FastQCFastQC Report
Thu 26 May 2016
SRR937878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858258
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145960.5106606891330314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130980.4582511445782711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.3016172787760937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.11482518373078987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.050.397531
GTATCAA129800.039.5392071
ATCAACG169750.029.9206353
TATCAAC173450.029.5015132
TCAACGC172850.029.4664824
CAACGCA176450.028.8114415
AACGCAG183550.027.6877656
CGCAGAG207850.024.132958
ACGCAGA210750.023.8910267
CCGTCCG3800.022.5001499
GAGTACT133550.022.4252712-13
GTACATG118700.022.138761
GCAGAGT222400.022.0415139
TACATGG118100.021.6036572
AGTACTT137600.021.4890612-13
GTACTTT141750.020.90836314-15
ACATGGG117950.020.5838133
AGAGTAC208450.020.09847810-11
GTATAGG9300.019.9278551
CAGAGTA218350.019.7201910-11
Produced by FastQC (version 0.11.5)