FastQCFastQC Report
Thu 26 May 2016
SRR937880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419545
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.7029043368411017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.6917017244872421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.45144144251510565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.17876509075307773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.12465885661847956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.046.080781
GTATCAA22350.039.33491
CAGTCCG651.3270507E-436.537049
ATCAACG28850.030.4580883
TATCAAC29200.030.2556742
TCAACGC29300.029.9903014
AACGCAG30150.029.3023436
CAACGCA30000.029.2905275
TGTCGAG700.00838719427.1418022
TGGTATC6650.027.1418022
GTGGTAT6850.027.0556411
ACGCAGA34000.025.8445847
CGCAGAG33950.025.7427398
GCAGAGT36100.023.4201419
AGAGTAC31900.023.0790410-11
GAGTACT25550.022.49423212-13
TACCTGG3200.022.264762
AGTACTT26800.022.06537212-13
CGCAGCG550.002873396521.59521726-27
CGTCTAT1553.8999882E-521.4507796
Produced by FastQC (version 0.11.5)