##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937883_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 825540 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954715701237976 31.0 31.0 34.0 30.0 34.0 2 32.25938052668556 33.0 31.0 34.0 31.0 34.0 3 32.34057344283742 34.0 31.0 34.0 30.0 34.0 4 36.024734113428785 37.0 35.0 37.0 35.0 37.0 5 35.91955326210723 37.0 35.0 37.0 35.0 37.0 6 35.95758049276837 37.0 35.0 37.0 35.0 37.0 7 35.895602878116144 37.0 35.0 37.0 35.0 37.0 8 35.87300433655547 37.0 35.0 37.0 35.0 37.0 9 37.543410373815924 39.0 37.0 39.0 35.0 39.0 10-11 37.45478535261768 39.0 37.0 39.0 34.5 39.0 12-13 37.32759163698912 39.0 37.0 39.0 34.0 39.0 14-15 38.49201855755021 40.0 38.0 41.0 34.0 41.0 16-17 38.43667841655159 40.0 38.0 41.0 34.0 41.0 18-19 38.425247716646076 40.0 38.0 41.0 34.0 41.0 20-21 38.3298398623931 40.0 38.0 41.0 34.0 41.0 22-23 38.264694018460645 40.0 38.0 41.0 34.0 41.0 24-25 38.27699081813117 40.0 38.0 41.0 34.0 41.0 26-27 38.11901846064394 40.0 37.5 41.0 33.0 41.0 28-29 37.937810402887806 40.0 37.0 41.0 33.0 41.0 30-31 37.9774547568864 40.0 37.0 41.0 33.0 41.0 32-33 37.87215761804394 40.0 37.0 41.0 33.0 41.0 34-35 37.71617244470286 40.0 37.0 41.0 33.0 41.0 36-37 37.546031082685275 40.0 36.5 41.0 33.0 41.0 38-39 37.40452552268818 40.0 36.0 41.0 32.5 41.0 40-41 37.20110836543354 40.0 36.0 41.0 32.0 41.0 42-43 36.96136468251084 39.0 36.0 41.0 31.0 41.0 44-45 36.636760786878895 39.0 35.5 41.0 30.5 41.0 46-47 36.22154710855925 39.0 35.0 41.0 30.0 41.0 48-49 35.84658647673038 39.0 35.0 40.0 28.5 41.0 50-51 35.54958693703516 39.0 35.0 40.0 27.5 41.0 52-53 35.30223005547884 39.0 35.0 40.0 27.0 41.0 54-55 35.055373452528045 38.0 34.5 40.0 26.5 41.0 56-57 34.76384003197907 38.0 34.0 40.0 26.0 41.0 58-59 34.49888618358892 38.0 34.0 40.0 26.0 41.0 60-61 34.15375390653391 37.5 33.5 40.0 24.5 41.0 62-63 33.75064442667829 37.0 33.0 40.0 23.0 41.0 64-65 33.31644862756499 36.5 32.5 39.0 22.0 41.0 66-67 32.877632822152776 36.0 32.0 39.0 21.5 40.0 68-69 32.42673159386583 35.5 31.5 39.0 20.0 40.0 70-71 32.01094192891925 35.0 31.0 38.0 18.5 40.0 72-73 31.483776679506747 35.0 31.0 37.5 17.5 39.5 74-75 30.992240836301086 34.5 30.0 37.0 14.0 39.0 76-77 29.774676575332506 33.5 28.5 35.5 13.0 38.0 78-79 30.37489037478499 34.0 30.0 36.0 11.0 38.0 80-81 30.162617196017152 34.0 30.0 35.5 7.5 37.5 82-83 29.76583387843109 34.0 29.5 35.0 6.5 37.0 84-85 29.341041621241853 34.0 29.0 35.0 2.0 36.5 86-87 28.91856178985876 34.0 29.0 35.0 2.0 36.0 88-89 28.431690772100687 34.0 28.5 35.0 2.0 36.0 90-91 27.820945684037113 33.0 27.0 35.0 2.0 35.0 92-93 27.297009835986145 33.0 26.0 35.0 2.0 35.0 94-95 26.74189015674589 32.5 25.0 34.5 2.0 35.0 96-97 26.087409453206387 32.0 24.5 34.0 2.0 35.0 98-99 25.069438185914674 32.0 20.5 34.0 2.0 35.0 100-101 22.319192891925283 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27884840738643035 1101 2 0.20393816831859368 1101 3 0.20773383687276947 1101 4 0.10429092036319787 1101 5 0.0065707985146445935 1101 6 0.010546963844284107 1101 7 0.17236556273097392 1101 8 0.05591322334893789 1101 9 0.04261493214505663 1101 10-11 0.2106737551345219 1101 12-13 0.17205618850064752 1101 14-15 0.2081773138028069 1101 16-17 0.19979787086845846 1101 18-19 0.2325351242759055 1101 20-21 0.12166311061135815 1101 22-23 -0.04091140145286687 1101 24-25 0.1396530741878408 1101 26-27 0.0739134403917987 1101 28-29 0.008753752555819005 1101 30-31 -0.046411104525518 1101 32-33 0.13094603040106279 1101 34-35 0.10987996109404463 1101 36-37 -0.02711213543961577 1101 38-39 0.04391352283570171 1101 40-41 0.1986846116371197 1101 42-43 0.16796528083823148 1101 44-45 0.23825252906110705 1101 46-47 0.20231334191059602 1101 48-49 0.07593226509153794 1101 50-51 0.24895985095623985 1101 52-53 0.49804404816148207 1101 54-55 0.27726823017389535 1101 56-57 0.33606877818647973 1101 58-59 0.39885836150534004 1101 60-61 0.302211820986102 1101 62-63 0.07065410189827048 1101 64-65 0.29480486147395624 1101 66-67 0.05164249159847856 1101 68-69 0.21078318759207093 1101 70-71 0.36793217776076403 1101 72-73 -0.04007848267699643 1101 74-75 0.022109709615634188 1101 76-77 0.14227032722057942 1101 78-79 0.24923237531903908 1101 80-81 0.3054388111836097 1101 82-83 0.25849148958909396 1101 84-85 0.14001887901534715 1101 86-87 0.09725763684404853 1101 88-89 0.31019787947050403 1101 90-91 0.1461400400580608 1101 92-93 0.08852866454004626 1101 94-95 -0.25378873447558803 1101 96-97 -0.2799586277843993 1101 98-99 -0.8991759851672789 1101 100-101 -0.5617070866911682 1102 1 0.3874963623458605 1102 2 0.21645748719037528 1102 3 0.22946066802583687 1102 4 0.163230728278414 1102 5 0.005432364999158779 1102 6 0.10256688988059892 1102 7 0.04437458740392941 1102 8 0.05071782676009917 1102 9 0.11464327568979371 1102 10-11 0.18017236141592008 1102 12-13 0.19090243787968575 1102 14-15 0.42227181068419384 1102 16-17 0.3261467420943802 1102 18-19 0.25789286838364944 1102 20-21 0.1573599009188058 1102 22-23 0.2684527316817622 1102 24-25 0.19466011177684805 1102 26-27 0.21376309817178196 1102 28-29 0.09714276563745727 1102 30-31 0.07395409114705132 1102 32-33 0.20248450255429162 1102 34-35 0.11911507176358782 1102 36-37 0.01286930464427627 1102 38-39 0.08354998306642614 1102 40-41 0.13606904338629988 1102 42-43 0.2313191059748405 1102 44-45 0.23036628961749273 1102 46-47 0.26034412791268835 1102 48-49 0.21918342422384285 1102 50-51 0.13933560301232006 1102 52-53 0.2968828459756949 1102 54-55 0.09720427090910988 1102 56-57 0.17005584834158327 1102 58-59 -0.07496974193341543 1102 60-61 -0.02103430144035201 1102 62-63 -0.24418831130479646 1102 64-65 -0.08777779569886945 1102 66-67 -0.3065431903576936 1102 68-69 -0.31331020535378684 1102 70-71 -0.41984829085986775 1102 72-73 -0.6127467638493762 1102 74-75 -0.7460431302585206 1102 76-77 -0.298410387025708 1102 78-79 -0.31824395906303593 1102 80-81 -0.22040535451710852 1102 82-83 -0.0673195580457211 1102 84-85 -0.03242102549422654 1102 86-87 -0.20419610825723922 1102 88-89 -0.34826861608615545 1102 90-91 -0.29686411349937103 1102 92-93 -0.2965619961653694 1102 94-95 -0.5934817714022316 1102 96-97 -0.6332473207247311 1102 98-99 -0.5562211637288499 1102 100-101 -0.47080834517767656 1103 1 0.30740714262249824 1103 2 0.24658058225249135 1103 3 0.24117273644522186 1103 4 0.1591159744685342 1103 5 0.007087417263967666 1103 6 0.16689923774953286 1103 7 0.026893592788788112 1103 8 0.13024920324269829 1103 9 0.03424917791765125 1103 10-11 0.0687002799743226 1103 12-13 0.1531437534746587 1103 14-15 0.17943050564776541 1103 16-17 0.13762168314249124 1103 18-19 0.2066995325866543 1103 20-21 -0.017551891218602123 1103 22-23 -0.04646473567473208 1103 24-25 0.06354891267783813 1103 26-27 -0.05330149374037063 1103 28-29 0.04737703340221344 1103 30-31 0.17228914024429542 1103 32-33 0.23706506426899665 1103 34-35 0.1789604770358153 1103 36-37 0.22458951194375487 1103 38-39 0.2509014155093183 1103 40-41 0.2664374046997864 1103 42-43 0.3405356177828196 1103 44-45 0.572462337201344 1103 46-47 0.5131335640862744 1103 48-49 0.3661361835241621 1103 50-51 0.22353869666663684 1103 52-53 0.3535659663783264 1103 54-55 0.05778459505651057 1103 56-57 0.3306656484330901 1103 58-59 0.34767460220482604 1103 60-61 0.2943512615061863 1103 62-63 0.2796071344418678 1103 64-65 0.3712075279205962 1103 66-67 0.315796935640968 1103 68-69 0.31609534844201903 1103 70-71 0.14610281426806537 1103 72-73 0.2006760091770552 1103 74-75 0.3035723626496605 1103 76-77 0.07899828154149802 1103 78-79 0.3026520656439686 1103 80-81 0.35960973540651864 1103 82-83 0.11783050530555883 1103 84-85 0.2447951695573316 1103 86-87 0.005314943022106888 1103 88-89 -0.1443711923716613 1103 90-91 -0.13385928296405325 1103 92-93 -0.3310888852654301 1103 94-95 -0.31231579983991864 1103 96-97 -0.48143718979874706 1103 98-99 -0.23253174339308558 1103 100-101 -0.48463696235995 1104 1 0.2980234194978202 1104 2 0.21936908089622875 1104 3 0.21455106267564616 1104 4 0.1434007592265374 1104 5 0.125581920375069 1104 6 0.1592095084674412 1104 7 0.060979092438103066 1104 8 0.1068031341590654 1104 9 0.11525723664126986 1104 10-11 0.1278078206974982 1104 12-13 0.19570541918832163 1104 14-15 0.2949660121981168 1104 16-17 0.2366896203514841 1104 18-19 0.0757789339708026 1104 20-21 0.02766230858479446 1104 22-23 -0.014010134653375417 1104 24-25 -0.050505264704220565 1104 26-27 -0.0465846701337469 1104 28-29 -0.008840521151206815 1104 30-31 0.11704082959050055 1104 32-33 0.24896639912713425 1104 34-35 0.1835881166832749 1104 36-37 0.1233228191752005 1104 38-39 0.08998636589225839 1104 40-41 0.07615885622772822 1104 42-43 0.13322952993963355 1104 44-45 0.14844052428428256 1104 46-47 -0.06498704354316942 1104 48-49 0.23318076423674228 1104 50-51 0.15668114819739287 1104 52-53 0.048875794866376054 1104 54-55 -0.03830885346314261 1104 56-57 0.17594716857708192 1104 58-59 -0.014596149501414857 1104 60-61 0.2441866224305329 1104 62-63 0.22440220174812708 1104 64-65 -0.0630050071831576 1104 66-67 -0.11207424871818006 1104 68-69 0.020317094320404294 1104 70-71 -0.19221907836303131 1104 72-73 0.06779016363076096 1104 74-75 -0.06176808391798971 1104 76-77 -0.07633565833214462 1104 78-79 0.08751456596085205 1104 80-81 0.11408062288014165 1104 82-83 -0.08055493322026663 1104 84-85 0.06983664621069607 1104 86-87 0.1769868817070801 1104 88-89 -0.12234476561703644 1104 90-91 -0.03597128260350857 1104 92-93 -0.14795333477376715 1104 94-95 -0.08668252387440845 1104 96-97 -0.04884146956969104 1104 98-99 -0.015098028577824607 1104 100-101 -0.3018261832185516 1105 1 0.1983235053714516 1105 2 0.2517446978180473 1105 3 0.19607722267740968 1105 4 0.10024925989811351 1105 5 0.0681755556606376 1105 6 0.06940943989435766 1105 7 0.11147310173006986 1105 8 0.11035603766290336 1105 9 0.1810638039293906 1105 10-11 0.2749147758324568 1105 12-13 0.30565158302766093 1105 14-15 0.3622258255409321 1105 16-17 0.21334803518088563 1105 18-19 0.24417224778073177 1105 20-21 0.10203893837508105 1105 22-23 0.07963851952123946 1105 24-25 0.05992020296105949 1105 26-27 -0.14342134352141755 1105 28-29 -0.23388762582581535 1105 30-31 -0.05704829654307986 1105 32-33 -0.011383260681178342 1105 34-35 0.1553603062092037 1105 36-37 0.12867104775633464 1105 38-39 0.03481727662457246 1105 40-41 0.054139583416954906 1105 42-43 0.0903630964633848 1105 44-45 -0.01865603479393485 1105 46-47 -0.15499235952982815 1105 48-49 -0.270522732675893 1105 50-51 -0.19742450782366916 1105 52-53 -0.1261337990740259 1105 54-55 -0.13590871694609064 1105 56-57 -0.1488690524169698 1105 58-59 -0.14777341091900098 1105 60-61 -0.09248322648849694 1105 62-63 -0.2992771373080174 1105 64-65 -0.4345087236554761 1105 66-67 -0.08418340574110061 1105 68-69 -0.024370221861275354 1105 70-71 -0.27721942437399605 1105 72-73 -0.287005155155434 1105 74-75 -0.21546497516674634 1105 76-77 -0.4800148558383519 1105 78-79 -0.17541366192826402 1105 80-81 -0.40493425244581616 1105 82-83 0.021478195756895246 1105 84-85 0.022590421662162186 1105 86-87 -0.1402615290016449 1105 88-89 -0.11388440431867863 1105 90-91 0.13304170538991045 1105 92-93 0.1951719778156864 1105 94-95 -0.08395926029898249 1105 96-97 0.18760591002635962 1105 98-99 0.31822613022233526 1105 100-101 0.32748833760344453 1106 1 0.14535355793143978 1106 2 0.1143470096992587 1106 3 0.0660374590788706 1106 4 0.013149680283234488 1106 5 0.11703407023667722 1106 6 -0.04878062915875603 1106 7 -0.030374886629950026 1106 8 -0.0571036892526493 1106 9 0.004580252030550014 1106 10-11 -0.017784290978916317 1106 12-13 -0.3102025523391845 1106 14-15 -0.09547262514794141 1106 16-17 -0.039257452100919465 1106 18-19 0.1786938286562929 1106 20-21 0.21384858495773074 1106 22-23 0.17324056125643494 1106 24-25 0.041733925431053365 1106 26-27 0.2779436521894283 1106 28-29 0.22576108955876606 1106 30-31 0.19451452188561547 1106 32-33 0.19569077354679365 1106 34-35 -0.22086127514029386 1106 36-37 -0.015607069942021212 1106 38-39 0.15015659424520322 1106 40-41 -0.004265867845383298 1106 42-43 -0.16522051373012658 1106 44-45 0.1992712343141534 1106 46-47 0.002035939450728108 1106 48-49 0.07929681452146298 1106 50-51 0.26748909284364686 1106 52-53 0.3721890443787217 1106 54-55 0.26155368773265053 1106 56-57 0.2596296466166592 1106 58-59 0.11287129019049758 1106 60-61 0.02367897013041187 1106 62-63 -0.034681550640911496 1106 64-65 -0.05013892218910598 1106 66-67 -0.02715060432687011 1106 68-69 0.3384264055789288 1106 70-71 0.1555728665116547 1106 72-73 0.07061663567571941 1106 74-75 0.08084788710144153 1106 76-77 0.04442032570451815 1106 78-79 0.006488213453852865 1106 80-81 -0.11032579829717903 1106 82-83 -0.2810936417573835 1106 84-85 -0.33820380763967606 1106 86-87 0.06676333391109779 1106 88-89 0.3450077837219112 1106 90-91 0.12329630616622467 1106 92-93 0.1565262861707808 1106 94-95 0.12282448544148039 1106 96-97 0.1670712578749054 1106 98-99 -0.16567056025193594 1106 100-101 -0.23783842059691196 1107 1 0.15568568127775606 1107 2 0.06824891633328889 1107 3 0.009388660825116801 1107 4 -0.08373771856626888 1107 5 0.08216503826648847 1107 6 0.050411674359047254 1107 7 -0.05187878793360312 1107 8 0.007300985075929134 1107 9 -0.13016245488326206 1107 10-11 -0.07843162398261683 1107 12-13 0.040999947630659506 1107 14-15 0.0432776612692507 1107 16-17 -0.16819644222405117 1107 18-19 0.071689873084118 1107 20-21 -0.07011597166845718 1107 22-23 0.1607919656051493 1107 24-25 0.03392528829449759 1107 26-27 0.03289188605057092 1107 28-29 0.05319774613550976 1107 30-31 0.07668248898684027 1107 32-33 0.20503475757978862 1107 34-35 0.18953461371778246 1107 36-37 0.1688817808919012 1107 38-39 -0.011781425074389063 1107 40-41 0.11593890537250218 1107 42-43 0.18989308837308272 1107 44-45 0.23587309029755943 1107 46-47 0.025392507750680693 1107 48-49 0.046137994680890415 1107 50-51 0.2753429537473906 1107 52-53 0.18687184487558284 1107 54-55 0.05871604708673317 1107 56-57 0.03707067482423554 1107 58-59 0.10872050209098205 1107 60-61 0.22772182417221387 1107 62-63 0.04209031655798867 1107 64-65 0.05132361444214695 1107 66-67 0.0729635103123698 1107 68-69 0.07245337100384575 1107 70-71 0.08482405137435478 1107 72-73 0.18969910573758142 1107 74-75 0.22692291634614392 1107 76-77 0.17918112212520398 1107 78-79 0.2395586962024936 1107 80-81 0.36926923794213806 1107 82-83 0.26703575248533085 1107 84-85 0.5015917507834011 1107 86-87 0.5888144427978652 1107 88-89 0.580605521327584 1107 90-91 0.5633687847904554 1107 92-93 0.19598100813506036 1107 94-95 0.23833578535344557 1107 96-97 0.5377041494222858 1107 98-99 1.014548413228848 1107 100-101 0.9871249481516564 1108 1 0.12726524126568606 1108 2 0.02306217704978053 1108 3 0.05722488494099309 1108 4 -0.027335126202537197 1108 5 0.01287068139973968 1108 6 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0.46507969727293386 2316 72-73 0.3874088939069722 2316 74-75 0.35331737062457336 2316 76-77 0.2993935362939304 2316 78-79 0.47545245994036023 2316 80-81 0.21149530730741972 2316 82-83 0.25294410510841203 2316 84-85 0.20465565982656386 2316 86-87 -0.3770183880294198 2316 88-89 -0.1437715332737497 2316 90-91 0.04055663127648046 2316 92-93 0.2628248487077194 2316 94-95 0.12477955006390218 2316 96-97 0.5262324226818293 2316 98-99 0.56184679483518 2316 100-101 0.7179369168730894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 2.0 5 21.0 6 46.0 7 111.0 8 284.0 9 532.0 10 888.0 11 1457.0 12 2071.0 13 2919.0 14 3791.0 15 5263.0 16 7070.0 17 9743.0 18 9022.0 19 5907.0 20 4958.0 21 5077.0 22 5318.0 23 5783.0 24 6765.0 25 8087.0 26 9777.0 27 11772.0 28 14756.0 29 17751.0 30 21964.0 31 27311.0 32 34067.0 33 43580.0 34 58866.0 35 82806.0 36 120611.0 37 171119.0 38 118042.0 39 7997.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21297671634847 20.09714242790646 15.869544673643604 21.820336182101467 2 15.63667417690239 20.9785110352012 40.15456549652349 23.23024929137292 3 19.061826198609396 24.891101581994814 31.137195048089733 24.909877171306054 4 12.914334859607045 17.969692564866634 37.108438113234975 32.007534462291346 5 13.124156299910087 37.73487277547944 34.61321273723574 14.527758187374731 6 30.808319403057393 37.22714829081571 17.292923419822177 14.671608886304721 7 27.241320832424837 33.746759696683384 20.947622162463357 18.064297308428422 8 25.995469631998446 36.08607699202946 19.417956731351598 18.50049664462049 9 25.619231048768075 17.03321462315575 20.81122659107978 36.53632773699639 10-11 24.62152045933571 27.511144220752477 27.010744482399403 20.856590837512414 12-13 25.398482869900402 24.90951004595969 28.19997674153792 21.492030342601993 14-15 22.386256268624173 26.278981030598153 26.81033020810621 24.524432492671462 16-17 21.172081304358358 29.432432105046395 27.728759357511446 21.666727233083797 18-19 21.025026043559368 27.3349565133125 30.879060978268768 20.760956464859365 20-21 21.708639193739856 26.382670736729896 31.416163965404465 20.492526104125787 22-23 21.50695368931345 26.317724427733356 31.316131517455997 20.8591903654972 24-25 21.373127132697547 26.528365104495364 31.351032476963535 20.747475285843553 26-27 21.20356473590937 26.773070908300234 31.203613189571318 20.819751166219074 28-29 20.99831260531075 26.852590992728338 31.33914695112128 20.80994945083964 30-31 21.157000266492236 26.576846670058384 31.339365748479782 20.926787314969594 32-33 20.905407369721637 26.828076168326188 31.18625384596749 21.080262615984687 34-35 21.432698597281778 26.67847711800761 31.053431693194756 20.835392591515856 36-37 21.489812728638224 26.54026455411004 30.972090994985102 20.99783172226664 38-39 21.306114785473753 26.839462654747198 31.019999030937328 20.83442352884173 40-41 21.347242772980525 26.863816660922886 30.905884155703518 20.883056410393074 42-43 21.239432831698235 26.841152654240076 30.773992832861257 21.145421681200435 44-45 21.401991423795334 27.08893572691814 30.441953145819706 21.067119703466823 46-47 21.501623179979166 27.401337306490298 29.89812728638225 21.19891222714829 48-49 21.697192140901713 27.446459287254406 29.618068173559127 21.238280398284758 50-51 21.688773408920223 27.683940208833008 29.393245633161325 21.234040749085448 52-53 21.820626499018825 27.861339244615646 29.139472345373935 21.178561910991593 54-55 21.732986893427334 27.857099595416337 29.094592630278367 21.315320880877973 56-57 21.663820045061414 28.108207960849867 29.061281100855197 21.16669089323352 58-59 21.80318337088451 27.710528865954405 29.1595803958621 21.32670736729898 60-61 21.684594326138043 27.699929742956126 29.50032705865252 21.115148872253314 62-63 21.53705453400199 27.819487850376724 29.537817670857862 21.10563994476343 64-65 21.56237129636359 27.59860212709257 29.45157109286043 21.387455483683407 66-67 21.532027521379945 27.88786733531991 29.234985585192724 21.34511955810742 68-69 21.51137437313758 27.817731424279867 29.2193594495724 21.45153475301015 70-71 21.546200062989072 27.891985851684957 29.03051336095162 21.53130072437435 72-73 21.600830971243067 27.855827700656537 28.97388376093224 21.569457567168158 74-75 21.612459723332606 28.070475082975992 28.667841655159048 21.649223538532354 76-77 21.63190154323231 27.949099983041403 28.8428786006735 21.57611987305279 78-79 21.645044455750178 28.011180560602757 28.688010272064346 21.655764711582723 80-81 21.570366063425155 28.1689560772343 28.81792523681469 21.442752622525862 82-83 21.663619135066316 28.160039925793264 28.62737521221177 21.54896572692865 84-85 21.538642306031388 28.089812783034606 28.70432002345133 21.667224887482668 86-87 21.655764711582723 27.920936599074548 28.734222448336844 21.689076241005885 88-89 21.511858904474646 27.879085204835626 28.63489352423868 21.97416236645105 90-91 21.44245155584631 27.980458788396422 28.786276327027565 21.7908133287297 92-93 21.535663322536177 27.84539796444933 28.724352715723068 21.894585997291426 94-95 21.314533517455242 27.916515250623835 28.676744918477603 22.09220631344332 96-97 21.37594786442813 27.906400658962617 28.579838651064758 22.137812825544493 98-99 21.370271219701284 28.045740312284227 28.40296536770317 22.181023100311315 100-101 21.451565027229716 27.844399886113752 28.711995008390023 21.992040078266506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 623.0 1 443.0 2 338.0 3 454.0 4 376.5 5 281.0 6 522.0 7 1173.5 8 1878.5 9 2261.0 10 2030.0 11 1594.5 12 1580.0 13 1684.0 14 1792.0 15 1869.5 16 1888.5 17 1971.0 18 2091.5 19 2181.0 20 2314.0 21 2578.5 22 3006.5 23 3644.5 24 4292.5 25 5084.0 26 6233.5 27 7533.0 28 8672.0 29 10001.5 30 11631.5 31 12868.5 32 13903.0 33 15083.5 34 16713.0 35 18423.5 36 20488.0 37 22434.0 38 23484.5 39 24117.5 40 24933.5 41 26459.5 42 28054.0 43 29514.0 44 30593.0 45 31208.0 46 31029.5 47 31089.5 48 32110.0 49 31804.5 50 30533.0 51 28801.0 52 27016.0 53 25842.0 54 24399.0 55 22267.0 56 19392.0 57 16817.5 58 15029.5 59 13268.0 60 11127.0 61 8961.0 62 6913.5 63 5046.5 64 3711.5 65 2757.0 66 1977.5 67 1558.0 68 1150.5 69 753.0 70 536.0 71 411.0 72 342.0 73 261.5 74 213.5 75 163.0 76 89.5 77 56.0 78 38.0 79 28.5 80 17.0 81 9.0 82 9.5 83 9.0 84 5.0 85 3.0 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01865445647697265 2 0.0 3 0.0 4 0.0 5 0.03706664728541319 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004118516365045909 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.47929839862393E-4 24-25 1.2113283426605617E-4 26-27 0.0010901955083945055 28-29 8.47929839862393E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011507619255275334 42-43 0.012840080432201953 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010296290912614773 84-85 6.0566417133028086E-5 86-87 0.0 88-89 0.0 90-91 0.012718947597935897 92-93 0.001695859679724786 94-95 0.0 96-97 0.0 98-99 0.0012113283426605616 100-101 0.018775589311238704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 825540.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.78149833165858 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62857864921793 55.01213206892508 2 9.784508878536213 12.87279308939057 3 2.6501673863256805 5.229959445065962 4 1.2026077688561236 3.16437363762595 5 0.6715286865679122 2.2087081587563997 6 0.43196074556365405 1.7049015038182502 7 0.2974235694297773 1.3695477625368588 8 0.20678001588128697 1.088183941577217 9 0.1712624023301198 1.0139307689839154 >10 0.8957433746992353 10.498315053395022 >50 0.04264165792334459 1.8956152555027146 >100 0.015504798167793068 1.7757689813032298 >500 5.537427860758401E-4 0.25000398698726084 >1k 3.691618573838935E-4 0.5854877247462709 >5k 3.691618573838935E-4 1.3302786213853472 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5559 0.6733774256850061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5397 0.6537539065339051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3479 0.4214211304116094 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1343 0.16268139641931342 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.11386486421009279 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.4226566853211232E-4 0.0 14-15 0.0 0.0 0.0 2.4226566853211232E-4 0.0 16-17 0.0 0.0 0.0 2.4226566853211232E-4 0.0 18-19 0.0 0.0 0.0 2.4226566853211232E-4 0.0 20-21 0.0 0.0 0.0 3.028320856651404E-4 0.0 22-23 0.0 0.0 0.0 4.8453133706422464E-4 0.0 24-25 0.0 0.0 0.0 6.056641713302808E-4 0.0 26-27 0.0 0.0 0.0 0.0010296290912614773 0.0 28-29 2.4226566853211232E-4 0.0 0.0 0.0018169925139908424 0.0 30-31 2.4226566853211232E-4 0.0 0.0 0.003633985027981685 0.0 32-33 2.4226566853211232E-4 0.0 0.0 0.006480606633234005 0.0 34-35 2.4226566853211232E-4 0.0 0.0 0.009266661821353297 0.0 36-37 2.4226566853211232E-4 0.0 0.0 0.012537248346536812 0.0 38-39 2.4226566853211232E-4 0.0 0.0 0.016958596797247864 0.0 40-41 2.4226566853211232E-4 0.0 0.0 0.020532015408096516 0.0 42-43 4.239649199311966E-4 0.0 0.0 0.02858734888678925 0.0 44-45 8.479298398623932E-4 0.0 0.0 0.039973835307798536 0.0 46-47 0.0011507619255275337 0.0 0.0 0.05184485306587204 0.0 48-49 0.0012113283426605616 0.0 0.0 0.0679555200232575 0.0 50-51 0.0012113283426605616 0.0 0.0 0.0864888436659641 0.0 52-53 0.0012113283426605616 0.0 0.0 0.11392543062722582 0.0 54-55 0.0012113283426605616 0.0 0.0 0.14093805266855636 0.0 56-57 0.0012113283426605616 0.0 0.0 0.1896334520435109 0.0 58-59 0.0012113283426605616 0.0 0.0 0.24620248564575914 0.0 60-61 0.0012113283426605616 0.0 0.0 0.301863022991012 0.0 62-63 0.0012113283426605616 0.0 0.0 0.3744821571335126 0.0 64-65 0.0013324611769266177 0.0 0.0 0.437955762288926 0.0 66-67 0.0013324611769266177 0.0 0.0 0.5274729268115416 0.0 68-69 0.0013324611769266177 0.0 0.0 0.6354628485597307 0.0 70-71 0.001393027594059646 0.0 0.0 0.7434527703079197 0.0 72-73 0.0014535940111926739 0.0 0.0 0.8813625021198246 0.0 74-75 0.0014535940111926739 0.0 0.0 1.022361121205514 0.0 76-77 0.0014535940111926739 0.0 0.0 1.1535479807156528 0.0 78-79 0.0014535940111926739 0.0 0.0 1.300421542263246 0.0 80-81 0.0014535940111926739 0.0 0.0 1.518400077525014 0.0 82-83 0.0014535940111926739 0.0 0.0 1.7302614046563463 0.0 84-85 0.0014535940111926739 0.0 0.0 1.9831867626038715 0.0 86-87 0.0015141604283257018 0.0 0.0 2.266395329117911 0.0 88-89 0.00157472684545873 0.0 0.0 2.6414831504227534 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2665 0.0 57.071125 1 GTATCAA 4290 0.0 35.342487 1 ATCAACG 5535 0.0 27.300331 3 TATCAAC 5610 0.0 27.274162 3 GGATCGC 70 0.008394531 27.140001 6 CAACGCA 5690 0.0 26.723671 5 TCAACGC 5680 0.0 26.687061 4 AACGCAG 5810 0.0 26.240784 6 TGGTATC 1170 0.0 25.586632 2 ACGCAGA 6565 0.0 23.152052 7 GAGTACT 4655 0.0 23.060194 12-13 ACGTCTA 165 2.4205074E-6 23.039043 5 CGCAGAG 6600 0.0 22.957308 8 GTGGTAT 1275 0.0 22.366848 1 TGCACCG 170 3.1368527E-6 22.361425 5 GTACTTT 4875 0.0 21.872059 14-15 CCTACGT 175 4.0336618E-6 21.722527 2 GCACCGT 175 4.050651E-6 21.712002 6 GTACATG 3050 0.0 21.661007 1 CAGAGTA 6785 0.0 21.561308 10-11 >>END_MODULE