##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2858258 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11097318716505 33.0 31.0 34.0 30.0 34.0 2 32.330960676048136 34.0 31.0 34.0 31.0 34.0 3 32.41087333613691 34.0 31.0 34.0 31.0 34.0 4 36.060291268317975 37.0 35.0 37.0 35.0 37.0 5 35.96003579802803 37.0 35.0 37.0 35.0 37.0 6 35.98899854386833 37.0 35.0 37.0 35.0 37.0 7 35.93124413541395 37.0 35.0 37.0 35.0 37.0 8 35.907292833607045 37.0 35.0 37.0 35.0 37.0 9 37.56900496736124 39.0 37.0 39.0 35.0 39.0 10-11 37.49771189304814 39.0 37.0 39.0 35.0 39.0 12-13 37.38945609528601 39.0 37.0 39.0 34.0 39.0 14-15 38.57939433739011 40.0 38.0 41.0 34.0 41.0 16-17 38.53590228733725 40.0 38.0 41.0 34.0 41.0 18-19 38.528735859394075 40.0 38.0 41.0 34.0 41.0 20-21 38.425305728174294 40.0 38.0 41.0 34.0 41.0 22-23 38.260578996017856 40.0 38.0 41.0 34.0 41.0 24-25 38.339656881918984 40.0 38.0 41.0 34.0 41.0 26-27 38.210340704023224 40.0 38.0 41.0 34.0 41.0 28-29 38.06103962623388 40.0 37.5 41.0 33.0 41.0 30-31 38.10263594119215 40.0 37.0 41.0 34.0 41.0 32-33 38.02453679828763 40.0 37.0 41.0 33.0 41.0 34-35 37.8889076843308 40.0 37.0 41.0 33.0 41.0 36-37 37.721007690698315 40.0 37.0 41.0 33.0 41.0 38-39 37.576372566787185 40.0 37.0 41.0 33.0 41.0 40-41 37.391079286754376 40.0 37.0 41.0 32.5 41.0 42-43 37.16989613953674 40.0 36.0 41.0 31.5 41.0 44-45 36.8515387694183 39.5 36.0 41.0 31.0 41.0 46-47 36.49572764250114 39.0 36.0 41.0 30.0 41.0 48-49 36.150881236053564 39.0 35.5 41.0 29.5 41.0 50-51 35.89473361047183 39.0 35.0 40.0 29.0 41.0 52-53 35.63238745417664 39.0 35.0 40.0 28.0 41.0 54-55 35.399267665830024 38.5 35.0 40.0 27.5 41.0 56-57 35.128078535947424 38.0 34.5 40.0 27.0 41.0 58-59 34.85738253859519 38.0 34.0 40.0 26.0 41.0 60-61 34.49506202729074 38.0 34.0 40.0 26.0 41.0 62-63 34.13102631043104 37.0 33.5 40.0 25.5 41.0 64-65 33.733052439632814 37.0 33.0 39.5 24.5 41.0 66-67 33.28112455208732 36.0 32.5 39.0 23.0 40.0 68-69 32.82260418758558 36.0 32.0 39.0 22.0 40.0 70-71 32.39477297010977 35.0 31.5 38.0 21.5 40.0 72-73 31.87512621323897 35.0 31.0 37.5 20.0 39.5 74-75 31.348485511105014 35.0 31.0 37.0 18.0 39.0 76-77 30.118400788172377 33.5 29.5 35.5 18.0 38.0 78-79 30.725953535335158 34.0 30.0 36.0 18.0 38.0 80-81 30.530423775600383 34.0 30.0 36.0 16.0 37.5 82-83 30.155154818074507 34.0 30.0 35.0 11.5 37.0 84-85 29.737933209668267 34.0 30.0 35.0 7.0 36.5 86-87 29.288052723022204 34.0 29.0 35.0 4.5 36.0 88-89 28.876849815517005 34.0 29.0 35.0 2.0 36.0 90-91 28.3044548462735 33.5 28.5 35.0 2.0 35.0 92-93 27.774873891720063 33.0 27.0 35.0 2.0 35.0 94-95 27.247958546779195 33.0 26.0 35.0 2.0 35.0 96-97 26.628606479890898 32.5 25.0 34.5 2.0 35.0 98-99 25.66451331545298 32.0 24.0 34.0 2.0 35.0 100-101 22.980364788623 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29526209216378874 1101 2 0.2451612096881277 1101 3 0.18059643690662597 1101 4 0.12917766280666854 1101 5 0.08362462770313783 1101 6 0.19357408112986718 1101 7 0.1823559691589125 1101 8 0.17204410248944413 1101 9 0.2147867779027024 1101 10-11 0.263208226816694 1101 12-13 0.2740755030333517 1101 14-15 0.2550953887754801 1101 16-17 0.2615307437377439 1101 18-19 0.2888985457994053 1101 20-21 0.30225068915127906 1101 22-23 0.30075822439142996 1101 24-25 0.23531337842126732 1101 26-27 0.21339892860459742 1101 28-29 0.25213819339217025 1101 30-31 0.25687097648361856 1101 32-33 0.3866966757354149 1101 34-35 0.3066458186394385 1101 36-37 0.32212422561242704 1101 38-39 0.2754481970462592 1101 40-41 0.33273194901951797 1101 42-43 0.4137858316571794 1101 44-45 0.37457728397029655 1101 46-47 0.4679622764371274 1101 48-49 0.39069613963784633 1101 50-51 0.40316266955917257 1101 52-53 0.27641626762353866 1101 54-55 0.44197055715905975 1101 56-57 0.44886987372655085 1101 58-59 0.47969963993664066 1101 60-61 0.41199011931199436 1101 62-63 0.3561601780339032 1101 64-65 0.44662560197512846 1101 66-67 0.42588164227114333 1101 68-69 0.5202925090628341 1101 70-71 0.5495673670484749 1101 72-73 0.4625803599163696 1101 74-75 0.32352049853422926 1101 76-77 0.22940942932681807 1101 78-79 0.4751886945302992 1101 80-81 0.47736298330831417 1101 82-83 0.6332490755952342 1101 84-85 0.5496877355968088 1101 86-87 0.4172296561600426 1101 88-89 0.7522180725006784 1101 90-91 0.7475786660875912 1101 92-93 0.6205295256127101 1101 94-95 0.681047123703685 1101 96-97 0.866026291182223 1101 98-99 1.0177521099636415 1101 100-101 1.3160292141873455 1102 1 0.19811518127135486 1102 2 0.18870011710014012 1102 3 0.13431865338404236 1102 4 0.0503309159673222 1102 5 0.005823870692687194 1102 6 0.08428863900166306 1102 7 0.1316432558363303 1102 8 0.14351181402803093 1102 9 0.1643645345023259 1102 10-11 0.16039025867149093 1102 12-13 0.18778446297775986 1102 14-15 0.22662146445976106 1102 16-17 0.23753544081711908 1102 18-19 0.21541012127733694 1102 20-21 0.22750126370973334 1102 22-23 0.22082207560524836 1102 24-25 0.10146287261419218 1102 26-27 0.12794590079568735 1102 28-29 0.15666842490173138 1102 30-31 0.18208987696459644 1102 32-33 0.14923867882270514 1102 34-35 0.10137152578650444 1102 36-37 0.12587448296429926 1102 38-39 0.08214321201226937 1102 40-41 0.14258151578335543 1102 42-43 0.25146973926948135 1102 44-45 0.19673553697723634 1102 46-47 0.12843009324544852 1102 48-49 0.14579589900802148 1102 50-51 0.020698137539007178 1102 52-53 0.07213845056369905 1102 54-55 0.19461133678853315 1102 56-57 0.0732288695837724 1102 58-59 -0.061303943892070833 1102 60-61 -0.13790577544808968 1102 62-63 -0.11884548137846451 1102 64-65 0.11954221623982164 1102 66-67 0.033498419471023055 1102 68-69 0.042450296274516575 1102 70-71 -0.11221646109915895 1102 72-73 -0.039042796815355274 1102 74-75 -0.029358116153414215 1102 76-77 0.06205822995061183 1102 78-79 0.13256078948172956 1102 80-81 0.13094280617569254 1102 82-83 0.1248482718189372 1102 84-85 0.11950285214344092 1102 86-87 0.23403787800528164 1102 88-89 0.2868097983000517 1102 90-91 0.34327988824118805 1102 92-93 0.4409964760418461 1102 94-95 0.4205460956935525 1102 96-97 0.6669838730713593 1102 98-99 0.5311409754074994 1102 100-101 0.8137263690167593 1103 1 0.24350848932563451 1103 2 0.22715479853845721 1103 3 0.1505583746408874 1103 4 0.12109149602058977 1103 5 0.04985243167311637 1103 6 0.09892480032864626 1103 7 0.13946667916631839 1103 8 0.12172852305991455 1103 9 0.16585382905218893 1103 10-11 0.14315576697809007 1103 12-13 0.12431259255719596 1103 14-15 0.19956663227697646 1103 16-17 0.22485900228482336 1103 18-19 0.14637183701045586 1103 20-21 0.11853608067500687 1103 22-23 0.28528929762430977 1103 24-25 0.25551875714714356 1103 26-27 0.117355610134247 1103 28-29 0.14600562311103005 1103 30-31 0.24762616874008359 1103 32-33 0.20416854151648778 1103 34-35 0.17382909872903696 1103 36-37 0.21684586386768245 1103 38-39 0.2322813497369225 1103 40-41 0.2184390202381863 1103 42-43 0.34271158400268575 1103 44-45 0.32449737502260234 1103 46-47 0.45033448942265153 1103 48-49 0.30420365832471674 1103 50-51 0.20238246109779112 1103 52-53 0.19086281796891313 1103 54-55 0.4268321622351934 1103 56-57 0.24264638437433916 1103 58-59 0.2951591581358315 1103 60-61 0.2534841761724138 1103 62-63 0.2765594499147852 1103 64-65 0.14975530597391185 1103 66-67 0.16827327286174665 1103 68-69 0.16975185405676285 1103 70-71 0.28548186935667985 1103 72-73 0.22793008259175807 1103 74-75 0.3563998844349001 1103 76-77 0.25410623571573865 1103 78-79 0.3354764342719747 1103 80-81 0.29008242720445665 1103 82-83 0.46228439988557213 1103 84-85 0.2574612659826059 1103 86-87 0.3970540727535443 1103 88-89 0.38218813025796905 1103 90-91 0.3894419841436374 1103 92-93 0.5217186189379461 1103 94-95 0.6322309378276181 1103 96-97 0.7450599409117338 1103 98-99 0.936851338597954 1103 100-101 1.160955299437365 1104 1 0.1219227991694325 1104 2 0.12365738121179959 1104 3 0.006407166995394675 1104 4 0.016422014508556515 1104 5 0.045707431923773356 1104 6 0.033312994365665816 1104 7 0.03175298494035417 1104 8 0.04590822570644093 1104 9 0.10646507334417521 1104 10-11 0.10895573052012963 1104 12-13 0.13087009035390906 1104 14-15 0.115979944945515 1104 16-17 0.09592647936711529 1104 18-19 0.04148742273286388 1104 20-21 -0.018221774635200916 1104 22-23 0.036885786841416746 1104 24-25 -0.0072794339815160924 1104 26-27 0.026373896467681845 1104 28-29 0.01292977444379062 1104 30-31 0.2033238077219366 1104 32-33 0.15988622444348266 1104 34-35 0.1157946969756054 1104 36-37 0.16341249465494911 1104 38-39 0.08222312538023857 1104 40-41 0.009362963290115545 1104 42-43 0.03364698954807466 1104 44-45 0.03202189845908521 1104 46-47 -0.018477704626349123 1104 48-49 -0.03236818917460482 1104 50-51 0.03751065996826952 1104 52-53 0.003142813644707587 1104 54-55 -0.21114247067647085 1104 56-57 -0.04382793501478233 1104 58-59 0.009060563599646798 1104 60-61 -0.019323961158299596 1104 62-63 -0.049060694329376986 1104 64-65 -0.04735997825231664 1104 66-67 -0.014767725772536267 1104 68-69 -0.0896695710791846 1104 70-71 0.036472590904345736 1104 72-73 -0.03340159659481401 1104 74-75 -0.21918843178648473 1104 76-77 -0.16526278793972793 1104 78-79 0.03467730200299002 1104 80-81 0.12975157083284827 1104 82-83 0.14845406011911777 1104 84-85 0.11716611690807355 1104 86-87 0.0692879929060446 1104 88-89 0.13029274396885526 1104 90-91 0.2922989820047093 1104 92-93 0.30818972248532006 1104 94-95 0.2917589350780254 1104 96-97 0.5180356984869547 1104 98-99 0.6635146137997374 1104 100-101 0.722848050498353 1105 1 0.14407078586307165 1105 2 0.13186033272016573 1105 3 0.07183685939968854 1105 4 0.04503349069356943 1105 5 -0.06467849463644626 1105 6 -0.036914205294316105 1105 7 -0.01620067803995795 1105 8 -0.01470237365509064 1105 9 0.1563835715795605 1105 10-11 0.03735523427609877 1105 12-13 0.08939101812688932 1105 14-15 0.05943641623672846 1105 16-17 0.08739229615875388 1105 18-19 0.08747867335257098 1105 20-21 0.20362042515562706 1105 22-23 0.08804159120229116 1105 24-25 0.12426690748748825 1105 26-27 0.017473988352655567 1105 28-29 0.14260224933180865 1105 30-31 0.15321953955984924 1105 32-33 0.17117061313530968 1105 34-35 0.1178953905313449 1105 36-37 0.13116962829502654 1105 38-39 0.11937812393047409 1105 40-41 0.18869649286322243 1105 42-43 0.3727500569236639 1105 44-45 0.3526122018944591 1105 46-47 0.23385032250936177 1105 48-49 0.3539225100560017 1105 50-51 0.41043217994953096 1105 52-53 0.31737953889227555 1105 54-55 0.3084539438273808 1105 56-57 0.24294232992036058 1105 58-59 0.350648948014495 1105 60-61 0.43904203352666116 1105 62-63 0.3690424330458839 1105 64-65 0.3557334060035089 1105 66-67 0.3598980139093939 1105 68-69 0.32379151075993917 1105 70-71 0.35621285485535026 1105 72-73 0.2553050455985648 1105 74-75 0.2957310475008974 1105 76-77 0.2771899883052633 1105 78-79 0.5065329598712616 1105 80-81 0.27854055090969254 1105 82-83 0.5321275487461818 1105 84-85 0.587588522567394 1105 86-87 0.5982970440468272 1105 88-89 0.5084089056352958 1105 90-91 0.4721587642561005 1105 92-93 0.5720293617612668 1105 94-95 0.48914126632717014 1105 96-97 0.6343763067004282 1105 98-99 0.7862150556740133 1105 100-101 0.6713133935480506 1106 1 -0.030840088927895692 1106 2 0.05011467199572195 1106 3 0.055877514048248145 1106 4 0.031040179189574246 1106 5 -0.0142210909356848 1106 6 -0.003584005431100934 1106 7 -0.0037848078673121677 1106 8 -0.07493122795678175 1106 9 -0.002788446791683441 1106 10-11 -0.11634921048942459 1106 12-13 -0.20597055341181658 1106 14-15 0.035780179562074466 1106 16-17 0.07950422998420947 1106 18-19 0.03761107980604805 1106 20-21 -0.018148735689919704 1106 22-23 -0.02919921374425627 1106 24-25 0.07988706359581954 1106 26-27 0.02213062954351841 1106 28-29 0.12921883355564745 1106 30-31 0.10917223857689606 1106 32-33 0.17481383761539604 1106 34-35 0.09838953906994874 1106 36-37 0.06597258022292607 1106 38-39 0.10973591766230584 1106 40-41 0.0896567008990985 1106 42-43 -0.003604237756491102 1106 44-45 0.15256350794983575 1106 46-47 0.1900480745717772 1106 48-49 0.2577829573002006 1106 50-51 0.40949231986635226 1106 52-53 0.22712754727646 1106 54-55 0.3729138875389211 1106 56-57 0.2964088149241775 1106 58-59 0.20496412088297689 1106 60-61 0.24600704997647682 1106 62-63 0.19303956352312923 1106 64-65 0.22462681485015423 1106 66-67 0.23072895680312655 1106 68-69 0.13103541811332775 1106 70-71 0.24638688368393247 1106 72-73 0.1773673201052013 1106 74-75 0.11293726364211665 1106 76-77 0.2134865559612109 1106 78-79 0.2008157370619017 1106 80-81 0.02237308101611646 1106 82-83 -0.06716928427457347 1106 84-85 -5.472253619132061E-7 1106 86-87 0.16097403119993103 1106 88-89 -0.2435317271996027 1106 90-91 -0.10463874303374254 1106 92-93 0.0192682241976172 1106 94-95 0.22107671310316235 1106 96-97 0.20103611833574675 1106 98-99 0.4089254699263023 1106 100-101 0.5830732616491652 1107 1 0.2730548987358361 1107 2 0.2786031434639753 1107 3 0.2186554251288797 1107 4 0.10039731702973853 1107 5 0.09680195232386524 1107 6 0.15853385351120153 1107 7 0.19020738802861104 1107 8 0.18210868535440738 1107 9 0.3203414325496112 1107 10-11 0.24628163776739598 1107 12-13 0.2305505587156489 1107 14-15 0.32257892364616936 1107 16-17 0.30261540294242906 1107 18-19 0.32817667841234055 1107 20-21 0.3556101775006866 1107 22-23 0.3926071407826015 1107 24-25 0.30459315109743557 1107 26-27 0.4243944469935741 1107 28-29 0.44509839083858793 1107 30-31 0.4635065065188897 1107 32-33 0.40240627028966713 1107 34-35 0.4132035531654097 1107 36-37 0.35057517666482596 1107 38-39 0.45033038276530135 1107 40-41 0.49398456421400283 1107 42-43 0.5544594922414845 1107 44-45 0.6574734513820601 1107 46-47 0.48422163001879426 1107 48-49 0.5833046755834914 1107 50-51 0.5723823372958705 1107 52-53 0.5403498948168917 1107 54-55 0.621517709284447 1107 56-57 0.5417984984198867 1107 58-59 0.42855224063468 1107 60-61 0.5086626336947901 1107 62-63 0.6065941498248222 1107 64-65 0.5887002266954795 1107 66-67 0.5142254609526375 1107 68-69 0.5469839240995711 1107 70-71 0.5799633593973113 1107 72-73 0.6845882580186817 1107 74-75 0.5843925207752036 1107 76-77 0.5488203456369085 1107 78-79 0.6799231025657484 1107 80-81 0.7209698079370277 1107 82-83 0.5838017830509479 1107 84-85 0.6452639567682432 1107 86-87 0.754630131348403 1107 88-89 0.9133834633578424 1107 90-91 0.9361496980252149 1107 92-93 0.7766922628844064 1107 94-95 1.1150682086515893 1107 96-97 1.1053219608010068 1107 98-99 1.3469415194548766 1107 100-101 1.5013267228882796 1108 1 -0.07203525808573374 1108 2 0.010388397154386553 1108 3 -0.08509467675000337 1108 4 -0.06350783252846526 1108 5 -0.17030051626148435 1108 6 -0.15184916315858032 1108 7 -0.15347204586038288 1108 8 -0.13582895038423004 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0.11341540535564576 2316 56-57 0.08974107813732246 2316 58-59 0.2506650240688444 2316 60-61 0.30084105146887197 2316 62-63 0.28379818770380894 2316 64-65 0.20147281101326087 2316 66-67 -0.183654768311861 2316 68-69 0.2077356159523518 2316 70-71 0.4435001345913818 2316 72-73 0.3970351299355208 2316 74-75 0.5457931057989427 2316 76-77 0.43905823214462103 2316 78-79 0.4859361599947114 2316 80-81 0.5340985565590977 2316 82-83 0.41081254899226494 2316 84-85 0.3982632160853683 2316 86-87 0.5261587590308459 2316 88-89 0.6043581267955993 2316 90-91 0.8946237177053931 2316 92-93 0.8650877730731352 2316 94-95 0.6495074307031068 2316 96-97 0.8869622840396403 2316 98-99 0.9666482408626358 2316 100-101 1.7932827188029528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 19.0 5 64.0 6 112.0 7 311.0 8 745.0 9 1512.0 10 2590.0 11 4299.0 12 6366.0 13 9075.0 14 11776.0 15 16212.0 16 22258.0 17 30355.0 18 28072.0 19 17975.0 20 15482.0 21 15956.0 22 16921.0 23 18351.0 24 21016.0 25 25345.0 26 30972.0 27 38735.0 28 47569.0 29 58863.0 30 73188.0 31 91746.0 32 115763.0 33 148911.0 34 201516.0 35 282156.0 36 420340.0 37 606054.0 38 440954.0 39 36664.0 40 7.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.96307687354755 20.087085024252303 15.716771758220954 22.233066343979196 2 15.457142077447173 20.720557766303813 40.31766901378392 23.504631142465097 3 19.018716994756947 24.81151106723046 30.433956626728587 25.735815311284004 4 12.991489891709888 17.484591959829938 36.938747092624574 32.5851710558356 5 13.317088756212012 37.843950574771775 34.37537291313001 14.46358775588621 6 31.54939127258631 36.97108518545212 17.048985780849733 14.43053776111184 7 28.086442861351212 33.38627933517548 20.542267353052104 17.9850104504212 8 26.144175928135247 35.92352404856384 19.261732145943437 18.670567877357467 9 26.032779406197758 17.061440919609076 20.58865924629617 36.317120427896995 10-11 24.694870913484877 27.440039954412786 26.892573534749754 20.972515597352583 12-13 25.539514290627558 24.614632649551744 28.013580115385846 21.832272944434852 14-15 22.58498008227389 25.969104258607867 26.43776384077295 25.008151818345297 16-17 21.407882003653974 29.39566687122016 27.2010434327482 21.995407692377665 18-19 21.41529910875785 27.485902252350908 30.10629201422685 20.992506624664394 20-21 22.165931137077198 26.50593473367345 30.450207783901945 20.87792634534741 22-23 22.010097527328593 26.313800752879978 30.328023568477562 21.348078151313864 24-25 21.775879806448444 26.5885689405412 30.402362419188062 21.233188833822297 26-27 21.438360166270094 26.746852347300315 30.438318532272124 21.37646895415747 28-29 21.429721000011195 26.888001153170098 30.444442811719792 21.237835035098914 30-31 21.577303378491376 26.73345093410042 30.411950215830764 21.277295471577442 32-33 21.449253356414992 26.760390419619224 30.295305742168832 21.495050481796955 34-35 21.769220973054217 26.757434073481118 30.202259558094475 21.271085395370186 36-37 21.934951288512096 26.468831714981643 30.132811663607693 21.46340533289857 38-39 21.556390447386683 26.819690129063034 30.176179203076565 21.44774022047372 40-41 21.829735640088046 26.890186228010315 30.02310684866422 21.256971283237412 42-43 21.639139874270043 26.818086623884213 29.879804037488388 21.662969464357353 44-45 21.775798405882185 27.004682572391996 29.776318302966352 21.443200718759467 46-47 22.00656833637831 27.256934118613508 29.148943167481733 21.587554377526452 48-49 22.025408483069057 27.32442277778983 28.96563221374697 21.684536525394137 50-51 22.214002374873086 27.57464511601122 28.692126463041472 21.519226046074216 52-53 22.16946475790499 27.623241148979556 28.521218168548817 21.68607592456664 54-55 22.19792614942388 27.574120320838773 28.554507675654193 21.673445854083152 56-57 22.12193580845396 27.91194846651352 28.37551753550589 21.590598189526627 58-59 22.14056603707573 27.582639495804788 28.49772483799573 21.779069629123754 60-61 22.165196423835777 27.60466339987503 28.619669742899344 21.610470433389846 62-63 21.981710538376873 27.583059331942746 28.82855571470455 21.606674414975835 64-65 22.057928290588183 27.472345043729433 28.71427281931862 21.755453846363764 66-67 22.010906643137183 27.574295252562926 28.584351027793854 21.83044707650604 68-69 21.943322401836504 27.616575081050343 28.60221159452386 21.837890922589292 70-71 22.05689619341571 27.56542621414862 28.485916946615735 21.891760645819936 72-73 22.068196782795674 27.51002533711093 28.335668088744963 22.086109791348438 74-75 22.000830575826257 27.92288169927277 28.05848877183235 22.017798953068617 76-77 22.102378441694206 27.756031820780347 28.258960527706034 21.882629209819406 78-79 22.103148141280457 27.719978392433433 28.163780176597076 22.013093289689035 80-81 21.963589329225467 27.973181543777315 28.107861595997978 21.95536753099924 82-83 22.24461395687317 27.95443631869386 27.932324480726052 21.868625243706916 84-85 22.08108858426912 27.912080299638465 27.96062408236944 22.046207033722972 86-87 22.06410338045061 27.83835469016443 27.982900773827975 22.114641155556985 88-89 22.104075279418442 27.682647962500234 28.084308694316608 22.128968063764713 90-91 22.121991180749156 27.746227412806896 28.066264222375985 22.065517184067964 92-93 21.98246055058358 27.653576018771346 28.172401686314625 22.19156174433045 94-95 21.874914720784478 27.74747765946951 28.08215703410959 22.295450585636427 96-97 21.98636372223921 27.681668344845008 27.978632439758762 22.35333549315702 98-99 21.872570057988856 27.68084978718409 27.907809104367875 22.538771050459175 100-101 21.98658892383331 27.473162538218943 28.19762309889466 22.342625439053087 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 858.0 1 589.5 2 422.5 3 545.0 4 482.0 5 474.5 6 891.5 7 2035.0 8 3435.5 9 4260.5 10 4110.5 11 3756.0 12 3962.5 13 4321.5 14 4649.5 15 4934.0 16 5051.5 17 5167.0 18 5454.5 19 5830.0 20 6397.0 21 7324.5 22 8573.0 23 10336.0 24 12632.5 25 15565.5 26 19907.0 27 24406.5 28 27423.5 29 30867.5 30 35392.0 31 40429.0 32 46618.5 33 52385.5 34 58389.0 35 64336.5 36 71733.5 37 79499.5 38 82848.0 39 84821.5 40 88018.5 41 93259.0 42 97352.5 43 100374.5 44 105406.5 45 108251.0 46 108650.5 47 111241.0 48 113917.0 49 111859.5 50 106325.5 51 99095.5 52 93151.5 53 91347.5 54 89834.0 55 82738.5 56 72727.0 57 64340.5 58 57341.0 59 50384.0 60 41320.0 61 32805.0 62 25929.5 63 19009.5 64 13657.0 65 10226.5 66 7629.0 67 5917.0 68 4622.0 69 3275.0 70 2229.0 71 1509.0 72 1066.0 73 811.5 74 640.5 75 485.0 76 324.5 77 199.0 78 114.0 79 76.0 80 43.0 81 21.5 82 19.0 83 13.0 84 5.5 85 4.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020676929794301285 2 0.0 3 0.0 4 3.4986344829613E-4 5 0.024105591587603357 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7493172414806502E-5 12-13 0.003796018414013011 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011720425517920356 24-25 5.24795172444195E-5 26-27 5.94767862103421E-4 28-29 0.0017493172414806502 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.224522069036455E-4 40-41 5.073020000293884E-4 42-43 0.016321129863014466 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.224522069036455E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.74658620740325E-5 82-83 0.0021166738621915867 84-85 4.723156551997755E-4 86-87 0.0 88-89 0.0 90-91 0.010688328345446772 92-93 0.002536510000146943 94-95 0.0 96-97 0.0 98-99 0.0012770015862808746 100-101 0.019085051104553892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2858258.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.33199635356685 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39592818111923 34.50707740509375 2 15.0176841766632 14.51669313731009 3 5.489467749360821 7.959508057353901 4 2.6189881365301364 5.063237002592376 5 1.4413272107046229 3.483111074603626 6 0.9052413652356012 2.6251273418198973 7 0.5834595980718915 1.9739837016524973 8 0.43422941107776936 1.6789739450257783 9 0.30389437060669366 1.321903945082899 >10 1.6106825816605816 14.245129156659301 >50 0.12673141237443802 4.205077514099809 >100 0.06853145307422648 5.788920578931004 >500 0.003038544299596928 0.9525898428076593 >1k 5.787703427450623E-4 0.4088622042876076 >5k 7.234629284313279E-5 0.30144540008239673 >10k+ 1.4469258568626558E-4 0.9683596925973663 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14596 0.5106606891330314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13098 0.4582511445782711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8621 0.3016172787760937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3282 0.11482518373078987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.4986344829613004E-5 0.0 8 0.0 0.0 0.0 3.4986344829613004E-5 0.0 9 0.0 0.0 0.0 3.4986344829613004E-5 0.0 10-11 0.0 0.0 0.0 3.4986344829613004E-5 0.0 12-13 0.0 0.0 0.0 3.4986344829613004E-5 0.0 14-15 0.0 0.0 0.0 6.997268965922601E-5 0.0 16-17 0.0 0.0 0.0 6.997268965922601E-5 1.7493172414806502E-5 18-19 0.0 0.0 0.0 1.3994537931845202E-4 3.4986344829613004E-5 20-21 0.0 0.0 0.0 2.2741124139248451E-4 3.4986344829613004E-5 22-23 0.0 0.0 0.0 3.14877103466517E-4 3.4986344829613004E-5 24-25 0.0 0.0 0.0 3.84849793125743E-4 3.4986344829613004E-5 26-27 0.0 0.0 0.0 5.073020000293885E-4 6.997268965922601E-5 28-29 0.0 0.0 0.0 0.0011370562069624225 6.997268965922601E-5 30-31 0.0 0.0 0.0 0.002134167034606393 6.997268965922601E-5 32-33 0.0 0.0 0.0 0.0030263188277615248 6.997268965922601E-5 34-35 0.0 0.0 0.0 0.00460070434509411 6.997268965922601E-5 36-37 0.0 0.0 0.0 0.006909803103848568 6.997268965922601E-5 38-39 0.0 0.0 0.0 0.009253888207432639 6.997268965922601E-5 40-41 0.0 0.0 0.0 0.011807891379994388 6.997268965922601E-5 42-43 0.0 0.0 0.0 0.016618513794066175 6.997268965922601E-5 44-45 0.0 0.0 0.0 0.02305600124271497 6.997268965922601E-5 46-47 0.0 0.0 0.0 0.03729544358836746 6.997268965922601E-5 48-49 0.0 0.0 0.0 0.062450625520859206 6.997268965922601E-5 50-51 0.0 0.0 0.0 0.08248030793581265 6.997268965922601E-5 52-53 0.0 0.0 0.0 0.1030347855232103 6.997268965922601E-5 54-55 0.0 0.0 0.0 0.1299567778695975 6.997268965922601E-5 56-57 0.0 0.0 0.0 0.15164831166395754 6.997268965922601E-5 58-59 0.0 0.0 0.0 0.18115929352773613 6.997268965922601E-5 60-61 0.0 0.0 0.0 0.2252420880130485 6.997268965922601E-5 62-63 0.0 0.0 0.0 0.26015846015300226 1.04959034488839E-4 64-65 0.0 0.0 0.0 0.3016697582933381 1.04959034488839E-4 66-67 0.0 0.0 0.0 0.36405041112453806 1.04959034488839E-4 68-69 0.0 0.0 0.0 0.4344429369217194 1.04959034488839E-4 70-71 0.0 0.0 0.0 0.49972745637377736 1.04959034488839E-4 72-73 0.0 0.0 0.0 0.5716768745158765 1.04959034488839E-4 74-75 0.0 0.0 0.0 0.6396553425198145 1.04959034488839E-4 76-77 0.0 0.0 0.0 0.7088408394203742 1.04959034488839E-4 78-79 0.0 0.0 0.0 0.7872977177007814 1.04959034488839E-4 80-81 0.0 0.0 0.0 0.886606457499638 1.04959034488839E-4 82-83 0.0 0.0 0.0 1.0081665126101282 1.04959034488839E-4 84-85 0.0 0.0 0.0 1.1401175121350138 1.04959034488839E-4 86-87 0.0 0.0 0.0 1.327504375042421 1.04959034488839E-4 88-89 0.0 0.0 0.0 1.5712542394703348 1.04959034488839E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7845 0.0 50.39753 1 GTATCAA 12980 0.0 39.539207 1 ATCAACG 16975 0.0 29.920635 3 TATCAAC 17345 0.0 29.501513 2 TCAACGC 17285 0.0 29.466482 4 CAACGCA 17645 0.0 28.811441 5 AACGCAG 18355 0.0 27.687765 6 CGCAGAG 20785 0.0 24.13295 8 ACGCAGA 21075 0.0 23.891026 7 CCGTCCG 380 0.0 22.500149 9 GAGTACT 13355 0.0 22.42527 12-13 GTACATG 11870 0.0 22.13876 1 GCAGAGT 22240 0.0 22.041513 9 TACATGG 11810 0.0 21.603657 2 AGTACTT 13760 0.0 21.48906 12-13 GTACTTT 14175 0.0 20.908363 14-15 ACATGGG 11795 0.0 20.583813 3 AGAGTAC 20845 0.0 20.098478 10-11 GTATAGG 930 0.0 19.927855 1 CAGAGTA 21835 0.0 19.72019 10-11 >>END_MODULE