FastQCFastQC Report
Thu 26 May 2016
SRR937879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005157
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153910.5121529424253042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135690.4515238305353098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87850.2923308166594957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.11007744354121932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.048.863171
GTATCAA141450.039.640591
ATCAACG178150.031.1763083
TCAACGC180550.030.7618924
TATCAAC182500.030.5113032
CAACGCA184050.030.1769075
AACGCAG192150.029.0449476
CGCAGAG219800.025.0246778
ACGCAGA220950.025.0019177
GCAGAGT234300.023.1313489
GAGTACT136550.023.1148412-13
GTACTTT145500.021.52905514-15
GTATAGG9100.021.4093531
TGGTATC41950.021.0657542
AGTACTT143650.020.93079212-13
TACATGG128600.020.8369482
GTACATG131300.020.809621
CAGAGTA230550.020.25237710-11
AGAGTAC221600.020.05218110-11
ACATGGG128800.019.9561793
Produced by FastQC (version 0.11.5)