FastQCFastQC Report
Thu 26 May 2016
SRR937883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825540
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55590.6733774256850061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53970.6537539065339051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34790.4214211304116094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.16268139641931342No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.11386486421009279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.057.0711251
GTATCAA42900.035.3424871
ATCAACG55350.027.3003313
TATCAAC56100.027.2741623
GGATCGC700.00839453127.1400016
CAACGCA56900.026.7236715
TCAACGC56800.026.6870614
AACGCAG58100.026.2407846
TGGTATC11700.025.5866322
ACGCAGA65650.023.1520527
GAGTACT46550.023.06019412-13
ACGTCTA1652.4205074E-623.0390435
CGCAGAG66000.022.9573088
GTGGTAT12750.022.3668481
TGCACCG1703.1368527E-622.3614255
GTACTTT48750.021.87205914-15
CCTACGT1754.0336618E-621.7225272
GCACCGT1754.050651E-621.7120026
GTACATG30500.021.6610071
CAGAGTA67850.021.56130810-11
Produced by FastQC (version 0.11.5)