986499043874266 24-25 16.799975424597672 27.90191342546549 39.07719364265061 16.220917507286224 26-27 16.424358324501178 28.336635792513405 39.05042778366588 16.18857809931954 28-29 16.296717307485522 28.417103181308466 39.188005960153 16.098173551053012 30-31 16.19424336104764 28.702932083949385 38.91126941761554 16.191555137387432 32-33 15.651652989358967 29.17778707945639 38.428690472990354 16.741869458194287 34-35 16.064886287702482 29.38124534359471 38.25164942356582 16.302218945136985 36-37 16.363964489770844 29.2844197333661 37.97344412361 16.378171653253055 38-39 16.05247799978499 29.846574416783128 37.765116029057175 16.335831554374703 40-41 16.06559391209863 29.695412956755778 37.698837785226395 16.540155345919196 42-43 16.087315452992108 29.93683644671415 36.82492752510223 17.150920575191506 44-45 16.067794527596245 30.283296343795357 36.4042329342559 17.244676194352504 46-47 16.46781433964003 30.600857874667355 35.41747468424913 17.513853101443488 48-49 16.53501276857203 31.050479061461957 34.42330216393694 17.99120600602907 50-51 16.792878867008696 31.328530613498817 33.78198826456945 18.096602254923045 52-53 16.90131649691998 31.7510791587938 33.12902284283454 18.21858150145168 54-55 16.702954898911354 31.63181844363804 33.19701246087975 18.46821419657086 56-57 16.8135783225965 31.880725139553277 32.63780646052965 18.667890077320575 58-59 17.070586880589566 31.288911065904117 33.036118681149965 18.604383372356352 60-61 17.154045493809384 31.201458873212403 33.31605720318649 18.328438429791728 62-63 17.400133590277083 30.71347956606193 33.66052714416233 18.225859699498653 64-65 17.317644348894348 30.58776105651106 33.81065724815725 18.283937346437344 66-67 17.29253450559576 30.75537980150884 33.45900600848483 18.493079684410574 68-69 17.006280423199176 30.39632695053975 33.48317798627213 19.114214639988944 70-71 16.997857751637397 30.377312130961247 33.614103518969266 19.01072659843209 72-73 17.11123995438436 30.31980862934222 33.35125193424898 19.217699482024443 74-75 16.758375731976578 30.52433522127292 33.170394547374485 19.54689449937602 76-77 16.615214486476525 30.366385599533093 33.36251516687401 19.655884747116374 78-79 16.747557903790625 30.30157584321435 33.10384284573325 19.84702340726178 80-81 16.430724337192114 30.328092303557376 33.09451131716869 20.14667204208182 82-83 16.673513185627687 29.917496247211805 33.018393461126486 20.390597106034022 84-85 16.781251799389647 29.917276059960464 32.651964453657314 20.64950768699257 86-87 16.922616213891917 29.719581676060013 32.34562403059109 21.012178079456977 88-89 17.131261470884112 29.317084034065168 32.38686540573333 21.16478908931739 90-91 17.44377126095203 29.219747057061902 32.48865442650142 20.847827255484656 92-93 17.451712845477267 29.28556894572566 32.36880597989043 20.893912228906643 94-95 17.32829116596921 29.19030982454793 32.16070948669712 21.32068952278573 96-97 18.076928983904654 29.229635090305933 31.77448089445878 20.91895503133063 98-99 18.302834368736708 29.842654523394486 30.716924045675498 21.137587062193315 100-101 19.099842616406278 28.836512993743042 30.94238225020153 21.12126213964915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 690.0 1 485.0 2 382.0 3 467.0 4 418.5 5 408.0 6 640.5 7 1225.5 8 1847.0 9 2000.5 10 1565.0 11 1121.5 12 1031.5 13 1025.0 14 995.0 15 978.0 16 936.5 17 925.5 18 916.5 19 907.5 20 947.5 21 1018.0 22 1150.5 23 1236.0 24 1304.5 25 1448.0 26 1602.5 27 1767.5 28 1914.0 29 2055.0 30 2282.0 31 2505.0 32 2804.5 33 3135.0 34 3457.0 35 3729.5 36 4091.0 37 4438.0 38 4370.0 39 4168.5 40 4043.0 41 4061.5 42 3972.0 43 3879.0 44 3860.5 45 3768.0 46 3542.0 47 3390.5 48 3648.5 49 3506.0 50 2949.0 51 2571.5 52 2323.5 53 2201.0 54 2095.5 55 1951.5 56 1764.5 57 1507.5 58 1370.0 59 1296.0 60 1113.0 61 944.5 62 773.0 63 541.5 64 363.0 65 265.0 66 205.0 67 147.0 68 88.5 69 68.5 70 53.0 71 37.0 72 29.5 73 23.5 74 18.0 75 16.0 76 14.5 77 12.0 78 6.5 79 4.5 80 6.0 81 4.0 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31958431047960634 2 0.2644042856485952 3 0.27206817798623567 4 0.2690026210511795 5 0.2690026210511795 6 0.21765454238898851 7 0.20845787158382 8 0.2153553746876964 9 0.21612176392146043 10-11 0.21497218007081437 12-13 0.21688815315522447 14-15 0.21727134777210652 16-17 0.20807467696693796 18-19 0.20156036847994357 20-21 0.20156036847994357 22-23 0.20615870388252786 24-25 0.20730828773317395 26-27 0.21152342851887615 28-29 0.21765454238898851 30-31 0.21803773700587054 32-33 0.21343940160328628 34-35 0.21842093162275258 36-37 0.20385953618123573 38-39 0.19619564384359528 40-41 0.1965788384604773 42-43 0.20270995233058967 44-45 0.20309314694747166 46-47 0.21190662313575817 48-49 0.21420579083705032 50-51 0.21305620698640423 52-53 0.22072009932404468 54-55 0.2103738446682301 56-57 0.18776536227219082 58-59 0.16630646372679758 60-61 0.18738216765530877 62-63 0.17856869146702226 64-65 0.18546619457089866 66-67 0.191214113824129 68-69 0.1808678591683144 70-71 0.18776536227219082 72-73 0.20194356309682562 74-75 0.20654189849940988 76-77 0.20232675771370764 78-79 0.20385953618123573 80-81 0.19887800616176943 82-83 0.1881485568890728 84-85 0.1758863291488481 86-87 0.19006452997348292 88-89 0.2000275900124155 90-91 0.19542925460983124 92-93 0.1881485568890728 94-95 0.18929814073971887 96-97 0.19772842231112336 98-99 0.19849481154488743 100-101 0.173970356064438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 130482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.02582731717786 #Duplication Level Percentage of deduplicated Percentage of total 1 86.69015993494172 56.3709937002805 2 7.684420191639068 9.993715608283136 3 2.178038115667024 4.24886191198786 4 1.0465897438919467 2.7222145583298847 5 0.5999033554515776 1.9504605999294922 6 0.3641849446651031 1.4208856393985378 7 0.2805049088359046 1.2768044634508975 8 0.1873961365752472 0.9748471053478641 9 0.16500288755053213 0.9656504345426956 >10 0.7495845463009888 8.263975107677688 >50 0.033000577510106426 1.4331478671387625 >100 0.014143104647188468 1.9412639291243237 >500 0.002357184107864745 0.9166015235817967 >1k 0.00471436821572949 7.520577550926564 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3619 2.7735626369920756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2740 2.0999065005134807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 1.53124568906056 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1456 1.1158627243604482 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 607 0.4651982648947748 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 589 0.4514032586870219 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.37936267071320184 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.3663340537392131 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.2383470517006177 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.17856869146702226 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 192 0.14714673288269647 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 132 0.10116337885685382 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.663892337640441E-4 0.0 10-11 0.0 0.0 0.0 0.0011495838506460662 0.0 12-13 0.0 0.0 0.0 0.0015327784675280881 0.0 14-15 0.0 0.0 0.0 0.0015327784675280881 0.0 16-17 0.0 0.0 0.0 0.0019159730844101103 0.0 18-19 0.0 0.0 0.0 0.0026823623181741543 0.0 20-21 0.0 0.0 0.0 0.0030655569350561762 0.0 22-23 0.0 0.0 0.0 0.0038319461688202205 0.0 24-25 0.0 0.0 0.0 0.005364724636348309 0.0 26-27 0.0 0.0 0.0 0.008430281571404484 0.0 28-29 0.0 0.0 0.0 0.014178200824634815 0.0 30-31 0.0 0.0 0.0 0.026823623181741543 0.0 32-33 0.0 0.0 0.0 0.039085850921966245 0.0 34-35 0.0 0.0 0.0 0.04713293787648871 0.0 36-37 0.0 0.0 0.0 0.05249766251283702 0.0 38-39 0.0 0.0 0.0 0.06705905795435385 0.0 40-41 0.0 0.0 0.0 0.10001379500620775 0.0 42-43 0.0 0.0 0.0 0.16285771217485936 0.0 44-45 0.0 0.0 0.0 0.2119066231357582 0.0 46-47 0.0 0.0 0.0 0.3425759874925277 0.0 48-49 0.0 0.0 0.0 0.48550757958952195 0.0 50-51 0.0 0.0 0.0 0.6035315215891848 0.0 52-53 0.0 0.0 0.0 0.7456967244524149 0.0 54-55 0.0 0.0 0.0 0.8671694180040159 0.0 56-57 0.0 0.0 0.0 1.1361720390551953 0.0 58-59 0.0 0.0 0.0 1.4710841342100827 0.0 60-61 0.0 0.0 0.0 1.8228567925077788 0.0 62-63 0.0 0.0 0.0 2.1796109808249415 0.0 64-65 0.0 0.0 0.0 2.5972931132263453 0.0 66-67 0.0 0.0 0.0 3.098128477491148 0.0 68-69 0.0 0.0 0.0 3.6890145767232263 0.0 70-71 0.0 0.0 0.0 4.268404837448843 0.0 72-73 0.0 0.0 0.0 4.75851075244095 0.0 74-75 0.0 0.0 0.0 5.2777394583160895 0.0 76-77 0.0 0.0 0.0 5.871691114483223 0.0 78-79 0.0 0.0 0.0 6.446866234423139 0.0 80-81 0.0 0.0 0.0 7.112475283947211 0.0 82-83 0.0 0.0 0.0 7.7478119587376035 0.0 84-85 0.0 0.0 0.0 8.537959258748334 0.0 86-87 0.0 0.0 0.0 9.403979092901704 0.0 88-89 0.0 0.0 0.0 10.706074401066815 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 940 0.0 54.164368 1 GTATCAA 1845 0.0 46.938892 1 TATCAAC 2430 0.0 35.625053 2 ATCAACG 2465 0.0 35.11922 3 ATCGTGA 55 0.0025684887 34.553013 7 TCAACGC 2520 0.0 34.365974 4 GGGATCC 70 2.0407332E-4 33.935993 7 CAACGCA 2570 0.0 33.697372 5 AACGCAG 2620 0.0 33.003403 6 CGACGAG 30 0.003956031 31.649231 94-95 ATGGGAC 210 1.8189894E-12 29.456549 5 ACGCAGA 2955 0.0 29.261898 7 TCGTGAA 65 0.0058190334 29.237165 8 CGCAGAG 2960 0.0 29.212471 8 GAGACCA 100 4.91249E-5 28.506237 9 GCAGAGT 3000 0.0 27.872763 9 TGGGACT 155 4.512367E-8 27.58668 6 ATGGGAG 365 0.0 27.376898 5 TCCATAT 70 0.008293143 27.190659 1 GTTATAA 70 0.008293143 27.190659 1 >>END_MODULE