FastQCFastQC Report
Thu 26 May 2016
SRR937883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825540
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44790.5425539646776655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41510.5028223950383991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.310463454223902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10740.1300966640017443No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.1270683431450929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.047.8817671
GTATCAA37150.039.427961
ATCAACG47850.030.2983683
TATCAAC48350.029.9850462
TCAACGC48300.029.8228844
CAACGCA48700.029.7731675
AACGCAG50650.028.7918996
CGTTCGC350.00838224627.14973346-47
TACCTGG6200.026.066852
ACGCAGA56500.025.73147
CGCAGAG57050.025.2304338
TGGTATC11550.024.6928182
GGACCGA1000.00165285623.750976
GCAGAGT59700.023.473919
GAGTACT37650.023.21546612-13
GTGGTAT12750.023.1256481
GTACATG31050.022.361641
GTACCTG7250.022.3024881
TACATGG31200.022.090972
GGGCGCG1302.9360995E-421.9279657
Produced by FastQC (version 0.11.5)