FastQCFastQC Report
Thu 26 May 2016
SRR937882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714879
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.5593953662088269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34930.4886141570811284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.32383102594984603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9410.13163066756751843No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.1260353150673051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.051.2698861
GTATCAA30900.039.2426151
TATCAAC40100.029.9916272
ATCAACG40650.029.5816843
TCAACGC40850.029.4430524
CAACGCA41400.029.0518975
AACGCAG42150.028.4022466
GTACCGA350.00839268927.14255510-11
CCGTCCG908.933802E-426.3941489
GTACATG24900.025.781651
TACATGG24850.025.0592922
CGCAGAG47900.024.8953578
ACGCAGA48050.024.8176427
GTCTAGG1751.4686339E-724.4557361
ACATGGG24950.024.3838543
CTAAGAC2151.6480044E-924.3174023
GCAGAGT50500.023.425469
GAGTACT32100.023.38144312-13
CGGAGTA1153.6288839E-922.7171414-15
CAATACT3007.2759576E-1222.1850854
Produced by FastQC (version 0.11.5)