FastQCFastQC Report
Thu 26 May 2016
SRR937877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688049
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118070.439240504916391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101160.37633242548777945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.23757007405742978No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38860.14456581706657878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.046.943191
GTATCAA115050.041.325531
ATCAACG146050.032.2471123
TATCAAC146400.032.1116832
TCAACGC147500.031.867454
CAACGCA150150.031.3366725
AACGCAG155700.030.481316
CGCAGAG177050.026.4299938
ACGCAGA179500.026.2015827
GCAGAGT188100.024.1940869
GAGTACT108500.023.57479712-13
GTACATG110750.023.0963631
TATAACG1452.3228167E-522.9476342
TACATGG110300.022.5389692
TGGTATC33900.022.1546672
GTACTTT118000.021.43470214-15
AGTACTT113550.021.39688312-13
ACATGGG112450.020.8778723
GTGGTAT38600.020.5699981
CAGAGTA185050.020.49698410-11
Produced by FastQC (version 0.11.5)