##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714879 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06387654414244 31.0 31.0 34.0 30.0 34.0 2 32.29633126724942 34.0 31.0 34.0 31.0 34.0 3 32.35537762334605 34.0 31.0 34.0 30.0 34.0 4 36.018943065889474 37.0 35.0 37.0 35.0 37.0 5 35.91618301838493 37.0 35.0 37.0 35.0 37.0 6 35.95166594626503 37.0 35.0 37.0 35.0 37.0 7 35.89356240706469 37.0 35.0 37.0 35.0 37.0 8 35.87584052685839 37.0 35.0 37.0 35.0 37.0 9 37.53298110589345 39.0 37.0 39.0 35.0 39.0 10-11 37.44908019399087 39.0 37.0 39.0 34.0 39.0 12-13 37.32918717713068 39.0 37.0 39.0 34.0 39.0 14-15 38.49307435244286 40.0 38.0 41.0 34.0 41.0 16-17 38.44864025940054 40.0 38.0 41.0 34.0 41.0 18-19 38.44528934267198 40.0 38.0 41.0 34.0 41.0 20-21 38.35037048227742 40.0 38.0 41.0 34.0 41.0 22-23 38.18383880348982 40.0 37.5 41.0 33.5 41.0 24-25 38.24689982500535 40.0 38.0 41.0 34.0 41.0 26-27 38.091840717100375 40.0 37.5 41.0 33.0 41.0 28-29 37.93611646166694 40.0 37.0 41.0 33.0 41.0 30-31 37.964195339351136 40.0 37.0 41.0 33.0 41.0 32-33 37.84823795355578 40.0 37.0 41.0 33.0 41.0 34-35 37.69361038721238 40.0 37.0 41.0 33.0 41.0 36-37 37.513220419119875 40.0 36.0 41.0 33.0 41.0 38-39 37.35746399040956 40.0 36.0 41.0 32.5 41.0 40-41 37.162115546826804 40.0 36.0 41.0 31.5 41.0 42-43 36.913311203714194 39.0 36.0 41.0 31.0 41.0 44-45 36.573824381468754 39.0 35.0 41.0 30.5 41.0 46-47 36.16950840631772 39.0 35.0 41.0 30.0 41.0 48-49 35.7951464513575 39.0 35.0 40.0 28.5 41.0 50-51 35.518621333120706 39.0 35.0 40.0 27.5 41.0 52-53 35.241882192650785 39.0 35.0 40.0 27.0 41.0 54-55 35.001730362760696 38.0 34.5 40.0 26.0 41.0 56-57 34.72132416814594 38.0 34.0 40.0 26.0 41.0 58-59 34.44686093730547 38.0 34.0 40.0 25.0 41.0 60-61 34.06717640327943 37.5 33.5 40.0 23.5 41.0 62-63 33.69900080992727 37.0 33.0 40.0 23.0 41.0 64-65 33.297479713350086 36.5 32.5 39.0 22.0 41.0 66-67 32.844091097934054 36.0 32.0 39.0 21.0 40.0 68-69 32.38290395997085 35.5 31.5 39.0 19.5 40.0 70-71 31.935665336371606 35.0 31.0 38.0 18.0 40.0 72-73 31.40479787488512 35.0 31.0 37.5 15.5 39.5 74-75 30.868453962139046 34.5 30.0 37.0 12.0 39.0 76-77 29.647408862199057 33.5 28.5 35.5 10.0 38.0 78-79 30.25886408748893 34.0 30.0 36.0 7.5 38.0 80-81 30.038404401304277 34.0 30.0 35.5 7.0 37.5 82-83 29.641965283635415 34.0 29.0 35.0 2.0 37.0 84-85 29.22453030512856 34.0 29.0 35.0 2.0 36.5 86-87 28.73975176218633 34.0 29.0 35.0 2.0 36.0 88-89 28.307972398126118 33.5 28.5 35.0 2.0 36.0 90-91 27.70883044543202 33.0 26.5 35.0 2.0 35.0 92-93 27.140244712741598 33.0 26.0 35.0 2.0 35.0 94-95 26.579583398029598 32.0 25.0 34.5 2.0 35.0 96-97 25.91799940969031 32.0 24.5 34.0 2.0 35.0 98-99 24.90156236230187 32.0 19.0 34.0 2.0 35.0 100-101 22.217424906872353 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23469059631881706 1101 2 0.11632028454643972 1101 3 0.1630600492610199 1101 4 0.12074329416883955 1101 5 0.09466223343768121 1101 6 0.11643780316685337 1101 7 0.14001495135314457 1101 8 0.15383734524144188 1101 9 0.23939687908145402 1101 10-11 0.2589733024797525 1101 12-13 0.2632903035804688 1101 14-15 0.2620862884947499 1101 16-17 0.239622296752529 1101 18-19 0.20535008908045427 1101 20-21 0.2149459827587208 1101 22-23 0.26330780797935915 1101 24-25 0.20842412580653757 1101 26-27 0.11551293288001574 1101 28-29 0.2938514591985708 1101 30-31 0.4113939509842979 1101 32-33 0.34648096549794616 1101 34-35 0.1952091978813968 1101 36-37 0.21416482697742367 1101 38-39 0.01982617641574791 1101 40-41 0.3538459720472531 1101 42-43 0.43802366269279247 1101 44-45 0.2636531190371443 1101 46-47 0.4575035798095186 1101 48-49 0.5185216612805803 1101 50-51 0.5371105876589439 1101 52-53 0.33086180017301103 1101 54-55 0.5859629087025695 1101 56-57 0.6044983041932639 1101 58-59 0.3277620588354111 1101 60-61 0.4293360316665229 1101 62-63 0.566557192731608 1101 64-65 0.511583502016677 1101 66-67 0.35077347473223597 1101 68-69 0.3194811964341966 1101 70-71 0.4714648108650188 1101 72-73 0.2809231275363686 1101 74-75 0.1925966716939378 1101 76-77 0.15013861139018658 1101 78-79 0.13325885050754493 1101 80-81 0.051628631736679864 1101 82-83 0.3059620972553283 1101 84-85 0.14968261649120862 1101 86-87 0.04420773906647213 1101 88-89 0.37911810301315896 1101 90-91 0.2795126475838643 1101 92-93 0.465922623672423 1101 94-95 0.3143847008674605 1101 96-97 0.4301463454017913 1101 98-99 0.36563732607009314 1101 100-101 0.8383566602336288 1102 1 0.329558228686448 1102 2 0.28265395088011047 1102 3 0.28227833097930244 1102 4 0.11883270475825469 1102 5 0.10045643923188408 1102 6 0.18971452644357356 1102 7 0.22617878751697873 1102 8 0.24316052206462047 1102 9 0.3447290469136277 1102 10-11 0.15214887690532208 1102 12-13 0.22894340048356554 1102 14-15 0.3311484763069359 1102 16-17 0.33187379525403315 1102 18-19 0.25951467449503696 1102 20-21 0.2591392894520226 1102 22-23 0.2881454703170263 1102 24-25 0.08578426566668185 1102 26-27 0.2767766691437572 1102 28-29 0.2607096010567105 1102 30-31 0.29948086407121366 1102 32-33 0.18412457189155162 1102 34-35 0.1742239331461306 1102 36-37 0.22660238941499244 1102 38-39 0.3547787238682929 1102 40-41 0.32117863937991586 1102 42-43 0.3953163699855011 1102 44-45 0.3402390331230549 1102 46-47 0.39421686652280385 1102 48-49 0.4410304025393188 1102 50-51 0.3407819163302719 1102 52-53 0.17584182015303185 1102 54-55 0.3384728987125598 1102 56-57 0.23707198051695144 1102 58-59 0.11408198890534749 1102 60-61 0.3019696480501395 1102 62-63 0.22843281710722607 1102 64-65 0.18796337214654812 1102 66-67 0.3478888593476199 1102 68-69 0.31923768994069235 1102 70-71 0.3024962793964896 1102 72-73 0.3912190815823209 1102 74-75 0.2289478205450841 1102 76-77 0.21962537462695408 1102 78-79 0.46563897038766555 1102 80-81 0.5763289314369793 1102 82-83 0.48891039895363164 1102 84-85 0.3411161829247753 1102 86-87 0.6312394573481903 1102 88-89 0.5286061150011712 1102 90-91 0.4503730371942538 1102 92-93 0.5389620842118852 1102 94-95 0.3271906449234052 1102 96-97 0.24252771403315876 1102 98-99 0.07421624714901043 1102 100-101 -0.013116866239894165 1103 1 0.2262239644479287 1103 2 0.15634310648415806 1103 3 0.19896827027562836 1103 4 -0.017900489155792343 1103 5 0.030872359009507022 1103 6 0.10995760094196783 1103 7 0.12784922110230923 1103 8 0.057302595515203336 1103 9 0.12876932696454446 1103 10-11 0.12582239113734772 1103 12-13 0.050590355774140505 1103 14-15 0.07418319474059132 1103 16-17 0.11826434028818511 1103 18-19 0.0689570298150528 1103 20-21 0.06513377762736638 1103 22-23 0.108455331850287 1103 24-25 0.1161247388263078 1103 26-27 -0.05704701308417981 1103 28-29 -0.030668052023067105 1103 30-31 0.1440694905747293 1103 32-33 0.15227164841628849 1103 34-35 0.09309202821103923 1103 36-37 0.061988043949583016 1103 38-39 0.14553667553050076 1103 40-41 0.09907332152991444 1103 42-43 0.17563790030703075 1103 44-45 0.2599565791703924 1103 46-47 0.23418452297981673 1103 48-49 0.4366781501817485 1103 50-51 0.7418266950346322 1103 52-53 0.5973383693091563 1103 54-55 0.42485819387444934 1103 56-57 0.28596750481140276 1103 58-59 0.22669797319685614 1103 60-61 0.1446750858353667 1103 62-63 0.3201524154757607 1103 64-65 0.6100230648179448 1103 66-67 0.4537116712874578 1103 68-69 0.4537256163169161 1103 70-71 0.5861696096123659 1103 72-73 0.34174282399435896 1103 74-75 0.2344536917424449 1103 76-77 0.5173410307202673 1103 78-79 0.5270608522882689 1103 80-81 0.4973643577915503 1103 82-83 0.5713239221916204 1103 84-85 0.6037483498122604 1103 86-87 0.6121805267201097 1103 88-89 0.768770452239977 1103 90-91 0.6699814183717869 1103 92-93 0.6972598050096046 1103 94-95 0.5830713137030088 1103 96-97 0.5375724299555351 1103 98-99 0.6703695520789168 1103 100-101 1.236893958239012 1104 1 0.05658814321636285 1104 2 0.09431451254067014 1104 3 -0.03930286560188989 1104 4 -0.12490966398411274 1104 5 0.07557853935398384 1104 6 -0.06043809870904937 1104 7 0.1207148070529982 1104 8 0.0688625977666959 1104 9 -0.05957498239040149 1104 10-11 0.01932683783328315 1104 12-13 0.15823979852995507 1104 14-15 0.052111541020003926 1104 16-17 0.08652886240908941 1104 18-19 0.09784648157684472 1104 20-21 -0.09968244436970508 1104 22-23 -0.12604103761948693 1104 24-25 0.007792812675226912 1104 26-27 0.10797325034033634 1104 28-29 -0.15004284094573705 1104 30-31 0.05112158446193149 1104 32-33 0.035658454675434825 1104 34-35 0.18792204059423767 1104 36-37 0.06058437964698271 1104 38-39 0.04037090946047783 1104 40-41 -0.09232105536977997 1104 42-43 0.027489752158885494 1104 44-45 -0.014232883848862343 1104 46-47 0.21320343550937082 1104 48-49 0.34682252708145 1104 50-51 0.4922242240225856 1104 52-53 0.42114859670980564 1104 54-55 0.4095199072595719 1104 56-57 0.498022835217796 1104 58-59 0.4616094614328148 1104 60-61 0.4316628714933657 1104 62-63 0.6272804972049073 1104 64-65 0.2856816261148012 1104 66-67 0.12894807790683416 1104 68-69 0.09503134073434438 1104 70-71 -0.03526318336297507 1104 72-73 0.009007543120780781 1104 74-75 -0.11010895601169324 1104 76-77 -0.049338300586722283 1104 78-79 0.2727970900457848 1104 80-81 0.2554345480425937 1104 82-83 0.4840645503577825 1104 84-85 0.1737627030712936 1104 86-87 0.20827700313573771 1104 88-89 0.3370997047447588 1104 90-91 0.22071033878155433 1104 92-93 0.3849251489249461 1104 94-95 0.555708654691415 1104 96-97 0.7070042097596563 1104 98-99 0.6932257099084751 1104 100-101 1.0084414365684076 1105 1 0.0818800607496577 1105 2 0.05618278475377281 1105 3 0.13579063923299373 1105 4 -0.04268242442928738 1105 5 -0.012794932640176171 1105 6 -0.06976577614017287 1105 7 -0.14855631135605307 1105 8 -0.014162853793237673 1105 9 0.01581043997777698 1105 10-11 0.03966984093491277 1105 12-13 0.035223925514088705 1105 14-15 -0.09217098041769844 1105 16-17 0.055005189721633485 1105 18-19 0.07743506030335112 1105 20-21 -0.13166793971727486 1105 22-23 -0.23749994110425376 1105 24-25 0.02620870393594288 1105 26-27 -0.2052223344069759 1105 28-29 0.0408381570473324 1105 30-31 0.11798324291842022 1105 32-33 0.0119629611523564 1105 34-35 0.17065785522660093 1105 36-37 0.15808597607099273 1105 38-39 0.11555107731305725 1105 40-41 0.15689783716808137 1105 42-43 0.15648081218443366 1105 44-45 0.11519149126517902 1105 46-47 0.3931204214608428 1105 48-49 0.402480709894796 1105 50-51 0.5531905166699076 1105 52-53 0.611767325044049 1105 54-55 0.41257591893626966 1105 56-57 0.1362161393593837 1105 58-59 0.34755066741712426 1105 60-61 0.6027197041881323 1105 62-63 0.37602046253969945 1105 64-65 0.2860237169741353 1105 66-67 0.4723388825045376 1105 68-69 0.14298677769839685 1105 70-71 0.26651796971128405 1105 72-73 0.5380282510207977 1105 74-75 0.2848463441160227 1105 76-77 -0.1568118455602665 1105 78-79 0.25936432230267314 1105 80-81 0.271432997230697 1105 82-83 0.3502158001297211 1105 84-85 0.14497005574416377 1105 86-87 0.3438590137177471 1105 88-89 0.3458743352866307 1105 90-91 0.1451994830982848 1105 92-93 0.1819341345115184 1105 94-95 0.47354983330845357 1105 96-97 0.6162931168933881 1105 98-99 0.42117795402451463 1105 100-101 -0.005327720519716195 1106 1 0.06458865524912838 1106 2 0.15985470711312644 1106 3 0.11389093880158896 1106 4 0.020603648867904667 1106 5 0.011894463573135283 1106 6 0.010670452238208838 1106 7 0.0193963226699978 1106 8 -0.05533735103002613 1106 9 -0.07253581188349045 1106 10-11 -0.15553235315494618 1106 12-13 -0.14961990449103268 1106 14-15 0.051183481624200056 1106 16-17 0.034797551605201704 1106 18-19 0.06027607707095228 1106 20-21 0.07988104769378168 1106 22-23 -0.007073423692197878 1106 24-25 0.150617953484236 1106 26-27 0.14707975315651822 1106 28-29 0.11005380523962316 1106 30-31 0.1474957524086804 1106 32-33 0.15067381565853566 1106 34-35 0.08391538416822897 1106 36-37 0.16486305348210806 1106 38-39 0.0020004537513145237 1106 40-41 0.1543347306005245 1106 42-43 -0.02985791809201288 1106 44-45 0.1754112533759269 1106 46-47 0.34690799260102523 1106 48-49 0.4300598542703895 1106 50-51 0.5728213822407042 1106 52-53 0.45470274417524337 1106 54-55 0.7066269221085193 1106 56-57 0.41580957356905657 1106 58-59 0.5307818884681481 1106 60-61 0.5172043461477429 1106 62-63 0.6332168771332221 1106 64-65 0.4067133721465481 1106 66-67 0.22681034109168507 1106 68-69 0.2154070447794041 1106 70-71 0.4271344100830028 1106 72-73 0.3218915676983549 1106 74-75 0.3157743005167255 1106 76-77 -0.047578187939514294 1106 78-79 0.06612786321526443 1106 80-81 0.2373533908485328 1106 82-83 0.1642360813357655 1106 84-85 0.4058095540697586 1106 86-87 0.30639528270562977 1106 88-89 0.17470531150359392 1106 90-91 0.1600984596212882 1106 92-93 0.5278622969024163 1106 94-95 0.6999418856181947 1106 96-97 0.7709122998774198 1106 98-99 0.9587947063242481 1106 100-101 0.7748424862355847 1107 1 0.25009273803674503 1107 2 0.14517081997592385 1107 3 0.23821384283586866 1107 4 -0.0848616272255498 1107 5 0.26835214002232277 1107 6 0.09474151699688349 1107 7 0.20634555057847592 1107 8 0.04635842065725626 1107 9 0.1110077243765133 1107 10-11 0.03446885955425216 1107 12-13 0.10284088457841989 1107 14-15 0.18468255188047777 1107 16-17 0.06744469365387573 1107 18-19 0.1783080521699958 1107 20-21 0.30497503265618775 1107 22-23 0.042844718437322626 1107 24-25 0.2051090198598544 1107 26-27 -0.03561488666885282 1107 28-29 -0.09816836308441168 1107 30-31 -0.20689324427657851 1107 32-33 -0.23313853780577176 1107 34-35 -0.06103368204569648 1107 36-37 0.018003970290251914 1107 38-39 0.03650708322822993 1107 40-41 0.31058725707801216 1107 42-43 0.5786593428021618 1107 44-45 0.4714083024502216 1107 46-47 0.2610835524684276 1107 48-49 0.3355443315927218 1107 50-51 0.3367574320426314 1107 52-53 0.3256755390656991 1107 54-55 0.4844990217123666 1107 56-57 0.3153265110741117 1107 58-59 0.1347767546659 1107 60-61 0.4744260293880984 1107 62-63 0.6153496283661468 1107 64-65 0.533906354602685 1107 66-67 0.5690065574390033 1107 68-69 0.3807195195146278 1107 70-71 0.3930471758684355 1107 72-73 0.7320651872310577 1107 74-75 0.7023910439883103 1107 76-77 0.5359269458627338 1107 78-79 0.5662390282245653 1107 80-81 0.6570114068562951 1107 82-83 0.6918757450110853 1107 84-85 0.8938441567053808 1107 86-87 0.9554574542635592 1107 88-89 1.1946341658976394 1107 90-91 0.9418360697757073 1107 92-93 1.2318601948731533 1107 94-95 1.5377470840982639 1107 96-97 1.5042577603026785 1107 98-99 1.4562530699753111 1107 100-101 1.4008025476795147 1108 1 -0.052045744263679694 1108 2 -0.07484111815342231 1108 3 -0.15395370621427418 1108 4 0.04080016037955403 1108 5 -0.10340380308989694 1108 6 -0.2585103256274337 1108 7 -0.2615642386875834 1108 8 -0.19965787748454744 1108 9 0.018175792475751962 1108 10-11 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0.6818277062441709 2316 94-95 0.24563369878312358 2316 96-97 0.9205183191070994 2316 98-99 1.1109395547056522 2316 100-101 0.9393827945930973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 15.0 6 50.0 7 85.0 8 220.0 9 445.0 10 799.0 11 1259.0 12 1873.0 13 2613.0 14 3375.0 15 4673.0 16 6249.0 17 8699.0 18 8122.0 19 5320.0 20 4470.0 21 4509.0 22 4761.0 23 5302.0 24 6085.0 25 6897.0 26 8636.0 27 10493.0 28 12722.0 29 15555.0 30 19082.0 31 23711.0 32 29480.0 33 37822.0 34 50665.0 35 71852.0 36 103618.0 37 145045.0 38 102328.0 39 8042.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33044972290197 20.122731832624673 15.882103566526615 21.664714877946746 2 15.67426095884758 21.047198197177426 40.14833279478066 23.13020804919434 3 19.05441340422645 24.91750352157498 31.059521961059144 24.968561113139426 4 12.913653919054832 18.11719186044072 37.166569447416975 31.80258477308747 5 13.229685222029142 37.65428989570829 34.60710817851876 14.508916703743813 6 30.69764253810785 37.31204861242252 17.358741829036802 14.63156702043283 7 26.999813954529365 33.92895860698104 20.98564931967508 18.085578118814514 8 25.803947241421277 36.029034284123604 19.516869288369083 18.650149186086036 9 25.584189771975396 17.193539046468004 20.765751966416694 36.456519215139906 10-11 24.540516646873105 27.484231597235336 27.140327244190978 20.83492451170058 12-13 25.490331853996835 24.90538191712218 28.275261506824883 21.329024722056104 14-15 22.35119509735214 26.244021715563054 26.97127765677828 24.433505530306526 16-17 21.085036768460117 29.450578349622802 27.830933626529802 21.633451255387275 18-19 20.95809220861153 27.24747824457006 31.125337294842904 20.66909225197551 20-21 21.67156959429498 26.464828313602723 31.50225422763852 20.361347864463777 22-23 21.443084526379916 26.302349443221612 31.46299719599956 20.79156883439891 24-25 21.312208079968777 26.537707779918 31.553941296359245 20.596142843753977 26-27 21.102470918033337 26.744148635567566 31.40537659678042 20.748003849618676 28-29 20.886116675432806 26.890408352824004 31.512494544477892 20.7109804272653 30-31 21.10636904986718 26.507213108791838 31.593878124829516 20.792539716511467 32-33 20.924939745047762 26.71892725901866 31.429934296573265 20.92619869936031 34-35 21.342562867282435 26.67479391617323 31.18345901893887 20.79918419760547 36-37 21.456708058286786 26.540715281886868 31.08169354534124 20.920883114485108 38-39 21.243595069934912 26.880003469118552 31.17352726825099 20.702874192695546 40-41 21.26669963287211 26.869579023421625 31.107111957570115 20.756609386136148 42-43 21.184289869590582 26.922038365915867 30.913789316249478 20.97988244824407 44-45 21.31752366484398 27.130675261128108 30.58251816041596 20.969282913611952 46-47 21.43761391787981 27.3848441484503 30.03830018786396 21.139241745805933 48-49 21.658350574013223 27.39883252970083 29.799518519917356 21.143298376368588 50-51 21.62309985326188 27.75427729727688 29.48477994178036 21.13784290768088 52-53 21.82397300801954 27.900385939438703 29.246837576708785 21.028803475832973 54-55 21.631912533449714 27.893951284063455 29.26886927717838 21.205266905308452 56-57 21.588408667760557 28.10468624760274 29.205082258675947 21.101822825960756 58-59 21.65457371107558 27.726160650963312 29.27236637249101 21.3468992654701 60-61 21.569734178791098 27.81449727856043 29.588363904940557 21.02740463770792 62-63 21.44726590094268 27.859539866187145 29.678449080193992 21.014745152676188 64-65 21.467898763287213 27.750150724807977 29.5356976495323 21.246252862372515 66-67 21.416771229816515 27.88772645440697 29.34069961489986 21.35480270087665 68-69 21.439782116973642 27.932629158221182 29.285655334679017 21.34193339012616 70-71 21.459295908818135 27.93444764778375 29.151926409923917 21.454330033474196 72-73 21.51846676150789 27.890594072563328 29.068835425295745 21.522103740633032 74-75 21.55882324141568 28.10020996560257 28.811239384567177 21.52972740841457 76-77 21.55693480994686 27.95277242722195 28.946017437916066 21.544275324915123 78-79 21.546023872571443 28.009705138911624 28.833131201224266 21.611139787292675 80-81 21.430494832338642 28.288093711029216 28.82217048071805 21.45924097591409 82-83 21.5517886557017 28.21085468680602 28.74886777342179 21.488488884070488 84-85 21.425259154241974 28.0937116846547 28.758794388098035 21.722234773005294 86-87 21.4741235929437 28.061532091444846 28.75122922900239 21.713115086609065 88-89 21.43027001772328 27.941092128877752 28.698283205969123 21.930354647429844 90-91 21.416035059861287 27.994585727726463 28.78832923532148 21.801049977090774 92-93 21.479366051925325 27.831576679697307 28.876546033557926 21.812511234819443 94-95 21.251288679622704 27.90898879390778 28.915522766789902 21.92419975967961 96-97 21.311578602812503 27.99501733859856 28.64841462681097 22.044989431777964 98-99 21.319825002535435 28.059535508328466 28.400647672470775 22.219991816665328 100-101 21.306066940337974 27.931822824411036 28.79488083545303 21.967229399797965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 531.0 1 380.5 2 296.5 3 383.0 4 316.0 5 247.0 6 443.5 7 1068.0 8 1679.5 9 1961.5 10 1787.0 11 1439.0 12 1427.0 13 1517.5 14 1623.5 15 1701.5 16 1772.5 17 1815.5 18 1890.0 19 1994.5 20 2102.5 21 2316.0 22 2655.5 23 3128.5 24 3675.5 25 4361.0 26 5446.5 27 6656.5 28 7658.0 29 8796.0 30 10123.0 31 11296.5 32 12348.0 33 13369.0 34 14552.5 35 16025.0 36 17797.0 37 19367.5 38 20448.5 39 21091.5 40 21844.5 41 23011.5 42 24244.5 43 25535.0 44 26716.5 45 27270.5 46 27010.5 47 27095.0 48 27817.0 49 27565.0 50 26415.0 51 24832.0 52 23196.0 53 22006.5 54 20828.5 55 19076.5 56 16561.0 57 14238.5 58 12695.5 59 11223.5 60 9276.0 61 7353.5 62 5671.0 63 4243.0 64 3080.5 65 2274.0 66 1699.5 67 1314.5 68 966.0 69 663.5 70 496.0 71 372.0 72 291.5 73 206.5 74 169.0 75 140.5 76 86.0 77 58.5 78 41.5 79 27.0 80 14.5 81 9.5 82 7.0 83 5.0 84 2.5 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020982571875799962 2 0.0 3 0.0 4 0.0 5 0.02140222331331596 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004406340093917991 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001049128593789998 24-25 0.0 26-27 4.1965143751599923E-4 28-29 9.791866875373315E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.69360968779332E-4 42-43 0.0181848956256933 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.994190625266654E-5 82-83 0.0037069210313913263 84-85 7.69360968779332E-4 86-87 0.0 88-89 0.0 90-91 0.014198206969291307 92-93 0.0035670372188859934 94-95 0.0 96-97 0.0 98-99 0.0016086638438113305 100-101 0.02140222331331596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 714879.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.28486579481182 #Duplication Level Percentage of deduplicated Percentage of total 1 84.52357717865912 56.87157546963499 2 9.236966456327249 12.43016096730315 3 2.53823175556948 5.123537490888662 4 1.110350415261545 2.988391147043466 5 0.6528941297559859 2.1964946949425985 6 0.40819565945777353 1.64792340987846 7 0.274135511162825 1.291161975472797 8 0.21708831134542822 1.168540631559957 9 0.15191146738804168 0.9199208426307587 >10 0.8345699083639354 9.887892311984022 >50 0.03749723556415965 1.705819383663127 >100 0.01354040177758712 1.6708915890916343 >500 2.0831387338153242E-4 0.1211014455581419 >1k 8.332554935261297E-4 1.9765886403482837 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4969 0.695082664339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.6633290389002894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3143 0.4396548227042618 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1248 0.17457499800665569 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.12085961400460778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3988381250533308E-4 0.0 5 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 6 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 7 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 8 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 9 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 10-11 2.7976762501066615E-4 0.0 0.0 1.3988381250533308E-4 0.0 12-13 2.7976762501066615E-4 0.0 0.0 2.7976762501066615E-4 0.0 14-15 2.7976762501066615E-4 0.0 0.0 4.1965143751599923E-4 0.0 16-17 2.7976762501066615E-4 0.0 0.0 5.595352500213323E-4 0.0 18-19 2.7976762501066615E-4 0.0 0.0 5.595352500213323E-4 0.0 20-21 2.7976762501066615E-4 0.0 0.0 6.294771562739988E-4 0.0 22-23 2.7976762501066615E-4 0.0 0.0 9.791866875373315E-4 0.0 24-25 2.7976762501066615E-4 0.0 0.0 0.001189012406295331 0.0 26-27 2.7976762501066615E-4 0.0 0.0 0.0015387219375586638 0.0 28-29 4.895933437686657E-4 0.0 0.0 0.0029375600626119942 0.0 30-31 5.595352500213323E-4 0.0 0.0 0.005385526781455323 0.0 32-33 5.595352500213323E-4 0.0 0.0 0.008602854469077983 0.0 34-35 5.595352500213323E-4 0.0 0.0 0.012589543125479975 0.0 36-37 5.595352500213323E-4 0.0 0.0 0.016296464156871304 0.0 38-39 5.595352500213323E-4 0.0 0.0 0.02140222331331596 0.0 40-41 5.595352500213323E-4 0.0 0.0 0.02650798246976062 0.0 42-43 6.294771562739988E-4 0.0 0.0 0.0376287455639346 0.0 44-45 6.994190625266653E-4 0.0 0.0 0.05133735918945724 0.0 46-47 8.393028750319985E-4 0.0 0.0 0.06588527569001187 0.0 48-49 8.393028750319985E-4 0.0 0.0 0.08553895134701117 0.0 50-51 8.393028750319985E-4 0.0 0.0 0.10987873472293913 0.0 52-53 8.393028750319985E-4 0.0 0.0 0.14876643459942174 0.0 54-55 8.393028750319985E-4 0.0 0.0 0.18660500588211432 0.0 56-57 8.393028750319985E-4 0.0 0.0 0.24402731091555352 0.0 58-59 8.393028750319985E-4 0.0 0.0 0.31082183138685004 0.0 60-61 8.393028750319985E-4 0.0 0.0 0.38887699876482595 0.0 62-63 8.393028750319985E-4 0.0 0.0 0.47728356826819646 0.0 64-65 8.393028750319985E-4 0.0 0.0 0.5523312336773076 0.0 66-67 9.791866875373315E-4 0.0 0.0 0.6693440428380187 0.0 68-69 9.791866875373315E-4 0.0 0.0 0.8133544278122591 0.0 70-71 9.791866875373315E-4 0.0 0.0 0.9429567800984502 0.0 72-73 0.001049128593789998 0.0 0.0 1.1208190476989812 0.0 74-75 0.0011190705000426646 0.0 0.0 1.3013391077371135 0.0 76-77 0.0011190705000426646 0.0 0.0 1.4548615919617167 0.0 78-79 0.0011190705000426646 0.0 0.0 1.6346122910310696 0.0 80-81 0.0011190705000426646 0.0 0.0 1.8845846639780999 0.0 82-83 0.0012589543125479975 0.0 0.0 2.119029933737038 0.0 84-85 0.0012589543125479975 0.0 0.0 2.423556993561148 0.0 86-87 0.0012589543125479975 0.0 0.0 2.757879305448894 0.0 88-89 0.0012589543125479975 0.0 0.0 3.1965549414656187 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2380 0.0 52.69373 1 GTATCAA 3850 0.0 36.27593 1 ATCAACG 4935 0.0 28.009632 3 TCAACGC 4945 0.0 27.952991 4 TATCAAC 4995 0.0 27.863375 2 TGGTATC 1095 0.0 27.76307 2 CAACGCA 5010 0.0 27.590328 5 AACGCAG 5150 0.0 26.930649 6 GTGGTAT 1315 0.0 24.20366 1 GAGTACT 4190 0.0 23.865473 12-13 GACACCG 140 1.7770442E-5 23.7521 7 CGCAGAG 5950 0.0 23.392824 8 ACGCAGA 5930 0.0 23.391611 7 GTACTTT 4415 0.0 22.538475 14-15 GCAGAGT 6235 0.0 22.094976 9 GTATAGG 175 4.042604E-6 21.716204 1 CAGAGTA 6035 0.0 21.331627 10-11 GTACATG 2520 0.0 21.112976 1 AGTACTT 4390 0.0 21.100954 12-13 TCGGATA 80 4.9923616E-5 20.78018 54-55 >>END_MODULE