FastQCFastQC Report
Thu 26 May 2016
SRR937884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130482
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39122.9981146824849403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31762.434052206434604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24691.8922150181634247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18361.4070906331907849No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.5993163808034825No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.5134807866219095No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.4805260495700557No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.4598335402584265No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.3180515320120783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.26363789641483115No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.1233886666360111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1550.11879033123342683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1470.11265921736331448No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.10116337885685382No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC156.604668E-495.03
TATAACG200.00206994571.252
TAACGCA250.005011329457.04
GGTATCA11550.055.519481
GTATCAA21400.042.394861
GGATTTA459.5936796E-442.222221
GTCGGGA459.5936796E-442.222222
CTGATAT704.2808206E-640.7142874
GTGGGTA500.00161056938.09
TACTGAT756.8752506E-638.02
TCGGGAG550.00257128634.5454563
CGGGAGT550.00257128634.5454564
GAGCAGA702.0434044E-433.9285746
ACACAGT600.003938236331.6666686
GGGAGTG753.063619E-431.6666685
GATATTA753.063619E-431.6666686
TATCAAC29300.030.802052
ATCAACG29300.030.6399333
TCAACGC30050.029.875214
GCTTACA650.005825347729.230772
Produced by FastQC (version 0.11.5)