##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937880_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419545 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07618014754079 33.0 31.0 34.0 30.0 34.0 2 32.318893086558056 34.0 31.0 34.0 31.0 34.0 3 32.39270876783182 34.0 31.0 34.0 30.0 34.0 4 36.05152248268958 37.0 35.0 37.0 35.0 37.0 5 35.87464038422577 37.0 35.0 37.0 35.0 37.0 6 35.96586301826979 37.0 35.0 37.0 35.0 37.0 7 35.92213946060613 37.0 35.0 37.0 35.0 37.0 8 35.92165321955928 37.0 35.0 37.0 35.0 37.0 9 37.57260842102754 39.0 37.0 39.0 35.0 39.0 10-11 37.51601735213148 39.0 37.0 39.0 35.0 39.0 12-13 37.431344671012646 39.0 37.0 39.0 34.5 39.0 14-15 38.60516273582095 40.0 38.0 41.0 34.0 41.0 16-17 38.55299550703738 40.0 38.0 41.0 34.0 41.0 18-19 38.55569605167503 40.0 38.0 41.0 34.0 41.0 20-21 38.48503855367124 40.0 38.0 41.0 34.0 41.0 22-23 38.33703416796768 40.0 38.0 41.0 34.0 41.0 24-25 38.26287525771967 40.0 38.0 41.0 33.5 41.0 26-27 38.15381663468758 40.0 38.0 41.0 33.5 41.0 28-29 38.031165905921895 40.0 37.0 41.0 33.0 41.0 30-31 38.07300289599446 40.0 37.0 41.0 34.0 41.0 32-33 37.96057514688532 40.0 37.0 41.0 33.0 41.0 34-35 37.801415819518766 40.0 37.0 41.0 33.0 41.0 36-37 37.61761193674099 40.0 37.0 41.0 33.0 41.0 38-39 37.470874399647236 40.0 36.5 41.0 33.0 41.0 40-41 37.27687256432564 40.0 36.0 41.0 32.0 41.0 42-43 37.04866820007389 40.0 36.0 41.0 31.5 41.0 44-45 36.70750336674254 39.0 36.0 41.0 31.0 41.0 46-47 36.2922547045013 39.0 35.0 41.0 30.0 41.0 48-49 35.902184509409004 39.0 35.0 41.0 28.5 41.0 50-51 35.60762850230607 39.0 35.0 40.0 27.5 41.0 52-53 35.37602402602819 39.0 35.0 40.0 27.0 41.0 54-55 35.146058229748895 38.5 35.0 40.0 26.5 41.0 56-57 34.861194865866594 38.0 34.0 40.0 26.0 41.0 58-59 34.59610768809067 38.0 34.0 40.0 26.0 41.0 60-61 34.25092183198465 38.0 34.0 40.0 25.0 41.0 62-63 33.875196939541645 37.0 33.5 40.0 23.5 41.0 64-65 33.47560929101765 37.0 33.0 39.5 22.5 41.0 66-67 33.02937467971255 36.0 32.5 39.0 21.5 40.5 68-69 32.56006864579485 36.0 32.0 39.0 20.0 40.0 70-71 32.14352095722747 35.0 31.5 38.0 18.5 40.0 72-73 31.67857679152415 35.0 31.0 37.5 17.5 40.0 74-75 31.16351642851184 35.0 30.5 37.0 13.0 39.0 76-77 29.944722258637334 33.5 29.5 35.5 11.0 38.5 78-79 30.47925490710174 34.0 30.0 36.0 8.0 38.5 80-81 30.26732412494488 34.0 30.0 36.0 7.0 37.5 82-83 29.90654756939065 34.0 30.0 35.0 4.5 37.0 84-85 29.508098058611115 34.0 29.5 35.0 2.0 36.5 86-87 29.055153797566412 34.0 29.0 35.0 2.0 36.0 88-89 28.590171495310397 34.0 29.0 35.0 2.0 36.0 90-91 28.055191934119105 33.0 27.5 35.0 2.0 35.0 92-93 27.574558152284023 33.0 26.5 35.0 2.0 35.0 94-95 27.05376777222944 33.0 26.0 35.0 2.0 35.0 96-97 26.462623794825348 33.0 25.0 34.5 2.0 35.0 98-99 25.62220858310789 32.0 23.5 34.0 2.0 35.0 100-101 23.271475050352166 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08789377080908167 1101 2 -0.0036833301340521984 1101 3 0.03634193587170387 1101 4 0.05550509809287263 1101 5 -0.08291584330666524 1101 6 -0.017587711545751006 1101 7 7.500960360857789E-4 1101 8 0.0057362213471634504 1101 9 0.17343646632879484 1101 10-11 0.056978490039973906 1101 12-13 0.10714247921688269 1101 14-15 0.05445961015146139 1101 16-17 -0.051205829219149734 1101 18-19 0.019673216179050712 1101 20-21 0.020633321098941337 1101 22-23 0.0366183039979191 1101 24-25 -0.03504312899378448 1101 26-27 -0.0255688177543405 1101 28-29 -0.05989471068033936 1101 30-31 0.051576721241836765 1101 32-33 0.10239755465847367 1101 34-35 -0.08614768800942585 1101 36-37 0.09343181512046783 1101 38-39 -0.10305505612546995 1101 40-41 -0.044379261676688486 1101 42-43 -0.17213551203997923 1101 44-45 -0.011406614747130561 1101 46-47 -0.042131556668572046 1101 48-49 0.11386069358201922 1101 50-51 0.24769007285338063 1101 52-53 0.24679926603685942 1101 54-55 0.29309444615037705 1101 56-57 0.21385299580087747 1101 58-59 0.28591743436502526 1101 60-61 0.2774727063964093 1101 62-63 0.31780127538668523 1101 64-65 0.28539725384001713 1101 66-67 0.3602745936005789 1101 68-69 0.3858746485228224 1101 70-71 0.24802612399959045 1101 72-73 0.23002347628361974 1101 74-75 0.22944586287635715 1101 76-77 0.07886026123536638 1101 78-79 0.25363260216851913 1101 80-81 0.4514697320504446 1101 82-83 0.47040898190316227 1101 84-85 0.5127201206394645 1101 86-87 0.5755420699428129 1101 88-89 0.7086195041672241 1101 90-91 0.5423269078309652 1101 92-93 0.837786829077487 1101 94-95 0.7879598473565288 1101 96-97 0.6689476470076379 1101 98-99 0.9557233919891281 1101 100-101 1.5130741061650532 1102 1 -0.014609252125680428 1102 2 0.0052015171926456105 1102 3 -0.025424775933061028 1102 4 -0.07031598150943097 1102 5 -0.17882167490751044 1102 6 -0.15719403263688747 1102 7 8.566263439533373E-4 1102 8 0.024119838084061485 1102 9 0.010462667293275274 1102 10-11 -0.1584417171120478 1102 12-13 -0.12847730203355923 1102 14-15 -0.15792832710328497 1102 16-17 -0.22573432278175432 1102 18-19 0.03150357160588868 1102 20-21 -0.07778915174407075 1102 22-23 -0.15502093386009363 1102 24-25 -0.2484052145277289 1102 26-27 -0.3167496458133314 1102 28-29 -0.4340209150494019 1102 30-31 -0.2864549281438613 1102 32-33 -0.08701443098959061 1102 34-35 -0.1172073131325746 1102 36-37 0.02303010799973748 1102 38-39 0.14698805021043881 1102 40-41 0.04737188791712299 1102 42-43 0.30738870383655126 1102 44-45 0.3608448758534877 1102 46-47 0.370095706523891 1102 48-49 0.28781260558140076 1102 50-51 0.4550787733922874 1102 52-53 0.24704966717289523 1102 54-55 0.39580778407439254 1102 56-57 0.3849939353981924 1102 58-59 0.45435228449225207 1102 60-61 0.3530449773260429 1102 62-63 0.4141804529946782 1102 64-65 0.25387636346628994 1102 66-67 0.5047576964660223 1102 68-69 0.5241356126330814 1102 70-71 0.34835296337714894 1102 72-73 0.3329718398353201 1102 74-75 0.27360157723894574 1102 76-77 0.3287594967431282 1102 78-79 0.4980883585941136 1102 80-81 0.6703922603551113 1102 82-83 0.6543939622279993 1102 84-85 0.6654230800953478 1102 86-87 0.8735809238524226 1102 88-89 0.676556077998768 1102 90-91 0.6692281168950487 1102 92-93 1.0217987165419444 1102 94-95 0.6711504630248335 1102 96-97 0.8924487820496303 1102 98-99 1.226979208598852 1102 100-101 1.5953479022352965 1103 1 -0.06025667758862596 1103 2 -0.10376704484157528 1103 3 -0.09853578501802218 1103 4 -0.07279707189068318 1103 5 -0.3219504458170732 1103 6 -0.13617664645401106 1103 7 -0.2632009535030946 1103 8 -0.2238361608214703 1103 9 -0.10108437371659562 1103 10-11 -0.2103127375126448 1103 12-13 -0.19607426165439534 1103 14-15 -0.039016853335596124 1103 16-17 -0.11017811796890697 1103 18-19 -0.2487158773601692 1103 20-21 -0.3330516140059956 1103 22-23 -0.2845686334023654 1103 24-25 -0.1987254375012384 1103 26-27 -0.38662828107449343 1103 28-29 -0.3822403269150385 1103 30-31 -0.33487792854732135 1103 32-33 -0.36425889027419345 1103 34-35 -0.3239006920994214 1103 36-37 -0.40634692962471775 1103 38-39 -0.378780508113735 1103 40-41 -0.44715116303566305 1103 42-43 -0.3989939591386289 1103 44-45 -0.37435321718508163 1103 46-47 -0.01672019656982826 1103 48-49 0.21675603208315408 1103 50-51 -0.010345956818120783 1103 52-53 -0.11543763752819558 1103 54-55 -0.15235319463459973 1103 56-57 -0.21082252883932284 1103 58-59 -0.36470434465661583 1103 60-61 -0.31526106401309306 1103 62-63 -0.1241929719766901 1103 64-65 -0.09319571800622839 1103 66-67 -7.447591178149082E-4 1103 68-69 -0.15080908455794884 1103 70-71 0.006776381160303657 1103 72-73 0.15808511204571474 1103 74-75 0.037967554301612694 1103 76-77 0.0033100020044294354 1103 78-79 0.18197889381400145 1103 80-81 0.21651735368584468 1103 82-83 0.2521419669212044 1103 84-85 0.11116835790790702 1103 86-87 0.38845587225965517 1103 88-89 0.056731006464524114 1103 90-91 0.24930472925597869 1103 92-93 0.5185361986293984 1103 94-95 0.050734103595988955 1103 96-97 0.1899778942830288 1103 98-99 0.08324987469045908 1103 100-101 0.6187513089810786 1104 1 0.06557029274374315 1104 2 -0.002844794339345924 1104 3 0.039889707427697374 1104 4 -0.025517447120918746 1104 5 -0.12085763611806044 1104 6 -0.07213782225746002 1104 7 -0.006924770688257809 1104 8 -0.07207584045151805 1104 9 -0.030093503908652508 1104 10-11 0.10216885484208404 1104 12-13 0.015596075551684407 1104 14-15 -0.07871142928939179 1104 16-17 -0.12443717453037806 1104 18-19 -0.06813412983174061 1104 20-21 0.07741250042691661 1104 22-23 0.27375997438620914 1104 24-25 0.05990582494452923 1104 26-27 0.2906060224241642 1104 28-29 0.20244363304750834 1104 30-31 0.3016188822594472 1104 32-33 0.3295291064025392 1104 34-35 0.278966724500215 1104 36-37 0.16738145925025094 1104 38-39 -0.1453199147611457 1104 40-41 -0.22991896954768265 1104 42-43 0.1473075877629597 1104 44-45 0.2522551164779401 1104 46-47 0.35075135125488544 1104 48-49 0.40987475974671383 1104 50-51 0.4751675010937646 1104 52-53 0.21106233829459597 1104 54-55 0.1729027540569703 1104 56-57 0.3784792774177319 1104 58-59 0.38567040203220415 1104 60-61 0.33000767661823716 1104 62-63 0.33590474789301794 1104 64-65 0.2321580384721642 1104 66-67 0.4134349522503058 1104 68-69 0.26861158563419707 1104 70-71 0.24840557315809164 1104 72-73 0.32190326429177674 1104 74-75 0.5903441736249171 1104 76-77 0.2600972030313038 1104 78-79 0.45820566721203804 1104 80-81 0.4387058743485639 1104 82-83 0.45468760689183796 1104 84-85 0.5690417748504117 1104 86-87 0.6778855979143046 1104 88-89 0.9043629590596183 1104 90-91 0.978245284100872 1104 92-93 1.3122606751928956 1104 94-95 1.3119721084050582 1104 96-97 1.5806210958725693 1104 98-99 1.3162609918092407 1104 100-101 1.7813001579262924 1105 1 0.012306638842851214 1105 2 -0.007904722867856151 1105 3 -0.1648002138271636 1105 4 -0.07249572105279611 1105 5 -0.019508848919677746 1105 6 -0.05349514808523281 1105 7 0.05856967920264111 1105 8 0.1321476186421222 1105 9 0.13348449805206997 1105 10-11 -0.22603866547668616 1105 12-13 -0.2148480912994586 1105 14-15 -0.04147351777489661 1105 16-17 -0.015458963847599705 1105 18-19 -0.1719614913952583 1105 20-21 0.1912529861800607 1105 22-23 0.11590206350420118 1105 24-25 0.055308405508078806 1105 26-27 0.12976749739333115 1105 28-29 0.08649616143054573 1105 30-31 -0.10700592404015907 1105 32-33 0.053624644178064784 1105 34-35 -0.12423447090790063 1105 36-37 -0.06093990946041572 1105 38-39 -0.059415768782209 1105 40-41 0.22948301014935168 1105 42-43 0.0945499267149188 1105 44-45 -0.2590783328120878 1105 46-47 -0.16238074450425444 1105 48-49 -0.039213662504323565 1105 50-51 0.1483791326044681 1105 52-53 -0.0011028367473073786 1105 54-55 0.0142463232040555 1105 56-57 0.35586772180595716 1105 58-59 0.21408743501661576 1105 60-61 0.19565471497882214 1105 62-63 0.24231188239223656 1105 64-65 -0.11960977405747997 1105 66-67 -0.00811639021198829 1105 68-69 0.13264577177631054 1105 70-71 0.28437454947129837 1105 72-73 0.31891474400462627 1105 74-75 0.3117595305080023 1105 76-77 0.1830756034613934 1105 78-79 0.3790770817783091 1105 80-81 0.6085060071716875 1105 82-83 0.2079449358820682 1105 84-85 0.23322854946222193 1105 86-87 0.29393980239866835 1105 88-89 0.225644702204999 1105 90-91 0.1247895110386672 1105 92-93 0.36627541223473514 1105 94-95 -0.023426044721031047 1105 96-97 0.1487878205175832 1105 98-99 0.39264219417475843 1105 100-101 1.0580782484357663 1106 1 -0.43449756827688546 1106 2 -0.20821486310518367 1106 3 -0.3381753486707453 1106 4 -0.06838299181870866 1106 5 -0.21594223619044328 1106 6 -0.3689279959816787 1106 7 -0.2665044847671538 1106 8 -0.22094029937297677 1106 9 -0.1982644231992765 1106 10-11 -0.46179581218650867 1106 12-13 -0.4300415918388296 1106 14-15 -0.3393429891880473 1106 16-17 -0.39494251293066185 1106 18-19 -0.1927948247285869 1106 20-21 -0.2300657840464737 1106 22-23 -0.5527872568841516 1106 24-25 -0.5479649224315111 1106 26-27 -0.704779428173012 1106 28-29 -0.3607462873288938 1106 30-31 -0.6277886910304531 1106 32-33 -0.5737519739449155 1106 34-35 -0.9513818549424187 1106 36-37 -0.7159088954366837 1106 38-39 -0.24639324990410216 1106 40-41 -0.4571742174666156 1106 42-43 -0.4759732663810894 1106 44-45 -0.41716557660928544 1106 46-47 -0.3656574435333866 1106 48-49 -0.01421703359385873 1106 50-51 -0.5691909860578832 1106 52-53 -0.8236655390126728 1106 54-55 -0.6554880349404968 1106 56-57 -0.9969505090462505 1106 58-59 -0.7784118907654758 1106 60-61 -0.45877489667166316 1106 62-63 -0.29433186086557583 1106 64-65 -0.47187177513622913 1106 66-67 -0.06520579150648587 1106 68-69 -0.5662316554081528 1106 70-71 -0.4652209197843682 1106 72-73 -0.05301756451748574 1106 74-75 -0.251330410160822 1106 76-77 -0.09460104163029825 1106 78-79 0.4238114399228614 1106 80-81 0.17456250663231287 1106 82-83 0.0030838247709574773 1106 84-85 -0.484240436514046 1106 86-87 -0.2945175870295955 1106 88-89 -0.021843836090578606 1106 90-91 -0.35462998734320905 1106 92-93 -0.24003189628737687 1106 94-95 0.03462411709126556 1106 96-97 -0.03345327980604296 1106 98-99 -0.22312102049622595 1106 100-101 0.4758068083063165 1107 1 -0.2562080945926759 1107 2 -0.24600726076599955 1107 3 -0.1417206703081746 1107 4 -0.03716954152631047 1107 5 -0.43747001396822327 1107 6 -0.12430080299922253 1107 7 -0.20031296429932155 1107 8 -0.024375971887600656 1107 9 0.022532360426453124 1107 10-11 -0.024752683531538366 1107 12-13 -0.14087858014292465 1107 14-15 -0.019313749421968396 1107 16-17 0.06971391981787178 1107 18-19 -0.07854043876367456 1107 20-21 -0.16098683667805602 1107 22-23 0.05861625188777708 1107 24-25 0.20113960973223044 1107 26-27 -0.0039022351905515507 1107 28-29 -0.54148459142246 1107 30-31 -0.2201680769953711 1107 32-33 -0.34617521955895114 1107 34-35 -0.1399600713166862 1107 36-37 -0.13590158549516218 1107 38-39 -0.14396269434855213 1107 40-41 0.3130377708374823 1107 42-43 0.2861612365428883 1107 44-45 0.1843512362562123 1107 46-47 0.20649167927363266 1107 48-49 -0.1968741973365482 1107 50-51 -0.2542056059409177 1107 52-53 -0.35376422322320167 1107 54-55 -0.5248228302621385 1107 56-57 -0.0571113277597064 1107 58-59 -0.09385414222746391 1107 60-61 0.1257645770125464 1107 62-63 0.05124562451454295 1107 64-65 0.06780293246610825 1107 66-67 0.29669113831807437 1107 68-69 -0.054992620320433616 1107 70-71 -0.14679986715279014 1107 72-73 0.35228214308485306 1107 74-75 0.4273062857456118 1107 76-77 -0.029213614729716397 1107 78-79 0.3251637791041482 1107 80-81 0.08817727271418718 1107 82-83 0.3368923043031202 1107 84-85 0.0670388032520357 1107 86-87 0.2736110679411681 1107 88-89 0.07440981888018072 1107 90-91 0.18007396002521148 1107 92-93 0.3417480744728536 1107 94-95 0.16773815217898758 1107 96-97 0.41062566756238184 1107 98-99 0.9377707923692746 1107 100-101 1.6654449662010542 1108 1 -0.24541528979587568 1108 2 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48-49 0.9075915123923224 2316 50-51 1.2193191149522207 2316 52-53 1.1298083445598834 2316 54-55 1.3754961490042135 2316 56-57 1.231883887551298 2316 58-59 1.5737694622382392 2316 60-61 1.0386832372996295 2316 62-63 0.9308309514682875 2316 64-65 0.7566386581439417 2316 66-67 1.0848889054632096 2316 68-69 0.9625512393286826 2316 70-71 0.924332509242749 2316 72-73 1.016875444520256 2316 74-75 1.1450096350279075 2316 76-77 1.4774849498635874 2316 78-79 0.9006023755953763 2316 80-81 1.212898165856128 2316 82-83 1.2378492421021647 2316 84-85 1.373099409312644 2316 86-87 1.2117290902697135 2316 88-89 1.0980142845899081 2316 90-91 1.3349383938370103 2316 92-93 0.7125614716020436 2316 94-95 0.746845010233212 2316 96-97 1.0047245511349487 2316 98-99 1.049573025224138 2316 100-101 0.5877735148453915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 11.0 6 25.0 7 42.0 8 151.0 9 289.0 10 459.0 11 673.0 12 1078.0 13 1489.0 14 1978.0 15 2640.0 16 3744.0 17 5235.0 18 4824.0 19 3053.0 20 2525.0 21 2619.0 22 2688.0 23 3008.0 24 3299.0 25 3727.0 26 4614.0 27 5486.0 28 6751.0 29 8356.0 30 10261.0 31 12862.0 32 16028.0 33 20614.0 34 27836.0 35 40166.0 36 59992.0 37 89395.0 38 68282.0 39 5341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44378894354929 20.00872764732674 15.880502769716495 21.666980639407473 2 15.674838217592868 21.097856010678235 40.33274142225506 22.894564349473832 3 18.994148422696018 25.041175559236795 31.252189872361726 24.712486145705466 4 12.85034978369424 18.22736536009248 37.196248316628726 31.726036539584552 5 13.188369177079709 37.62258465909903 34.63549984982336 14.553546313997892 6 30.631755830721374 37.464395952758345 17.212933058432352 14.690915158087927 7 26.98399456554124 33.93795659583596 21.064963233979668 18.013085604643127 8 25.977904634782924 36.02807803692095 19.5237697982338 18.470247530062327 9 25.64420979871051 17.13546818577268 20.952222050078063 36.26809996543875 10-11 24.439928970670607 27.587386335196463 27.183973113730353 20.78871158040258 12-13 25.505727385735348 24.960818269472576 28.256135888796585 21.277318455995488 14-15 22.38699066846226 26.327926682477447 27.031307726227222 24.253774922833067 16-17 21.020748668200074 29.57036789855677 27.92489482653827 21.48398860670488 18-19 20.846512293079407 27.30040877617419 31.274833450523783 20.578245480222623 20-21 21.554779582643103 26.44042951292472 31.787531730803607 20.217259173628573 22-23 21.37945335758194 26.242840423985147 31.71736866066172 20.660337557771193 24-25 21.26387672285883 26.618638159423657 31.69583534445259 20.421649773264924 26-27 21.114968542665537 26.793377930168173 31.60976458544131 20.481888941724975 28-29 20.825606506266475 26.983867815242625 31.760477100783252 20.430048577707645 30-31 21.03996119601139 26.646517830647287 31.678165248263294 20.635355725078032 32-33 20.712676828468936 26.865652075462705 31.629741744032223 20.791929352036135 34-35 21.24718444982064 26.805229474788163 31.3612365777211 20.586349497670096 36-37 21.350033965367242 26.644936776746235 31.305700222860477 20.69932903502604 38-39 21.197964461499957 26.96373452192256 31.21929709566316 20.619003920914324 40-41 20.9989393271282 26.98208773790654 31.3612365777211 20.657736357244158 42-43 21.090446199981884 26.94136801605729 31.102889644289128 20.865296139671702 44-45 21.267802023620828 27.239509468590974 30.633543481628905 20.859145026159293 46-47 21.280553933427882 27.558903097403135 30.124897210072817 21.035645759096163 48-49 21.653696266193137 27.57451524866224 29.813011715072278 20.95877677007234 50-51 21.609481700413543 27.886043213481273 29.498861862255538 21.005613223849647 52-53 21.66847418036206 28.105805098380387 29.243108605751466 20.982612115506083 54-55 21.578614928076846 28.127376085997923 29.291971063890642 21.002037922034585 56-57 21.506036301231095 28.312815073472454 29.207951471236697 20.973197154059754 58-59 21.59863661824119 27.87317212694705 29.438558438308167 21.089632816503592 60-61 21.516404676494773 27.872933773492715 29.71326079443206 20.89740075558045 62-63 21.280315579973543 28.02822104899355 29.716240212611282 20.975223158421624 64-65 21.35944892681357 27.787603236839907 29.7946584990883 21.05828933725822 66-67 21.295093494142463 28.034418238806325 29.459176012108358 21.211312254942854 68-69 21.4172496394904 27.900701950923022 29.411862851422377 21.2701855581642 70-71 21.379708970432254 28.027625165357712 29.264441239914667 21.328224624295366 72-73 21.35408597409098 27.989011905755046 29.2273772777652 21.429524842388776 74-75 21.363500935537306 28.174093363048065 28.9081028256802 21.554302875734425 76-77 21.406404557318044 27.99127626357125 29.041819113563506 21.5605000655472 78-79 21.397823832961897 28.136075987081245 28.854711651908616 21.61138852804824 80-81 21.283771705061437 28.323064272008963 28.947073615464376 21.446090407465228 82-83 21.31182903342677 28.221354613580456 28.912462101710428 21.554354251282344 84-85 21.117068630383667 28.183422735848048 28.99692404500595 21.702584588762335 86-87 21.274237566887937 27.899271830196998 28.857452716633496 21.969037886281566 88-89 21.20690271603761 27.895577351654772 28.812403913763717 22.0851160185439 90-91 21.226636600057684 27.861591893457415 29.001961798561666 21.909809707923234 92-93 21.259532841600194 27.747526138965263 29.00379347208169 21.989147547352854 94-95 20.916349855200274 27.880441907304345 28.994386776150353 22.20882146134503 96-97 20.981897055143072 27.97494905194913 28.824321586480593 22.2188323064272 98-99 20.994987402959886 28.27993793246461 28.4682380590309 22.256836605544606 100-101 20.889205148192914 28.23830059095991 28.634856384107255 22.237637876739925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 290.0 1 219.5 2 179.5 3 221.0 4 186.0 5 157.5 6 253.0 7 595.0 8 966.5 9 1115.5 10 1047.5 11 931.0 12 924.5 13 956.0 14 1048.5 15 1106.0 16 1104.5 17 1148.5 18 1219.5 19 1219.0 20 1269.5 21 1360.5 22 1584.5 23 1910.5 24 2217.5 25 2695.0 26 3309.0 27 3965.5 28 4537.5 29 5171.0 30 6083.0 31 6835.5 32 7300.0 33 7837.0 34 8640.5 35 9570.0 36 10604.5 37 11478.5 38 12134.0 39 12470.5 40 12794.5 41 13522.5 42 14376.0 43 15173.5 44 15729.0 45 15970.5 46 15867.0 47 15874.5 48 16335.5 49 16111.0 50 15381.0 51 14509.0 52 13501.5 53 12758.5 54 11895.0 55 10968.5 56 9510.5 57 8155.0 58 7363.0 59 6437.5 60 5300.0 61 4170.0 62 3208.0 63 2398.5 64 1704.0 65 1227.5 66 903.0 67 730.5 68 565.5 69 360.5 70 255.5 71 204.5 72 158.5 73 117.5 74 93.0 75 62.5 76 41.0 77 34.5 78 21.0 79 15.5 80 10.0 81 4.5 82 4.0 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04481044941543815 2 0.0 3 0.0 4 0.0 5 0.009295784719160042 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0060780130856046425 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.767069086748739E-4 24-25 5.958836358435925E-4 26-27 0.0020260043618682143 28-29 0.0026218879977118067 30-31 1.1917672716871848E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011202612353859538 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3835345433743696E-4 84-85 1.1917672716871848E-4 86-87 0.0 88-89 0.0 90-91 0.007627310538797983 92-93 0.0020260043618682143 94-95 0.0 96-97 0.0 98-99 4.767069086748739E-4 100-101 0.013824500351571343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 419545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.80872316993046 #Duplication Level Percentage of deduplicated Percentage of total 1 86.71333328988987 62.26773745355616 2 7.990413732326259 11.475628154356542 3 2.1879400554194164 4.713395452560455 4 1.001391045714513 2.8763444954624253 5 0.5311987342215294 1.907235142696563 6 0.34689784309475136 1.4946174709822184 7 0.2416705885829447 1.214783947570678 8 0.17014498650464494 0.9774315387730881 9 0.13298885273106556 0.8594783739406562 >10 0.6496862876246015 7.858708397143241 >50 0.024000874201682183 1.166934254714459 >100 0.008666982319529306 1.0290564814244128 >500 6.666909476561005E-4 0.3047194465813625 >1k 0.0010000364214841507 1.8539293902377474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2949 0.7029043368411017 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.6917017244872421 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.45144144251510565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.17876509075307773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.12465885661847956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.191767271687185E-4 0.0 0.0 1.191767271687185E-4 0.0 20-21 2.38353454337437E-4 0.0 0.0 2.38353454337437E-4 0.0 22-23 2.38353454337437E-4 0.0 0.0 7.15060363012311E-4 0.0 24-25 2.38353454337437E-4 0.0 0.0 9.53413817349748E-4 0.0 26-27 2.38353454337437E-4 0.0 0.0 0.0010725905445184663 0.0 28-29 2.38353454337437E-4 0.0 0.0 0.0032177716335553995 0.0 30-31 2.38353454337437E-4 0.0 0.0 0.0066738967214482366 0.0 32-33 2.38353454337437E-4 0.0 0.0 0.011440965808196975 0.0 34-35 2.38353454337437E-4 0.0 0.0 0.015492974531933403 0.0 36-37 2.38353454337437E-4 0.0 0.0 0.020855927254525737 0.0 38-39 2.38353454337437E-4 0.0 0.0 0.028959944701998593 0.0 40-41 2.38353454337437E-4 0.0 0.0 0.03408454397025349 0.0 42-43 3.575301815061555E-4 0.0 0.0 0.04624057014146278 0.0 44-45 9.53413817349748E-4 0.0 0.0 0.06197189812773362 0.0 46-47 0.001430120726024622 0.0 0.0 0.07829910974984805 0.0 48-49 0.001430120726024622 0.0 0.0 0.104994696635641 0.0 50-51 0.001430120726024622 0.0 0.0 0.13538476206366423 0.0 52-53 0.001430120726024622 0.0 0.0 0.18770334529073163 0.0 54-55 0.001430120726024622 0.0 0.0 0.23751921724725594 0.0 56-57 0.001430120726024622 0.0 0.0 0.3201086891751779 0.0 58-59 0.001430120726024622 0.0 0.0 0.4285595108987117 0.0 60-61 0.001430120726024622 0.0 0.0 0.5351035049875461 0.0 62-63 0.001430120726024622 0.0 0.0 0.6683430859621733 0.0 64-65 0.001430120726024622 0.0 0.0 0.7695241273284152 0.0 66-67 0.001430120726024622 0.0 0.0 0.9497193388075177 0.0 68-69 0.001668474180362059 0.0 0.0 1.162926503712355 0.0 70-71 0.001668474180362059 0.0 0.0 1.360283163903753 0.0 72-73 0.001668474180362059 0.0 0.0 1.6287883302148756 0.0 74-75 0.001668474180362059 0.0 0.0 1.8983660870705168 0.0 76-77 0.001668474180362059 0.0 0.0 2.126112812689938 0.0 78-79 0.001668474180362059 0.0 0.0 2.388420789188287 0.0 80-81 0.001668474180362059 0.0 0.0 2.775745152486622 0.0 82-83 0.001668474180362059 0.0 0.0 3.1194508336412063 0.0 84-85 0.001668474180362059 0.0 0.0 3.5866236041425834 0.0 86-87 0.001668474180362059 0.0 0.0 4.06678663790535 0.0 88-89 0.001668474180362059 0.0 0.0 4.735606430776198 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1320 0.0 46.08078 1 GTATCAA 2235 0.0 39.3349 1 CAGTCCG 65 1.3270507E-4 36.53704 9 ATCAACG 2885 0.0 30.458088 3 TATCAAC 2920 0.0 30.255674 2 TCAACGC 2930 0.0 29.990301 4 AACGCAG 3015 0.0 29.302343 6 CAACGCA 3000 0.0 29.290527 5 TGTCGAG 70 0.008387194 27.141802 2 TGGTATC 665 0.0 27.141802 2 GTGGTAT 685 0.0 27.055641 1 ACGCAGA 3400 0.0 25.844584 7 CGCAGAG 3395 0.0 25.742739 8 GCAGAGT 3610 0.0 23.420141 9 AGAGTAC 3190 0.0 23.07904 10-11 GAGTACT 2555 0.0 22.494232 12-13 TACCTGG 320 0.0 22.26476 2 AGTACTT 2680 0.0 22.065372 12-13 CGCAGCG 55 0.0028733965 21.595217 26-27 CGTCTAT 155 3.8999882E-5 21.450779 6 >>END_MODULE