FastQCFastQC Report
Thu 26 May 2016
SRR937877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688049
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136440.5075800329532684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123200.4583249784509137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81260.30230103692306204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.11640412804974909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.051.360161
GTATCAA120650.038.6773221
ATCAACG156200.029.6185863
TCAACGC159000.029.1268734
TATCAAC160050.028.9951442
CAACGCA161350.028.732095
AACGCAG169450.027.385666
CGCAGAG191800.023.9499538
ACGCAGA194400.023.7762497
GGACCGA6450.022.8282286
GTACATG112800.022.4959551
TACATGG113600.022.1607232
GCAGAGT205600.021.9958429
GAGTACT124550.021.9687612-13
AGTACTT128450.020.95041312-13
GTACTTT130250.020.82229614-15
ACATGGG113050.020.7979743
AGAGTAC194850.019.89374510-11
CAGAGTA202150.019.84507210-11
AGGACCG7800.019.4868555
Produced by FastQC (version 0.11.5)