##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937877_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2688049 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499580178783944 31.0 31.0 34.0 30.0 34.0 2 31.635214983060205 33.0 31.0 34.0 30.0 34.0 3 31.645694330720907 33.0 31.0 34.0 30.0 34.0 4 35.2522450297595 37.0 35.0 37.0 33.0 37.0 5 35.23781597731291 37.0 35.0 37.0 33.0 37.0 6 35.273589134721874 37.0 35.0 37.0 33.0 37.0 7 35.206387978790566 37.0 35.0 37.0 32.0 37.0 8 35.182484396675804 37.0 35.0 37.0 32.0 37.0 9 36.773914463612826 39.0 37.0 39.0 33.0 39.0 10-11 36.649684771371355 39.0 37.0 39.0 32.0 39.0 12-13 36.5092753145497 39.0 37.0 39.0 32.0 39.0 14-15 37.61159655199738 40.0 37.0 41.0 32.0 41.0 16-17 37.529618879715365 40.0 37.0 41.0 32.0 41.0 18-19 37.46930078283543 40.0 37.0 41.0 32.0 41.0 20-21 37.384839524874735 40.0 37.0 41.0 32.0 41.0 22-23 37.17758604846861 39.5 37.0 41.0 31.0 41.0 24-25 37.00432320988196 39.0 36.0 41.0 31.0 41.0 26-27 36.94001857853038 39.0 36.0 41.0 31.0 41.0 28-29 36.97760141277186 39.0 36.0 41.0 31.0 41.0 30-31 36.78941194896373 39.0 36.0 41.0 30.0 41.0 32-33 36.5388627588262 39.0 35.5 41.0 30.0 41.0 34-35 36.5534117867643 39.0 36.0 41.0 30.0 41.0 36-37 36.45214372952279 39.0 35.5 41.0 30.0 41.0 38-39 36.312287276013194 39.0 35.0 41.0 30.0 41.0 40-41 36.065987822394604 39.0 35.0 41.0 29.5 41.0 42-43 35.799572478031465 39.0 35.0 40.5 28.0 41.0 44-45 35.55335449614199 39.0 35.0 40.0 27.0 41.0 46-47 35.198462714035344 38.5 35.0 40.0 25.5 41.0 48-49 34.81949026970862 38.0 34.0 40.0 24.0 41.0 50-51 33.8849734510048 37.5 33.5 39.5 23.0 40.5 52-53 33.97702608843812 38.0 33.5 39.5 23.0 40.5 54-55 34.31535474241727 38.0 34.0 40.0 23.0 41.0 56-57 34.10274608089362 38.0 34.0 40.0 22.0 41.0 58-59 33.84861362274275 38.0 33.0 40.0 20.5 41.0 60-61 33.52116981498477 38.0 33.0 40.0 19.5 41.0 62-63 33.139724015447634 37.0 32.5 40.0 18.0 41.0 64-65 32.751623575314284 36.5 32.0 39.5 15.0 41.0 66-67 32.32571541664605 36.0 31.5 39.0 12.0 41.0 68-69 31.825018628752673 35.5 31.0 39.0 8.0 40.0 70-71 31.37098486671932 35.0 31.0 38.5 7.0 40.0 72-73 30.891022447879486 35.0 30.0 37.5 4.0 40.0 74-75 30.418872014609853 35.0 30.0 37.0 2.0 39.0 76-77 29.905806776587777 34.0 29.0 36.5 2.0 39.0 78-79 29.359426111651985 34.0 29.0 36.0 2.0 38.5 80-81 28.814306398432468 34.0 28.5 35.5 2.0 37.0 82-83 28.2679953006809 34.0 27.0 35.0 2.0 37.0 84-85 27.779680541537747 33.5 26.0 35.0 2.0 36.0 86-87 27.2851637005129 33.0 26.0 35.0 2.0 36.0 88-89 26.773840060207235 33.0 25.0 35.0 2.0 35.5 90-91 26.24432460122565 32.5 24.5 35.0 2.0 35.0 92-93 25.639221606451372 32.0 21.5 34.5 2.0 35.0 94-95 24.976658721623004 32.0 19.0 34.0 2.0 35.0 96-97 24.211021636882364 31.0 8.5 34.0 2.0 35.0 98-99 23.236700856271593 31.0 2.0 34.0 2.0 35.0 100-101 20.90933014985962 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.010462371418174854 1101 2 0.005126531059641337 1101 3 -0.031920126932444504 1101 4 -0.02226475409249673 1101 5 -0.09285100134543711 1101 6 -0.09846417720814316 1101 7 0.03996577706320181 1101 8 -0.06346840154321853 1101 9 0.0019059144349427015 1101 10-11 -0.036397161765094665 1101 12-13 0.1518535079311576 1101 14-15 0.24910363018823745 1101 16-17 0.24982420125459015 1101 18-19 0.30580221834109267 1101 20-21 0.24234167633335346 1101 22-23 0.12527211859229936 1101 24-25 0.09965489176461517 1101 26-27 0.20755891009405758 1101 28-29 -0.04610042168556561 1101 30-31 0.07571706320439375 1101 32-33 0.06765721990379348 1101 34-35 0.17272414967474248 1101 36-37 0.09428181399852775 1101 38-39 0.21322348751288445 1101 40-41 0.2379257194103772 1101 42-43 0.2048921235486958 1101 44-45 0.13760647149806005 1101 46-47 0.2718547071049855 1101 48-49 0.09091538857215653 1101 50-51 0.07141368384132107 1101 52-53 0.17802186820957644 1101 54-55 0.2729328863886664 1101 56-57 0.3239580151226775 1101 58-59 0.22676393843008924 1101 60-61 0.22756150855703083 1101 62-63 0.17292163237259928 1101 64-65 0.0736142161469786 1101 66-67 0.16697822327159884 1101 68-69 0.25165828047335737 1101 70-71 0.24464113279173816 1101 72-73 0.13105792972283226 1101 74-75 0.3009129900145844 1101 76-77 0.36726063620051264 1101 78-79 0.37522266501333945 1101 80-81 0.2606511507706415 1101 82-83 0.249742539873278 1101 84-85 0.4224110689149434 1101 86-87 0.5100408797679563 1101 88-89 0.6328654974855894 1101 90-91 0.7544649851618423 1101 92-93 0.734314028808349 1101 94-95 0.836368512417895 1101 96-97 1.0039757903476918 1101 98-99 1.4114694073582577 1101 100-101 1.8571023794251147 1102 1 0.17242051146471127 1102 2 0.1324991091171519 1102 3 0.1578367532353937 1102 4 0.10347879767844859 1102 5 -0.028800649623846653 1102 6 0.029628298829535993 1102 7 0.03239656401453317 1102 8 0.18479355904750605 1102 9 0.2718758844049134 1102 10-11 0.2612847667387541 1102 12-13 0.2698139341107577 1102 14-15 0.29598132980566305 1102 16-17 0.35726383266682404 1102 18-19 0.25597910755907805 1102 20-21 0.27027988851813944 1102 22-23 0.3359368929557007 1102 24-25 0.36067961511810864 1102 26-27 0.35938745096370184 1102 28-29 0.22027211523491985 1102 30-31 0.3301722032759784 1102 32-33 0.3139281483665002 1102 34-35 0.3476367334914343 1102 36-37 0.6261732945201359 1102 38-39 0.49261589361761793 1102 40-41 0.46576314587794343 1102 42-43 0.4680600860317341 1102 44-45 0.5025474398636831 1102 46-47 0.5496468828423673 1102 48-49 0.4771283138261708 1102 50-51 0.3595826747500368 1102 52-53 0.3347806818176977 1102 54-55 0.48313692604475733 1102 56-57 0.5116498165131063 1102 58-59 0.44700541278115935 1102 60-61 0.5793674240615729 1102 62-63 0.7400325789356046 1102 64-65 0.6757039771408486 1102 66-67 0.7985755740470282 1102 68-69 0.6629586219106898 1102 70-71 0.7014966184143425 1102 72-73 0.7008530673261895 1102 74-75 0.8009973209208319 1102 76-77 0.7390612038914881 1102 78-79 0.8752720294462684 1102 80-81 0.9678604148292216 1102 82-83 0.9582085401201006 1102 84-85 0.9423069922355225 1102 86-87 1.0034363300127218 1102 88-89 1.060439646977546 1102 90-91 0.9962622618906245 1102 92-93 1.1799617313053687 1102 94-95 1.31159168079175 1102 96-97 1.4640852148406793 1102 98-99 1.4191496903810048 1102 100-101 1.9080793838267738 1103 1 0.1914304359139649 1103 2 0.1453682244967247 1103 3 0.21412035303363552 1103 4 0.19413484523825986 1103 5 0.1644416063799099 1103 6 0.15692816294948386 1103 7 0.12120019131188542 1103 8 0.14867513338933236 1103 9 0.1784018963702536 1103 10-11 0.19809264455081887 1103 12-13 0.15790585149137826 1103 14-15 0.3442770852143866 1103 16-17 0.18345448835538747 1103 18-19 0.20683450569480755 1103 20-21 0.032416651700927446 1103 22-23 0.26989146916969275 1103 24-25 0.27125914264323825 1103 26-27 0.10194976821220791 1103 28-29 -0.05952106706872229 1103 30-31 0.05656299933204423 1103 32-33 0.2003032562897289 1103 34-35 0.30484205596872727 1103 36-37 0.3210409057057362 1103 38-39 0.3781466425374802 1103 40-41 0.39786207086390135 1103 42-43 0.3845016620967314 1103 44-45 0.22317769354833672 1103 46-47 0.20275013552721077 1103 48-49 0.27832610456371754 1103 50-51 0.36137361691754677 1103 52-53 0.2154391346518878 1103 54-55 0.2605017684723592 1103 56-57 0.2426492259643922 1103 58-59 0.3431128172567668 1103 60-61 0.4722140662805998 1103 62-63 0.4837388374954159 1103 64-65 0.49455459703749227 1103 66-67 0.42324686817438817 1103 68-69 0.5345130214985616 1103 70-71 0.6255938776580479 1103 72-73 0.6142350984906457 1103 74-75 0.6125762040478335 1103 76-77 0.6580147659412852 1103 78-79 0.7788357844744596 1103 80-81 0.6227081196769291 1103 82-83 0.771082771195168 1103 84-85 1.0364175702373153 1103 86-87 1.092739410738396 1103 88-89 0.930492096318698 1103 90-91 0.8704560911053747 1103 92-93 1.138975766260824 1103 94-95 1.1081994361861156 1103 96-97 1.1671625300518862 1103 98-99 1.2921169194151823 1103 100-101 1.7626941899060178 1104 1 0.10969010124708944 1104 2 0.16115505968101473 1104 3 0.16672791945427434 1104 4 0.1436468320986677 1104 5 0.07577127107807513 1104 6 0.03420591285644292 1104 7 0.0473499465273548 1104 8 0.1332017324932977 1104 9 0.22713746557681702 1104 10-11 0.1650818982066653 1104 12-13 0.17154262639015627 1104 14-15 0.37536774300188114 1104 16-17 0.44638628343538045 1104 18-19 0.36973296072009276 1104 20-21 0.3575642086492863 1104 22-23 0.2880869816540894 1104 24-25 0.2449691559378877 1104 26-27 0.24913702599764775 1104 28-29 0.11145320002118098 1104 30-31 0.36143113298510343 1104 32-33 0.22530006798398006 1104 34-35 0.2635887523173679 1104 36-37 0.3256694352591367 1104 38-39 0.36185300614392446 1104 40-41 0.4165865542999043 1104 42-43 0.37275097915175337 1104 44-45 0.43885119772928505 1104 46-47 0.46858337877253575 1104 48-49 0.4651915350371567 1104 50-51 0.49170260690006273 1104 52-53 0.457593609099483 1104 54-55 0.5544403540589897 1104 56-57 0.5976231807892063 1104 58-59 0.5132176527069134 1104 60-61 0.6653354113085257 1104 62-63 0.7785973549665215 1104 64-65 0.5847917889972436 1104 66-67 0.5342991098006564 1104 68-69 0.6838443586856826 1104 70-71 0.7138121840552181 1104 72-73 0.7302178368504535 1104 74-75 0.9338629430882897 1104 76-77 0.8619785539830787 1104 78-79 0.8346786223165985 1104 80-81 0.8596497816863007 1104 82-83 1.0623568955173255 1104 84-85 1.1015238527915336 1104 86-87 1.260900279065595 1104 88-89 1.1661636074712938 1104 90-91 1.2160962246526665 1104 92-93 1.3612827001827625 1104 94-95 1.4449868273677566 1104 96-97 1.5426573470089586 1104 98-99 1.700942527455215 1104 100-101 2.3158286317204713 1105 1 0.2502563398581579 1105 2 0.1632045357333567 1105 3 0.2575280802501396 1105 4 0.19304308315351193 1105 5 0.24497498474658386 1105 6 0.2459304746112636 1105 7 0.29900964549750597 1105 8 0.16816038312065018 1105 9 0.20801909080834236 1105 10-11 0.3310060679656317 1105 12-13 0.31204854790670566 1105 14-15 0.3258187880817829 1105 16-17 0.47751512485120173 1105 18-19 0.4830287408354721 1105 20-21 0.3394368707792168 1105 22-23 0.3898165160732461 1105 24-25 0.31416265205839267 1105 26-27 0.38142938136994076 1105 28-29 0.3396412055147948 1105 30-31 0.43309259578744275 1105 32-33 0.3620281070612563 1105 34-35 0.34979521167222316 1105 36-37 0.32047400787289604 1105 38-39 0.3619707503330645 1105 40-41 0.47219308883205 1105 42-43 0.6381503435755178 1105 44-45 0.6177631202280693 1105 46-47 0.6260696811053137 1105 48-49 0.6302340217645934 1105 50-51 0.7333002585047979 1105 52-53 0.7357122825634335 1105 54-55 0.7051900200309333 1105 56-57 0.8982814295387698 1105 58-59 0.8456987157495561 1105 60-61 0.7995615028987757 1105 62-63 0.8512857466014125 1105 64-65 0.8633556622832401 1105 66-67 0.9354382731773825 1105 68-69 0.9119025472486584 1105 70-71 1.1281345946805779 1105 72-73 0.9979220694137751 1105 74-75 1.001133237506565 1105 76-77 1.187607147604723 1105 78-79 1.2254679502741581 1105 80-81 1.3563114122950921 1105 82-83 1.2609023119588514 1105 84-85 1.2017519390410634 1105 86-87 1.2780369529404823 1105 88-89 1.177637979138705 1105 90-91 1.3807038596221872 1105 92-93 1.4598792882675333 1105 94-95 1.569126259980603 1105 96-97 1.617882785645687 1105 98-99 1.8610887483981315 1105 100-101 2.159140482099769 1106 1 0.06529520433940306 1106 2 0.17678316590120602 1106 3 0.10958395498259321 1106 4 0.10927322847287968 1106 5 0.09775886443566151 1106 6 -0.07833307163183179 1106 7 -0.09654487742690776 1106 8 -0.16203281277886816 1106 9 0.02223419726322362 1106 10-11 -0.1001693009653124 1106 12-13 -0.1992517224548962 1106 14-15 -0.2176157900414566 1106 16-17 -0.34307741767442224 1106 18-19 -0.03142363609367038 1106 20-21 0.04644867093691829 1106 22-23 0.0025967346155510995 1106 24-25 -0.16589184270335267 1106 26-27 -0.18257098474473565 1106 28-29 -0.0028407703779649296 1106 30-31 0.048200458804231516 1106 32-33 0.10553168372003796 1106 34-35 0.08191191776662521 1106 36-37 0.019529150375994675 1106 38-39 -0.011107147605422085 1106 40-41 0.012920530535332375 1106 42-43 -0.015565016343373372 1106 44-45 0.06855378712003102 1106 46-47 0.07550853995402207 1106 48-49 0.24087387757173673 1106 50-51 0.1502119403793003 1106 52-53 0.1598659944030132 1106 54-55 0.15448384114167624 1106 56-57 0.14591646952975879 1106 58-59 0.20209516162091035 1106 60-61 0.1235729932671461 1106 62-63 0.39298268813571013 1106 64-65 0.29814289332976784 1106 66-67 0.3225432917647453 1106 68-69 0.3856739508760114 1106 70-71 0.40504050320823026 1106 72-73 0.3817566982298217 1106 74-75 0.6659918609153728 1106 76-77 0.783027897858183 1106 78-79 0.9663756330498714 1106 80-81 0.7649700244388313 1106 82-83 0.3984487803603365 1106 84-85 0.34856040473893657 1106 86-87 0.6848142988906929 1106 88-89 0.6531505396884363 1106 90-91 0.9595846752130406 1106 92-93 0.7025217913654274 1106 94-95 0.9434764064264733 1106 96-97 1.2174772432327075 1106 98-99 1.3359972197285366 1106 100-101 1.7144385839359764 1107 1 -0.020558882182836413 1107 2 -0.012729001589800504 1107 3 0.09138430014596821 1107 4 0.04850205829190912 1107 5 0.007747511645916916 1107 6 -0.07947883566735214 1107 7 -0.009553945196550728 1107 8 0.15151268033233123 1107 9 0.16335817165557387 1107 10-11 0.2204884979914823 1107 12-13 0.08191843441124291 1107 14-15 0.2742912496523289 1107 16-17 0.26909875062646194 1107 18-19 0.2536500365217691 1107 20-21 0.16287858011460088 1107 22-23 0.19319416613725338 1107 24-25 0.2472940851401475 1107 26-27 0.2716085170823135 1107 28-29 0.262989140245935 1107 30-31 0.25391795029566566 1107 32-33 0.1332055549490292 1107 34-35 0.2828442445341395 1107 36-37 0.1690850417748706 1107 38-39 0.26282229155229686 1107 40-41 0.10967100537600771 1107 42-43 0.19976890270714165 1107 44-45 0.27442560183093434 1107 46-47 0.07539832629981902 1107 48-49 0.28384045071648245 1107 50-51 0.19890624904020626 1107 52-53 0.20824442554870615 1107 54-55 0.22585414214727706 1107 56-57 0.26043098712055723 1107 58-59 0.243553392513995 1107 60-61 0.26786309440579714 1107 62-63 0.3203103113291448 1107 64-65 0.31488289516163803 1107 66-67 0.14048461640150833 1107 68-69 0.2763588181616683 1107 70-71 0.5089555886795516 1107 72-73 0.38681266676017856 1107 74-75 0.42902925040042206 1107 76-77 0.5376596432471672 1107 78-79 0.6457013833945524 1107 80-81 0.7152948202814429 1107 82-83 0.5485177166208111 1107 84-85 0.8084047317185643 1107 86-87 0.9454778465531248 1107 88-89 0.9236265373359274 1107 90-91 1.0580796472046679 1107 92-93 0.9682909609486288 1107 94-95 1.2200675445014646 1107 96-97 1.2010426941144168 1107 98-99 1.4889806412809996 1107 100-101 1.7662126682406836 1108 1 0.0969391364930523 1108 2 0.007151238149656791 1108 3 0.02763882673291107 1108 4 0.0836076044080869 1108 5 0.046931661215182885 1108 6 0.1010969002758344 1108 7 0.05377767752690232 1108 8 0.027055827687604506 1108 9 0.10211475659981772 1108 10-11 0.09590965923239736 1108 12-13 0.11484596837502892 1108 14-15 0.10067348612253824 1108 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1.2387869117464128 2311 92-93 1.634049797955214 2311 94-95 1.7664286429598484 2311 96-97 1.8302811471815836 2311 98-99 1.9194222858760064 2311 100-101 2.2305127315072397 2312 1 0.23728182481485405 2312 2 0.2364499719379225 2312 3 0.24431297979046462 2312 4 0.2741728820387621 2312 5 0.35894978149057977 2312 6 0.14122004826622714 2312 7 0.23204141254243638 2312 8 0.21412196761375668 2312 9 0.31874048882536243 2312 10-11 0.2860208694643518 2312 12-13 0.2999643210285541 2312 14-15 0.38093849459199447 2312 16-17 0.46871727033577315 2312 18-19 0.4379604976219369 2312 20-21 0.45158570155850697 2312 22-23 0.4812342928523208 2312 24-25 0.49670795620557584 2312 26-27 0.6496894480988047 2312 28-29 0.36749680378680694 2312 30-31 0.5074123211862656 2312 32-33 0.4397567364815558 2312 34-35 0.5228986577021146 2312 36-37 0.4908990583917685 2312 38-39 0.4616883168950281 2312 40-41 0.4293597253746597 2312 42-43 0.43538792635562373 2312 44-45 0.35535668800802256 2312 46-47 0.5685691559696266 2312 48-49 0.7477488029092143 2312 50-51 0.8567454091006823 2312 52-53 0.4936507871398419 2312 54-55 0.4771630809776113 2312 56-57 0.638074664488542 2312 58-59 0.7118428520601228 2312 60-61 0.7685725950670133 2312 62-63 0.7382702384084752 2312 64-65 0.9372359427464048 2312 66-67 1.0055617981160339 2312 68-69 0.842782072167779 2312 70-71 0.7654526901233716 2312 72-73 0.7966135452811471 2312 74-75 0.838982941232338 2312 76-77 1.0679022657154675 2312 78-79 0.9900451468475069 2312 80-81 0.9243162793665576 2312 82-83 0.929767221008607 2312 84-85 0.9341896107278664 2312 86-87 0.8030533583419697 2312 88-89 0.5361792499182023 2312 90-91 0.848032889285431 2312 92-93 1.024493977085971 2312 94-95 1.3326391408668066 2312 96-97 1.535399235826187 2312 98-99 1.6461558029324515 2312 100-101 1.9909863380872146 2313 1 0.16851371588723651 2313 2 0.22592546758924925 2313 3 0.17835520138985572 2313 4 0.1485581090553083 2313 5 0.1484531569029457 2313 6 0.19517603240808512 2313 7 0.14579383225051146 2313 8 0.267751914610578 2313 9 0.3300501395459463 2313 10-11 0.23258873801865576 2313 12-13 0.3333938838245487 2313 14-15 -0.06685538750867437 2313 16-17 0.0017264223629283038 2313 18-19 0.2250333568010987 2313 20-21 0.21982126908985578 2313 22-23 0.2976890880973997 2313 24-25 0.40374740606745974 2313 26-27 0.27882556987220397 2313 28-29 0.18929441148398496 2313 30-31 -0.583240029370728 2313 32-33 -0.0584786349213573 2313 34-35 0.19541332568305592 2313 36-37 0.3063350402813043 2313 38-39 0.4232906945620343 2313 40-41 0.36871323125460975 2313 42-43 0.3168915607418725 2313 44-45 0.38935164898374097 2313 46-47 0.36454803821054327 2313 48-49 0.35787027388926873 2313 50-51 0.5014611926516608 2313 52-53 0.5703208681282277 2313 54-55 0.669459093180599 2313 56-57 0.5200362482023806 2313 58-59 0.7277786029188107 2313 60-61 0.7044132932335856 2313 62-63 0.8784458598139722 2313 64-65 0.8826779708745889 2313 66-67 0.9872449849119107 2313 68-69 1.0068157674529559 2313 70-71 0.9364546385432853 2313 72-73 1.0280866321379918 2313 74-75 1.0555619722356049 2313 76-77 1.0569858208531784 2313 78-79 1.0343241966833148 2313 80-81 1.116861775361638 2313 82-83 1.440255873924773 2313 84-85 1.6581930516218257 2313 86-87 1.3693168229273986 2313 88-89 1.4523072488114472 2313 90-91 1.554990344812257 2313 92-93 1.622809465896001 2313 94-95 1.6845820038061454 2313 96-97 1.9455680939643862 2313 98-99 1.85591905483453 2313 100-101 2.0010906425578234 2314 1 0.09010260033323902 2314 2 0.03540540524378599 2314 3 0.052085239009301176 2314 4 0.04321005435885894 2314 5 0.016743199309829038 2314 6 0.022751930936216525 2314 7 0.19039015082168476 2314 8 0.01789784977891884 2314 9 0.06311118750462441 2314 10-11 0.11805424808450482 2314 12-13 0.039776269327326474 2314 14-15 0.033401801124433916 2314 16-17 -0.15858846030410945 2314 18-19 0.1704927737676556 2314 20-21 0.07794263601139306 2314 22-23 -0.04890557663253503 2314 24-25 0.1857292410151885 2314 26-27 0.08166566137750664 2314 28-29 0.1453166512964046 2314 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0.046385957185350435 2315 52-53 0.23822996167333343 2315 54-55 0.20614050540174134 2315 56-57 0.2222181611648395 2315 58-59 0.21322779002272796 2315 60-61 0.18428183549485055 2315 62-63 0.22369671958187354 2315 64-65 0.35258920121336956 2315 66-67 0.4404053588284995 2315 68-69 0.44182868986084145 2315 70-71 0.6206506500104645 2315 72-73 0.830231519239387 2315 74-75 0.9552158346032797 2315 76-77 0.9256897914466151 2315 78-79 0.9417037821784859 2315 80-81 0.8562809664198596 2315 82-83 1.0319144763673975 2315 84-85 1.2046880695404916 2315 86-87 0.9186642978879433 2315 88-89 1.0942290949297977 2315 90-91 1.3297709857037852 2315 92-93 1.292073412808179 2315 94-95 1.4512247619205283 2315 96-97 1.560110558516584 2315 98-99 1.9573675243632316 2315 100-101 2.023179659482974 2316 1 -0.2580430165794638 2316 2 -0.6218116532581277 2316 3 -0.4112371593869071 2316 4 -0.3970806327196925 2316 5 -0.2977934327295344 2316 6 -0.1001317031078841 2316 7 -0.06858788817959294 2316 8 -0.4518055400515948 2316 9 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-0.03523931106352052 2316 72-73 0.06066065717410041 2316 74-75 0.0829151258225096 2316 76-77 0.19513078153364916 2316 78-79 -0.07892006587486122 2316 80-81 0.044607121213026346 2316 82-83 0.02815308143560813 2316 84-85 -0.09758451217210862 2316 86-87 -0.16211874118750913 2316 88-89 -0.27546016412387786 2316 90-91 -0.31160911755333487 2316 92-93 -0.25581154196790834 2316 94-95 -0.2765749132216193 2316 96-97 0.17960456483583798 2316 98-99 0.1592010320452495 2316 100-101 0.2880028556857326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13011.0 3 2946.0 4 2897.0 5 3128.0 6 3553.0 7 4246.0 8 5129.0 9 6337.0 10 8034.0 11 9972.0 12 12491.0 13 15534.0 14 17310.0 15 20737.0 16 24876.0 17 31340.0 18 28262.0 19 21212.0 20 21246.0 21 22109.0 22 24535.0 23 26754.0 24 29458.0 25 33687.0 26 38377.0 27 44353.0 28 52121.0 29 61945.0 30 74777.0 31 90917.0 32 112630.0 33 144101.0 34 188941.0 35 258432.0 36 361865.0 37 476804.0 38 351761.0 39 42211.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18705465000071 19.154481544867693 15.045972696271207 22.61249110886039 2 15.010497621198368 19.955666346159944 41.38182051548973 23.652015517151963 3 18.873515611563747 24.64210714420285 30.02412626689016 26.460250977343247 4 12.437828760618627 16.746304042445416 37.582462917331554 33.2334042796044 5 13.40930987395389 37.57754094269943 34.431591460360174 14.58155772298651 6 31.454492716015963 36.373055410773155 17.648142224290186 14.524309648920694 7 28.149072129667356 33.1036040788344 20.75805807638066 17.98926571511758 8 26.01057384353247 35.45205418162701 19.72293994608682 18.814432028753696 9 26.12312284656567 17.006860043407045 20.81811955244732 36.05189755757996 10-11 24.550696927315457 27.17127264226113 27.219520452559987 21.058509977863427 12-13 25.4332150254658 24.462735072339058 28.08687435137753 22.01717555081761 14-15 22.619062487708387 25.73609391191722 26.47691805524202 25.167925545132373 16-17 21.556893036583798 29.130713346067715 27.16379353894386 22.14860007840463 18-19 21.480079530511986 27.451821070515287 29.88361224811641 21.184487150856317 20-21 22.31507225409554 26.46735989195482 30.093512536338597 21.12405531761104 22-23 22.04346907183255 26.334389575593388 30.05316125319212 21.56898009938195 24-25 21.738919748645603 26.620056464532983 30.055230722550704 21.585793064270714 26-27 21.420526944839818 26.750238405371896 30.088510091313548 21.740724558474742 28-29 21.538391270021037 26.896344083616004 30.127394531552216 21.437870114810746 30-31 21.642672898391407 26.70976454124513 30.041632032337763 21.605930528025706 32-33 21.458606255654583 26.782793696731794 29.957321116604717 21.801278931008902 34-35 21.742928357202334 26.770303751205383 29.893303910214 21.59346398137828 36-37 21.840620579239598 26.514833252878212 29.829385440915807 21.815160726966383 38-39 21.51081052821298 26.856217542461447 29.87460670731196 21.758365222013616 40-41 21.840560727180016 26.898090131139014 29.72215243614698 21.53919670553399 42-43 21.618599962608606 26.762563349612694 29.65266080023173 21.96617588754697 44-45 21.7173299613928 26.99771726733396 29.552469207712644 21.7324835635606 46-47 21.90661836151289 27.160465523305522 28.97250524178765 21.960410873393936 48-49 21.922279455666512 27.27136649492905 28.84343766770887 21.962916381695564 50-51 22.133300621585352 27.467279241375785 28.594026203452405 21.80539393358645 52-53 22.007272724560707 27.488292248449515 28.547970317677812 21.956464709311962 54-55 22.00635823658195 27.44234294773515 28.553086428958217 21.99821238672469 56-57 21.991204286047054 27.789033310039603 28.370333100395996 21.849429303517354 58-59 21.974906213702123 27.4298203063664 28.57126351680867 22.024009963122804 60-61 22.08331407562242 27.410649191101594 28.623735886582768 21.882300846693216 62-63 21.87353925041134 27.48322770583942 28.823576116282933 21.819656927466305 64-65 21.978093039265143 27.358635959327305 28.720119819823008 21.94315118158454 66-67 21.876429788008856 27.49772025980372 28.58407493612721 22.041775016060217 68-69 21.833310750855883 27.48359570941626 28.62631876853619 22.056774771191666 70-71 21.947962226927302 27.39500092529902 28.566897255281702 22.090139592491976 72-73 21.91339744609984 27.37061743792205 28.455143489619537 22.26084162635857 74-75 21.828939231795424 27.775187201734308 28.215585129124378 22.180288437345894 76-77 22.021673570021377 27.593548518178647 28.233015203038036 22.15176270876194 78-79 21.98800823560005 27.551307061309366 28.21295121739803 22.247733485692557 80-81 21.813901281768352 27.843793056143724 28.163713251301747 22.17859241078618 82-83 22.132356011105774 27.798810691844533 27.972990805824864 22.095842491224825 84-85 21.973391283441444 27.758406198077175 28.013515893568865 22.25468662491252 86-87 22.03354419209027 27.64588104151714 28.11509939672192 22.20547536967067 88-89 22.122842614237676 27.514112310243803 28.067123265713285 22.295921809805236 90-91 22.027227192673607 27.608654444208558 28.13311312207243 22.231005241045406 92-93 21.93791862655543 27.475955270456726 28.19050817724893 22.395617925738907 94-95 21.883128598576487 27.587266062241255 28.108316453308625 22.421288885873636 96-97 21.97958535596293 27.469552467111146 28.036124810658013 22.514737366267905 98-99 21.85890232931239 27.52272905600962 28.004977738282566 22.61339087639542 100-101 22.300027208450274 27.04191964934644 28.304074931326618 22.353978210876672 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3811.0 1 2244.0 2 656.5 3 620.5 4 550.5 5 543.0 6 864.0 7 1763.5 8 2996.5 9 3755.5 10 3596.5 11 3331.5 12 3521.5 13 3740.5 14 3948.0 15 4048.0 16 4151.0 17 4441.0 18 4683.5 19 5011.5 20 5602.5 21 6500.5 22 7677.5 23 9267.5 24 11505.5 25 14476.5 26 18330.0 27 22321.0 28 25201.5 29 28371.0 30 32902.0 31 38172.0 32 44168.0 33 49401.0 34 54898.5 35 60410.5 36 67352.0 37 74776.5 38 77613.5 39 79369.0 40 82404.5 41 87250.5 42 91022.0 43 93658.0 44 98575.5 45 101653.5 46 102845.5 47 104819.5 48 106272.5 49 104857.0 50 100108.5 51 93218.5 52 87923.5 53 86279.5 54 84556.0 55 77639.5 56 68330.0 57 60913.5 58 54471.0 59 47862.5 60 39626.0 61 31693.0 62 25073.0 63 18668.5 64 13675.0 65 10499.5 66 7843.0 67 5846.0 68 4428.0 69 3251.0 70 2340.5 71 1581.5 72 1123.5 73 844.0 74 639.0 75 477.0 76 341.5 77 238.0 78 147.5 79 100.5 80 70.5 81 47.5 82 34.5 83 19.0 84 16.0 85 15.5 86 12.0 87 10.5 88 7.0 89 7.0 90 6.0 91 3.0 92 2.5 93 2.5 94 1.5 95 3.5 96 3.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3130523290312044 2 0.29456308274142323 3 0.2815424867627041 4 0.2658805698854448 5 0.2621231979030144 6 0.21811358349494372 7 0.20892476290424764 8 0.22168494696339242 9 0.223061409966857 10-11 0.22551672235141548 12-13 0.22032708481132598 14-15 0.21701613326245167 16-17 0.2161232923953395 18-19 0.21606748984114502 20-21 0.20340031003899112 22-23 0.1959785703311212 24-25 0.19728062992899312 26-27 0.20992920887974884 28-29 0.2241960619021454 30-31 0.21798337753515654 32-33 0.22482849084968318 34-35 0.21593728388135783 36-37 0.20066598488346007 38-39 0.20124261127680335 40-41 0.19519733457239805 42-43 0.2088317586472568 44-45 0.20529759688160445 46-47 0.20533479858440082 48-49 0.2142632072555225 50-51 0.22454947807871062 52-53 0.2375514732060316 54-55 0.21210550849333473 56-57 0.1831811845691801 58-59 0.1843158365044685 60-61 0.19125395407598597 62-63 0.19906631166321745 64-65 0.18677114888902693 66-67 0.1926118162280524 68-69 0.19681560864403888 70-71 0.19093773960221708 72-73 0.19240720686267251 74-75 0.19523453627519438 76-77 0.21046863357029577 78-79 0.2154536617450054 80-81 0.20479537389385388 82-83 0.2039025330267417 84-85 0.1964807933188718 86-87 0.19677840694124252 88-89 0.2031957006736112 90-91 0.20362352025576916 92-93 0.20061018232926556 94-95 0.20055437977507107 96-97 0.18872423828583482 98-99 0.19733643248318763 100-101 0.18820341444668603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2688049.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.21615626945935 #Duplication Level Percentage of deduplicated Percentage of total 1 73.9571041493865 37.87798603351659 2 13.817939267145253 14.154034736560195 3 4.961724985538061 7.623614466761946 4 2.3504819156082277 4.815305963933167 5 1.311328862683198 3.358061197591753 6 0.7925420526553952 2.435457457135008 7 0.5196325691731838 1.8629508005832094 8 0.3840203624508139 1.573443751514824 9 0.2697417237638027 1.2433620849012228 >10 1.460978424079329 13.719225422882927 >50 0.1127756487842118 3.9667326672290435 >100 0.058967981381558815 5.203186579107276 >500 0.0022532573122129905 0.7668355084964574 >1k 2.9074287899244906E-4 0.34594894802681997 >5k 7.268571974811227E-5 0.23773054794999166 >10k+ 1.4537143949622453E-4 0.8161238338095314 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11807 0.439240504916391 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10116 0.37633242548777945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6386 0.23757007405742978 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3886 0.14456581706657878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.7201702796340396E-5 7 0.0 0.0 0.0 0.0 3.7201702796340396E-5 8 0.0 0.0 0.0 0.0 3.7201702796340396E-5 9 0.0 0.0 0.0 7.440340559268079E-5 3.7201702796340396E-5 10-11 0.0 0.0 0.0 7.440340559268079E-5 3.7201702796340396E-5 12-13 0.0 0.0 0.0 7.440340559268079E-5 7.440340559268079E-5 14-15 0.0 0.0 0.0 7.440340559268079E-5 7.440340559268079E-5 16-17 0.0 0.0 0.0 9.300425699085098E-5 7.440340559268079E-5 18-19 0.0 0.0 0.0 1.1160510838902119E-4 9.300425699085098E-5 20-21 0.0 0.0 0.0 1.4880681118536158E-4 1.1160510838902119E-4 22-23 0.0 0.0 0.0 2.2321021677804237E-4 1.4880681118536158E-4 24-25 0.0 0.0 0.0 4.092187307597443E-4 1.4880681118536158E-4 26-27 0.0 0.0 0.0 6.324289475377867E-4 1.4880681118536158E-4 28-29 0.0 0.0 0.0 0.0013578621520664244 1.4880681118536158E-4 30-31 0.0 0.0 0.0 0.002157698762187743 1.4880681118536158E-4 32-33 0.0 0.0 0.0 0.003496960062855997 1.4880681118536158E-4 34-35 0.0 0.0 0.0 0.005226839242885826 1.8600851398170197E-4 36-37 0.0 0.0 0.0 0.007179928639693696 1.8600851398170197E-4 38-39 0.0 0.0 0.0 0.009188820590696076 1.8600851398170197E-4 40-41 0.0 0.0 0.0 0.0114209227584765 1.8600851398170197E-4 42-43 0.0 0.0 0.0 0.015680517728657476 1.8600851398170197E-4 44-45 0.0 0.0 0.0 0.023232463396314577 1.8600851398170197E-4 46-47 0.0 0.0 0.0 0.03751791727010929 1.8600851398170197E-4 48-49 0.0 0.0 0.0 0.06287087772581526 1.8600851398170197E-4 50-51 0.0 0.0 0.0 0.08424325598231283 1.8600851398170197E-4 52-53 0.0 0.0 0.0 0.10299291419166838 2.2321021677804237E-4 54-55 0.0 0.0 0.0 0.12998274957041334 2.2321021677804237E-4 56-57 0.0 0.0 0.0 0.15102031250174383 2.2321021677804237E-4 58-59 0.0 0.0 0.0 0.17962842195212958 2.2321021677804237E-4 60-61 0.0 0.0 0.0 0.2236194355088021 2.2321021677804237E-4 62-63 0.0 0.0 0.0 0.25817981740660234 2.2321021677804237E-4 64-65 0.0 0.0 0.0 0.29925049729376213 2.2321021677804237E-4 66-67 0.0 0.0 0.0 0.36076351286751096 2.2321021677804237E-4 68-69 0.0 0.0 0.0 0.432674404372837 2.2321021677804237E-4 70-71 0.0 0.0 0.0 0.5014045502890758 2.2321021677804237E-4 72-73 0.0 0.0 0.0 0.57485931246045 2.604119195743828E-4 74-75 0.0 0.0 0.0 0.6434406515655035 2.9761362237072317E-4 76-77 0.0 0.0 0.0 0.7099758970167582 2.9761362237072317E-4 78-79 0.0 0.0 0.0 0.789401532486945 2.9761362237072317E-4 80-81 0.0 0.0 0.0 0.8908691768639634 3.3481532516706355E-4 82-83 0.0 0.0 0.0 1.0116259041408844 3.3481532516706355E-4 84-85 0.0 0.0 0.0 1.14341293629692 3.3481532516706355E-4 86-87 0.0 0.0 0.0 1.3340902639795629 3.3481532516706355E-4 88-89 0.0 0.0 0.0 1.5845321272045263 3.3481532516706355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6715 0.0 46.94319 1 GTATCAA 11505 0.0 41.32553 1 ATCAACG 14605 0.0 32.247112 3 TATCAAC 14640 0.0 32.111683 2 TCAACGC 14750 0.0 31.86745 4 CAACGCA 15015 0.0 31.336672 5 AACGCAG 15570 0.0 30.48131 6 CGCAGAG 17705 0.0 26.429993 8 ACGCAGA 17950 0.0 26.201582 7 GCAGAGT 18810 0.0 24.194086 9 GAGTACT 10850 0.0 23.574797 12-13 GTACATG 11075 0.0 23.096363 1 TATAACG 145 2.3228167E-5 22.947634 2 TACATGG 11030 0.0 22.538969 2 TGGTATC 3390 0.0 22.154667 2 GTACTTT 11800 0.0 21.434702 14-15 AGTACTT 11355 0.0 21.396883 12-13 ACATGGG 11245 0.0 20.877872 3 GTGGTAT 3860 0.0 20.569998 1 CAGAGTA 18505 0.0 20.496984 10-11 >>END_MODULE