##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3005157 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.034404858049015 31.0 31.0 34.0 30.0 34.0 2 32.32920409815527 33.0 31.0 34.0 31.0 34.0 3 32.41323098926279 34.0 31.0 34.0 31.0 34.0 4 36.07602564524915 37.0 35.0 37.0 35.0 37.0 5 35.97447986910501 37.0 35.0 37.0 35.0 37.0 6 36.007121424937196 37.0 35.0 37.0 35.0 37.0 7 35.948792692029066 37.0 35.0 37.0 35.0 37.0 8 35.922655288891725 37.0 35.0 37.0 35.0 37.0 9 37.59865957086435 39.0 37.0 39.0 35.0 39.0 10-11 37.51030395416945 39.0 37.0 39.0 35.0 39.0 12-13 37.396218400569424 39.0 37.0 39.0 34.0 39.0 14-15 38.58940664331348 40.0 38.0 41.0 34.0 41.0 16-17 38.54050670231206 40.0 38.0 41.0 34.0 41.0 18-19 38.524935968403646 40.0 38.0 41.0 34.0 41.0 20-21 38.42247243654824 40.0 38.0 41.0 34.0 41.0 22-23 38.36630066249451 40.0 38.0 41.0 34.0 41.0 24-25 38.395695133399016 40.0 38.0 41.0 34.0 41.0 26-27 38.2529510105462 40.0 38.0 41.0 34.0 41.0 28-29 38.09445696181597 40.0 38.0 41.0 33.0 41.0 30-31 38.13431145194744 40.0 37.5 41.0 34.0 41.0 32-33 38.05404459733718 40.0 37.0 41.0 33.5 41.0 34-35 37.91315878005708 40.0 37.0 41.0 33.0 41.0 36-37 37.755246065347 40.0 37.0 41.0 33.0 41.0 38-39 37.62032632571277 40.0 37.0 41.0 33.0 41.0 40-41 37.43386701593295 40.0 37.0 41.0 32.5 41.0 42-43 37.20692945493363 40.0 36.0 41.0 32.0 41.0 44-45 36.90582804825172 39.5 36.0 41.0 31.0 41.0 46-47 36.530457144169176 39.0 36.0 41.0 30.5 41.0 48-49 36.18692567476508 39.0 35.5 41.0 30.0 41.0 50-51 35.920407818959205 39.0 35.0 40.5 29.0 41.0 52-53 35.667436842734006 39.0 35.0 40.0 28.0 41.0 54-55 35.43314725320508 38.5 35.0 40.0 28.0 41.0 56-57 35.152846423664386 38.0 34.5 40.0 27.0 41.0 58-59 34.88742385173221 38.0 34.0 40.0 26.0 41.0 60-61 34.552475627729265 38.0 34.0 40.0 26.0 41.0 62-63 34.157492769928496 37.0 33.5 40.0 25.5 41.0 64-65 33.72950381627316 37.0 33.0 39.5 24.5 41.0 66-67 33.29022427114457 36.0 32.5 39.0 23.0 40.0 68-69 32.82548016626086 36.0 32.0 39.0 22.0 40.0 70-71 32.41207763853935 35.0 31.5 38.0 21.5 40.0 72-73 31.904618960007745 35.0 31.0 37.5 20.0 39.5 74-75 31.40727456169511 35.0 31.0 37.0 18.5 39.0 76-77 30.190263603532195 33.5 29.5 35.5 18.0 38.0 78-79 30.788453149036805 34.0 30.5 36.0 18.0 38.0 80-81 30.597108736748197 34.0 30.5 36.0 17.5 37.0 82-83 30.211564320932318 34.0 30.0 35.0 13.0 37.0 84-85 29.80232014500407 34.0 30.0 35.0 7.5 36.5 86-87 29.395845042372162 34.0 29.0 35.0 4.5 36.0 88-89 28.924455693995355 34.0 29.0 35.0 2.0 36.0 90-91 28.33449400480574 33.5 28.5 35.0 2.0 35.0 92-93 27.835149378218844 33.0 27.0 35.0 2.0 35.0 94-95 27.307721859456926 33.0 26.0 35.0 2.0 35.0 96-97 26.671565412389437 32.5 25.5 34.0 2.0 35.0 98-99 25.691279856593184 32.0 24.0 34.0 2.0 35.0 100-101 22.864825864339203 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2753497766659052 1101 2 0.20649640447706474 1101 3 0.24030218153953342 1101 4 0.09111844391669166 1101 5 0.033326026136649034 1101 6 0.050913516561415406 1101 7 0.14969094252045778 1101 8 0.06582407088922082 1101 9 0.06815396038164323 1101 10-11 0.15325195736335928 1101 12-13 0.16998270202264365 1101 14-15 0.23358400182761585 1101 16-17 0.20570202137921711 1101 18-19 0.1857872475159894 1101 20-21 0.22485071970997694 1101 22-23 0.16505536894052852 1101 24-25 0.1860069130528359 1101 26-27 0.0804671648148485 1101 28-29 -0.013538095843344422 1101 30-31 0.16004992733736145 1101 32-33 0.18236196102901658 1101 34-35 0.229607841835751 1101 36-37 0.11529319133892812 1101 38-39 0.18522256087592837 1101 40-41 0.21605291811801663 1101 42-43 0.3390701401871965 1101 44-45 0.2420379837921729 1101 46-47 0.2137817841811085 1101 48-49 0.30146521611950305 1101 50-51 0.3080503797776899 1101 52-53 0.2712917336643912 1101 54-55 0.1346375415570833 1101 56-57 0.23470102891626254 1101 58-59 0.22233483138173682 1101 60-61 0.14026960171728575 1101 62-63 0.0289462075664062 1101 64-65 0.109030174109634 1101 66-67 0.07515159147687456 1101 68-69 0.14231896382013787 1101 70-71 0.08656094092163613 1101 72-73 -0.07261669635946433 1101 74-75 -0.00992640335300976 1101 76-77 0.02227431360955734 1101 78-79 -0.022790697695263873 1101 80-81 -0.10922486317316427 1101 82-83 -0.07973125927766844 1101 84-85 -0.016476617252074277 1101 86-87 -0.0998137681425213 1101 88-89 -0.025337925656060634 1101 90-91 -0.027240369559496713 1101 92-93 -0.13597407690852137 1101 94-95 -0.18823577991945584 1101 96-97 -0.34068707184110636 1101 98-99 -0.3912401275995272 1101 100-101 -0.4064408117261351 1102 1 0.23925695047858397 1102 2 0.15368019526444243 1102 3 0.15865445342063111 1102 4 0.05724127889070729 1102 5 0.015285011646057 1102 6 0.03389041798664749 1102 7 0.013070542502589433 1102 8 3.293354580051755E-4 1102 9 0.12029217951280913 1102 10-11 0.10167984691910448 1102 12-13 0.18503009803436754 1102 14-15 0.1775275138236836 1102 16-17 0.11815678480870417 1102 18-19 0.20237417619485853 1102 20-21 0.24285282702676625 1102 22-23 0.21733413791010747 1102 24-25 0.09149412767150977 1102 26-27 2.2642434989705862E-4 1102 28-29 -0.0051912970288015 1102 30-31 0.11141558775405258 1102 32-33 0.07840273723644486 1102 34-35 0.1417280780339425 1102 36-37 0.10062537297027774 1102 38-39 0.20114769528252907 1102 40-41 0.3328401535864671 1102 42-43 0.31869022745840425 1102 44-45 0.32106106002789403 1102 46-47 0.09173835707341738 1102 48-49 0.15971223385670896 1102 50-51 0.2170973215621288 1102 52-53 0.26682485502498565 1102 54-55 0.19673506639065863 1102 56-57 0.2396744454501345 1102 58-59 0.10024635386226066 1102 60-61 0.05868851842242151 1102 62-63 0.0644105618934816 1102 64-65 0.11603700241171566 1102 66-67 0.1959615308635918 1102 68-69 0.20216564349308896 1102 70-71 -0.02493417427338329 1102 72-73 -0.08137844297509744 1102 74-75 -0.141193436876474 1102 76-77 -0.061107855362990904 1102 78-79 0.021572043752602355 1102 80-81 0.007637952738193121 1102 82-83 0.032332198508978394 1102 84-85 0.009712564692019754 1102 86-87 -0.1828677392804714 1102 88-89 -0.11861759563387153 1102 90-91 -0.05897577843852275 1102 92-93 -0.1467480131975556 1102 94-95 -0.4852082805523956 1102 96-97 -0.4453916751740934 1102 98-99 -0.6181368988625771 1102 100-101 -0.6823515002155709 1103 1 0.2500303288496397 1103 2 0.16688895233169632 1103 3 0.1837127074261531 1103 4 0.12173975254544445 1103 5 0.09333273477244575 1103 6 0.09369515417277086 1103 7 0.08074543999359918 1103 8 0.12034748903182191 1103 9 0.10812251133484807 1103 10-11 0.1288169560753687 1103 12-13 0.24828740010622852 1103 14-15 0.2519707139103886 1103 16-17 0.23279073289490526 1103 18-19 0.1507450961565624 1103 20-21 0.09896520758347549 1103 22-23 0.02484506618090876 1103 24-25 0.10527955423533797 1103 26-27 0.11986344318987818 1103 28-29 0.08441623354325856 1103 30-31 0.09823392557309774 1103 32-33 0.11073235555981853 1103 34-35 0.19067973172516872 1103 36-37 0.19411085914284598 1103 38-39 0.19204155558129088 1103 40-41 0.05650279515725032 1103 42-43 0.13638409136983398 1103 44-45 0.14777548895800408 1103 46-47 0.0718769022586514 1103 48-49 0.1396216014620606 1103 50-51 0.11410288504544752 1103 52-53 0.0065895359375431894 1103 54-55 0.011922879110805695 1103 56-57 0.11558438204810528 1103 58-59 0.00675319080523451 1103 60-61 0.1007962296270648 1103 62-63 -0.07271394814065957 1103 64-65 -0.04144457306560412 1103 66-67 0.15551609093294871 1103 68-69 0.20837488826828832 1103 70-71 0.22336855689115254 1103 72-73 0.12486737989660313 1103 74-75 0.1167483000863676 1103 76-77 0.12830504360920614 1103 78-79 0.222919111182744 1103 80-81 0.08318775313919602 1103 82-83 -0.014951087558831233 1103 84-85 0.05974191748549984 1103 86-87 0.10174552637585421 1103 88-89 0.047898092305292295 1103 90-91 0.057403757697024105 1103 92-93 0.03806327091941952 1103 94-95 -0.13670233774066887 1103 96-97 -0.1952751671630395 1103 98-99 -0.1129665171859493 1103 100-101 -0.11584619864948209 1104 1 0.2085242273010124 1104 2 0.180338162444734 1104 3 0.15595344165844693 1104 4 0.08503792541746691 1104 5 -0.021817701878667606 1104 6 0.03838972083602954 1104 7 0.06687448229737214 1104 8 0.04193224561495157 1104 9 -0.005312017691437632 1104 10-11 0.0562814879044069 1104 12-13 0.12272851782629601 1104 14-15 0.09475607197319391 1104 16-17 0.12384839397534364 1104 18-19 0.17978962433758028 1104 20-21 0.14140355814113548 1104 22-23 0.09102251158283536 1104 24-25 0.020746746455252207 1104 26-27 0.08770363288731176 1104 28-29 -0.04802896818821978 1104 30-31 0.07861554485248234 1104 32-33 0.07184415870868577 1104 34-35 0.13184060529432173 1104 36-37 0.08764228334172941 1104 38-39 0.029615572727017536 1104 40-41 -0.03880682006481351 1104 42-43 0.05935198427797417 1104 44-45 0.06061854814058165 1104 46-47 -0.06378879604945809 1104 48-49 -0.10393985223245039 1104 50-51 -0.08867562184354227 1104 52-53 -0.060064155021130716 1104 54-55 -0.1755187858134093 1104 56-57 -0.10996982897694352 1104 58-59 -0.06968410983228068 1104 60-61 -0.11907684570470423 1104 62-63 -0.17338393955445497 1104 64-65 -0.18653575151198254 1104 66-67 -0.18429123092541033 1104 68-69 -0.272702970428476 1104 70-71 -0.3995735328948129 1104 72-73 -0.21873767217676843 1104 74-75 -0.383294630970358 1104 76-77 -0.3039446327395616 1104 78-79 -0.2825402511242352 1104 80-81 -0.36831794801615914 1104 82-83 -0.4605854662151998 1104 84-85 -0.3826732992384798 1104 86-87 -0.2619130004438226 1104 88-89 -0.3464812758741189 1104 90-91 -0.38417285755618025 1104 92-93 -0.20783047360297857 1104 94-95 -0.3688212189528848 1104 96-97 -0.22705482629489637 1104 98-99 -0.13490918086894155 1104 100-101 -0.35382016418897067 1105 1 0.22550556188240023 1105 2 0.1929471950751278 1105 3 0.12254755919683191 1105 4 0.07689594260932608 1105 5 -0.03985234775299773 1105 6 0.062367266382302944 1105 7 0.06627494028940362 1105 8 0.02862874715757613 1105 9 0.16049279817482187 1105 10-11 0.13212988603197573 1105 12-13 0.19298630769364422 1105 14-15 0.14293334110553957 1105 16-17 0.12712256194035376 1105 18-19 0.14359963242237228 1105 20-21 -0.030896677364950165 1105 22-23 -0.0055714036156189195 1105 24-25 -0.06291553588636845 1105 26-27 -0.035618199327139166 1105 28-29 -0.08704564891604605 1105 30-31 -0.02013742536571783 1105 32-33 0.08968688955874882 1105 34-35 0.12501594339994426 1105 36-37 0.10768194196787562 1105 38-39 0.034400868068537704 1105 40-41 0.11310480841092385 1105 42-43 0.08315270232080252 1105 44-45 0.14902644960280043 1105 46-47 0.03218481530480233 1105 48-49 0.04144719326148305 1105 50-51 0.13979162679968482 1105 52-53 0.22211897845020445 1105 54-55 0.1139407554379801 1105 56-57 0.23333905348202677 1105 58-59 0.21645578095694162 1105 60-61 0.11182120928072692 1105 62-63 0.09556101606712275 1105 64-65 0.16773863550799462 1105 66-67 0.07342108744151687 1105 68-69 0.2372188119927685 1105 70-71 0.11793071844305558 1105 72-73 0.14016480085703265 1105 74-75 0.03445616717268862 1105 76-77 0.034764799204282326 1105 78-79 0.24743627744396335 1105 80-81 0.06450730682218264 1105 82-83 0.14772784572874897 1105 84-85 0.2555854425062698 1105 86-87 0.1130274632313224 1105 88-89 0.14354230992372763 1105 90-91 0.3539536594434516 1105 92-93 0.2385532719906287 1105 94-95 0.09477329710718863 1105 96-97 0.012842209073241406 1105 98-99 0.06429003686729473 1105 100-101 0.013530396776491926 1106 1 0.0738707866126802 1106 2 0.06779740391876743 1106 3 0.1057762948428902 1106 4 0.006271468658169965 1106 5 -0.02543166294787369 1106 6 -0.0510381055573248 1106 7 -0.072654577436289 1106 8 -0.07354561725929898 1106 9 -0.001035312612984285 1106 10-11 -0.18623296378907384 1106 12-13 -0.4130053293450189 1106 14-15 -0.09831149655416738 1106 16-17 -0.07438977694343407 1106 18-19 -0.09950444491064303 1106 20-21 0.07709004010038711 1106 22-23 0.07886058251351358 1106 24-25 -0.10892785868860244 1106 26-27 0.01984275101808919 1106 28-29 0.12333145109766974 1106 30-31 0.15175268997082014 1106 32-33 0.09784350933946939 1106 34-35 -0.3554149703797904 1106 36-37 0.10915854201574149 1106 38-39 0.0171254078112284 1106 40-41 -0.12780093423388195 1106 42-43 -0.22189480461891975 1106 44-45 -0.09610897229286763 1106 46-47 -0.08016074071656476 1106 48-49 -0.09225683296076426 1106 50-51 0.06173733639298007 1106 52-53 0.24581425059296436 1106 54-55 0.12267870728934582 1106 56-57 0.11398917405682596 1106 58-59 0.16340807753656605 1106 60-61 0.2493483601517923 1106 62-63 0.052535208443615034 1106 64-65 -0.3213952101188511 1106 66-67 -0.29633541570414934 1106 68-69 0.07909224951306726 1106 70-71 0.00821825203114912 1106 72-73 0.0681817811355181 1106 74-75 0.011406783713692192 1106 76-77 0.006185111967941026 1106 78-79 -0.1273187322828413 1106 80-81 -0.1088833235388158 1106 82-83 -0.16182291120560421 1106 84-85 -0.36532282710831865 1106 86-87 0.012447154113434777 1106 88-89 0.05037066702197279 1106 90-91 -0.16991666664162963 1106 92-93 -0.18429023992230142 1106 94-95 -0.27507531786546835 1106 96-97 -0.11462327986453857 1106 98-99 -0.09367418946109396 1106 100-101 -0.4089166979040506 1107 1 0.12794335075595598 1107 2 0.09480368201720779 1107 3 0.012673406932520948 1107 4 -0.0437851288987261 1107 5 -0.014298791191194482 1107 6 -0.028935706655808247 1107 7 0.04356879450339335 1107 8 0.01691943687085029 1107 9 0.03327432391776597 1107 10-11 0.07841917834553414 1107 12-13 0.04426090615896072 1107 14-15 0.03469756091853782 1107 16-17 0.030973880529714393 1107 18-19 0.15744156806449183 1107 20-21 0.10645507775629426 1107 22-23 -0.040164200768344926 1107 24-25 -0.01252085311494966 1107 26-27 -0.004942813753594066 1107 28-29 0.018119211191120144 1107 30-31 0.0704408976266393 1107 32-33 -0.017908045462696975 1107 34-35 0.04003064080300334 1107 36-37 0.12114666677859987 1107 38-39 0.05601572856674153 1107 40-41 -0.00283853238041587 1107 42-43 0.06899287690511358 1107 44-45 0.05394439191660183 1107 46-47 -0.053441643504427816 1107 48-49 0.013638370056185067 1107 50-51 0.2004349887777508 1107 52-53 0.21518303595013322 1107 54-55 0.12960720241677848 1107 56-57 0.07984339462814205 1107 58-59 0.06285036703726377 1107 60-61 0.15861592589403983 1107 62-63 0.062312962864268684 1107 64-65 0.19412019850544482 1107 66-67 0.11657166925890294 1107 68-69 0.20013377501197027 1107 70-71 0.1992051889348474 1107 72-73 0.33830135535491834 1107 74-75 0.17940550565624847 1107 76-77 0.14605785784956993 1107 78-79 0.11156763763893807 1107 80-81 0.24576419872935062 1107 82-83 0.3054429869866304 1107 84-85 0.37873866910969056 1107 86-87 0.2105667902043713 1107 88-89 0.2628427782357079 1107 90-91 0.3161343744030596 1107 92-93 0.38297880775531823 1107 94-95 0.3172353067464364 1107 96-97 0.35494239491923096 1107 98-99 0.35329378791330157 1107 100-101 0.5551029940203698 1108 1 0.06467478587323683 1108 2 -0.014639161777715515 1108 3 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2316 80-81 -0.15108921922912444 2316 82-83 -0.06369022838103078 2316 84-85 -0.04654039170632629 2316 86-87 -0.08433666347610114 2316 88-89 -0.044435648445578124 2316 90-91 0.2004470166680008 2316 92-93 0.33083611082740916 2316 94-95 0.46236666124713466 2316 96-97 0.5920592833945904 2316 98-99 0.5740979759716289 2316 100-101 0.7730469990934452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 20.0 5 57.0 6 113.0 7 326.0 8 801.0 9 1512.0 10 2696.0 11 4328.0 12 6732.0 13 9433.0 14 12334.0 15 17043.0 16 22947.0 17 31527.0 18 29170.0 19 18498.0 20 15947.0 21 16385.0 22 17131.0 23 19371.0 24 22454.0 25 26682.0 26 32449.0 27 39896.0 28 49944.0 29 61672.0 30 75721.0 31 94907.0 32 119782.0 33 155115.0 34 209778.0 35 297017.0 36 446615.0 37 650664.0 38 461174.0 39 34905.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.93154262078257 20.083078891331798 15.729341605943393 22.25603688194224 2 15.531834110497389 20.659918932688043 40.2080823065151 23.60016465029947 3 18.982735344609285 24.70390066142967 30.42267009677032 25.89069389719073 4 12.943021926315973 17.475798261388096 36.93202615771028 32.649153654585646 5 13.343939163943638 37.81128412440979 34.37847036359424 14.466306348052335 6 31.564008136679718 36.87234976408887 17.155276745940395 14.408365353291025 7 28.0373038746395 33.41762177483572 20.51150738547104 18.03356696505374 8 26.196900860753697 35.87150355206067 19.24495126211376 18.686644325071867 9 26.098137301977896 16.970061797104112 20.607242816265504 36.32455808465248 10-11 24.702186940649025 27.428383941338176 26.88067545223095 20.988753665781854 12-13 25.56293722350636 24.602201899341175 27.96290367988201 21.871957197270454 14-15 22.62625546685248 25.885103507071346 26.42630651243845 25.062334513637722 16-17 21.42009552246355 29.328983477402343 27.176849662097517 22.074071338036582 18-19 21.42019535085854 27.474388193362277 30.05285580753352 21.052560648245667 20-21 22.18246833692882 26.470796700471887 30.414567358710375 20.932167603888914 22-23 22.032500981651438 26.290222751685445 30.28516142342786 21.39211484323526 24-25 21.760524140138738 26.625388645260863 30.307583596894734 21.30650361770567 26-27 21.428068715358098 26.78051453160879 30.360952127892332 21.43046462514078 28-29 21.45442602243064 26.90029601043948 30.402007500173454 21.243270466956428 30-31 21.592000018634632 26.715010230746678 30.32933054745559 21.363659203163095 32-33 21.4759994236574 26.727006276211192 30.266904524455796 21.530089775675616 34-35 21.813136551601133 26.733744692872953 30.12353431118574 21.32958444434018 36-37 21.927340235468563 26.468350904794658 30.078361962453208 21.525946897283568 38-39 21.596109620895014 26.80249318088872 30.12110515357434 21.480292044641928 40-41 21.876124116803993 26.873217427358554 29.910599749695272 21.34005870614218 42-43 21.694443020812464 26.784873973071395 29.810997878751373 21.70968512736477 44-45 21.785567276518332 27.001118410785192 29.731824327314683 21.481489985381796 46-47 22.007252865657268 27.251421473154313 29.077366007832534 21.66395965335588 48-49 22.031078575927978 27.33046892391978 28.908938867420236 21.729513632732 50-51 22.226575849448132 27.532538233443375 28.616907535945707 21.623978381162782 52-53 22.22601015520986 27.580755348223075 28.496930443234746 21.696304053332323 54-55 22.210912021632172 27.55719466576333 28.49999176414782 21.731901548456676 56-57 22.160040224187956 27.839410719639606 28.384573584674612 21.615975471497826 58-59 22.139658593544365 27.517464145800037 28.49922649631949 21.843650764336108 60-61 22.17880796244589 27.60556270437784 28.56423141952317 21.6513979136531 62-63 21.99720347389504 27.57471573032624 28.766683404560894 21.66139739121783 64-65 22.074221080629066 27.44453950326056 28.687536125400438 21.79370329070994 66-67 21.991529893446497 27.535316790437236 28.547526801428347 21.925626514687917 68-69 22.022942561736375 27.53190598694178 28.5900703357595 21.855081115562346 70-71 22.092705971767863 27.53045847521444 28.46382069223006 21.913014860787637 72-73 22.100342843984524 27.446253224041207 28.33437654006097 22.119027391913303 74-75 22.006886828208973 27.875365579901484 28.02073236107132 22.097015230818222 76-77 22.12741297709238 27.69306229258571 28.241153457207062 21.93837127311485 78-79 22.129792220506282 27.685691629422355 28.152056614679367 22.032459535391993 80-81 21.98081497905101 27.90243571300934 28.105985810391935 22.010763497547718 82-83 22.243935049861303 27.913561981887007 27.9213652892914 21.921137678960285 84-85 22.12669194655651 27.846928386900444 27.95254688151745 22.073832785025598 86-87 22.079861384945946 27.782691553220012 28.048135255495804 22.089311806338237 88-89 22.150639716993155 27.68843691028455 27.977406837646086 22.183516535076205 90-91 22.13087789122002 27.770288855346354 28.02990606556125 22.068927187872372 92-93 22.0228041910675 27.67498512115465 28.11187494082986 22.190335746947987 94-95 21.93296390171961 27.689435194234445 28.015108694820274 22.362492209225675 96-97 22.03166090823208 27.67086711276649 27.913000884812337 22.38447109418909 98-99 21.87168845922894 27.64218213908916 27.88050908525812 22.605620316423778 100-101 21.998274322771536 27.42074490346941 28.231313969249 22.349666804510058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 888.0 1 622.0 2 439.0 3 571.0 4 502.5 5 460.0 6 899.0 7 2073.0 8 3525.0 9 4369.5 10 4175.5 11 3891.5 12 4158.5 13 4450.5 14 4724.5 15 4982.0 16 5206.0 17 5444.5 18 5688.5 19 6066.0 20 6608.5 21 7643.5 22 9078.5 23 10719.0 24 13057.0 25 16215.5 26 20630.0 27 25178.0 28 28291.0 29 31948.5 30 37239.5 31 42879.5 32 48998.0 33 54853.5 34 61163.5 35 67336.5 36 75001.0 37 83093.5 38 86564.0 39 88797.0 40 92090.5 41 97755.5 42 101466.0 43 104658.0 44 111046.5 45 113955.0 46 114412.5 47 116671.0 48 119300.0 49 117719.0 50 112398.5 51 104826.5 52 98535.0 53 97012.5 54 95234.5 55 87500.0 56 77053.5 57 68291.0 58 61036.0 59 53637.0 60 43980.0 61 34967.5 62 27664.5 63 20373.0 64 14655.0 65 10890.5 66 7934.0 67 6070.0 68 4671.5 69 3387.5 70 2413.5 71 1611.0 72 1102.0 73 841.0 74 685.5 75 518.0 76 327.0 77 188.0 78 111.5 79 71.0 80 39.5 81 24.5 82 18.5 83 13.0 84 7.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018068939493011513 2 0.0 3 0.0 4 3.327613166300463E-5 5 0.03823427528079232 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003826755141245532 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.656942382710787E-4 24-25 2.1629485580953008E-4 26-27 0.0011813026740366643 28-29 8.818174890696227E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.655226332600926E-4 42-43 0.010748190527150495 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.1646646082051619E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.987987649230971E-4 84-85 4.991419749450694E-5 86-87 0.0 88-89 0.0 90-91 0.011596731884557112 92-93 0.0017137207806447383 94-95 0.0 96-97 0.0 98-99 0.001380959464014692 100-101 0.017952473032190996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3005157.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.463563281408874 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92473423505747 33.66340611582756 2 15.095197723768864 14.329437448149651 3 5.601763844986953 7.976390182321396 4 2.716767040934339 5.157897774729317 5 1.4871336714816636 3.5292331562141928 6 0.9281655225913094 2.6432425810280726 7 0.6120604942865426 2.0335400401833796 8 0.4309668387274604 1.636417745770364 9 0.3179146269508904 1.3580424912932174 >10 1.6751652164862967 14.613656380812124 >50 0.13549168601389236 4.433086627051559 >100 0.0704931725144442 5.899640146558663 >500 0.003302686972950656 1.0255262167193544 >1k 6.324294202715435E-4 0.44158862716166747 >5k 7.026993558572706E-5 0.2930027258017559 >10k+ 1.405398711714541E-4 0.9658917403777861 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15391 0.5121529424253042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13569 0.4515238305353098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8785 0.2923308166594957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3308 0.11007744354121932 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.982839498901389E-5 0.0 10-11 0.0 0.0 0.0 9.982839498901389E-5 0.0 12-13 0.0 0.0 0.0 9.982839498901389E-5 0.0 14-15 0.0 0.0 0.0 1.331045266520185E-4 1.6638065831502314E-5 16-17 0.0 0.0 0.0 1.6638065831502314E-4 3.327613166300463E-5 18-19 0.0 0.0 0.0 1.9965678997802778E-4 3.327613166300463E-5 20-21 0.0 0.0 0.0 1.9965678997802778E-4 3.327613166300463E-5 22-23 0.0 0.0 0.0 2.8284711913553935E-4 3.327613166300463E-5 24-25 0.0 0.0 0.0 3.9931357995605555E-4 3.327613166300463E-5 26-27 0.0 0.0 0.0 6.488845674285902E-4 3.327613166300463E-5 28-29 0.0 0.0 0.0 0.0010981123448791526 3.327613166300463E-5 30-31 0.0 0.0 0.0 0.0021795866239268033 3.327613166300463E-5 32-33 0.0 0.0 0.0 0.0034440796271209793 6.655226332600925E-5 34-35 0.0 0.0 0.0 0.00445900164284262 6.655226332600925E-5 36-37 0.0 0.0 0.0 0.006372379213465387 6.655226332600925E-5 38-39 0.0 0.0 0.0 0.008518689705729185 6.655226332600925E-5 40-41 0.0 0.0 0.0 0.010731552461318992 6.655226332600925E-5 42-43 0.0 0.0 0.0 0.014042527561787954 6.655226332600925E-5 44-45 0.0 0.0 0.0 0.02006550739279179 6.655226332600925E-5 46-47 0.0 0.0 0.0 0.03176206767233792 6.655226332600925E-5 48-49 0.0 0.0 0.0 0.05327508679247041 6.655226332600925E-5 50-51 0.0 0.0 0.0 0.07084488431053684 6.655226332600925E-5 52-53 0.0 0.0 0.0 0.08743303594454466 6.655226332600925E-5 54-55 0.0 0.0 0.0 0.11055994745033287 6.655226332600925E-5 56-57 0.0 0.0 0.0 0.12981018961738106 6.655226332600925E-5 58-59 0.0 0.0 0.0 0.154517717377162 6.655226332600925E-5 60-61 0.0 0.0 0.0 0.19310139204041585 6.655226332600925E-5 62-63 0.0 0.0 0.0 0.2243643177378087 6.655226332600925E-5 64-65 0.0 0.0 0.0 0.26128418581791235 6.655226332600925E-5 66-67 0.0 0.0 0.0 0.31717144894592864 6.655226332600925E-5 68-69 0.0 0.0 0.0 0.38184361083297813 6.655226332600925E-5 70-71 0.0 0.0 0.0 0.4409919348639688 6.655226332600925E-5 72-73 0.0 0.0 0.0 0.5028522636254944 8.319032915751157E-5 74-75 0.0 0.0 0.0 0.5686724520549176 9.982839498901389E-5 76-77 0.0 0.0 0.0 0.6326624532428755 9.982839498901389E-5 78-79 0.0 0.0 0.0 0.7097965264377202 9.982839498901389E-5 80-81 0.0 0.0 0.0 0.8082106858310565 9.982839498901389E-5 82-83 0.0 0.0 0.0 0.9256754306014627 1.331045266520185E-4 84-85 0.0 0.0 0.0 1.0556852770088219 1.331045266520185E-4 86-87 0.0 0.0 0.0 1.2354429402523728 1.331045266520185E-4 88-89 0.0 0.0 0.0 1.4613379600466798 1.331045266520185E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8480 0.0 48.86317 1 GTATCAA 14145 0.0 39.64059 1 ATCAACG 17815 0.0 31.176308 3 TCAACGC 18055 0.0 30.761892 4 TATCAAC 18250 0.0 30.511303 2 CAACGCA 18405 0.0 30.176907 5 AACGCAG 19215 0.0 29.044947 6 CGCAGAG 21980 0.0 25.024677 8 ACGCAGA 22095 0.0 25.001917 7 GCAGAGT 23430 0.0 23.131348 9 GAGTACT 13655 0.0 23.11484 12-13 GTACTTT 14550 0.0 21.529055 14-15 GTATAGG 910 0.0 21.409353 1 TGGTATC 4195 0.0 21.065754 2 AGTACTT 14365 0.0 20.930792 12-13 TACATGG 12860 0.0 20.836948 2 GTACATG 13130 0.0 20.80962 1 CAGAGTA 23055 0.0 20.252377 10-11 AGAGTAC 22160 0.0 20.052181 10-11 ACATGGG 12880 0.0 19.956179 3 >>END_MODULE