##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937881_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651750 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59717836593786 33.0 31.0 34.0 30.0 34.0 2 31.720217874952052 33.0 31.0 34.0 30.0 34.0 3 31.721075565784428 33.0 31.0 34.0 30.0 34.0 4 35.321057153816646 37.0 35.0 37.0 33.0 37.0 5 35.311001150747984 37.0 35.0 37.0 33.0 37.0 6 35.35388415803606 37.0 35.0 37.0 33.0 37.0 7 35.291820483314154 37.0 35.0 37.0 33.0 37.0 8 35.27438128116609 37.0 35.0 37.0 33.0 37.0 9 36.893525124664365 39.0 37.0 39.0 33.0 39.0 10-11 36.76969236670503 39.0 37.0 39.0 33.0 39.0 12-13 36.63883851169927 39.0 37.0 39.0 32.0 39.0 14-15 37.763395473724586 40.0 38.0 41.0 32.5 41.0 16-17 37.694332949750674 40.0 37.5 41.0 32.0 41.0 18-19 37.64093287303414 40.0 37.5 41.0 32.0 41.0 20-21 37.564129650939776 40.0 37.0 41.0 32.0 41.0 22-23 37.370606060606065 40.0 37.0 41.0 32.0 41.0 24-25 37.201243574990414 39.5 37.0 41.0 31.5 41.0 26-27 37.12566398158803 39.5 36.5 41.0 31.0 41.0 28-29 37.1536471039509 40.0 37.0 41.0 31.0 41.0 30-31 36.96651860375911 39.0 36.5 41.0 31.0 41.0 32-33 36.702349060222474 39.0 36.0 41.0 30.0 41.0 34-35 36.71604986574607 39.0 36.0 41.0 30.0 41.0 36-37 36.61277406981205 39.0 36.0 41.0 30.0 41.0 38-39 36.469423091676255 39.0 35.0 41.0 30.0 41.0 40-41 36.2274752589183 39.0 35.0 41.0 30.0 41.0 42-43 35.97711852704258 39.0 35.0 41.0 29.0 41.0 44-45 35.73139777522056 39.0 35.0 41.0 27.5 41.0 46-47 35.3588584579977 39.0 35.0 40.5 26.5 41.0 48-49 34.98261296509398 39.0 35.0 40.0 24.0 41.0 50-51 34.04148830072881 38.0 33.5 39.5 23.5 40.5 52-53 34.13879938626774 38.0 33.5 39.5 23.0 40.5 54-55 34.47612965093978 38.0 34.0 40.0 23.0 41.0 56-57 34.2544418872267 38.0 34.0 40.0 22.5 41.0 58-59 34.01915074798619 38.0 34.0 40.0 21.5 41.0 60-61 33.710016110471805 38.0 33.0 40.0 20.0 41.0 62-63 33.350441887226694 37.0 33.0 40.0 18.5 41.0 64-65 32.980604526275414 37.0 32.5 40.0 17.5 41.0 66-67 32.57536708860759 36.0 32.0 39.0 13.5 41.0 68-69 32.089589566551595 36.0 31.0 39.0 8.0 40.5 70-71 31.649663981588034 35.0 31.0 38.5 7.5 40.0 72-73 31.182079018028386 35.0 31.0 38.0 4.5 40.0 74-75 30.715714614499426 35.0 30.0 37.0 2.0 39.0 76-77 30.219836593785963 34.5 30.0 37.0 2.0 39.0 78-79 29.68928039892597 34.0 29.0 36.0 2.0 39.0 80-81 29.139607978519372 34.0 29.0 36.0 2.0 37.5 82-83 28.602718066743385 34.0 28.0 35.0 2.0 37.0 84-85 28.145172228615266 34.0 27.0 35.0 2.0 36.5 86-87 27.657084004602993 33.5 26.0 35.0 2.0 36.0 88-89 27.163835059455312 33.0 26.0 35.0 2.0 36.0 90-91 26.659437667817414 33.0 25.0 35.0 2.0 35.0 92-93 26.0768807057921 32.0 24.0 35.0 2.0 35.0 94-95 25.434225546605294 32.0 21.0 34.5 2.0 35.0 96-97 24.702915995397007 32.0 15.5 34.0 2.0 35.0 98-99 23.750970464135023 31.0 2.0 34.0 2.0 35.0 100-101 21.417762945914845 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08416815071514705 1101 2 0.21140342313711713 1101 3 0.17891997016511496 1101 4 0.11027004089202563 1101 5 0.10474020042251908 1101 6 0.28089754395809763 1101 7 0.16872324801765615 1101 8 0.09962678190387209 1101 9 0.27115336152281344 1101 10-11 0.16488286003401953 1101 12-13 0.23352660720019713 1101 14-15 0.4365954561391945 1101 16-17 0.4700236817221608 1101 18-19 0.40785843374560926 1101 20-21 0.5289254164039647 1101 22-23 0.5496652750109874 1101 24-25 0.36558062218926324 1101 26-27 0.3801437366778302 1101 28-29 0.24584323718787715 1101 30-31 0.5957173503214435 1101 32-33 0.47610884671282605 1101 34-35 0.5823795018600535 1101 36-37 0.4829227786421697 1101 38-39 0.5582736447681427 1101 40-41 0.6542106075313043 1101 42-43 0.6480950207457781 1101 44-45 0.8015367614635309 1101 46-47 0.9076478780009936 1101 48-49 0.8505543612732822 1101 50-51 0.570480921605288 1101 52-53 0.5838526623974047 1101 54-55 0.71820090433242 1101 56-57 0.5979496533398745 1101 58-59 0.6470757942014984 1101 60-61 0.6859426127203889 1101 62-63 0.6000543371041616 1101 64-65 0.4967298741333366 1101 66-67 0.5740685470272524 1101 68-69 0.5807684346579514 1101 70-71 0.6521639427515282 1101 72-73 0.6196311180606315 1101 74-75 0.7049053899224695 1101 76-77 0.7791723992946622 1101 78-79 0.7835598951309102 1101 80-81 0.6711663619650956 1101 82-83 0.6994117981003107 1101 84-85 0.7089931138117969 1101 86-87 0.7492449218926787 1101 88-89 0.9102326170333193 1101 90-91 1.0094371721648479 1101 92-93 1.0098418019227822 1101 94-95 1.17408367205201 1101 96-97 1.4061788614394644 1101 98-99 1.5639469088343212 1101 100-101 1.8217112725861426 1102 1 0.12845770097721854 1102 2 0.18138875765015428 1102 3 0.29488860299134245 1102 4 0.3390114203733461 1102 5 0.15825380208321604 1102 6 0.06939554907724244 1102 7 -0.035020142321194214 1102 8 0.21562296072364973 1102 9 0.2566790437544384 1102 10-11 0.2466129247230242 1102 12-13 0.34968227732259294 1102 14-15 0.45310900380974317 1102 16-17 0.5017594208858398 1102 18-19 0.4236753432393954 1102 20-21 0.6023594542168951 1102 22-23 0.6452124100623777 1102 24-25 0.3901285097972078 1102 26-27 0.516100293340493 1102 28-29 0.32001137572879657 1102 30-31 0.5235647521282445 1102 32-33 0.4138959448305499 1102 34-35 0.6814811719185485 1102 36-37 0.7019682438554327 1102 38-39 0.5429230889450807 1102 40-41 0.5017225983022939 1102 42-43 0.7591409090655077 1102 44-45 0.8543044176190122 1102 46-47 0.834943258097141 1102 48-49 0.6989279907223747 1102 50-51 0.7757431980282306 1102 52-53 0.7320104414410764 1102 54-55 0.9499023762801286 1102 56-57 0.9037930951346382 1102 58-59 0.7985578237462292 1102 60-61 0.8540288162544343 1102 62-63 0.8262543232209865 1102 64-65 0.9494626019690031 1102 66-67 0.9424887732790239 1102 68-69 0.9764087853515058 1102 70-71 0.8315280269629284 1102 72-73 0.7271971210157631 1102 74-75 0.8002748533154964 1102 76-77 0.9171596466014869 1102 78-79 0.7866728678614763 1102 80-81 0.8623096639363972 1102 82-83 0.83441746706578 1102 84-85 0.8428878528575794 1102 86-87 1.0174968972158744 1102 88-89 1.3248108382893555 1102 90-91 1.1471451030971203 1102 92-93 1.3153475298366786 1102 94-95 1.1583896176894406 1102 96-97 1.5261019970990723 1102 98-99 1.6108913419543 1102 100-101 2.1345334918456373 1103 1 0.3961912051869305 1103 2 0.30816039831615427 1103 3 0.2583779006686022 1103 4 0.321152658027799 1103 5 0.28184381176190954 1103 6 0.23602678169163482 1103 7 0.3371459388163416 1103 8 0.347055542111697 1103 9 0.1121378453500057 1103 10-11 0.33688835881494583 1103 12-13 0.4784680836802053 1103 14-15 0.49552988304008494 1103 16-17 0.32501312919570324 1103 18-19 0.3761906158115025 1103 20-21 0.22377993351325642 1103 22-23 0.520368703967776 1103 24-25 0.5228337435801791 1103 26-27 0.2593705491049718 1103 28-29 0.2706232449964716 1103 30-31 0.26993300956084454 1103 32-33 0.34005497285715336 1103 34-35 0.35688716438670554 1103 36-37 0.3333710903744489 1103 38-39 0.4338507573894006 1103 40-41 0.4769738951142699 1103 42-43 0.2713957636545672 1103 44-45 0.07920335623285979 1103 46-47 0.26394308117467347 1103 48-49 0.4349500004724405 1103 50-51 0.49857630661551866 1103 52-53 0.3389924117032308 1103 54-55 0.3882883824560608 1103 56-57 0.24116231746373984 1103 58-59 -0.012842079020749964 1103 60-61 0.017151334322079492 1103 62-63 0.044332082877012624 1103 64-65 0.08617721115618338 1103 66-67 -0.09044779778508882 1103 68-69 0.15932469068162547 1103 70-71 0.33074599990717246 1103 72-73 0.15151667570636462 1103 74-75 0.1343754701653559 1103 76-77 0.15318574489604586 1103 78-79 0.23637522182238513 1103 80-81 0.0678184944491278 1103 82-83 0.10333739255019836 1103 84-85 0.33455725761010413 1103 86-87 0.48520036920785614 1103 88-89 0.3171028168624055 1103 90-91 0.1896696826832418 1103 92-93 0.26605455068527917 1103 94-95 0.3318111793350269 1103 96-97 0.4840204670616508 1103 98-99 1.046015804682387 1103 100-101 1.7983086902887209 1104 1 0.0212947509996404 1104 2 0.18211624121835612 1104 3 0.22314314070407448 1104 4 0.2236096963369434 1104 5 0.0792778960128473 1104 6 0.19009621631466445 1104 7 0.19356914654776602 1104 8 0.19139223156405905 1104 9 0.3583826961205716 1104 10-11 0.12627056352698673 1104 12-13 0.19479577110093516 1104 14-15 0.4531198752951937 1104 16-17 0.37307410056205725 1104 18-19 0.26172282445210726 1104 20-21 0.41047275321445653 1104 22-23 0.4767397178748993 1104 24-25 0.42725282070357196 1104 26-27 0.2968025007050201 1104 28-29 0.2802987453275918 1104 30-31 0.28508830021871034 1104 32-33 0.2542264379883079 1104 34-35 0.22655525656212205 1104 36-37 0.3248146705340602 1104 38-39 0.5855456536190999 1104 40-41 0.5083120774222465 1104 42-43 0.35810766496895496 1104 44-45 0.3697523454685907 1104 46-47 0.19535927360712435 1104 48-49 0.26048007646215865 1104 50-51 0.24211044586639474 1104 52-53 0.24370725383094793 1104 54-55 0.18450404958293376 1104 56-57 0.3583969427345366 1104 58-59 0.15361917969224947 1104 60-61 0.364921591699364 1104 62-63 0.4457632038656598 1104 64-65 0.4374758833003938 1104 66-67 0.36388204473548313 1104 68-69 0.36459963427862974 1104 70-71 0.31445254714539317 1104 72-73 0.5641071730630003 1104 74-75 0.6746303780685103 1104 76-77 0.5032912549924617 1104 78-79 0.6461725077435219 1104 80-81 0.4230867033591217 1104 82-83 0.5589344786756207 1104 84-85 0.772901759299387 1104 86-87 0.8046187167401548 1104 88-89 1.033181882087849 1104 90-91 1.0320387210821842 1104 92-93 1.1715377083555296 1104 94-95 1.2546905947641953 1104 96-97 1.358004371159712 1104 98-99 1.6311983708226236 1104 100-101 1.7855329890394351 1105 1 0.1550160735367534 1105 2 0.058049664520201105 1105 3 0.19024278083002955 1105 4 -0.04858441072558861 1105 5 0.198947261734844 1105 6 0.0899781326439495 1105 7 0.26900774303899055 1105 8 0.29723456174849616 1105 9 0.0905970460980754 1105 10-11 0.19595960986076477 1105 12-13 0.3787307688517174 1105 14-15 0.13991137327090541 1105 16-17 0.18412898135377986 1105 18-19 0.19127748032255454 1105 20-21 0.10242338299403286 1105 22-23 0.14451974486545538 1105 24-25 0.20228093586326423 1105 26-27 0.10648255018769959 1105 28-29 0.304027940109421 1105 30-31 0.3939333294584699 1105 32-33 0.48291233542420997 1105 34-35 0.3144207536381458 1105 36-37 0.40016048699830264 1105 38-39 0.5847809212754171 1105 40-41 0.5688552623075367 1105 42-43 0.5267002200746589 1105 44-45 0.5025571137996749 1105 46-47 0.5796682029818427 1105 48-49 0.7585710571189281 1105 50-51 0.4938086015119225 1105 52-53 0.612294185904716 1105 54-55 0.7125292407660169 1105 56-57 0.7887163309486596 1105 58-59 0.43750919318753034 1105 60-61 0.5341720415419218 1105 62-63 0.5935870905053378 1105 64-65 0.7349511419598684 1105 66-67 0.5277343839167656 1105 68-69 0.8111330350883428 1105 70-71 0.8316534018462463 1105 72-73 0.83581769937879 1105 74-75 0.9214589880550186 1105 76-77 0.9358429868862999 1105 78-79 0.799470415975641 1105 80-81 0.7995458801472424 1105 82-83 1.1476293729626228 1105 84-85 1.1507160867847688 1105 86-87 0.9988129362633238 1105 88-89 1.2363532721013435 1105 90-91 1.4139409927870865 1105 92-93 2.097940654824267 1105 94-95 1.8613032803242504 1105 96-97 1.7898026167737449 1105 98-99 2.0564401611959937 1105 100-101 2.4375825841811363 1106 1 -0.16802282259536483 1106 2 0.03735214170403367 1106 3 -0.18494735389400319 1106 4 -0.037040516766438714 1106 5 -0.11124395947332744 1106 6 -0.11952317113701838 1106 7 -0.10387361289321007 1106 8 -0.16976348258005913 1106 9 -0.24949965099014548 1106 10-11 -0.43701246749689915 1106 12-13 -0.38623555014002164 1106 14-15 -0.43206820082730957 1106 16-17 -0.4579559817057586 1106 18-19 -0.33197328021633865 1106 20-21 -0.2806301806739384 1106 22-23 -0.393045444182782 1106 24-25 -0.3444378820419942 1106 26-27 -0.25758470318040594 1106 28-29 -0.054267366279106 1106 30-31 -0.13113729201045743 1106 32-33 0.15980553403350228 1106 34-35 -0.019265687039819568 1106 36-37 -0.22409255515728432 1106 38-39 0.03279265530409958 1106 40-41 0.1446375308864134 1106 42-43 0.08970271898817828 1106 44-45 -0.05839464630462743 1106 46-47 -0.12286602126847868 1106 48-49 -0.300683071520794 1106 50-51 -0.34389783047416955 1106 52-53 -0.17235107702444452 1106 54-55 -0.17317262798670185 1106 56-57 -0.1804275195511238 1106 58-59 -0.03166181941568169 1106 60-61 -0.11115879287789454 1106 62-63 -0.1141758386558962 1106 64-65 -0.042862851955817405 1106 66-67 -0.14828962738266682 1106 68-69 -0.3936556437469463 1106 70-71 0.1696314548666713 1106 72-73 -0.05542523847431724 1106 74-75 -0.15576287200582684 1106 76-77 -0.057393649358811416 1106 78-79 0.3569590078922005 1106 80-81 0.5752276489434216 1106 82-83 -0.1749613484628476 1106 84-85 -0.07968645558811005 1106 86-87 0.19762595234329083 1106 88-89 0.44493106436557994 1106 90-91 0.724661875012206 1106 92-93 0.6453792684705064 1106 94-95 0.8274864615498032 1106 96-97 0.896794142197475 1106 98-99 1.2235142594141593 1106 100-101 2.0785674820516284 1107 1 -0.16527188831579664 1107 2 -0.05519343981421798 1107 3 0.1370440771580519 1107 4 0.08962514326904625 1107 5 -0.16503109472680677 1107 6 -0.011326047585619392 1107 7 -0.08177176698328026 1107 8 -0.07008812113654983 1107 9 -0.22970519249480503 1107 10-11 0.01144880236833501 1107 12-13 0.004269715799360085 1107 14-15 0.16344301052748023 1107 16-17 0.1630922044706793 1107 18-19 0.2917857322784698 1107 20-21 0.31767915079713305 1107 22-23 0.3911147422956489 1107 24-25 0.4170495099024478 1107 26-27 0.392161908876524 1107 28-29 0.06610606243253159 1107 30-31 -0.16086436913098368 1107 32-33 0.4365575441501548 1107 34-35 0.46503322579850703 1107 36-37 0.5184379268932204 1107 38-39 0.6556221068159331 1107 40-41 0.1257230882455218 1107 42-43 0.379076940421875 1107 44-45 0.2314632299552315 1107 46-47 0.47162361672177866 1107 48-49 0.7264251916629263 1107 50-51 0.6032469545471528 1107 52-53 0.259189351800444 1107 54-55 0.6150762112417212 1107 56-57 0.9698310588339893 1107 58-59 1.1244666770548122 1107 60-61 1.2894348049477813 1107 62-63 1.2216316053817025 1107 64-65 1.2428497485500145 1107 66-67 1.112761295572298 1107 68-69 1.6336106919897198 1107 70-71 1.6725569767420296 1107 72-73 1.684323638502768 1107 74-75 1.3875088995392275 1107 76-77 1.639682716199303 1107 78-79 1.8377874024412932 1107 80-81 1.7468236626839335 1107 82-83 1.5605573182541512 1107 84-85 2.1691598507045917 1107 86-87 1.9666135330310688 1107 88-89 2.01362184614052 1107 90-91 2.1088631131685602 1107 92-93 1.9749982900249954 1107 94-95 1.9363418275231545 1107 96-97 2.1187280065287553 1107 98-99 2.4831826373538384 1107 100-101 2.9217264794469706 1108 1 0.27255333223533995 1108 2 0.21928888296765692 1108 3 0.20656546462982917 1108 4 0.2591042297719781 1108 5 0.2591405962416786 1108 6 0.43418469838941576 1108 7 0.3571084295378455 1108 8 0.32833604036141395 1108 9 0.43959296817752147 1108 10-11 0.5073282802826427 1108 12-13 0.5018641667276853 1108 14-15 0.4333639637772748 1108 16-17 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0.2730291907601696 2316 8 -0.02351456178212885 2316 9 0.2914589076502878 2316 10-11 0.2838235165081855 2316 12-13 -0.02653749951976181 2316 14-15 0.0710870452307617 2316 16-17 0.11783524381339561 2316 18-19 -0.0053953731867650845 2316 20-21 -0.38350369819109176 2316 22-23 0.006733603392987675 2316 24-25 0.02307302357894514 2316 26-27 0.09133552674644108 2316 28-29 0.3452220007404847 2316 30-31 0.259829218645244 2316 32-33 0.08729167480689881 2316 34-35 -0.1932870211607849 2316 36-37 0.058425507277391375 2316 38-39 -0.3641776268788419 2316 40-41 -0.36769093278423526 2316 42-43 0.1194233760518415 2316 44-45 0.160450493878038 2316 46-47 -0.03443393147887264 2316 48-49 0.2125456738783953 2316 50-51 0.0439134341234535 2316 52-53 0.38382139082669653 2316 54-55 0.07638433116266441 2316 56-57 0.08255698537912792 2316 58-59 0.12576536284450412 2316 60-61 0.28903773549944134 2316 62-63 0.32136171757620957 2316 64-65 0.41653927009625136 2316 66-67 0.538151050583437 2316 68-69 0.6769838118808131 2316 70-71 0.3338746502136054 2316 72-73 0.574144330299255 2316 74-75 0.3257004124036804 2316 76-77 0.1921973898559841 2316 78-79 0.3008689001991911 2316 80-81 0.722169531071831 2316 82-83 0.4841527970363124 2316 84-85 0.22553177043088368 2316 86-87 0.16962349857857717 2316 88-89 -0.24228493508914184 2316 90-91 -0.23056259901878562 2316 92-93 -0.4731199590480095 2316 94-95 -0.5570303967225634 2316 96-97 -0.19964108295815564 2316 98-99 0.48549340241411443 2316 100-101 0.732228817081392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3330.0 3 722.0 4 631.0 5 681.0 6 806.0 7 950.0 8 1140.0 9 1354.0 10 1810.0 11 2216.0 12 2823.0 13 3700.0 14 4042.0 15 4785.0 16 5954.0 17 7565.0 18 6915.0 19 4948.0 20 4972.0 21 4976.0 22 5585.0 23 6020.0 24 6606.0 25 7580.0 26 8588.0 27 10095.0 28 11863.0 29 13909.0 30 16612.0 31 20909.0 32 26028.0 33 33346.0 34 44564.0 35 62388.0 36 87804.0 37 119372.0 38 94327.0 39 11832.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49550636681407 18.995008442396994 15.192466642142413 22.317018548646523 2 15.030039457566188 20.140594510412242 41.45583645731381 23.37352957470776 3 18.895218223559898 24.53176259147786 30.710711450043394 25.86230773491885 4 12.330857838272784 17.05722255300621 37.97646356529753 32.635456043423474 5 13.206676338506176 37.06140182185456 34.96169982322995 14.770222016409306 6 30.767882214898755 36.46875370039168 18.048684467504987 14.714679617204574 7 27.39245356709572 33.23518060358547 21.30677930397157 18.06558652534724 8 25.498666633857965 35.46184911893257 20.16966199860358 18.869822248605885 9 25.60467530470222 17.042254604175476 21.121919335614596 36.231150755507706 10-11 24.305254383779477 27.077700780329668 27.7047788655846 20.912265970306258 12-13 25.218178267214746 24.59828286975738 28.409924477640864 21.773614385387017 14-15 22.249184220157527 25.89489741626147 27.020304537304433 24.835613826276568 16-17 21.138858988159313 29.157850222974012 27.766338612947873 21.936952175918805 18-19 21.091221678959908 27.298687738307326 30.646811919921817 20.963278662810946 20-21 21.7606327324333 26.449928542379865 30.93965040733534 20.849788317851488 22-23 21.490546674712597 26.342395252389757 30.904513535224638 21.26254453767301 24-25 21.24179389019572 26.4830189259413 31.044847254892915 21.230339928970064 26-27 21.04483797084713 26.70611327624954 30.86676697463828 21.382281778265053 28-29 20.93492324574051 26.838730315452587 30.988989187580597 21.237357251226307 30-31 21.12870559324093 26.712565761448758 30.884544348705994 21.274184296604318 32-33 20.8852987200732 26.772193023264755 30.812517061205032 21.52999119545701 34-35 21.380597846939065 26.833911670832798 30.657936115555756 21.12755436667238 36-37 21.358510524620485 26.689739523234422 30.538042922585753 21.413707029559344 38-39 21.136522628208578 26.885402172492523 30.650056503255662 21.328018696043234 40-41 21.270006234112046 26.883781853737432 30.591656436624604 21.254555475525923 42-43 21.21145025608854 26.871845021457315 30.298480382433755 21.61822434002039 44-45 21.202679078006007 27.12251277343924 30.210586847854998 21.46422130069975 46-47 21.368111431782783 27.397285549324923 29.644080071220884 21.590522947671406 48-49 21.375671058115056 27.479128189592466 29.506950093729 21.63825065856348 50-51 21.481234274746704 27.651223878393427 29.307027688749177 21.560514158110696 52-53 21.53559823883409 27.732825748399375 29.089308030993443 21.642267981773085 54-55 21.484433263778797 27.664612834426883 29.09542708382988 21.755526817964448 56-57 21.39092673366255 27.90231155006437 29.09086716753454 21.61589454873854 58-59 21.54274677207742 27.60176439527804 29.11590392208253 21.73958491056201 60-61 21.348663527451226 27.509719730476373 29.48164512107195 21.65997162100046 62-63 21.289943675852108 27.73235921077008 29.489116071407977 21.48858104196983 64-65 21.265632085841 27.567582109284327 29.392241412441106 21.77454439243357 66-67 21.109209927560293 27.866303238263907 29.173277250980828 21.851209583194972 68-69 21.136226491965086 27.741114702683173 29.292366350599785 21.830292454751955 70-71 21.293771118825003 27.71381705371061 29.165347140445757 21.82706468701863 72-73 21.246508353049514 27.671984270101664 29.12337178180454 21.958135595044283 74-75 21.20973128954422 27.967195447518943 28.875170360599483 21.94790290233735 76-77 21.29176504779574 27.770042263841084 28.93946744638597 21.998725241977212 78-79 21.34507240426445 27.751508133850628 28.84637563489244 22.05704382699248 80-81 21.233272163542445 27.99944032332091 28.82204203571629 21.945245477420354 82-83 21.241441660298683 27.943277023442892 28.843661255033524 21.971620061224897 84-85 21.268578412720377 27.824014820451914 28.826240194326257 22.081166572501452 86-87 21.389438728250383 27.77247796692252 28.813615346475668 22.02446795835143 88-89 21.329377739714968 27.70596583265041 28.74849897061288 22.216157457021744 90-91 21.236195623344724 27.619208696420955 28.93028333326927 22.214312346965055 92-93 21.397096921494494 27.473082487588655 28.921623645283844 22.208196945633006 94-95 21.202800944307825 27.630766723527056 28.775174943709754 22.39125738845537 96-97 21.112234191785458 27.68394716311103 28.770662229003406 22.433156416100108 98-99 21.259877021046634 27.722075867513585 28.613169771376608 22.404877340063173 100-101 21.45419467407638 27.397261326907486 28.86283190942317 22.285712089592966 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1138.0 1 687.5 2 280.5 3 340.5 4 291.0 5 267.0 6 422.0 7 816.5 8 1321.5 9 1593.5 10 1411.0 11 1141.0 12 1112.0 13 1165.5 14 1292.5 15 1347.5 16 1356.0 17 1451.5 18 1547.5 19 1609.5 20 1699.0 21 1969.0 22 2318.5 23 2767.0 24 3312.0 25 3969.0 26 4926.5 27 5986.5 28 6852.5 29 7765.5 30 8994.0 31 10051.0 32 11067.5 33 12122.0 34 13341.0 35 14697.0 36 16074.5 37 17568.5 38 18617.5 39 19301.0 40 19991.0 41 21090.0 42 22239.5 43 23143.5 44 24040.5 45 24452.0 46 24285.5 47 24577.5 48 25261.0 49 25134.0 50 24130.0 51 22723.0 52 21471.5 53 20537.0 54 19402.0 55 17489.5 56 15296.0 57 13481.5 58 12047.0 59 10507.0 60 8784.5 61 7079.5 62 5399.0 63 4048.5 64 3038.5 65 2255.5 66 1632.0 67 1250.5 68 948.0 69 666.0 70 491.0 71 370.0 72 291.5 73 224.0 74 161.0 75 113.0 76 75.0 77 53.5 78 35.0 79 24.5 80 21.5 81 16.0 82 9.0 83 7.0 84 7.5 85 7.0 86 4.0 87 2.5 88 2.5 89 2.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31499808208668967 2 0.29735327963176067 3 0.2863060989643268 4 0.2729574223245109 5 0.2689681626390487 6 0.2272343690065209 7 0.21526658995013426 8 0.23260452627541237 9 0.23398542385884158 10-11 0.23199079401611047 12-13 0.22692750287686994 14-15 0.22278481012658227 16-17 0.22247794399693133 18-19 0.22408899117759878 20-21 0.20935941695435367 22-23 0.2021480629075566 24-25 0.20253164556962028 26-27 0.21779823551975452 28-29 0.23406214039125434 30-31 0.22754123513617186 32-33 0.23283467587265055 34-35 0.2250095895665516 36-37 0.20659762178749522 38-39 0.206367472190257 40-41 0.19892596854622172 42-43 0.21311852704257767 44-45 0.21127733026467202 46-47 0.21158419639432297 48-49 0.22570003835826621 50-51 0.23352512466436517 52-53 0.24741081703107018 54-55 0.21802838511699268 56-57 0.18603759110088225 58-59 0.18649789029535865 60-61 0.1947065592635213 62-63 0.20230149597238203 64-65 0.19102416570771003 66-67 0.1960874568469505 68-69 0.2006137322593019 70-71 0.19393939393939394 72-73 0.1947065592635213 74-75 0.19815880322209437 76-77 0.21948599923283466 78-79 0.22140391254315303 80-81 0.21089374760260834 82-83 0.20882240122746454 84-85 0.19907940161104717 86-87 0.2006137322593019 88-89 0.20912926735711543 90-91 0.20981971614883005 92-93 0.20514000767165325 94-95 0.2035289604909858 96-97 0.1929420790180284 98-99 0.2015343306482547 100-101 0.19217491369390105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 651750.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.3396670141263 #Duplication Level Percentage of deduplicated Percentage of total 1 85.91188938638423 60.43013691992719 2 8.410211312463531 11.831429264742459 3 2.32464221329845 4.905436776311837 4 1.039930795025719 2.925935433593789 5 0.5747318531230932 2.0213223585545057 6 0.37834431965462634 1.5967568076715546 7 0.26676881136721653 1.3135100552926995 8 0.18957271981911325 1.0667585589630952 9 0.14235768014369826 0.9012052636390112 >10 0.7242423388227406 8.938827054706637 >50 0.02705422580222114 1.3121368745116344 >100 0.008945278891923049 1.0435643859057149 >500 6.545326017187902E-4 0.3534307030059337 >1k 6.545326017187902E-4 1.3595495431739328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3510 0.5385500575373993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.5067894131185271 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2046 0.3139240506329114 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 931 0.14284618335251245 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.12811660912926737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.5343306482546988E-4 0.0 0.0 0.0 0.0 14-15 3.0686612965093976E-4 0.0 0.0 0.0 0.0 16-17 3.0686612965093976E-4 0.0 0.0 0.0 0.0 18-19 4.6029919447640965E-4 0.0 0.0 0.0 0.0 20-21 6.137322593018795E-4 0.0 0.0 0.0 0.0 22-23 6.137322593018795E-4 0.0 0.0 1.5343306482546988E-4 0.0 24-25 6.137322593018795E-4 0.0 0.0 3.0686612965093976E-4 0.0 26-27 6.137322593018795E-4 0.0 0.0 9.205983889528193E-4 0.0 28-29 6.137322593018795E-4 0.0 0.0 0.0026083621020329883 0.0 30-31 6.137322593018795E-4 0.0 0.0 0.005370157268891446 0.0 32-33 6.137322593018795E-4 0.0 0.0 0.008515535097813578 0.0 34-35 6.137322593018795E-4 0.0 0.0 0.011047180667433832 0.0 36-37 6.137322593018795E-4 0.0 0.0 0.01442270809359417 0.0 38-39 7.671653241273494E-4 0.0 0.0 0.02079018028385117 0.0 40-41 7.671653241273494E-4 0.0 0.0 0.026850786344457228 0.0 42-43 7.671653241273494E-4 0.0 0.0 0.040352896049098586 0.0 44-45 7.671653241273494E-4 0.0 0.0 0.054698887610280016 0.0 46-47 9.205983889528193E-4 0.0 0.0 0.07196010740314537 0.0 48-49 9.205983889528193E-4 0.0 0.0 0.08968162639048716 0.0 50-51 9.205983889528193E-4 0.0 0.0 0.11223628691983123 0.0 52-53 9.973149213655542E-4 0.0 0.0 0.15726889144610665 0.0 54-55 0.0010740314537782893 0.0 0.0 0.193632527809743 0.0 56-57 0.0010740314537782893 0.0 0.0 0.2569236670502493 0.0 58-59 0.0010740314537782893 0.0 0.0 0.33762945914844644 0.0 60-61 0.0010740314537782893 0.0 0.0 0.41419255849635594 0.0 62-63 0.0010740314537782893 0.0 0.0 0.5023398542385884 0.0 64-65 0.0010740314537782893 0.0 0.0 0.5777522056003068 0.0 66-67 0.0010740314537782893 0.0 0.0 0.701035673187572 0.0 68-69 0.0010740314537782893 0.0 0.0 0.8537782892213273 0.0 70-71 0.0010740314537782893 0.0 0.0 0.9874184886843115 0.0 72-73 0.0010740314537782893 0.0 0.0 1.1723820483314156 0.0 74-75 0.0010740314537782893 0.0 0.0 1.3568085922516304 0.0 76-77 0.0010740314537782893 0.0 0.0 1.513233601841197 0.0 78-79 0.0010740314537782893 0.0 0.0 1.70126582278481 0.0 80-81 0.001380897583429229 0.0 0.0 1.9617951668584581 0.0 82-83 0.001380897583429229 0.0 0.0 2.209205983889528 0.0 84-85 0.001380897583429229 7.671653241273494E-5 0.0 2.5311085538933638 0.0 86-87 0.001380897583429229 1.5343306482546988E-4 0.0 2.878481012658228 0.0 88-89 0.001380897583429229 1.5343306482546988E-4 0.0 3.3387802071346373 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1730 0.0 53.867565 1 GTATCAA 2960 0.0 40.31804 1 TATCAAC 3820 0.0 30.726765 2 ATCAACG 3870 0.0 30.327446 3 TCAACGC 3955 0.0 29.675653 4 CAACGCA 3985 0.0 29.571487 5 AACGCAG 4155 0.0 28.469372 6 GTCCTAC 105 6.855592E-5 27.169355 1 CGTAGAG 90 8.920566E-4 26.400415 2 ACGCAGA 4535 0.0 26.083845 7 CGCAGAG 4595 0.0 25.743252 8 TGGTATC 780 0.0 24.978855 2 GAGTACT 3000 0.0 23.908653 12-13 GCGTAGA 100 0.0016435119 23.773188 1 GCAGAGT 4885 0.0 23.728748 9 GTACATG 2450 0.0 23.288017 1 GTACCTG 470 0.0 22.255749 1 TACATGG 2435 0.0 21.857594 2 AGTACTT 3180 0.0 21.808468 12-13 GTACTTT 3325 0.0 21.642313 14-15 >>END_MODULE