FastQCFastQC Report
Thu 26 May 2016
SRR937879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005157
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130430.4340205852805694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108240.3601808491203621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68240.22707632246834358No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40430.13453540031352773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72950.048.31181
GTATCAA124000.041.3866271
ATCAACG157700.032.2284623
TATCAAC160950.031.6072252
TCAACGC161450.031.4814684
CAACGCA163650.030.9736775
AACGCAG170700.029.862536
CGCAGAG196100.025.5572158
ACGCAGA197600.025.4851137
GCAGAGT207550.023.3229169
GTACATG121250.022.9082221
GAGTACT118500.022.3283912-13
TACATGG122950.022.2347812
ACATGGG125700.020.9540543
GTACTTT126900.020.79198314-15
GTGGTAT40650.020.5926571
AGTACTT125300.020.54796212-13
TGGTATC38350.020.2076512
CAGAGTA205000.019.71951710-11
AGAGTAC198550.019.53470610-11
Produced by FastQC (version 0.11.5)