##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937882_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714879 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.539790649886204 33.0 31.0 34.0 30.0 34.0 2 31.663607407687174 33.0 31.0 34.0 30.0 34.0 3 31.68500123797174 33.0 31.0 34.0 30.0 34.0 4 35.2779435400956 37.0 35.0 37.0 33.0 37.0 5 35.2484238591426 37.0 35.0 37.0 33.0 37.0 6 35.31747190783335 37.0 35.0 37.0 33.0 37.0 7 35.25027871849642 37.0 35.0 37.0 33.0 37.0 8 35.247341158433805 37.0 35.0 37.0 33.0 37.0 9 36.83517630256309 39.0 37.0 39.0 33.0 39.0 10-11 36.72174242074533 39.0 37.0 39.0 32.5 39.0 12-13 36.58248458830096 39.0 37.0 39.0 32.0 39.0 14-15 37.68840950706343 40.0 37.5 41.0 32.0 41.0 16-17 37.61802207086794 40.0 37.0 41.0 32.0 41.0 18-19 37.570479759511755 40.0 37.0 41.0 32.0 41.0 20-21 37.3407268922433 40.0 37.0 41.0 32.0 41.0 22-23 37.241419876650454 40.0 37.0 41.0 31.5 41.0 24-25 37.102743261447046 39.0 36.5 41.0 31.0 41.0 26-27 37.18304356401573 40.0 37.0 41.0 31.0 41.0 28-29 37.12467214731444 40.0 37.0 41.0 31.0 41.0 30-31 36.89081508898709 39.0 36.0 41.0 30.5 41.0 32-33 36.6294044166915 39.0 35.5 41.0 30.0 41.0 34-35 36.347687510753566 39.0 35.0 40.5 30.0 41.0 36-37 36.35939298818401 39.0 35.0 41.0 30.0 41.0 38-39 36.31374470364915 39.0 35.0 41.0 30.0 41.0 40-41 36.112545619608355 39.0 35.0 41.0 29.5 41.0 42-43 35.86037986848124 39.0 35.0 41.0 28.5 41.0 44-45 35.60107794465917 39.0 35.0 40.5 27.0 41.0 46-47 35.220314906438716 39.0 35.0 40.0 25.5 41.0 48-49 34.857094697144554 38.5 34.5 40.0 24.0 41.0 50-51 33.9425769955475 37.5 33.5 39.5 23.0 40.5 52-53 34.0396899335412 38.0 33.5 39.5 23.0 40.5 54-55 34.32901442062223 38.0 34.0 40.0 23.0 41.0 56-57 34.09306749813605 38.0 34.0 40.0 21.5 41.0 58-59 33.85391793576255 38.0 33.5 40.0 20.5 41.0 60-61 33.54520065633484 38.0 33.0 40.0 19.0 41.0 62-63 33.16634913041228 37.0 33.0 40.0 17.5 41.0 64-65 32.78460550666617 37.0 32.0 39.5 13.5 41.0 66-67 32.36344682107042 36.0 31.5 39.0 10.0 41.0 68-69 31.876390270241536 35.5 31.0 39.0 7.5 40.0 70-71 31.429737060397635 35.0 31.0 38.5 6.5 40.0 72-73 30.946886116391724 35.0 30.0 38.0 2.0 40.0 74-75 30.442121673737795 35.0 30.0 37.0 2.0 39.0 76-77 29.977394076480074 34.5 29.0 36.5 2.0 39.0 78-79 29.415333224223957 34.0 29.0 36.0 2.0 39.0 80-81 28.862329149408502 34.0 28.5 35.5 2.0 37.5 82-83 28.326078958816808 34.0 27.0 35.0 2.0 37.0 84-85 27.830601402475104 34.0 26.0 35.0 2.0 36.5 86-87 27.295559108604394 33.0 26.0 35.0 2.0 36.0 88-89 26.783191281321734 33.0 25.0 35.0 2.0 36.0 90-91 26.245591211939363 32.5 24.5 35.0 2.0 35.0 92-93 25.672496324552824 32.0 21.5 35.0 2.0 35.0 94-95 25.00642346467024 32.0 19.0 34.0 2.0 35.0 96-97 24.20484095909937 31.0 7.5 34.0 2.0 35.0 98-99 23.243167025468644 31.0 2.0 34.0 2.0 35.0 100-101 20.74650465323502 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1775557950611919 1101 2 0.2748146824796329 1101 3 0.20398253641190678 1101 4 0.18412037686588434 1101 5 0.05981138133724784 1101 6 0.09730566040487076 1101 7 -0.012383309282803623 1101 8 0.2863129689498791 1101 9 0.24183244745756838 1101 10-11 0.09372779032011636 1101 12-13 0.1796568357020547 1101 14-15 0.27410321913134084 1101 16-17 0.19535488141401913 1101 18-19 0.2740896918422706 1101 20-21 0.27337481929967566 1101 22-23 0.2116617988572429 1101 24-25 0.2640032258880254 1101 26-27 0.2486043240069975 1101 28-29 0.2641233005901924 1101 30-31 0.21614099922576457 1101 32-33 0.06588792017965517 1101 34-35 0.33088707832002484 1101 36-37 0.18593500374404925 1101 38-39 0.2967577872750482 1101 40-41 0.1685749709836486 1101 42-43 0.07166877025861851 1101 44-45 0.14331102741791568 1101 46-47 0.219314243586183 1101 48-49 0.4623931974174269 1101 50-51 0.5268180078802871 1101 52-53 0.21722615471357187 1101 54-55 0.498604690128424 1101 56-57 0.582505785102974 1101 58-59 0.49164657584655913 1101 60-61 0.45268290208651507 1101 62-63 0.40401821863545706 1101 64-65 0.4985158875829043 1101 66-67 0.43028781825064755 1101 68-69 0.7018043938185805 1101 70-71 0.5247275508994242 1101 72-73 0.4192879915055556 1101 74-75 0.5374973134667087 1101 76-77 0.5111277619541283 1101 78-79 0.5818328814266174 1101 80-81 0.6200824102823077 1101 82-83 0.32100501732007913 1101 84-85 0.5141367756170823 1101 86-87 0.6350279422434753 1101 88-89 0.6723534082122775 1101 90-91 0.6791173612335974 1101 92-93 1.0523932398257472 1101 94-95 1.2987844532230959 1101 96-97 1.2044965894813089 1101 98-99 1.2283745705422433 1101 100-101 1.3691459519523868 1102 1 0.07799285799825384 1102 2 0.12671278437773736 1102 3 0.16085066828003747 1102 4 0.002452045197557595 1102 5 -0.0033629443370770673 1102 6 0.23424372234293145 1102 7 0.01056873866924235 1102 8 -0.06792029778338815 1102 9 0.03462715275227879 1102 10-11 0.12803723087955632 1102 12-13 0.1797879545831762 1102 14-15 0.29625606628418666 1102 16-17 0.33793729899643665 1102 18-19 0.07236641511899933 1102 20-21 0.17966228183713895 1102 22-23 0.18303417647962306 1102 24-25 0.19849997913477324 1102 26-27 0.26847820013087187 1102 28-29 0.2393730508399443 1102 30-31 0.13493470551947695 1102 32-33 0.09442812996986305 1102 34-35 0.1258920733250264 1102 36-37 0.1306777609868064 1102 38-39 0.25736592913318646 1102 40-41 0.31968011583879274 1102 42-43 0.24917876026860597 1102 44-45 0.2432423460992439 1102 46-47 0.42009471311665436 1102 48-49 0.3971834072076348 1102 50-51 0.33405452136679514 1102 52-53 0.39964998088738923 1102 54-55 0.41305898583272693 1102 56-57 0.5686945962917846 1102 58-59 0.48285536705535037 1102 60-61 0.5984808541344719 1102 62-63 0.5068029339201772 1102 64-65 0.42619445901147657 1102 66-67 0.24554755851038834 1102 68-69 0.3064372609514443 1102 70-71 0.5409113670832397 1102 72-73 0.3582490304665953 1102 74-75 0.31889091986031204 1102 76-77 0.5167034362798013 1102 78-79 0.5354292850230209 1102 80-81 0.5807904522403504 1102 82-83 0.4209613110263746 1102 84-85 0.5410286337589412 1102 86-87 0.5317749452404783 1102 88-89 0.5724845270933976 1102 90-91 0.6830821464483883 1102 92-93 0.754703429635935 1102 94-95 0.4752454921841327 1102 96-97 0.4852033826881019 1102 98-99 0.7164302648479364 1102 100-101 0.737221626278064 1103 1 -0.16952238286805255 1103 2 -0.04852253979375831 1103 3 -0.05253553180828874 1103 4 -0.13051585372779329 1103 5 -0.13068425383924165 1103 6 -0.025865258510727074 1103 7 -0.04260651397409276 1103 8 -0.18514886315025336 1103 9 -0.09551826329739299 1103 10-11 -0.13597424523298685 1103 12-13 -0.08049611221684927 1103 14-15 -0.17952091481193833 1103 16-17 -0.21400288060331008 1103 18-19 -0.06162331461541015 1103 20-21 -0.012369656870333756 1103 22-23 -0.14679229629898316 1103 24-25 -0.07499644662228633 1103 26-27 -0.3506465433289776 1103 28-29 -0.029319741789016973 1103 30-31 -0.18023461097963178 1103 32-33 -0.16795967430623193 1103 34-35 -0.18934922595883563 1103 36-37 -0.0448774265364662 1103 38-39 0.15836064462259003 1103 40-41 0.12470337764397499 1103 42-43 0.01614775463121987 1103 44-45 -0.17262953320349084 1103 46-47 -0.0478894550004938 1103 48-49 0.012894921855320263 1103 50-51 -0.2504892593206023 1103 52-53 -0.22124185875444624 1103 54-55 0.06406885559258768 1103 56-57 0.12139032451942455 1103 58-59 0.04940859318304547 1103 60-61 -0.036459535460174664 1103 62-63 -0.14697448448490746 1103 64-65 -0.08334271874377919 1103 66-67 0.19750635621386436 1103 68-69 0.22418908162879703 1103 70-71 0.0819177405364222 1103 72-73 0.036553094408954934 1103 74-75 0.18927752694904854 1103 76-77 0.03182766882424559 1103 78-79 -0.00860902497273841 1103 80-81 0.14349638795160757 1103 82-83 -0.2974060436441732 1103 84-85 0.022583451085033346 1103 86-87 0.09957488072658194 1103 88-89 0.3385006966999917 1103 90-91 0.2754146809351745 1103 92-93 0.40557014566222804 1103 94-95 0.22203008072404273 1103 96-97 0.1664901317939993 1103 98-99 0.45219688691775417 1103 100-101 0.6798689366115411 1104 1 -0.004424724419330062 1104 2 0.11076345592840653 1104 3 0.12620475863413105 1104 4 0.14752225276776443 1104 5 0.11614255016841923 1104 6 0.16815703125624282 1104 7 0.21874944684995512 1104 8 0.35790387804078705 1104 9 0.2530337461588701 1104 10-11 0.22571732855964655 1104 12-13 0.1505983941436142 1104 14-15 0.2566879954661161 1104 16-17 0.35785488141402055 1104 18-19 0.24284871060128665 1104 20-21 0.24742784960270825 1104 22-23 0.27335011054555025 1104 24-25 0.22578172516652018 1104 26-27 0.29946110611377463 1104 28-29 0.2273538200707108 1104 30-31 0.24673262981740152 1104 32-33 0.2443060298477704 1104 34-35 0.2874076410905886 1104 36-37 0.08817165598070176 1104 38-39 0.2627841220513858 1104 40-41 0.3167802379389144 1104 42-43 0.2527654513552946 1104 44-45 0.337259440116334 1104 46-47 0.38644086696280766 1104 48-49 0.313430354704586 1104 50-51 0.45733748839976585 1104 52-53 0.3910266597640657 1104 54-55 0.6277262630000067 1104 56-57 0.7301338514810354 1104 58-59 0.6906545123544987 1104 60-61 0.5926810983347153 1104 62-63 0.6576347410019778 1104 64-65 0.732742078059097 1104 66-67 0.6706467648595975 1104 68-69 0.7606878714520526 1104 70-71 1.1613707687926471 1104 72-73 1.2094395066570627 1104 74-75 1.1099089296283253 1104 76-77 1.1218240101503767 1104 78-79 0.8886420444857812 1104 80-81 0.9564009528508528 1104 82-83 1.0914054502205133 1104 84-85 1.259816790047097 1104 86-87 0.97682989029542 1104 88-89 1.0353584587173295 1104 90-91 1.0548388619551012 1104 92-93 0.8705840767665833 1104 94-95 0.8378446985333419 1104 96-97 0.6474439199286408 1104 98-99 0.865964758132435 1104 100-101 1.0035525176089521 1105 1 -0.4227220241821463 1105 2 -0.3137598974742559 1105 3 -0.26519279655652994 1105 4 -0.39844335569784306 1105 5 -0.31127717250992504 1105 6 -0.31925402512032974 1105 7 -0.3035776832357939 1105 8 -0.17773058647298967 1105 9 -0.35097327810677825 1105 10-11 -0.16184310749216735 1105 12-13 -0.14030426960388098 1105 14-15 -0.23903016727791027 1105 16-17 -0.3484829219065446 1105 18-19 -0.32308360102067724 1105 20-21 -0.14471660396416297 1105 22-23 -0.17490294546612262 1105 24-25 -0.09933002451419526 1105 26-27 -0.18196906173880478 1105 28-29 -0.3009427955103874 1105 30-31 -0.14785777339368877 1105 32-33 -0.18225276416792013 1105 34-35 -0.36650473803731387 1105 36-37 -0.4533581743422346 1105 38-39 -0.48311648915095873 1105 40-41 -0.7020404608386812 1105 42-43 -0.5343400038536075 1105 44-45 -0.5977946102394398 1105 46-47 -0.4456096256480322 1105 48-49 -0.6388536719328854 1105 50-51 -0.5263105685241598 1105 52-53 -0.687397669737841 1105 54-55 -0.6524062401928461 1105 56-57 -0.442262837079447 1105 58-59 -0.34602903079456127 1105 60-61 -0.37045861157223925 1105 62-63 -0.413434635024295 1105 64-65 -0.4292436453490396 1105 66-67 -0.37436669071420425 1105 68-69 -0.10961978226077207 1105 70-71 -0.14639127280560515 1105 72-73 -0.03472214267699414 1105 74-75 0.07243287598847914 1105 76-77 0.00609467243827666 1105 78-79 -0.10603844265160589 1105 80-81 0.253045041520803 1105 82-83 0.12434879997420722 1105 84-85 0.14915609856744183 1105 86-87 0.16366281311972486 1105 88-89 -0.06609428713197119 1105 90-91 0.07684670723881126 1105 92-93 0.10646142579048146 1105 94-95 0.26069750823959836 1105 96-97 0.41310027825741 1105 98-99 0.5604548146914539 1105 100-101 0.28546389327032884 1106 1 -0.1074393173532755 1106 2 -0.02252507453754049 1106 3 -0.01422022856505123 1106 4 -0.04599413228088167 1106 5 -0.18963701972406 1106 6 0.010194921681232927 1106 7 -0.023890082079894626 1106 8 -0.11588644453986063 1106 9 -0.03373660714374438 1106 10-11 -0.06740543129698295 1106 12-13 -0.12177103750000384 1106 14-15 -0.08472585502031649 1106 16-17 0.027113713979304066 1106 18-19 -0.11090192943967025 1106 20-21 -0.0828812901822431 1106 22-23 -0.006411794216347744 1106 24-25 0.07066779787518129 1106 26-27 0.020675263819875056 1106 28-29 0.1608695650783929 1106 30-31 -0.005315501262991518 1106 32-33 0.14357958335519072 1106 34-35 -0.2084160916283011 1106 36-37 0.18385777989262664 1106 38-39 0.12605537141133993 1106 40-41 0.2533361775190457 1106 42-43 0.5560808635416805 1106 44-45 0.3453079552213012 1106 46-47 0.6047108362408409 1106 48-49 0.7641632016067845 1106 50-51 0.7659012364796425 1106 52-53 0.6439228451199313 1106 54-55 0.8944153311003546 1106 56-57 0.6611854387826313 1106 58-59 0.6079292179356557 1106 60-61 0.5541666334089612 1106 62-63 0.8205731690613831 1106 64-65 0.7672562869684612 1106 66-67 0.781261844224673 1106 68-69 0.7220774003819095 1106 70-71 0.598086718613434 1106 72-73 0.969424145673969 1106 74-75 1.0686102865151632 1106 76-77 0.6685149983736203 1106 78-79 0.9948408412087701 1106 80-81 0.9173104510587393 1106 82-83 0.7445352505277327 1106 84-85 0.692596487947764 1106 86-87 0.7550076936748376 1106 88-89 1.1021467331668937 1106 90-91 1.1102024052218695 1106 92-93 1.0700688437916739 1106 94-95 1.0692355075451196 1106 96-97 0.867137980348506 1106 98-99 1.0792264068779502 1106 100-101 1.2303350316898545 1107 1 0.19560660390147433 1107 2 0.34222753278721996 1107 3 0.33098754894323434 1107 4 0.19488023104679542 1107 5 0.270434530118294 1107 6 0.16920130861630867 1107 7 0.2827636490220513 1107 8 0.15881239934404334 1107 9 0.1905337461588701 1107 10-11 -0.0443589036876304 1107 12-13 0.12036656456967876 1107 14-15 0.38354158778023617 1107 16-17 0.4291268112385822 1107 18-19 0.24760017258959266 1107 20-21 0.4160525363194978 1107 22-23 0.46382630102174005 1107 24-25 0.48272616961097015 1107 26-27 0.6119872130477759 1107 28-29 0.7432529428777244 1107 30-31 -0.033864696832559105 1107 32-33 0.3553388201568737 1107 34-35 0.006940849110641523 1107 36-37 0.053031722814452564 1107 38-39 0.10047731336299393 1107 40-41 0.28657451529898026 1107 42-43 -0.06720113176917408 1107 44-45 0.30292882190413906 1107 46-47 0.5541257040555365 1107 48-49 0.37433783173047885 1107 50-51 0.297406410705527 1107 52-53 0.17300765391611606 1107 54-55 0.29092070744444953 1107 56-57 0.3574573686071929 1107 58-59 0.3769535933904251 1107 60-61 0.34657103150096447 1107 62-63 0.33529242688334904 1107 64-65 0.21558982242000013 1107 66-67 0.3456415434727944 1107 68-69 0.8753234186533909 1107 70-71 0.5626604513323237 1107 72-73 0.6577477773337606 1107 74-75 0.7106033740727682 1107 76-77 0.4509802340434419 1107 78-79 0.6386681514197825 1107 80-81 0.5660239687238686 1107 82-83 0.5577275053583577 1107 84-85 0.5664949437647238 1107 86-87 0.7557302662352718 1107 88-89 0.492229803746568 1107 90-91 0.3782515603678007 1107 92-93 0.522187042514286 1107 94-95 0.42574674531696743 1107 96-97 0.5533545297866169 1107 98-99 0.4830282501959253 1107 100-101 0.9529833864477162 1108 1 -0.09105123893045786 1108 2 0.02076744766316807 1108 3 0.14538856627947538 1108 4 0.14615449659231672 1108 5 0.08164339547695221 1108 6 0.09635276714428898 1108 7 -0.23763498068831979 1108 8 -0.1436634648727093 1108 9 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2316 66-67 -0.9310397430769086 2316 68-69 -0.9566291479482985 2316 70-71 -0.7637284375565621 2316 72-73 -1.1011865956356992 2316 74-75 -1.3318348975321719 2316 76-77 -1.3685860427596772 2316 78-79 -1.0620303541032854 2316 80-81 -1.2588217102884762 2316 82-83 -0.8452127155643225 2316 84-85 -1.2456395591592546 2316 86-87 -1.6528548539726557 2316 88-89 -1.5240551215295852 2316 90-91 -1.2097114025951647 2316 92-93 -0.8398987274662204 2316 94-95 -0.8099176471456708 2316 96-97 -0.5945093428579575 2316 98-99 -0.41203347819913816 2316 100-101 -0.12502993389016837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3573.0 3 891.0 4 771.0 5 860.0 6 949.0 7 1052.0 8 1295.0 9 1723.0 10 2043.0 11 2660.0 12 3195.0 13 3994.0 14 4638.0 15 5455.0 16 6598.0 17 8614.0 18 7671.0 19 5752.0 20 5606.0 21 5830.0 22 6382.0 23 6954.0 24 7679.0 25 8730.0 26 9929.0 27 11604.0 28 13434.0 29 15941.0 30 19603.0 31 23717.0 32 29745.0 33 37870.0 34 50037.0 35 69500.0 36 96900.0 37 126874.0 38 95446.0 39 11357.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43811394891945 19.06258770698849 15.199550940218916 22.29974740387314 2 14.955891614988296 20.166277880721502 41.55039325125578 23.327437253034425 3 18.857777665564168 24.67815734031302 30.66124674931199 25.802818244810823 4 12.384305693885281 17.17073239160015 37.897092036416176 32.54786987809839 5 13.261797577847389 37.250451365459426 34.80346839459793 14.68428266209526 6 30.72614343445954 36.68550138141489 17.963019292149273 14.625335891976299 7 27.30804755904585 33.32300822317936 21.305561123016915 18.06338309475788 8 25.44311355424304 35.566537813571344 20.180272862080468 18.810075770105147 9 25.53974986893491 17.19630046005949 21.056062215967234 36.20788745503837 10-11 24.334538134877498 27.106352647304337 27.672868696256142 20.886240521562023 12-13 25.30736053309934 24.58281528371364 28.378232524498433 21.731591658688583 14-15 22.270947412538206 26.007710837293764 26.964101363658983 24.75724038650905 16-17 21.215344715101903 29.284123056525523 27.662210524176622 21.838321704195952 18-19 21.095878123013676 27.32876553929473 30.636947272743836 20.938409064947752 20-21 21.913493522209617 26.514620137102547 30.81955312390732 20.75233321678052 22-23 21.581936740750603 26.370704667533296 30.765915549217404 21.281443042498697 24-25 21.32082677813712 26.63714054457475 30.908523915808505 21.13350876147962 26-27 21.10658724597057 26.81366503153469 30.777925718290117 21.301822004204627 28-29 20.98015703089246 26.881816136630754 31.005296270884248 21.13273056159254 30-31 21.133542690588804 26.70412297367663 30.87874682792922 21.283587507805343 32-33 20.91831621235388 26.863408996451515 30.74669524329499 21.471579547899612 34-35 21.376023660262184 26.851594229306908 30.61172570635624 21.16065640407467 36-37 21.353962283190008 26.709253175768854 30.531317621733134 21.405466919308004 38-39 21.06272881741903 26.9631965246028 30.653914201131606 21.32016045684657 40-41 21.340023219819663 26.951876678514804 30.483825650964214 21.224274450701326 42-43 21.21142259713286 26.960350817691687 30.343726218689255 21.484500366486202 44-45 21.282137526540392 27.13686084074362 30.142459725171157 21.43854190754483 46-47 21.3765914701614 27.36813633054228 29.697959652740842 21.557312546555472 48-49 21.312053307991295 27.545872877709236 29.55319235421669 21.58888146008278 50-51 21.481682378697794 27.7388130618698 29.285488026058704 21.4940165333737 52-53 21.56446024537392 27.810609411350733 29.109466375165688 21.515463968109664 54-55 21.4959931383019 27.705647726922354 29.121153258605855 21.677205876169893 56-57 21.405019016095718 27.99471977533821 29.004730901926273 21.595530306639798 58-59 21.539990106349734 27.67958867078949 29.14226536563764 21.638155857223136 60-61 21.397710323162364 27.56068358224222 29.509872401064058 21.531733693531358 62-63 21.376918975462758 27.693081634097243 29.489391904342522 21.440607486097477 64-65 21.354433785385783 27.588721949907097 29.34844752352046 21.708396741186657 66-67 21.24887199923772 27.819581532735842 29.1773161148571 21.754230353169334 68-69 21.178557715752685 27.815511940562036 29.24554873896118 21.760381604724095 70-71 21.226111358293718 27.76144815529763 29.17232753758246 21.840112948826192 72-73 21.341389931307788 27.69801210171268 29.100863487187823 21.859734479791708 74-75 21.290488042783625 28.025251421075648 28.851269157893555 21.832991378247172 76-77 21.33598280273878 27.808778562840352 28.83715292277768 22.018085711643185 78-79 21.403005990961006 27.825386259328035 28.772098237746558 21.999509511964405 80-81 21.32437486818402 28.078538151135913 28.716509982279725 21.880576998400343 82-83 21.36680164212355 28.00219977301705 28.73218814364378 21.89881044121562 84-85 21.342992691729997 27.82670040576591 28.76573463806129 22.0645722644428 86-87 21.47159276008589 27.748403526778365 28.831237980474512 21.948765732661233 88-89 21.36414526183927 27.64932468125712 28.72048852189339 22.266041535010224 90-91 21.306456121814836 27.72467369775118 28.86544415570585 22.103426024728137 92-93 21.44931496291937 27.678580811955484 28.761328246788505 22.110775978336644 94-95 21.316972417929186 27.728220477207376 28.67938434979514 22.2754227550683 96-97 21.268247567866762 27.673805128061407 28.623466889006444 22.434480415065387 98-99 21.323534048637207 27.749637571741175 28.52445278412869 22.402375595492927 100-101 21.692236123624554 27.337997441419574 28.788303392150794 22.181463042805078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1101.0 1 687.0 2 315.5 3 365.0 4 312.0 5 284.5 6 430.0 7 864.5 8 1453.0 9 1738.0 10 1540.5 11 1271.0 12 1250.0 13 1372.5 14 1468.0 15 1473.0 16 1516.5 17 1582.0 18 1596.0 19 1674.5 20 1863.5 21 2176.0 22 2533.5 23 3019.0 24 3660.5 25 4424.0 26 5459.5 27 6658.0 28 7607.5 29 8554.0 30 9965.5 31 11132.0 32 12198.5 33 13388.0 34 14626.5 35 16021.0 36 17584.5 37 19352.0 38 20478.0 39 21040.5 40 21593.5 41 22894.5 42 24368.0 43 25552.0 44 26461.5 45 26829.5 46 26910.0 47 27082.0 48 27765.0 49 27656.5 50 26455.0 51 24946.0 52 23395.5 53 22283.5 54 21101.5 55 19122.5 56 16917.5 57 14771.0 58 13052.5 59 11453.5 60 9581.0 61 7717.5 62 5944.5 63 4471.5 64 3345.0 65 2474.0 66 1814.0 67 1439.0 68 1090.5 69 727.0 70 522.5 71 414.5 72 323.5 73 243.5 74 185.0 75 141.0 76 100.5 77 69.0 78 49.0 79 34.0 80 22.5 81 18.5 82 12.5 83 7.0 84 5.0 85 2.5 86 4.0 87 5.5 88 5.5 89 3.0 90 2.0 91 2.0 92 0.5 93 1.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31879520869965405 2 0.2764104135105382 3 0.27319308582291546 4 0.2810265793232142 5 0.2856427451358901 6 0.20702804250789295 7 0.196956408007509 8 0.19653675656999295 9 0.20884653207046228 10-11 0.20751763585166158 12-13 0.2050696691328183 14-15 0.20527949485157626 16-17 0.19157088122605362 18-19 0.19660669847624562 20-21 0.185415993475819 22-23 0.1939489060386443 24-25 0.1939489060386443 26-27 0.19289977744485431 28-29 0.20318123766399626 30-31 0.19940437472635228 32-33 0.20639856535161893 34-35 0.20234193478896428 36-37 0.18898303069470496 38-39 0.18065994385063766 40-41 0.17688308091299368 42-43 0.1882836116321783 44-45 0.18800384400716766 46-47 0.18863332116344164 48-49 0.20143269000767963 50-51 0.19765582707003565 52-53 0.2181488056020669 54-55 0.18926279831971565 56-57 0.178072093319289 58-59 0.18045011813187967 60-61 0.16751086547513636 62-63 0.17478482372541368 64-65 0.17415534656913967 66-67 0.17163743794404368 68-69 0.17639348756922502 70-71 0.17926110572558432 72-73 0.17639348756922502 74-75 0.18674488969461964 76-77 0.17863162856931034 78-79 0.18240849150695432 80-81 0.180380176225627 82-83 0.16352417681873435 84-85 0.16324440919372368 86-87 0.167370981662631 88-89 0.17009871600648502 90-91 0.173246101787855 92-93 0.1722669151003177 94-95 0.17163743794404368 96-97 0.175764010412951 98-99 0.180380176225627 100-101 0.16814034263141037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 714879.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.90470389570578 #Duplication Level Percentage of deduplicated Percentage of total 1 85.7232634493461 59.92459348400118 2 8.57029022846834 11.982072014426798 3 2.3243920040795754 4.874578043481866 4 1.0503745766739605 2.9370449504788203 5 0.566861984254279 1.9813159579513802 6 0.37830146069819787 1.5867030956052302 7 0.2463149642541932 1.205300224888951 8 0.1987481918307327 1.1114746799787438 9 0.14494275696459336 0.9118962456693431 >10 0.7574521928960112 9.275557180867988 >50 0.027441044196147665 1.3251331947229024 >100 0.010415372580845236 1.1768350226685058 >500 6.00886878517038E-4 0.33435836176768585 >1k 6.00886878517038E-4 1.3731375434906596 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3999 0.5593953662088269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3493 0.4886141570811284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 0.32383102594984603 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 941 0.13163066756751843 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 901 0.1260353150673051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3988381250533308E-4 0.0 18-19 0.0 0.0 0.0 1.3988381250533308E-4 0.0 20-21 0.0 0.0 0.0 1.3988381250533308E-4 0.0 22-23 0.0 0.0 0.0 4.1965143751599923E-4 0.0 24-25 0.0 0.0 0.0 6.294771562739988E-4 0.0 26-27 0.0 0.0 0.0 9.791866875373315E-4 0.0 28-29 0.0 0.0 0.0 0.0023080829063379955 0.0 30-31 0.0 0.0 0.0 0.004756049625181325 0.0 32-33 0.0 0.0 0.0 0.008043319219056651 0.0 34-35 0.0 0.0 0.0 0.012030007875458645 0.0 36-37 0.0 0.0 0.0 0.015666987000597304 0.0 38-39 0.0 0.0 0.0 0.020213210907020628 0.0 40-41 0.0 0.0 0.0 0.02545885387597062 0.0 42-43 6.994190625266654E-5 0.0 0.0 0.0363697912513866 0.0 44-45 1.3988381250533308E-4 0.0 0.0 0.05021828868941457 0.0 46-47 1.3988381250533308E-4 0.0 0.0 0.0645563794712112 0.0 48-49 1.3988381250533308E-4 0.0 0.0 0.08379040369069451 0.0 50-51 1.3988381250533308E-4 0.0 0.0 0.1082700708791278 0.0 52-53 1.3988381250533308E-4 0.0 0.0 0.14680806122434706 0.0 54-55 1.3988381250533308E-4 0.0 0.0 0.183527562006997 0.0 56-57 2.7976762501066615E-4 0.0 0.0 0.24046027369666756 0.0 58-59 2.7976762501066615E-4 0.0 0.0 0.30739467798046943 0.0 60-61 2.7976762501066615E-4 0.0 0.0 0.3857296129834559 0.0 62-63 2.7976762501066615E-4 0.0 0.0 0.47448589201808977 0.0 64-65 2.7976762501066615E-4 0.0 0.0 0.5501630345834749 0.0 66-67 4.1965143751599923E-4 0.0 0.0 0.667595495181702 0.0 68-69 4.1965143751599923E-4 0.0 0.0 0.8111862287184265 0.0 70-71 4.1965143751599923E-4 0.0 0.0 0.9389700914420482 0.0 72-73 4.1965143751599923E-4 0.0 0.0 1.11501386948001 0.0 74-75 4.1965143751599923E-4 0.0 0.0 1.2938553237680783 0.0 76-77 4.1965143751599923E-4 0.0 0.0 1.446398621305144 0.0 78-79 4.1965143751599923E-4 0.0 0.0 1.623421586030643 0.0 80-81 4.1965143751599923E-4 0.0 0.0 1.8713656436963457 0.0 82-83 4.1965143751599923E-4 0.0 0.0 2.1039924238927146 0.0 84-85 4.1965143751599923E-4 0.0 0.0 2.4069807617792662 0.0 86-87 4.1965143751599923E-4 0.0 0.0 2.739624467916948 0.0 88-89 4.1965143751599923E-4 0.0 0.0 3.1732642866834806 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 51.269886 1 GTATCAA 3090 0.0 39.242615 1 TATCAAC 4010 0.0 29.991627 2 ATCAACG 4065 0.0 29.581684 3 TCAACGC 4085 0.0 29.443052 4 CAACGCA 4140 0.0 29.051897 5 AACGCAG 4215 0.0 28.402246 6 GTACCGA 35 0.008392689 27.142555 10-11 CCGTCCG 90 8.933802E-4 26.394148 9 GTACATG 2490 0.0 25.78165 1 TACATGG 2485 0.0 25.059292 2 CGCAGAG 4790 0.0 24.895357 8 ACGCAGA 4805 0.0 24.817642 7 GTCTAGG 175 1.4686339E-7 24.455736 1 ACATGGG 2495 0.0 24.383854 3 CTAAGAC 215 1.6480044E-9 24.317402 3 GCAGAGT 5050 0.0 23.42546 9 GAGTACT 3210 0.0 23.381443 12-13 CGGAGTA 115 3.6288839E-9 22.71714 14-15 CAATACT 300 7.2759576E-12 22.185085 4 >>END_MODULE