FastQCFastQC Report
Thu 26 May 2016
SRR937876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213151
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100010.4518896360889971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83000.3750308948643811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50890.22994364144154647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31810.1437317200679032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.051.59831
GTATCAA95850.042.016141
ATCAACG122500.032.6695673
TATCAAC124200.032.298942
TCAACGC125050.032.0830274
CAACGCA127550.031.4527725
AACGCAG132700.030.2865946
CGCAGAG149300.026.6327868
ACGCAGA151300.026.406337
GCAGAGT156450.024.7779629
GTACATG92400.024.5454651
TACATGG92900.024.0463712
GAGTACT91500.023.72287812-13
ACATGGG94500.022.5830153
GTACTTT98150.022.04148514-15
CAGAGTA154550.021.11420610-11
AGTACTT96650.021.10734412-13
AGAGTAC150200.020.4449310-11
TGGTATC27200.020.0953852
GTATAGG6400.020.0589711
Produced by FastQC (version 0.11.5)