FastQCFastQC Report
Thu 26 May 2016
SRR937878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858258
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124750.4364546517494222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103900.3635081227796791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.22877570884083945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39030.13655170386997956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.052.501951
GTATCAA114800.042.2076841
ATCAACG150650.031.8660723
TCAACGC152200.031.606784
TATCAAC151950.031.5627172
CAACGCA155300.031.005935
AACGCAG161200.029.912236
CGCAGAG184550.025.5613028
ACGCAGA186050.025.508427
GTACATG119150.024.064811
TACATGG118350.023.4546242
GCAGAGT195850.023.2617729
TGGTATC32850.022.7168542
ACATGGG120100.022.0439033
GAGTACT114400.021.98655912-13
AGTACTT120500.020.89325712-13
GTACTTT121950.020.4502514-15
GGACCGA5600.020.3596826
AGAGTAC187000.020.34754810-11
CAGAGTA192100.020.17826810-11
Produced by FastQC (version 0.11.5)