FastQCFastQC Report
Thu 26 May 2016
SRR937881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651750
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35100.5385500575373993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.5067894131185271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.3139240506329114No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9310.14284618335251245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.12811660912926737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.053.8675651
GTATCAA29600.040.318041
TATCAAC38200.030.7267652
ATCAACG38700.030.3274463
TCAACGC39550.029.6756534
CAACGCA39850.029.5714875
AACGCAG41550.028.4693726
GTCCTAC1056.855592E-527.1693551
CGTAGAG908.920566E-426.4004152
ACGCAGA45350.026.0838457
CGCAGAG45950.025.7432528
TGGTATC7800.024.9788552
GAGTACT30000.023.90865312-13
GCGTAGA1000.001643511923.7731881
GCAGAGT48850.023.7287489
GTACATG24500.023.2880171
GTACCTG4700.022.2557491
TACATGG24350.021.8575942
AGTACTT31800.021.80846812-13
GTACTTT33250.021.64231314-15
Produced by FastQC (version 0.11.5)