FastQCFastQC Report
Thu 26 May 2016
SRR937880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419545
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.573240057681536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.5150818148232014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.30676089573228144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6180.14730243478053606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.1308560464312529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.050.4886671
GTATCAA20000.040.900331
ATCAACG24800.032.7688643
TATCAAC25300.032.1212542
CAACGCA25200.032.0639655
TCAACGC25500.032.0633544
AACGCAG25650.031.6678226
ACGCAGA28600.027.9064467
CGCAGAG28950.027.5690638
TACTAGA1457.832259E-726.2203732
GTGTAAG1309.906127E-625.6084351
GCAGAGT30550.025.5031559
TGGTATC5250.025.3463632
GTACATG15250.023.3894351
GAGTACT19450.023.32347112-13
ACATGGG15000.022.8117283
TACATGG15100.022.6606542
AGTACTT20150.022.63109612-13
TAGACTG1903.256646E-722.5142615
AGAGTAC28000.021.9695510-11
Produced by FastQC (version 0.11.5)