##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937884_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 130482 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99616805383118 31.0 31.0 34.0 30.0 34.0 2 32.153155224475405 33.0 31.0 34.0 30.0 34.0 3 32.17422326451158 34.0 31.0 34.0 30.0 34.0 4 35.93698747719992 37.0 35.0 37.0 35.0 37.0 5 35.78814702411061 37.0 35.0 37.0 35.0 37.0 6 35.93285663922993 37.0 35.0 37.0 35.0 37.0 7 35.88034364893242 37.0 35.0 37.0 35.0 37.0 8 35.908753697828054 37.0 35.0 37.0 35.0 37.0 9 37.48144571665057 39.0 37.0 39.0 35.0 39.0 10-11 37.44178507380328 39.0 37.0 39.0 34.5 39.0 12-13 37.33033291948315 39.0 37.0 39.0 34.0 39.0 14-15 38.22774022470532 40.0 37.5 41.0 33.0 41.0 16-17 38.246524424824884 40.0 37.5 41.0 33.5 41.0 18-19 38.46706825462516 40.0 38.0 41.0 34.0 41.0 20-21 38.51113563556659 40.0 38.0 41.0 34.0 41.0 22-23 38.353251789518865 40.0 37.5 41.0 34.0 41.0 24-25 38.22124507594917 40.0 37.0 41.0 34.0 41.0 26-27 37.996988090311305 40.0 37.0 41.0 33.0 41.0 28-29 37.78024938305667 39.5 36.0 41.0 33.0 41.0 30-31 37.428403917781765 39.0 35.5 41.0 33.0 41.0 32-33 36.97969068530526 39.0 35.0 41.0 33.0 41.0 34-35 36.65967336490857 38.0 35.0 41.0 32.5 41.0 36-37 36.31418893027391 38.0 35.0 41.0 31.5 41.0 38-39 36.02324458546006 38.0 35.0 41.0 31.0 41.0 40-41 35.63842139145629 38.0 35.0 40.0 30.0 41.0 42-43 35.14671755491179 38.0 35.0 40.0 28.0 41.0 44-45 34.401292898637365 38.0 34.5 40.0 23.5 41.0 46-47 33.421257338176915 38.0 33.0 40.0 12.5 41.0 48-49 32.58277003724652 37.5 33.0 40.0 2.0 41.0 50-51 32.113575052497666 37.0 33.0 40.0 2.0 41.0 52-53 31.800482058828038 37.0 33.0 40.0 2.0 41.0 54-55 31.526999892705508 36.5 32.0 40.0 2.0 41.0 56-57 31.17016140157263 36.0 31.0 40.0 2.0 41.0 58-59 30.864077037445778 36.0 31.0 40.0 2.0 41.0 60-61 30.471524808019495 35.0 30.5 39.5 2.0 41.0 62-63 30.031678698977636 35.0 29.5 39.0 2.0 41.0 64-65 29.605903496267686 35.0 29.0 39.0 2.0 40.0 66-67 29.12719378918165 35.0 28.0 38.5 2.0 40.0 68-69 28.60807237779924 35.0 26.0 38.0 2.0 40.0 70-71 28.16065434312779 35.0 26.0 37.0 2.0 40.0 72-73 27.66609953863368 34.0 25.0 37.0 2.0 39.0 74-75 27.159102404929413 34.0 23.5 36.0 2.0 39.0 76-77 25.959756901335048 32.5 20.5 35.0 2.0 37.5 78-79 26.370353765270306 33.5 20.5 35.0 2.0 38.0 80-81 26.117602427921092 34.0 19.5 35.0 2.0 37.0 82-83 25.744301896046963 33.0 17.0 35.0 2.0 37.0 84-85 25.325048665716345 33.0 9.0 35.0 2.0 36.0 86-87 24.84841587345381 33.0 3.5 35.0 2.0 36.0 88-89 24.36279716742539 32.5 2.0 35.0 2.0 35.5 90-91 23.807099829861592 32.0 2.0 35.0 2.0 35.0 92-93 23.394318756610108 32.0 2.0 35.0 2.0 35.0 94-95 22.917387072546404 31.0 2.0 34.0 2.0 35.0 96-97 22.34360294906577 31.0 2.0 34.0 2.0 35.0 98-99 21.625231832743214 31.0 2.0 34.0 2.0 35.0 100-101 19.724674667770266 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24050135562005792 1101 2 0.08413720205272313 1101 3 0.2284980079512664 1101 4 0.07137760791734848 1101 5 0.0297318942720608 1101 6 0.01372643213504432 1101 7 -0.09414835825831602 1101 8 -0.02683873273908688 1101 9 -0.06439574115858449 1101 10-11 -0.003952912712463785 1101 12-13 0.1758117760166229 1101 14-15 0.034829744478713565 1101 16-17 -0.16830884220604503 1101 18-19 0.007286387846114906 1101 20-21 0.2921955663113138 1101 22-23 -0.19966139802992444 1101 24-25 -0.17211813897424122 1101 26-27 -0.10859352342010453 1101 28-29 0.4171181758078717 1101 30-31 0.6024920610323079 1101 32-33 0.637779771609182 1101 34-35 0.8929317099132206 1101 36-37 1.2146687426148333 1101 38-39 1.118193439680887 1101 40-41 1.207600307676941 1101 42-43 1.5917709966171927 1101 44-45 1.4638327204222037 1101 46-47 1.7233205203550739 1101 48-49 2.0689797440365254 1101 50-51 2.072335731480596 1101 52-53 2.4210158664901904 1101 54-55 2.42674550988097 1101 56-57 2.33037288842921 1101 58-59 2.4239950505294026 1101 60-61 2.5121805103667008 1101 62-63 2.5862669416427195 1101 64-65 3.1461644101706767 1101 66-67 3.1253336672501497 1101 68-69 3.4308777417686684 1101 70-71 3.356667985516978 1101 72-73 3.194126371362966 1101 74-75 3.2665677471647108 1101 76-77 3.44602409709454 1101 78-79 3.7600226574477595 1101 80-81 3.925134112818302 1101 82-83 3.7389296178822136 1101 84-85 3.9629570584458307 1101 86-87 4.176099288001613 1101 88-89 4.308559508130838 1101 90-91 4.529010836647274 1101 92-93 4.482120455437638 1101 94-95 4.353118234617803 1101 96-97 4.333320737363412 1101 98-99 4.311897634055438 1101 100-101 4.786088831026365 1102 1 0.060335624532118715 1102 2 -0.030878228921004336 1102 3 -0.012249482996701033 1102 4 0.011925832747849086 1102 5 0.01974428765254288 1102 6 -0.010331306437528553 1102 7 0.05363662874078301 1102 8 -0.16962991642842695 1102 9 -0.05924398299961098 1102 10-11 -0.1362826252346423 1102 12-13 0.06725426830113435 1102 14-15 -0.1920723964170108 1102 16-17 -0.2155008910019447 1102 18-19 0.0372674332642049 1102 20-21 -0.045396472764288376 1102 22-23 0.12059813393770469 1102 24-25 0.010963681637655043 1102 26-27 0.12469363849576354 1102 28-29 0.08999975827245521 1102 30-31 0.23568445003865435 1102 32-33 0.3030612957519736 1102 34-35 0.7485852784777762 1102 36-37 0.7702384736055734 1102 38-39 0.8022472901340976 1102 40-41 0.9103292814564341 1102 42-43 0.4951269296443357 1102 44-45 0.726286852764062 1102 46-47 1.1927441066591697 1102 48-49 1.145527094040169 1102 50-51 1.0186067820018465 1102 52-53 1.2830423057018727 1102 54-55 1.0813043298367475 1102 56-57 1.6527648108612283 1102 58-59 1.7739622445222594 1102 60-61 1.7714451697917433 1102 62-63 1.7175152709664303 1102 64-65 1.8831513606700554 1102 66-67 1.8443927423637554 1102 68-69 2.1769106449787934 1102 70-71 2.0995053406237787 1102 72-73 2.0688195744443014 1102 74-75 2.1390131312380056 1102 76-77 1.984510403624153 1102 78-79 1.9442648386827237 1102 80-81 2.3826564087527835 1102 82-83 2.3985672937588625 1102 84-85 3.0680881609706816 1102 86-87 2.5529552574798764 1102 88-89 2.5681218516947872 1102 90-91 2.3884796223389912 1102 92-93 2.405403000211763 1102 94-95 2.063787477163551 1102 96-97 2.6603237263551733 1102 98-99 3.2526038137351527 1102 100-101 3.4501553178675124 1103 1 -0.1270343887028993 1103 2 0.03680942469971882 1103 3 0.13662554063725452 1103 4 0.06893093768262304 1103 5 -0.19160001183696806 1103 6 -0.1416982945260159 1103 7 -0.11688709975231149 1103 8 0.04802937644357286 1103 9 0.011695703141441527 1103 10-11 -0.026110570025707602 1103 12-13 -0.032736278115962136 1103 14-15 0.09135547274539846 1103 16-17 -0.389541352336785 1103 18-19 -0.22895491500578657 1103 20-21 -0.09307089136893865 1103 22-23 -0.22557316498458135 1103 24-25 -0.2181155393871208 1103 26-27 0.030706117225207663 1103 28-29 -0.12955970476403422 1103 30-31 -0.3857562759965205 1103 32-33 0.260671429992847 1103 34-35 0.34331017921515894 1103 36-37 -0.03727712480622358 1103 38-39 0.01890450321784698 1103 40-41 0.40609407631368555 1103 42-43 0.5933307488918373 1103 44-45 0.9270715001454235 1103 46-47 1.2681175231840314 1103 48-49 0.8491005483793614 1103 50-51 0.35887904518959246 1103 52-53 0.7779468245145047 1103 54-55 1.1151008887325666 1103 56-57 0.8450223264596382 1103 58-59 0.3483241276759763 1103 60-61 -0.1221263938308681 1103 62-63 0.47533338403128056 1103 64-65 0.8056414344080025 1103 66-67 1.2431637765857246 1103 68-69 1.1759652866880046 1103 70-71 1.2376221869085207 1103 72-73 1.2619373660873343 1103 74-75 1.5627983458343344 1103 76-77 1.8276281952671845 1103 78-79 2.017634095631106 1103 80-81 2.0661693601613713 1103 82-83 1.4433489159936883 1103 84-85 2.0453333869113095 1103 86-87 2.5978975906241395 1103 88-89 2.598023534432542 1103 90-91 2.347659051342582 1103 92-93 2.310715913806014 1103 94-95 2.2109514022911725 1103 96-97 1.731906256133957 1103 98-99 1.7919496257979226 1103 100-101 2.3865705192288296 1104 1 -0.31745460615808696 1104 2 -0.22369601167994801 1104 3 -0.31438827070567754 1104 4 0.021032024958557827 1104 5 -0.6513120306439006 1104 6 -0.320922508454899 1104 7 -0.4182976198816135 1104 8 -0.12722774962166028 1104 9 -0.049250520778628015 1104 10-11 -0.48376558031075945 1104 12-13 -0.3003090080601254 1104 14-15 -0.3777453206721688 1104 16-17 -0.059318019982981696 1104 18-19 -0.7693310537069351 1104 20-21 -1.0593359516099028 1104 22-23 -0.8027697562276046 1104 24-25 -0.7890970263104222 1104 26-27 -1.055188681481816 1104 28-29 -0.9426657876320945 1104 30-31 -0.8898409072042739 1104 32-33 -0.751038825663926 1104 34-35 -1.3378534998915157 1104 36-37 -1.029005011964614 1104 38-39 -0.9315950559946131 1104 40-41 -0.9845759207477371 1104 42-43 -1.0805011641260975 1104 44-45 -1.0374891816059773 1104 46-47 -0.5820294059961029 1104 48-49 -0.19098173196151436 1104 50-51 0.27736273605059836 1104 52-53 0.06531091502288078 1104 54-55 -0.412227938772574 1104 56-57 -0.1080925721592294 1104 58-59 0.3073749651723041 1104 60-61 -0.11134179200894678 1104 62-63 -0.08863958099957259 1104 64-65 0.4410807526566032 1104 66-67 -0.13442952344366077 1104 68-69 0.12299731724927199 1104 70-71 0.31931481106368054 1104 72-73 0.25127030760951996 1104 74-75 0.3437857099248447 1104 76-77 -0.04388223670460789 1104 78-79 0.20133964955411798 1104 80-81 0.4612031538727983 1104 82-83 0.612772953607557 1104 84-85 0.2627738805933575 1104 86-87 -0.1440653832224683 1104 88-89 -0.45319597776257936 1104 90-91 -1.4461846160097522 1104 92-93 -1.0507004834904912 1104 94-95 -0.8961452771093548 1104 96-97 -0.2577205437484942 1104 98-99 -0.473463245198257 1104 100-101 0.25002923212921147 1105 1 -0.18191243748338692 1105 2 0.13601205134136762 1105 3 -0.04944387774924053 1105 4 -0.1374480491841723 1105 5 -0.31500526512026994 1105 6 -0.1288465121620348 1105 7 -0.2954987320224802 1105 8 -0.12908131774029385 1105 9 0.14931296392943239 1105 10-11 0.06929280708497743 1105 12-13 -0.09415747886642833 1105 14-15 -0.12670268860544098 1105 16-17 -0.2742739977401598 1105 18-19 -0.05228981181629422 1105 20-21 0.09346757016952267 1105 22-23 0.01807130030622517 1105 24-25 -0.49130972325203004 1105 26-27 -0.07644679084233275 1105 28-29 -0.1269799674281984 1105 30-31 -0.18728066624041873 1105 32-33 0.14545097971142695 1105 34-35 -0.43365980202311505 1105 36-37 0.13584670258589426 1105 38-39 0.28417185781447785 1105 40-41 0.41029201252381853 1105 42-43 0.7018204299424937 1105 44-45 0.9781502993949545 1105 46-47 1.4395762473866611 1105 48-49 1.088617433932832 1105 50-51 1.5632176943453153 1105 52-53 1.0820509520942387 1105 54-55 1.125654359651893 1105 56-57 1.7287934334014778 1105 58-59 1.936082101409557 1105 60-61 1.7326109420040297 1105 62-63 1.1290388249318468 1105 64-65 1.144220233657535 1105 66-67 1.0875352587620881 1105 68-69 1.2075084386579817 1105 70-71 1.2008022994788767 1105 72-73 1.2688792047364892 1105 74-75 0.8924418730388375 1105 76-77 0.5836084954548006 1105 78-79 1.1748104558562495 1105 80-81 1.7453203920563034 1105 82-83 1.896890191791062 1105 84-85 2.2111635932903013 1105 86-87 2.836124607509692 1105 88-89 2.6044963299297272 1105 90-91 2.0567115841756056 1105 92-93 2.956424168965487 1105 94-95 2.3671193197395795 1105 96-97 1.7342280197362108 1105 98-99 2.1780659484996114 1105 100-101 2.014220613037459 1106 1 -0.2689910891687788 1106 2 -0.2536811206551768 1106 3 -0.34471181206211554 1106 4 -0.0864540993138121 1106 5 -0.6016301570822407 1106 6 -0.42292602815166447 1106 7 -0.2159827423941323 1106 8 -0.482581749892411 1106 9 -0.345717286718795 1106 10-11 -0.911476423684249 1106 12-13 -1.1058796050548452 1106 14-15 -0.7558081337221196 1106 16-17 -0.01752162090351561 1106 18-19 -0.10938791034697459 1106 20-21 -0.6054304419307357 1106 22-23 -0.3890700134362106 1106 24-25 -0.22699468435834547 1106 26-27 0.35424681330632524 1106 28-29 -0.3870275041611251 1106 30-31 -0.5966907785999709 1106 32-33 -0.4264483288451899 1106 34-35 -1.3842864225935614 1106 36-37 -0.5939501859188567 1106 38-39 -0.9000870013039375 1106 40-41 -0.6998527584355614 1106 42-43 -0.4184258967645107 1106 44-45 0.29437761140877683 1106 46-47 0.12221453606427701 1106 48-49 0.5885634149181413 1106 50-51 0.14808156642829218 1106 52-53 0.680851729968051 1106 54-55 0.6557969698506838 1106 56-57 1.1123284377230007 1106 58-59 1.431004314028403 1106 60-61 1.3943438719953818 1106 62-63 1.3195098707399708 1106 64-65 1.2055318153342824 1106 66-67 1.0933693123489476 1106 68-69 1.0015339356329207 1106 70-71 0.18103134010117472 1106 72-73 0.6608411753501464 1106 74-75 0.8094686664701278 1106 76-77 0.8040600944176362 1106 78-79 0.6399141723644597 1106 80-81 0.5135248000079038 1106 82-83 0.684757521090976 1106 84-85 0.8743550366783701 1106 86-87 0.6599145815805656 1106 88-89 -0.08240946090864654 1106 90-91 -0.05316309171030653 1106 92-93 0.11540212920749582 1106 94-95 0.19776970867648913 1106 96-97 -0.6801518419751105 1106 98-99 -0.341813048907472 1106 100-101 0.2126324540054796 1107 1 0.2437632381922903 1107 2 -0.00406071373139838 1107 3 0.48356028025492037 1107 4 -0.10938152111764055 1107 5 -0.1260759511909555 1107 6 -0.13352426683979246 1107 7 -0.10423054075429405 1107 8 -0.23614992480889896 1107 9 0.08018614480261022 1107 10-11 0.10879384658602476 1107 12-13 0.10737578516683044 1107 14-15 -0.05887524577799752 1107 16-17 -0.062085848052014114 1107 18-19 0.1144201673936891 1107 20-21 0.2907128924148523 1107 22-23 -0.2991064543715325 1107 24-25 -0.6393086837509898 1107 26-27 -0.1958594727550178 1107 28-29 -0.5024997179479485 1107 30-31 -0.40180769326745036 1107 32-33 -0.6423608706004273 1107 34-35 -0.8216535650168808 1107 36-37 -0.7682593265201305 1107 38-39 -1.4360724249298116 1107 40-41 -1.841137301405496 1107 42-43 -1.2360693829473277 1107 44-45 -0.8355191392744814 1107 46-47 -0.40488840957799965 1107 48-49 -0.6074584496430511 1107 50-51 -0.3475151538875316 1107 52-53 -0.30191474437145516 1107 54-55 -0.41670530770777603 1107 56-57 -0.24054128593324364 1107 58-59 -0.07613203580458006 1107 60-61 -0.22295038855730098 1107 62-63 -0.5479362270432091 1107 64-65 0.832239984177285 1107 66-67 0.5137306849575118 1107 68-69 -0.0919977983482525 1107 70-71 0.0721640458406263 1107 72-73 0.2590039448612309 1107 74-75 0.29420902480599054 1107 76-77 -0.4604403610940544 1107 78-79 -0.8753454692996776 1107 80-81 -1.089269005033092 1107 82-83 -0.8228343404334701 1107 84-85 -0.592240772614069 1107 86-87 -0.10800221161712642 1107 88-89 0.08734456277796099 1107 90-91 0.19106709353111384 1107 92-93 0.41315493819625715 1107 94-95 0.5205756606959184 1107 96-97 0.35909808210627503 1107 98-99 0.28565430608797016 1107 100-101 0.9441842305010759 1108 1 -0.4557219612929124 1108 2 -0.3708560805267638 1108 3 -0.30387000717536594 1108 4 -0.17652008825621124 1108 5 -0.3349991601141653 1108 6 -0.02841916173468917 1108 7 0.1025476660239164 1108 8 0.054068865409888645 1108 9 -0.3516027869863194 1108 10-11 0.26312675091892856 1108 12-13 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0.1809034115003776 2311 76-77 -0.0846607353144293 2311 78-79 -0.41813826209247296 2311 80-81 -0.36525653102061995 2311 82-83 -1.4572764748756022 2311 84-85 -1.6622338426071366 2311 86-87 -2.558106161721078 2311 88-89 -3.030119054685656 2311 90-91 -2.38239097992696 2311 92-93 -2.2342809592396407 2311 94-95 -2.576790936670676 2311 96-97 -1.9981437751355848 2311 98-99 -1.8668058463721842 2311 100-101 -2.173279386962541 2312 1 -0.015245770816719073 2312 2 0.43152487185418664 2312 3 0.1858125325071711 2312 4 0.07665451491839548 2312 5 -0.2143642394792451 2312 6 0.19935861604309224 2312 7 0.2827063961826468 2312 8 -0.014978753637727493 2312 9 0.4198257844422528 2312 10-11 -0.07814309035092037 2312 12-13 -0.1329395301484837 2312 14-15 0.14765628575353418 2312 16-17 -0.08100476697093484 2312 18-19 -0.6468410938675717 2312 20-21 -0.7097375580356058 2312 22-23 0.06710976184469075 2312 24-25 0.2070877126454036 2312 26-27 0.29310449120894333 2312 28-29 0.08263541718719125 2312 30-31 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-1.515451467443274 2312 98-99 -1.8206520002183346 2312 100-101 -1.8546896433727973 2313 1 0.17919867362772735 2313 2 -0.1406973503680362 2313 3 0.23581253250717538 2313 4 0.2970248852887636 2313 5 -0.025475350590355106 2313 6 -0.7691599024754296 2313 7 -0.5006269371506846 2313 8 -0.4168306054895794 2313 9 0.10871467333114282 2313 10-11 -0.35592086812869184 2313 12-13 -0.08756915977810564 2313 14-15 -0.26808445498721056 2313 16-17 0.29677301080684515 2313 18-19 0.20501075798428303 2313 20-21 0.5069291086310628 2313 22-23 -0.7402976455627197 2313 24-25 -0.687356731799035 2313 26-27 -0.15782143471697907 2313 28-29 -0.47847569392392586 2313 30-31 -0.6803362217959759 2313 32-33 -0.2557242339637895 2313 34-35 -0.09142333478665421 2313 36-37 -0.1821732404340466 2313 38-39 0.484848495991109 2313 40-41 1.6971509868827965 2313 42-43 1.7726893758114315 2313 44-45 0.5333497295943772 2313 46-47 -1.066299820989407 2313 48-49 0.08523424304964067 2313 50-51 0.07265501628263848 2313 52-53 -0.2856484781051911 2313 54-55 1.3097711687687017 2313 56-57 1.1290783336863761 2313 58-59 0.9236177139451733 2313 60-61 1.797069631462719 2313 62-63 1.9748365457295662 2313 64-65 1.0859937379310338 2313 66-67 0.5732902445170716 2313 68-69 1.3907349343844793 2313 70-71 1.6384803621569404 2313 72-73 1.0395344753917577 2313 74-75 0.871786602383569 2313 76-77 -0.1558858065394979 2313 78-79 -1.677397521351729 2313 80-81 -1.5497294654935523 2313 82-83 -1.2500115176106483 2313 84-85 -0.7256241559974512 2313 86-87 -0.6998440534589712 2313 88-89 -0.20319597776257936 2313 90-91 0.39752354998757156 2313 92-93 0.9687104937518143 2313 94-95 0.27933441945467763 2313 96-97 -0.579340356332164 2313 98-99 -0.3169482965146315 2313 100-101 -0.26302297670612873 2314 1 -0.3776955700135076 2314 2 -0.18152733698115497 2314 3 -0.09752080082616033 2314 4 0.03308021772964054 2314 5 0.0956759211633269 2314 6 0.34775219034028026 2314 7 0.26242527168465557 2314 8 0.01735056363134646 2314 9 0.2519542984984753 2314 10-11 -0.05003064055171791 2314 12-13 0.31113677507240567 2314 14-15 0.4806884142675827 2314 16-17 0.5641759559206321 2314 18-19 -0.11872864406837635 2314 20-21 -0.30632390341713034 2314 22-23 -0.3087938526131495 2314 24-25 -0.027048833539332406 2314 26-27 -0.10940554895169186 2314 28-29 0.12359927260887105 2314 30-31 -0.05249150961390825 2314 32-33 0.498961174336074 2314 34-35 0.6922669820362017 2314 36-37 0.7270190844209239 2314 38-39 -0.36834204394642 2314 40-41 0.07867709130046308 2314 42-43 0.13335425756065433 2314 44-45 0.13720961357474692 2314 46-47 0.04146456990751801 2314 48-49 0.17648814755655806 2314 50-51 0.7412181577373467 2314 52-53 0.7098892282758911 2314 54-55 1.069699772070802 2314 56-57 0.9852809218166776 2314 58-59 1.0573749651723041 2314 60-61 0.6838389308826187 2314 62-63 -0.016350424373065664 2314 64-65 1.0555385839819067 2314 66-67 0.6095463801707979 2314 68-69 0.415165991948065 2314 70-71 -0.23490205640620232 2314 72-73 0.6187401871275888 2314 74-75 0.849809806310386 2314 76-77 1.4290093295604542 2314 78-79 0.9091709748553178 2314 80-81 0.8106007442342467 2314 82-83 0.6037368090292468 2314 84-85 0.14530400107528507 2314 86-87 0.5968984721992179 2314 88-89 1.3721052270566965 2314 90-91 1.1170683960384409 2314 92-93 1.3890585526540917 2314 94-95 1.9442161686737762 2314 96-97 2.3446890948057195 2314 98-99 1.7494283210668051 2314 100-101 1.1385834489966804 2315 1 0.0810745755036244 2315 2 0.10966339999271923 2315 3 0.35702465371929293 2315 4 0.08033416859804987 2315 5 0.5639907388757379 2315 6 0.264510131194605 2315 7 0.20500076847702076 2315 8 -0.10610429476326999 2315 9 0.5012110658275333 2315 10-11 -0.14524265745048126 2315 12-13 -0.28477935698830237 2315 14-15 -0.2849194718222279 2315 16-17 0.09367055770439237 2315 18-19 -0.2334211804476638 2315 20-21 -0.35021374851179843 2315 22-23 -0.15756556283064072 2315 24-25 0.1196418251995155 2315 26-27 -0.14531542221096316 2315 28-29 0.1358821704339448 2315 30-31 -0.540365081824838 2315 32-33 0.010148781909222748 2315 34-35 -0.26470375806707125 2315 36-37 -1.713678771939577 2315 38-39 -0.48100046985785383 2315 40-41 0.3226921124239155 2315 42-43 0.5893079424300041 2315 44-45 0.3321712784159274 2315 46-47 0.8637146090250241 2315 48-49 0.6482453060607014 2315 50-51 0.4787396723672934 2315 52-53 0.7239715315148203 2315 54-55 0.02862638762391967 2315 56-57 0.03323898784703161 2315 58-59 0.18395922428668143 2315 60-61 -1.7997317153386305 2315 62-63 -2.216240895347873 2315 64-65 -1.3580899561526572 2315 66-67 -1.2288983076714786 2315 68-69 -1.327519033869489 2315 70-71 -1.3260458523692726 2315 72-73 -1.1961557602984776 2315 74-75 -1.0710945904976263 2315 76-77 -1.049945450599143 2315 78-79 -2.253636097590306 2315 80-81 -2.2161556319197224 2315 82-83 -1.9574429750421025 2315 84-85 -1.9116510920243854 2315 86-87 -1.6837305373454505 2315 88-89 -1.3882609128275156 2315 90-91 -1.8420146895506662 2315 92-93 -1.5933385682972485 2315 94-95 -0.7644366243163638 2315 96-97 -0.6265986535904595 2315 98-99 -1.891864121430455 2315 100-101 -1.856096569779723 2316 1 0.42953334564914414 2316 2 0.4690750726574038 2316 3 0.08320209074010165 2316 4 -0.03920893889686994 2316 5 0.34868796935609936 2316 6 0.03449917829208715 2316 7 0.4070035849376694 2316 8 0.5113264672458087 2316 9 0.2640024912695651 2316 10-11 0.269246467882013 2316 12-13 0.154510269048302 2316 14-15 0.5951462455928933 2316 16-17 0.9015253535109977 2316 18-19 0.7607894282207752 2316 20-21 0.8864471809202215 2316 22-23 0.4020495208808299 2316 24-25 0.43680658814741236 2316 26-27 0.7098715594820391 2316 28-29 0.4850450557414092 2316 30-31 0.22461692412102963 2316 32-33 0.908599728552943 2316 34-35 1.1862428856506568 2316 36-37 1.6728022169510481 2316 38-39 1.8063567512343113 2316 40-41 1.1509662479269664 2316 42-43 1.1152819684040267 2316 44-45 1.492631300321733 2316 46-47 1.6920669795460697 2316 48-49 2.0921507981589684 2316 50-51 2.35567598906265 2316 52-53 2.511094047552998 2316 54-55 2.111868446769595 2316 56-57 1.7202206808528189 2316 58-59 2.430868941075918 2316 60-61 2.7681762802802083 2316 62-63 2.8390712623739205 2316 64-65 2.308550632174679 2316 66-67 1.9348475849900737 2316 68-69 1.9995033413456582 2316 70-71 2.463893124316691 2316 72-73 2.2874148859227716 2316 74-75 1.9763158304067723 2316 76-77 2.02539487172913 2316 78-79 2.318809529072187 2316 80-81 2.063612792427019 2316 82-83 2.2422910258967157 2316 84-85 1.8862678564969713 2316 86-87 1.1993081107534351 2316 88-89 1.1612618535627242 2316 90-91 1.0989961068818133 2316 92-93 1.0456850586781847 2316 94-95 1.9261438795171522 2316 96-97 2.1820384923960816 2316 98-99 1.1108741041993362 2316 100-101 0.5181015212858355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 8.0 6 10.0 7 44.0 8 88.0 9 182.0 10 292.0 11 514.0 12 794.0 13 1113.0 14 1476.0 15 2097.0 16 2924.0 17 4171.0 18 3621.0 19 1990.0 20 1536.0 21 1472.0 22 1603.0 23 1706.0 24 1787.0 25 1825.0 26 2020.0 27 2206.0 28 2472.0 29 2849.0 30 3418.0 31 3947.0 32 4746.0 33 6004.0 34 8227.0 35 11998.0 36 14492.0 37 20132.0 38 17049.0 39 1664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.57640321084388 21.06598790183466 16.58015992885236 15.777448958469098 2 15.581459511656782 24.269247865605983 40.70599776214344 19.4432948605938 3 18.54508667862234 32.17072086571328 31.694026762312046 17.59016569335234 4 10.885792676384483 20.46949004460385 37.95849235909934 30.686224919912323 5 10.175363296339444 41.69553620700227 32.238334661843155 15.890765834815133 6 24.51679158811177 40.79106696709125 17.598595974923743 17.093545469873238 7 23.010836743765424 40.67304302509158 20.320044144019864 15.996076087123129 8 27.125580539844574 33.643720973007774 19.227173096672338 20.003525390475314 9 28.89747244830705 16.996214037185204 20.79290630125228 33.313407213255466 10-11 23.77760917214635 30.83682040434696 24.4010668138134 20.98450360969329 12-13 27.05910304999789 30.19992259083123 27.015799901130865 15.725174458040014 14-15 23.18633987829739 28.78098128477491 30.380435615640472 17.65224322128723 16-17 16.618384144939533 33.01413221747061 30.371622139452185 19.995861498137675 18-19 16.662451525880964 27.30453242592848 39.43762358026394 16.59539246792661 20-21 18.69913091460891 25.512714397388148 40.43737833570914 15.350776352293801 22-23 17.900170138409894 26.658466301865392 40.47952974356616 14.961833816158551 24-25 17.070170598243436 26.95544212994896 40.848162965006665 15.126224306800939 26-27 16.615126398197432 27.484566659385884 40.72064408091631 15.179662861500379 28-29 16.065803692548226 27.589113957035234 40.869411936020356 15.475670414396186 30-31 16.207661622528864 27.797810417568776 40.677797235623444 15.316730724278921 32-33 15.88648242669487 28.131083214543 40.2634079796447 15.719026379117427 34-35 16.239404668843214 28.154458086172806 39.987507855489646 15.618629389494338 36-37 16.380420287855795 28.37479499087997 39.609677963243975 15.635106758020264 38-39 16.145521987707117 28.631918578807802 39.48322373967291 15.739335693812173 40-41 15.992244140954309 28.938091077696544 39.246409466439815 15.823255314909337 42-43 16.142458025623434 29.002080993971635 38.80344762831838 16.05201335208655 44-45 16.382336260940207 29.36611946475376 37.90791066967091 16.343633604635123 46-47 16.822243681120767 29.888030532947074 36.737251115096335 16.552474670835824 48-49 16.90233135604911 30.311460584601708 35.68806425407336 17.09814380527582 50-51 17.144510353918548 30.68354255759415 34.661869070063304 17.510078018423997 52-53 17.13569687773026 31.241473919774375 33.979782652013306 17.643046550482058 54-55 17.013457794944898 31.24032433592373 33.82688799987738 17.919329869253996 56-57 17.255253598197452 31.36371300255974 33.36628807038519 18.01474532885762 58-59 17.214251774191077 30.54482610628286 33.89356386321485 18.347358256311217 60-61 17.459113134378686 30.473935102159682 34.179810242025724 17.887141521435908 62-63 17.39741880106068 30.394613816465103 34.503226498674145 17.704740883800064 64-65 17.239925813522174 30.273141122913504 34.40474548213547 18.082187581428858 66-67 17.36638003709324 30.387716313361228 34.00392391287687 18.24197973666866 68-69 17.39320366027498 30.297665578393957 33.78282061893594 18.52631014239512 70-71 17.183213010223632 30.347097683971736 33.707714474027064 18.761974831777565 72-73 17.34568752778161 30.062000889011507 33.51803313867047 19.07427844453641 74-75 16.886237182140064 30.364341441731423 33.04133903526923 19.708082340859274 76-77 16.6042059441149 30.086142149875077 34.03304670375991 19.276605202250117 78-79 16.722613080731442 29.899143176836652 33.46093714075505 19.91730660167686 80-81 16.262013151239252 30.246317499731763 33.225655645989484 20.2660137030395 82-83 16.38239903741143 30.09047259573196 32.86059709614007 20.666531270716536 84-85 16.178983913366697 29.91929859519777 32.67947057425985 21.22224691717568 86-87 16.088425989791695 29.606382489538785 32.51099768550451 21.794193835165004 88-89 16.151653101577228 29.20900967183213 32.336260940206316 22.30307628638433 90-91 16.11711504560435 29.240055185100022 32.687591017092046 21.95523875220357 92-93 15.553460710152592 29.00620023145487 32.56347764774408 22.87686141064846 94-95 15.105531797489308 28.838460477307216 32.70451096702994 23.35149675817354 96-97 15.603684799435937 29.05496543584556 32.10596097546022 23.235388789258288 98-99 15.758555810593839 29.53522146817748 31.167636791422538 23.538585929806143 100-101 15.480251105677473 28.926592213884394 31.19543472095537 24.397721959482766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1032.0 1 724.5 2 533.5 3 603.5 4 476.5 5 421.0 6 671.0 7 1371.5 8 2003.0 9 2073.0 10 1653.0 11 1150.5 12 999.5 13 993.0 14 997.0 15 961.5 16 939.5 17 940.5 18 948.0 19 987.0 20 959.5 21 975.0 22 1076.5 23 1198.5 24 1289.5 25 1369.5 26 1495.5 27 1703.5 28 1855.0 29 2039.0 30 2240.0 31 2373.5 32 2677.5 33 3044.0 34 3402.5 35 3655.5 36 3943.0 37 4257.0 38 4360.5 39 4230.0 40 4004.5 41 3989.5 42 3997.5 43 3968.0 44 3913.0 45 3833.0 46 3660.5 47 3486.5 48 3617.0 49 3430.5 50 2963.0 51 2544.5 52 2252.0 53 2115.5 54 1971.0 55 1873.5 56 1698.5 57 1553.5 58 1455.0 59 1353.5 60 1155.5 61 933.0 62 769.0 63 527.0 64 341.0 65 275.5 66 191.5 67 137.0 68 97.5 69 55.0 70 34.0 71 23.5 72 26.0 73 34.0 74 32.5 75 20.0 76 16.0 77 9.5 78 6.5 79 4.0 80 1.0 81 1.0 82 1.0 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03755307245443816 2 0.0 3 0.0 4 0.0 5 0.007663892337640441 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004981530019466287 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011495838506460662 28-29 0.0022991677012921324 30-31 3.8319461688202203E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011879033123342682 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.8319461688202203E-4 84-85 7.663892337640441E-4 86-87 0.0 88-89 0.0 90-91 0.00919667080516853 92-93 0.0022991677012921324 94-95 0.0 96-97 0.0 98-99 3.8319461688202203E-4 100-101 0.014561395441516838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 130482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.737251115096335 #Duplication Level Percentage of deduplicated Percentage of total 1 85.78380257212373 52.960561610030496 2 8.224092556730723 10.154657347373584 3 2.321366502805502 4.299443601416288 4 1.0328218878792392 2.550543369966739 5 0.6008242713143651 1.8546619457089866 6 0.4580664382541338 1.6967857635535937 7 0.29172252842742935 1.2607102895418527 8 0.18992998659317742 0.9380604221271899 9 0.16386116490391778 0.9104704097116844 >10 0.8664779780525348 9.24035499149308 >50 0.04220666368737276 1.7787894115663463 >100 0.014896469536719798 1.5703315399825262 >500 0.004965489845573266 2.0531567572538743 >1k 0.004965489845573266 8.731472540273755 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3912 2.9981146824849403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3176 2.434052206434604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 1.8922150181634247 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1836 1.4070906331907849 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 782 0.5993163808034825 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.5134807866219095 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 0.4805260495700557 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.4598335402584265 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.3180515320120783 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.26363789641483115 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.1233886666360111 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 155 0.11879033123342683 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 147 0.11265921736331448 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.10116337885685382 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.663892337640441E-4 0.0 10-11 0.0 0.0 0.0 0.0015327784675280881 0.0 12-13 0.0 0.0 0.0 0.0022991677012921324 0.0 14-15 0.0 0.0 0.0 0.0022991677012921324 0.0 16-17 0.0 0.0 0.0 0.0026823623181741543 0.0 18-19 0.0 0.0 0.0 0.003448751551938198 0.0 20-21 0.0 0.0 0.0 0.0038319461688202205 0.0 22-23 0.0 0.0 0.0 0.004598335402584265 0.0 24-25 0.0 0.0 0.0 0.0061311138701123525 0.0 26-27 0.0 0.0 0.0 0.00919667080516853 0.0 28-29 0.0 0.0 0.0 0.014561395441516838 0.0 30-31 0.0 0.0 0.0 0.026823623181741543 0.0 32-33 0.0 0.0 0.0 0.039085850921966245 0.0 34-35 0.0 0.0 0.0 0.04713293787648871 0.0 36-37 0.0 0.0 0.0 0.052114467895955 0.0 38-39 0.0 0.0 0.0 0.06705905795435385 0.0 40-41 0.0 0.0 0.0 0.10039698962308977 0.0 42-43 0.0 0.0 0.0 0.16285771217485936 0.0 44-45 0.0 0.0 0.0 0.21343940160328628 0.0 46-47 0.0 0.0 0.0 0.348323906745758 0.0 48-49 0.0 0.0 0.0 0.49623702886221854 0.0 50-51 0.0 0.0 0.0 0.6180929170307016 0.0 52-53 0.0 0.0 0.0 0.7617908983614599 0.0 54-55 0.0 0.0 0.0 0.8847963703805889 0.0 56-57 0.0 0.0 0.0 1.1576309376005884 0.0 58-59 0.0 0.0 0.0 1.5017397035606446 0.0 60-61 0.0 0.0 0.0 1.8604098649622172 0.0 62-63 0.0 0.0 0.0 2.2209959994481996 0.0 64-65 0.0 0.0 0.0 2.644042856485952 0.0 66-67 0.0 0.0 0.0 3.1490933615364574 0.0 68-69 0.0 0.0 0.0 3.7441946015542378 0.0 70-71 0.0 0.0 0.0 4.3281831976824385 0.0 72-73 0.0 0.0 0.0 4.823653837310893 0.0 74-75 0.0 0.0 0.0 5.347480878588618 0.0 76-77 0.0 0.0 0.0 5.944114897073926 0.0 78-79 0.0 0.0 0.0 6.532318633987829 0.0 80-81 0.0 0.0 0.0 7.214405052037829 0.0 82-83 0.0 0.0 0.0 7.853190478380159 0.0 84-85 0.0 0.0 0.0 8.653300838429821 0.0 86-87 0.0 0.0 0.0 9.526218175687069 0.0 88-89 0.0 0.0 0.0 10.856286690884566 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 15 6.604668E-4 95.0 3 TATAACG 20 0.002069945 71.25 2 TAACGCA 25 0.0050113294 57.0 4 GGTATCA 1155 0.0 55.51948 1 GTATCAA 2140 0.0 42.39486 1 GGATTTA 45 9.5936796E-4 42.22222 1 GTCGGGA 45 9.5936796E-4 42.22222 2 CTGATAT 70 4.2808206E-6 40.714287 4 GTGGGTA 50 0.001610569 38.0 9 TACTGAT 75 6.8752506E-6 38.0 2 TCGGGAG 55 0.002571286 34.545456 3 CGGGAGT 55 0.002571286 34.545456 4 GAGCAGA 70 2.0434044E-4 33.928574 6 ACACAGT 60 0.0039382363 31.666668 6 GGGAGTG 75 3.063619E-4 31.666668 5 GATATTA 75 3.063619E-4 31.666668 6 TATCAAC 2930 0.0 30.80205 2 ATCAACG 2930 0.0 30.639933 3 TCAACGC 3005 0.0 29.87521 4 GCTTACA 65 0.0058253477 29.23077 2 >>END_MODULE