FastQCFastQC Report
Thu 26 May 2016
SRR937882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714879
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49690.695082664339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.6633290389002894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.4396548227042618No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.17457499800665569No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.12085961400460778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.052.693731
GTATCAA38500.036.275931
ATCAACG49350.028.0096323
TCAACGC49450.027.9529914
TATCAAC49950.027.8633752
TGGTATC10950.027.763072
CAACGCA50100.027.5903285
AACGCAG51500.026.9306496
GTGGTAT13150.024.203661
GAGTACT41900.023.86547312-13
GACACCG1401.7770442E-523.75217
CGCAGAG59500.023.3928248
ACGCAGA59300.023.3916117
GTACTTT44150.022.53847514-15
GCAGAGT62350.022.0949769
GTATAGG1754.042604E-621.7162041
CAGAGTA60350.021.33162710-11
GTACATG25200.021.1129761
AGTACTT43900.021.10095412-13
TCGGATA804.9923616E-520.7801854-55
Produced by FastQC (version 0.11.5)