FastQCFastQC Report
Thu 26 May 2016
SRR937881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651750
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44910.6890678941311853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42660.6545454545454545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.4222477943996932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.16678174146528577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.11415420023014959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.048.066541
GTATCAA34200.035.4261931
TGGTATC10600.028.2322252
ATCAACG42750.028.1122023
TATCAAC43850.027.515322
TCAACGC44100.027.2516234
AGTACCG1056.8995025E-527.143915
CAACGCA44350.027.0980075
AACGCAG45600.026.4573296
TGAACCG1109.4679606E-525.9100975
GTGGTAT11800.025.3669911
GTACATG23550.024.2103311
ACGCAGA51800.023.2906237
TACATGG23600.023.1470852
CGCAGAG52150.022.9521488
GTACTAG1252.250345E-422.8061331
ACATGGG23550.022.5911083
GAGTACT36500.022.18751312-13
GCAGAGT55250.021.4923979
GGTCTAA1604.9747156E-520.786841
Produced by FastQC (version 0.11.5)