##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 825540 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54922596118904 33.0 31.0 34.0 30.0 34.0 2 31.681922135814133 33.0 31.0 34.0 30.0 34.0 3 31.673463429997334 33.0 31.0 34.0 30.0 34.0 4 35.27944254669671 37.0 35.0 37.0 33.0 37.0 5 35.2624197494973 37.0 35.0 37.0 33.0 37.0 6 35.32517867093054 37.0 35.0 37.0 33.0 37.0 7 35.259741502531675 37.0 35.0 37.0 33.0 37.0 8 35.2548441020423 37.0 35.0 37.0 33.0 37.0 9 36.86394602320905 39.0 37.0 39.0 33.0 39.0 10-11 36.72020011144221 39.0 37.0 39.0 32.0 39.0 12-13 36.595693727741846 39.0 37.0 39.0 32.0 39.0 14-15 37.70636916442571 40.0 37.5 41.0 32.0 41.0 16-17 37.60752234900792 40.0 37.0 41.0 32.0 41.0 18-19 37.54724119969959 40.0 37.0 41.0 32.0 41.0 20-21 37.46358020204957 40.0 37.0 41.0 32.0 41.0 22-23 37.37649659616736 40.0 37.0 41.0 32.0 41.0 24-25 37.39394396395087 40.0 37.0 41.0 32.0 41.0 26-27 37.289816362623256 40.0 37.0 41.0 31.5 41.0 28-29 37.14926714635269 40.0 37.0 41.0 31.0 41.0 30-31 36.88052184025001 39.0 36.0 41.0 30.5 41.0 32-33 36.714276715846594 39.0 36.0 41.0 30.0 41.0 34-35 36.70224580274729 39.0 36.0 41.0 30.0 41.0 36-37 36.55807774305303 39.0 35.5 41.0 30.0 41.0 38-39 36.38798362284081 39.0 35.0 41.0 30.0 41.0 40-41 36.14633088645008 39.0 35.0 41.0 29.5 41.0 42-43 35.86692770792451 39.0 35.0 41.0 28.5 41.0 44-45 35.60609116457107 39.0 35.0 40.0 27.0 41.0 46-47 35.23732344889406 39.0 35.0 40.0 25.5 41.0 48-49 34.87717433437507 38.5 34.5 40.0 24.0 41.0 50-51 33.97366996147976 37.5 33.5 39.5 23.5 40.5 52-53 34.051858783341814 38.0 33.5 39.5 23.0 40.5 54-55 34.32148714780628 38.0 34.0 40.0 23.0 41.0 56-57 34.08808779707827 38.0 34.0 40.0 22.0 41.0 58-59 33.829365021682776 38.0 33.5 40.0 20.5 41.0 60-61 33.49858819681663 38.0 33.0 40.0 18.5 41.0 62-63 33.109737262882476 37.0 32.5 40.0 17.5 41.0 64-65 32.710941323255085 36.5 32.0 39.5 13.5 41.0 66-67 32.25222097051626 36.0 31.5 39.0 9.5 40.5 68-69 31.744731327373596 35.5 31.0 39.0 7.5 40.0 70-71 31.282667708409043 35.0 30.5 38.5 4.5 40.0 72-73 30.807828209414446 35.0 30.0 37.5 2.0 40.0 74-75 30.266586718996052 35.0 29.5 37.0 2.0 39.0 76-77 29.76070814254912 34.0 29.0 36.5 2.0 39.0 78-79 29.177078639436004 34.0 28.5 36.0 2.0 38.5 80-81 28.59654286891005 34.0 27.5 35.5 2.0 37.0 82-83 28.083267921602832 34.0 26.5 35.0 2.0 37.0 84-85 27.55199021246699 33.0 26.0 35.0 2.0 36.5 86-87 26.989240375996317 33.0 25.5 35.0 2.0 36.0 88-89 26.51971134045594 32.5 25.0 35.0 2.0 35.5 90-91 25.94906546018364 32.0 23.5 35.0 2.0 35.0 92-93 25.295923274462776 32.0 20.0 34.0 2.0 35.0 94-95 24.52529193013058 31.0 15.0 34.0 2.0 35.0 96-97 23.68001247668193 31.0 3.5 34.0 2.0 35.0 98-99 22.613055091213027 31.0 2.0 34.0 2.0 35.0 100-101 19.892082152288197 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04884756147844982 1101 2 -0.014562634207077707 1101 3 0.013378317264937323 1101 4 -0.10256405004607672 1101 5 -0.16708991564556186 1101 6 -0.10687632425126026 1101 7 -0.04867071176640536 1101 8 -0.14766667365658037 1101 9 -0.14951620478110783 1101 10-11 -0.07607149869196661 1101 12-13 -0.059464956260598 1101 14-15 0.13683387350754828 1101 16-17 0.24575297681059993 1101 18-19 0.09440739221354733 1101 20-21 0.023576932754856728 1101 22-23 0.02468086999478203 1101 24-25 0.03606520692708415 1101 26-27 -0.02124627277815705 1101 28-29 0.053774297023650774 1101 30-31 0.21866539082997605 1101 32-33 0.1231419476284259 1101 34-35 0.07854577157204545 1101 36-37 0.010012530396473096 1101 38-39 -0.14378945103464247 1101 40-41 -0.12429160712659382 1101 42-43 -0.13912586297801255 1101 44-45 -0.14419102629551617 1101 46-47 -0.03682470925645731 1101 48-49 0.20407621343788662 1101 50-51 0.1729443598666336 1101 52-53 0.20946822646843088 1101 54-55 0.15925112008056175 1101 56-57 -0.03620346144442976 1101 58-59 -0.0740116327308229 1101 60-61 -0.05937974897008047 1101 62-63 0.027729187708551706 1101 64-65 0.0671031600356784 1101 66-67 0.14127905255126194 1101 68-69 -0.18269654463044915 1101 70-71 -0.04093667411546065 1101 72-73 -0.031421333892847514 1101 74-75 0.21433112554274558 1101 76-77 0.07874876528028452 1101 78-79 0.012327014340467457 1101 80-81 0.1982146045882942 1101 82-83 0.04456033148381877 1101 84-85 0.03602339867834914 1101 86-87 -0.1972987886873412 1101 88-89 -0.04903308401780038 1101 90-91 -0.1551526155934546 1101 92-93 -0.3686920958624711 1101 94-95 -0.3146441545762997 1101 96-97 0.04532126654681079 1101 98-99 0.15007872581239923 1101 100-101 0.32402247856458644 1102 1 0.11405537499006257 1102 2 0.24727362455540813 1102 3 0.38350720173808384 1102 4 0.3558141306681648 1102 5 0.15661502998561616 1102 6 0.22493219813102883 1102 7 0.11957941242634007 1102 8 0.13470623576551333 1102 9 0.2496185857471218 1102 10-11 0.028117591665157704 1102 12-13 0.22569711527442848 1102 14-15 0.23012677520208769 1102 16-17 0.3909756958038102 1102 18-19 0.3312550353111945 1102 20-21 0.2888043434740766 1102 22-23 0.3980042679083482 1102 24-25 0.5572935421923049 1102 26-27 0.20661981984203237 1102 28-29 0.3560956664556727 1102 30-31 0.3122032282202767 1102 32-33 0.39331981924072323 1102 34-35 0.2680380302241403 1102 36-37 0.33606506392851543 1102 38-39 0.32467594058648785 1102 40-41 0.49053148720469153 1102 42-43 0.42619434732170447 1102 44-45 0.24606570771121739 1102 46-47 0.3188322703759354 1102 48-49 0.450196914988922 1102 50-51 0.47581769706373933 1102 52-53 0.5237189992232985 1102 54-55 0.42038534484184 1102 56-57 0.3700879361379563 1102 58-59 0.39771036870429555 1102 60-61 0.4565151977650288 1102 62-63 0.4951475654752926 1102 64-65 0.3571518711909434 1102 66-67 0.5434738143771725 1102 68-69 0.4340567119793626 1102 70-71 0.5494066498344203 1102 72-73 0.4325041228604789 1102 74-75 0.26748389642092363 1102 76-77 0.30768084564678944 1102 78-79 0.5172498768453018 1102 80-81 0.31084190215411667 1102 82-83 0.4158673221555631 1102 84-85 0.511421160449057 1102 86-87 0.7204769882072881 1102 88-89 0.6484109602500148 1102 90-91 0.9359410395370844 1102 92-93 0.6956397471578981 1102 94-95 0.6225850362389593 1102 96-97 0.610570755976628 1102 98-99 0.5012751159432192 1102 100-101 0.6755621236493106 1103 1 0.07543880862959185 1103 2 0.010797803628371128 1103 3 0.07344402643100523 1103 4 -0.03555381710221894 1103 5 -0.04331677939741496 1103 6 -0.10036168143113855 1103 7 0.004354427384285486 1103 8 -0.004974544159175309 1103 9 -0.143170806432515 1103 10-11 0.03776656396656364 1103 12-13 0.029592119321868893 1103 14-15 0.07171029330999801 1103 16-17 0.03102426512067069 1103 18-19 -0.03227992227864718 1103 20-21 0.05372445046734953 1103 22-23 -0.05592879090276881 1103 24-25 -0.01157436158413816 1103 26-27 -0.08459722869208974 1103 28-29 -0.28387921300091534 1103 30-31 -0.06992203134400654 1103 32-33 -0.004009308271328393 1103 34-35 -0.11033740531593139 1103 36-37 -0.026013694034382695 1103 38-39 0.08133002498447439 1103 40-41 -0.10987853637605838 1103 42-43 -0.05385901733531995 1103 44-45 -0.05926393892940496 1103 46-47 -0.19887925251851613 1103 48-49 -0.10063259087107213 1103 50-51 -0.37428986813528553 1103 52-53 -0.2155035837492818 1103 54-55 -0.1264635887449046 1103 56-57 -0.27317153135931704 1103 58-59 -0.1252364338156795 1103 60-61 -0.04895830908042598 1103 62-63 -0.0021659441857835304 1103 64-65 -0.24227488768703864 1103 66-67 -0.11293566367864827 1103 68-69 -0.07020080218668312 1103 70-71 -0.13801636075932322 1103 72-73 -0.23354090934309113 1103 74-75 -0.3794178299015307 1103 76-77 -0.3076875596606392 1103 78-79 -0.19209047792188372 1103 80-81 -0.29375876479410934 1103 82-83 -0.4784211637487772 1103 84-85 -0.428642586779322 1103 86-87 -0.4002527393821964 1103 88-89 -0.34419607702166033 1103 90-91 -0.4511210007261752 1103 92-93 -0.7135884230458807 1103 94-95 -0.5662169646057293 1103 96-97 -0.483158346898815 1103 98-99 -0.36219319081777357 1103 100-101 -0.15200954248942722 1104 1 0.18112103972145022 1104 2 0.2620530885567831 1104 3 0.2103538819598647 1104 4 0.24143417508975062 1104 5 0.218840457257528 1104 6 0.12263089079738876 1104 7 0.046238382890891216 1104 8 0.2139771175828784 1104 9 0.23999629166646486 1104 10-11 0.11110770908837964 1104 12-13 0.42256601649356895 1104 14-15 0.25259835845580625 1104 16-17 0.11690107734762734 1104 18-19 0.07247559784844526 1104 20-21 0.3129840957257741 1104 22-23 0.19922177850337874 1104 24-25 0.19875438138454626 1104 26-27 -0.0018656002440309294 1104 28-29 0.24196757266408042 1104 30-31 0.14922849299412633 1104 32-33 0.21402127600725152 1104 34-35 0.06874033418534253 1104 36-37 -0.0186402858506014 1104 38-39 0.27610974099967933 1104 40-41 0.36394427310737854 1104 42-43 0.2295984877559576 1104 44-45 0.18445653800282003 1104 46-47 0.19208123128657206 1104 48-49 1.2726703474186252E-5 1104 50-51 -0.04497200064086826 1104 52-53 -0.024257481427994776 1104 54-55 0.16600723676021545 1104 56-57 0.13068629033473655 1104 58-59 0.12745680232098522 1104 60-61 0.3204667076088157 1104 62-63 0.35482227334624383 1104 64-65 0.37873441040687084 1104 66-67 0.38900930264710354 1104 68-69 0.15228869837526204 1104 70-71 0.13473242170020683 1104 72-73 0.1995618260034675 1104 74-75 0.32589822360545995 1104 76-77 0.3192583221090679 1104 78-79 0.039660217111340046 1104 80-81 0.04282293928369896 1104 82-83 0.17395828636516342 1104 84-85 0.28987388698687155 1104 86-87 0.2290180883763142 1104 88-89 0.3751057068265311 1104 90-91 0.5478072256504447 1104 92-93 0.5887339878673359 1104 94-95 0.5356865954854229 1104 96-97 0.7914508993668754 1104 98-99 0.8846000794015687 1104 100-101 0.7873221552506955 1105 1 0.09754051825147059 1105 2 0.15248085247664633 1105 3 0.08631838752592458 1105 4 0.2586238425732077 1105 5 0.22087877639916798 1105 6 0.1379190707482536 1105 7 0.1350822029777703 1105 8 0.23741621493463327 1105 9 0.17822861601084838 1105 10-11 0.3621964640465549 1105 12-13 0.30834386487155996 1105 14-15 0.26991424130277863 1105 16-17 0.48416930504800604 1105 18-19 0.2936796216414166 1105 20-21 0.36838910156077986 1105 22-23 0.41691418419179627 1105 24-25 0.4152542288251695 1105 26-27 0.3361249882577084 1105 28-29 0.3180707185328657 1105 30-31 0.39885771078915155 1105 32-33 0.2860383469300629 1105 34-35 0.22657402244204405 1105 36-37 0.4037297948957814 1105 38-39 0.5268214226444243 1105 40-41 0.6872407982136224 1105 42-43 0.6327799013051205 1105 44-45 0.7694132394488307 1105 46-47 0.47507612919761044 1105 48-49 0.3643174491240444 1105 50-51 0.462180596485986 1105 52-53 0.38399613831852974 1105 54-55 0.5965475014271462 1105 56-57 0.6863598478621995 1105 58-59 0.5047904103660485 1105 60-61 0.6969730348911511 1105 62-63 0.7055185417752554 1105 64-65 0.4441969327009829 1105 66-67 0.5804559902212176 1105 68-69 0.7420085553154543 1105 70-71 0.776890633641365 1105 72-73 0.5063088039122405 1105 74-75 0.5416592589960949 1105 76-77 0.4497803425614606 1105 78-79 0.1672100820097846 1105 80-81 0.46356440932701304 1105 82-83 0.723116614691822 1105 84-85 0.7513066397557431 1105 86-87 0.5235257147725498 1105 88-89 0.27922174317655646 1105 90-91 0.4765403694117083 1105 92-93 0.6264999051581341 1105 94-95 0.7889237639833269 1105 96-97 0.7979209112256136 1105 98-99 0.99237777679296 1105 100-101 1.2569978328817157 1106 1 0.1163322546268688 1106 2 0.0536927180481932 1106 3 0.09262179468337095 1106 4 0.13838635104489327 1106 5 -0.15472845972267635 1106 6 -0.039885589981516034 1106 7 0.13281084366934692 1106 8 0.1741475428682051 1106 9 0.12779898904933162 1106 10-11 -0.06779009974344774 1106 12-13 -0.1880056092915794 1106 14-15 0.0908957007548139 1106 16-17 -0.07399680631851169 1106 18-19 -0.010117351775271288 1106 20-21 -0.028096393493655114 1106 22-23 0.050682081365692966 1106 24-25 0.11663505414495745 1106 26-27 0.04492061094428834 1106 28-29 0.18376371416787407 1106 30-31 -0.16537246783897785 1106 32-33 0.043156339129005516 1106 34-35 0.2807892482509331 1106 36-37 0.1699977987062553 1106 38-39 0.18992326769435408 1106 40-41 0.04336493741938341 1106 42-43 0.17223306888569567 1106 44-45 0.0172200803043836 1106 46-47 0.19650285564396341 1106 48-49 0.03820973195270483 1106 50-51 -0.21741141193120228 1106 52-53 -0.0127891240311655 1106 54-55 -0.12717191164507824 1106 56-57 0.014444488876051764 1106 58-59 -0.3464432564633384 1106 60-61 -0.2562999974268436 1106 62-63 -0.08600693015088723 1106 64-65 0.1331587425845342 1106 66-67 0.020968770023770844 1106 68-69 -0.2068426131845733 1106 70-71 -0.43297194734320144 1106 72-73 -0.03477294132584419 1106 74-75 -0.24731582121831863 1106 76-77 0.008868267982201417 1106 78-79 -0.03441245290777317 1106 80-81 -0.187169961303848 1106 82-83 -0.26458403981985157 1106 84-85 0.08393873879032654 1106 86-87 0.2246535744103646 1106 88-89 0.3305394159396755 1106 90-91 0.23407424430092405 1106 92-93 0.18292428559956164 1106 94-95 0.41311095621828997 1106 96-97 0.46282195247264823 1106 98-99 0.3272095516523166 1106 100-101 0.20891598811614998 1107 1 0.19174439389195186 1107 2 0.26023117030998577 1107 3 0.21531538255616667 1107 4 0.26561136039114075 1107 5 0.12241786026864077 1107 6 0.166138818793101 1107 7 0.2428854930344002 1107 8 0.1497327384157785 1107 9 0.4388375076393132 1107 10-11 0.3532032252285191 1107 12-13 0.31095876643954057 1107 14-15 0.30684069659422875 1107 16-17 0.5060372019544275 1107 18-19 0.6750362884364947 1107 20-21 0.46314677771749757 1107 22-23 0.4166998874703012 1107 24-25 0.39722441027910804 1107 26-27 0.36959861772182734 1107 28-29 0.44817049893892147 1107 30-31 0.5246932272201761 1107 32-33 0.6480890461634132 1107 34-35 0.7794860500261223 1107 36-37 0.5902811105331764 1107 38-39 0.6024537183642664 1107 40-41 0.7304179758535554 1107 42-43 0.7396850713941134 1107 44-45 0.7899857247129205 1107 46-47 0.8124128784367386 1107 48-49 0.8355198222796503 1107 50-51 0.90179466976101 1107 52-53 0.8843019325166281 1107 54-55 0.8864163229396453 1107 56-57 1.0405393562799716 1107 58-59 1.163449442611686 1107 60-61 1.3718536066059102 1107 62-63 1.010477223441086 1107 64-65 1.342519157919618 1107 66-67 1.2004332603217662 1107 68-69 1.5896941507232434 1107 70-71 1.1149569548649225 1107 72-73 0.9842217946276541 1107 74-75 0.8757478478160614 1107 76-77 0.9112912066828933 1107 78-79 0.901419543811997 1107 80-81 1.0781842613900388 1107 82-83 1.065591669590308 1107 84-85 0.9878781811511281 1107 86-87 0.9304511106195292 1107 88-89 1.3337638705410448 1107 90-91 1.282028773310465 1107 92-93 1.1031367468578672 1107 94-95 1.2420626089962319 1107 96-97 1.5790613550365329 1107 98-99 1.5402165100826295 1107 100-101 1.3022216646034082 1108 1 0.05036788323653241 1108 2 0.04817358937046734 1108 3 0.1126069803661629 1108 4 0.12346066128639421 1108 5 0.07964888816098181 1108 6 0.07362778975114992 1108 7 -0.07740219611186916 1108 8 0.15170862540941243 1108 9 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62-63 0.5898687210387905 2316 64-65 0.7076522085527728 2316 66-67 0.2924332019662259 2316 68-69 0.5022751190361809 2316 70-71 0.43822112067246977 2316 72-73 0.323470515668717 2316 74-75 0.6150627051367685 2316 76-77 0.5418459247283707 2316 78-79 0.8155955635750765 2316 80-81 0.7779559453364335 2316 82-83 0.6160983902034332 2316 84-85 0.3416866096364153 2316 86-87 0.4217573507720296 2316 88-89 0.5830514466463939 2316 90-91 0.5404283239727974 2316 92-93 0.6569125934995057 2316 94-95 0.7992218612672346 2316 96-97 0.4817109397396031 2316 98-99 -0.12145265290439511 2316 100-101 -0.36891568488792714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4038.0 3 931.0 4 885.0 5 940.0 6 1058.0 7 1225.0 8 1632.0 9 1807.0 10 2351.0 11 3004.0 12 3815.0 13 4729.0 14 5362.0 15 6483.0 16 7888.0 17 9959.0 18 9000.0 19 6680.0 20 6667.0 21 6982.0 22 7641.0 23 8084.0 24 9232.0 25 10396.0 26 11876.0 27 13627.0 28 16017.0 29 19134.0 30 23052.0 31 27961.0 32 34694.0 33 44360.0 34 59177.0 35 80380.0 36 111804.0 37 147132.0 38 105110.0 39 10422.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.442768390145254 19.041554047484784 15.118639041748496 22.39703852062146 2 14.998153475353757 20.034306484217073 41.56441941377702 23.403120626652154 3 18.797786194815828 24.714831668687243 30.63953022237663 25.847851914120294 4 12.379228446992936 17.066009026356234 37.78665970151067 32.76810282514017 5 13.284370007229077 37.15113630150717 34.823130615443496 14.741363075820257 6 30.83646064442837 36.470111320947076 17.972045965404575 14.721382069219976 7 27.366821533815898 33.290247253414115 21.26583261725482 18.077098595515164 8 25.567050850090787 35.51714260746293 20.129455572925263 18.786350969521017 9 25.729335445150408 17.05073822570955 21.0901345373118 36.12979179182824 10-11 24.424355231128786 27.021637239866703 27.609101077158645 20.944906451845867 12-13 25.328849359474205 24.52851828006187 28.362510111090007 21.780122249373917 14-15 22.331059165777166 25.981277105991822 26.87342719255502 24.81423653567599 16-17 21.263037538643793 29.230122191459586 27.6435267171681 21.86331355272852 18-19 21.170111669804005 27.280092044755307 30.52811649292976 21.021679792510923 20-21 21.841206930237693 26.434182279765206 30.809025279081986 20.915585510915115 22-23 21.55917599587391 26.31655592973514 30.823882770547012 21.300385303843935 24-25 21.341299502599867 26.565669621212535 30.85531341912378 21.237717457063816 26-27 21.22720046600245 26.746659708991178 30.64136014465493 21.384779680351443 28-29 21.06412379680264 26.885836058689268 30.916975816802676 21.133064327705416 30-31 21.195346742683462 26.5974026919851 30.857628727455022 21.349621837876416 32-33 21.03606911578168 26.75377957964884 30.69540624032725 21.514745064242234 34-35 21.44138578120126 26.77176632239004 30.543130874075835 21.243717022332866 36-37 21.388500915563117 26.602114107438208 30.500083122695166 21.509301854303512 38-39 21.20306882531162 26.897419065981197 30.57092138774193 21.328590720965252 40-41 21.435332091567112 26.87184123189681 30.409843525321715 21.282983151214363 42-43 21.2915913732905 26.785408720156212 30.291056178466235 21.631943728087048 44-45 21.31337055721123 27.064016695522152 30.17221656844879 21.45039617881783 46-47 21.442260311048862 27.427535616235517 29.559836974859273 21.570367097856348 48-49 21.545418023504908 27.46843416719969 29.363173053465598 21.6229747558298 50-51 21.5381179499286 27.621380935532752 29.249280996645766 21.59122011789288 52-53 21.646101426586434 27.799162174122337 28.935344390627666 21.619392008663564 54-55 21.624137307513543 27.574728252625242 29.05624748943896 21.74488695042226 56-57 21.501108707983608 27.9305836566433 28.979776317026783 21.58853131834631 58-59 21.63756597706728 27.585087666080206 29.063034641752104 21.714311715100408 60-61 21.556626865617318 27.441127993647363 29.43676146486788 21.565483675867437 62-63 21.455945903150088 27.623379924686176 29.418680910002593 21.501993262161143 64-65 21.421616476467864 27.51912048499026 29.39965286772392 21.659610170817956 66-67 21.286334365644365 27.80742596631289 29.0998268022725 21.806412865770245 68-69 21.255981492638373 27.685323887929265 29.254785042430903 21.80390957700146 70-71 21.360658124635993 27.711245389244805 29.032226752086977 21.895869734032228 72-73 21.43342908536086 27.68523770668333 28.977495745758652 21.90383746219716 74-75 21.370550645786178 27.932337600960018 28.677165153694624 22.01994659955918 76-77 21.45948422052136 27.83134174865079 28.727539687595748 21.981634343232102 78-79 21.52553325101857 27.798464334145358 28.646083930722117 22.02991848411396 80-81 21.413594904792472 28.004773420275754 28.685841239487615 21.895790435444162 82-83 21.49913884782146 27.91091579393742 28.650372215511904 21.939573142729223 84-85 21.523140843301793 27.83022101381359 28.591178738317048 22.055459404567564 86-87 21.536125575684633 27.747271412556596 28.676616891278712 22.039986120480055 88-89 21.50679446736229 27.646748362048047 28.599369085173503 22.247088085416163 90-91 21.45758002281166 27.711614531511636 28.770475890018687 22.060329555658022 92-93 21.58370530162493 27.588359404478663 28.713849110491264 22.11408618340514 94-95 21.386862775193695 27.676770373084665 28.629487016930376 22.306879834791264 96-97 21.372624079312725 27.626169267533022 28.598529084344992 22.40267756880926 98-99 21.509826143511688 27.665241321736428 28.38157422148016 22.443358313271723 100-101 21.795886579798278 27.122807592158914 28.816868552878905 22.2644372751639 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1304.0 1 793.5 2 348.5 3 421.5 4 343.0 5 287.5 6 478.0 7 998.5 8 1615.5 9 1954.0 10 1724.0 11 1366.0 12 1376.0 13 1458.5 14 1587.0 15 1646.5 16 1691.5 17 1781.0 18 1845.0 19 1990.0 20 2159.5 21 2441.0 22 2861.5 23 3415.0 24 4101.5 25 4953.5 26 6082.0 27 7485.0 28 8673.0 29 9834.0 30 11443.5 31 12861.0 32 14128.5 33 15357.0 34 16772.5 35 18313.5 36 20178.5 37 22154.5 38 23402.0 39 24163.0 40 25029.0 41 26428.0 42 27662.0 43 28981.0 44 30202.0 45 30931.5 46 31051.5 47 31206.5 48 31972.0 49 31903.0 50 30660.0 51 28780.0 52 27190.0 53 26181.5 54 24863.5 55 22611.0 56 19930.0 57 17456.5 58 15416.5 59 13640.0 60 11488.5 61 9123.5 62 7047.0 63 5302.5 64 3908.5 65 2920.0 66 2181.0 67 1649.0 68 1215.0 69 868.5 70 664.5 71 494.5 72 386.5 73 295.0 74 203.0 75 164.0 76 121.0 77 74.5 78 52.0 79 33.5 80 24.5 81 21.5 82 14.0 83 10.0 84 8.5 85 6.5 86 7.0 87 4.5 88 2.0 89 2.5 90 2.5 91 3.0 92 3.0 93 2.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3280277151924801 2 0.2873270828790852 3 0.2836930978511035 4 0.2901131380672045 5 0.29980376480848897 6 0.19575066017394677 7 0.1947815974998183 8 0.19720425418513943 9 0.19441819899702015 10-11 0.20101993846452018 12-13 0.18999685054630908 14-15 0.19084478038617148 16-17 0.20217070039004773 18-19 0.19296460498582746 20-21 0.18157811856481818 22-23 0.18351624391307508 24-25 0.18842212370085035 26-27 0.1829105797417448 28-29 0.19883954744773116 30-31 0.20435109140683674 32-33 0.20653148242362573 34-35 0.20435109140683674 36-37 0.17649053952564384 38-39 0.16728444412142354 40-41 0.17503694551445115 42-43 0.18666569760399254 44-45 0.19459989824841922 46-47 0.19599292584247885 48-49 0.19708312135087339 50-51 0.200656539961722 52-53 0.2253676381519975 54-55 0.1842430409186714 56-57 0.16746614337282265 58-59 0.16837463962981805 60-61 0.1593502434769969 62-63 0.16819294037841898 64-65 0.16455895535043727 66-67 0.1620151658308501 68-69 0.1738861835889236 70-71 0.1671633112871575 72-73 0.16504348668750152 74-75 0.16831407321268504 76-77 0.1753397776001163 78-79 0.1777624342854374 80-81 0.16873803813261623 82-83 0.163953291179107 84-85 0.1593502434769969 86-87 0.1579572158829372 88-89 0.16231799791651527 90-91 0.16958596797247863 92-93 0.16001647406546018 94-95 0.16601254936162996 96-97 0.16468008818470334 98-99 0.1710395619836713 100-101 0.14408750635947382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 825540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.06421126600364 #Duplication Level Percentage of deduplicated Percentage of total 1 84.85954739982178 57.758981621589186 2 8.949928547770721 12.183396549822067 3 2.550546285639712 5.208027636885066 4 1.0810119696108644 2.9431290832269035 5 0.6439357889968519 2.1914490792011234 6 0.3841025925206604 1.5686184003087564 7 0.2903265547397599 1.383259357055659 8 0.20699431100536697 1.1271123612104126 9 0.15524544409700888 0.9509992834593032 >10 0.8283174456550909 9.913187688240818 >50 0.03668778881525356 1.68843935264751 >100 0.012109323356186644 1.328556275350373 >500 3.5615656310089024E-4 0.14084320537264866 >1k 8.903914077522256E-4 1.6140001056301114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4479 0.5425539646776655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4151 0.5028223950383991 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2563 0.310463454223902 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1074 0.1300966640017443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 0.1270683431450929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.2113283426605616E-4 0.0 0.0 0.0 0.0 3 1.2113283426605616E-4 0.0 0.0 0.0 0.0 4 1.2113283426605616E-4 0.0 0.0 0.0 0.0 5 1.2113283426605616E-4 0.0 0.0 0.0 0.0 6 1.2113283426605616E-4 0.0 0.0 0.0 0.0 7 1.2113283426605616E-4 0.0 0.0 0.0 0.0 8 1.2113283426605616E-4 0.0 0.0 0.0 0.0 9 1.2113283426605616E-4 0.0 0.0 0.0 0.0 10-11 1.2113283426605616E-4 0.0 0.0 0.0 0.0 12-13 1.8169925139908423E-4 0.0 0.0 0.0 0.0 14-15 2.4226566853211232E-4 0.0 0.0 0.0 0.0 16-17 3.028320856651404E-4 0.0 0.0 0.0 0.0 18-19 4.239649199311965E-4 0.0 0.0 0.0 0.0 20-21 4.8453133706422464E-4 0.0 0.0 6.056641713302808E-5 0.0 22-23 4.8453133706422464E-4 0.0 0.0 2.4226566853211232E-4 0.0 24-25 6.056641713302808E-4 0.0 0.0 3.6339850279816846E-4 0.0 26-27 6.056641713302808E-4 0.0 0.0 7.873634227293651E-4 0.0 28-29 7.267970055963369E-4 0.0 0.0 0.0015747268454587302 0.0 30-31 7.267970055963369E-4 0.0 0.0 0.0032705865251835163 0.0 32-33 7.267970055963369E-4 0.0 0.0 0.00599607529616978 0.0 34-35 7.873634227293651E-4 0.0 0.0 0.008721564067156044 0.0 36-37 8.479298398623932E-4 0.0 0.0 0.011931584175206532 0.0 38-39 8.479298398623932E-4 0.0 0.0 0.016292366208784555 0.0 40-41 8.479298398623932E-4 0.0 0.0 0.01986578481963321 0.0 42-43 9.690626741284494E-4 0.0 0.0 0.02840564963539017 0.0 44-45 0.0010901955083945055 0.0 0.0 0.040034401724931556 0.0 46-47 0.0014535940111926739 0.0 0.0 0.05208711873440415 0.0 48-49 0.00157472684545873 0.0 0.0 0.06844005136032172 0.0 50-51 0.00157472684545873 0.0 0.0 0.08709450783729437 0.0 52-53 0.00157472684545873 0.0 0.0 0.11459166121568912 0.0 54-55 0.00157472684545873 0.0 0.0 0.1417254160912857 0.0 56-57 0.00157472684545873 0.0 0.0 0.1909053468033045 0.0 58-59 0.00157472684545873 0.0 0.0 0.24698984906848848 0.0 60-61 0.00157472684545873 0.0 0.0 0.30234755432807614 0.0 62-63 0.00157472684545873 0.0 0.0 0.375330086973375 0.0 64-65 0.00157472684545873 0.0 0.0 0.43831916079172417 0.0 66-67 0.0016958596797247863 0.0 0.0 0.5277151924800736 0.0 68-69 0.0016958596797247863 0.0 0.0 0.6350994500569325 0.0 70-71 0.0016958596797247863 0.0 0.0 0.7418174770453279 0.0 72-73 0.0016958596797247863 0.0 0.0 0.8783947476803062 0.0 74-75 0.0016958596797247863 0.0 0.0 1.0178792063376698 0.0 76-77 0.0016958596797247863 0.0 0.0 1.147370206168084 0.0 78-79 0.0016958596797247863 0.0 0.0 1.29230564236742 0.0 80-81 0.0018169925139908424 0.0 0.0 1.5089517164522617 0.0 82-83 0.0018169925139908424 0.0 0.0 1.7203890786636626 0.0 84-85 0.0018169925139908424 0.0 0.0 1.9722242411027935 0.0 86-87 0.0018775589311238704 0.0 0.0 2.2511325920003875 0.0 88-89 0.0019381253482568985 0.0 0.0 2.6210722678489233 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2195 0.0 47.881767 1 GTATCAA 3715 0.0 39.42796 1 ATCAACG 4785 0.0 30.298368 3 TATCAAC 4835 0.0 29.985046 2 TCAACGC 4830 0.0 29.822884 4 CAACGCA 4870 0.0 29.773167 5 AACGCAG 5065 0.0 28.791899 6 CGTTCGC 35 0.008382246 27.149733 46-47 TACCTGG 620 0.0 26.06685 2 ACGCAGA 5650 0.0 25.7314 7 CGCAGAG 5705 0.0 25.230433 8 TGGTATC 1155 0.0 24.692818 2 GGACCGA 100 0.001652856 23.75097 6 GCAGAGT 5970 0.0 23.47391 9 GAGTACT 3765 0.0 23.215466 12-13 GTGGTAT 1275 0.0 23.125648 1 GTACATG 3105 0.0 22.36164 1 GTACCTG 725 0.0 22.302488 1 TACATGG 3120 0.0 22.09097 2 GGGCGCG 130 2.9360995E-4 21.927965 7 >>END_MODULE