##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937876_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2213151 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.582888379509576 33.0 31.0 34.0 30.0 34.0 2 31.6842669117471 33.0 31.0 34.0 30.0 34.0 3 31.719157436614132 33.0 31.0 34.0 30.0 34.0 4 35.336209323268044 37.0 35.0 37.0 33.0 37.0 5 35.33027750930687 37.0 35.0 37.0 33.0 37.0 6 35.38213388964422 37.0 35.0 37.0 33.0 37.0 7 35.29548277546358 37.0 35.0 37.0 33.0 37.0 8 35.29781067807845 37.0 35.0 37.0 33.0 37.0 9 36.83851982987153 39.0 37.0 39.0 33.0 39.0 10-11 36.74447247386193 39.0 37.0 39.0 33.0 39.0 12-13 36.63385485219942 39.0 37.0 39.0 32.0 39.0 14-15 37.715236782307215 40.0 37.5 41.0 32.0 41.0 16-17 37.62084783189218 40.0 37.0 41.0 32.0 41.0 18-19 37.550059620875395 40.0 37.0 41.0 32.0 41.0 20-21 37.56702005421229 40.0 37.0 41.0 32.0 41.0 22-23 37.56438421960363 40.0 37.0 41.0 32.0 41.0 24-25 37.483189579021044 40.0 37.0 41.0 32.0 41.0 26-27 37.3680395056641 40.0 37.0 41.0 32.0 41.0 28-29 37.21413699291192 40.0 37.0 41.0 31.0 41.0 30-31 36.96003051757427 39.0 36.5 41.0 31.0 41.0 32-33 36.8107485661846 39.0 36.0 41.0 30.0 41.0 34-35 36.78320706540132 39.0 36.0 41.0 30.0 41.0 36-37 36.60563942541653 39.0 36.0 41.0 30.0 41.0 38-39 36.42590699866389 39.0 35.5 41.0 30.0 41.0 40-41 36.185928117873566 39.0 35.0 41.0 29.5 41.0 42-43 35.90759216158319 39.0 35.0 40.5 28.5 41.0 44-45 35.62613599343199 39.0 35.0 40.0 27.5 41.0 46-47 35.23117672495008 38.5 35.0 40.0 25.5 41.0 48-49 34.84399708831435 38.0 34.5 40.0 24.0 41.0 50-51 33.963134463034834 37.5 33.5 39.5 23.5 40.5 52-53 34.060296156927386 38.0 33.5 39.5 23.0 40.5 54-55 34.312746848271985 38.0 34.0 40.0 23.0 41.0 56-57 34.05045905137065 38.0 34.0 40.0 21.5 41.0 58-59 33.75134457612698 38.0 33.0 40.0 20.5 41.0 60-61 33.381511925756534 37.5 33.0 40.0 18.5 41.0 62-63 32.994529293301724 37.0 32.5 40.0 17.0 41.0 64-65 32.533976669463584 36.5 31.5 39.0 12.5 41.0 66-67 32.001287756687184 36.0 31.0 39.0 8.0 40.0 68-69 31.51527234246556 35.0 31.0 39.0 7.5 40.0 70-71 31.024578530791615 35.0 30.0 38.0 4.0 40.0 72-73 30.527855532677165 35.0 29.5 37.5 2.0 39.5 74-75 29.95745794118883 34.0 29.0 37.0 2.0 39.0 76-77 29.38849360030111 34.0 29.0 36.0 2.0 39.0 78-79 28.837605070779176 34.0 28.0 36.0 2.0 38.0 80-81 28.266700509816097 34.0 26.5 35.0 2.0 37.0 82-83 27.699466507255945 33.0 26.0 35.0 2.0 37.0 84-85 27.117446120937977 33.0 25.5 35.0 2.0 36.0 86-87 26.507714792167366 32.5 24.5 35.0 2.0 36.0 88-89 25.920331012208386 32.0 23.5 35.0 2.0 35.0 90-91 25.304434491817325 32.0 20.0 34.0 2.0 35.0 92-93 24.59084039001406 31.0 17.0 34.0 2.0 35.0 94-95 23.776528352561574 31.0 4.5 34.0 2.0 35.0 96-97 22.842524075401997 30.5 2.0 34.0 2.0 35.0 98-99 21.61710407468808 30.0 2.0 34.0 2.0 35.0 100-101 18.522874851286694 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11313902751092542 1101 2 -0.02654467658208759 1101 3 0.09804529031973885 1101 4 0.14541758345217914 1101 5 0.08121762472647731 1101 6 0.16214767086373172 1101 7 0.04150175707299297 1101 8 0.03235000981321434 1101 9 0.17393266537226992 1101 10-11 0.23978419026956743 1101 12-13 0.2418786907279582 1101 14-15 0.3074048683274597 1101 16-17 0.2989124551096438 1101 18-19 0.3994862128320378 1101 20-21 0.24294649047096328 1101 22-23 0.1378974481954316 1101 24-25 0.15304114497045873 1101 26-27 0.24349967489967383 1101 28-29 0.17323595938194813 1101 30-31 0.12718323403505138 1101 32-33 0.1298318404184684 1101 34-35 0.20866909472222517 1101 36-37 0.3857640613248279 1101 38-39 0.20074607310677806 1101 40-41 0.209363755048102 1101 42-43 0.04908922193200027 1101 44-45 0.21246659593499828 1101 46-47 0.14633790929668322 1101 48-49 0.260581234566736 1101 50-51 0.12606383732761373 1101 52-53 0.2986850433608552 1101 54-55 0.44521630204216933 1101 56-57 0.560359980672068 1101 58-59 0.5994512842543216 1101 60-61 0.46725160054607073 1101 62-63 0.4851779044497917 1101 64-65 0.5827181817133251 1101 66-67 0.5566180036284578 1101 68-69 0.7746037040214624 1101 70-71 0.7814957895291812 1101 72-73 0.6936727077473108 1101 74-75 0.7514385375972772 1101 76-77 0.5895006273848722 1101 78-79 0.8666561639886865 1101 80-81 0.9805329070646103 1101 82-83 1.030029151346323 1101 84-85 1.2204218542194667 1101 86-87 1.4826470239675125 1101 88-89 1.5127871477126504 1101 90-91 1.604733757959199 1101 92-93 1.4382197843086288 1101 94-95 1.7610304026183385 1101 96-97 1.7839034819999071 1101 98-99 1.9413802852998963 1101 100-101 2.668970924450143 1102 1 -0.13159561759146854 1102 2 -0.13007410399921682 1102 3 -0.20379615138648433 1102 4 -0.09376966521638508 1102 5 -0.11476669645525561 1102 6 -0.13062701612383876 1102 7 -0.09464863524519274 1102 8 -0.12415635808143577 1102 9 0.035937172594074696 1102 10-11 0.05658600404382952 1102 12-13 2.769669408806408E-4 1102 14-15 0.171614455048541 1102 16-17 0.22407279772622957 1102 18-19 0.029418689170334744 1102 20-21 0.17333219871347438 1102 22-23 0.002852241419901702 1102 24-25 0.09766832551967752 1102 26-27 0.09932678457187194 1102 28-29 0.05360877127332486 1102 30-31 0.1978881972706219 1102 32-33 0.1840662129285917 1102 34-35 0.1494157284419586 1102 36-37 0.10897298795835297 1102 38-39 0.26181082488452745 1102 40-41 0.2563624127995041 1102 42-43 0.17777454703344375 1102 44-45 0.27199890439394636 1102 46-47 0.25946309884886176 1102 48-49 0.3340990537559634 1102 50-51 0.09215021354970787 1102 52-53 0.14228753095056845 1102 54-55 0.13263294066343434 1102 56-57 0.2734882847005977 1102 58-59 0.1524067560113096 1102 60-61 0.2426143992447649 1102 62-63 0.3841901014044353 1102 64-65 0.40844024886687436 1102 66-67 0.5285279970759618 1102 68-69 0.4032512320797217 1102 70-71 0.4129498236378346 1102 72-73 0.5607311332071561 1102 74-75 0.48804545772184227 1102 76-77 0.48805411942345245 1102 78-79 0.7594992787432275 1102 80-81 0.9293535939608581 1102 82-83 1.045572656196594 1102 84-85 1.1793075913389153 1102 86-87 1.286625208118938 1102 88-89 1.2127703960631102 1102 90-91 1.2985976650183844 1102 92-93 1.5328424140938495 1102 94-95 1.51778269319475 1102 96-97 1.6180697109768154 1102 98-99 1.7805076029767868 1102 100-101 2.5848223088953617 1103 1 -0.08591272192498067 1103 2 -0.13968605626072517 1103 3 -0.020466527687382552 1103 4 0.03808233417647955 1103 5 -0.01646408909234509 1103 6 -0.050887578432394776 1103 7 0.04735516318027777 1103 8 -0.010027911146302415 1103 9 -0.13486018678629108 1103 10-11 0.04003448602350801 1103 12-13 -0.025700621668882206 1103 14-15 0.04912013298869766 1103 16-17 0.08863640175487575 1103 18-19 0.16023924026399783 1103 20-21 0.037327808746319135 1103 22-23 -0.017418709864301718 1103 24-25 -0.034881005116325525 1103 26-27 -0.06389022223125806 1103 28-29 -0.06597103815746408 1103 30-31 -0.040508896775911296 1103 32-33 -0.06007711453931819 1103 34-35 -0.11890206542356907 1103 36-37 0.0634753740230849 1103 38-39 0.014215237809409587 1103 40-41 -0.005863381737150064 1103 42-43 -0.07511686787304228 1103 44-45 -0.052912609330796556 1103 46-47 -0.0768434413262824 1103 48-49 0.119408272267016 1103 50-51 0.005923815472556271 1103 52-53 0.03320883610988545 1103 54-55 0.0384602260930933 1103 56-57 -0.007329076707570437 1103 58-59 0.08958944979770678 1103 60-61 0.1887400112594193 1103 62-63 0.32916067730565146 1103 64-65 0.20448405894720167 1103 66-67 0.4907857684597019 1103 68-69 0.5557946412995669 1103 70-71 0.580161736543225 1103 72-73 0.5288586258363814 1103 74-75 0.6527583670289836 1103 76-77 0.6647706451740163 1103 78-79 0.7448002280696286 1103 80-81 0.7791094080375913 1103 82-83 0.9526772156870251 1103 84-85 1.1158890944027107 1103 86-87 1.2117790379513202 1103 88-89 1.3853237822992277 1103 90-91 1.410677763920635 1103 92-93 1.3368830356885582 1103 94-95 1.5692738661650942 1103 96-97 1.5881141645936871 1103 98-99 1.8778932007568443 1103 100-101 2.4616810006283565 1104 1 0.08618080140026052 1104 2 0.13854282265588225 1104 3 0.07234756556471211 1104 4 0.1676795645268001 1104 5 0.04989013268081521 1104 6 0.17552949070255863 1104 7 0.19426596203655322 1104 8 0.15317710067830603 1104 9 0.22369537460656375 1104 10-11 0.17733349114390506 1104 12-13 0.22950367094603052 1104 14-15 0.29193109038875775 1104 16-17 0.3627808333979843 1104 18-19 0.40885376382015437 1104 20-21 0.3126587734932329 1104 22-23 0.18822975379218576 1104 24-25 0.23970061317066182 1104 26-27 0.16334776816814411 1104 28-29 0.10685981764913066 1104 30-31 0.24451531240904245 1104 32-33 0.1969432232937578 1104 34-35 0.24511110026793403 1104 36-37 0.09389996024333414 1104 38-39 0.24213802498727688 1104 40-41 0.2700394687523442 1104 42-43 0.2900130887585277 1104 44-45 0.3575543353271158 1104 46-47 0.2807637284532305 1104 48-49 0.46157177803555527 1104 50-51 0.26202264042400003 1104 52-53 0.18014203390924166 1104 54-55 0.4562112052323428 1104 56-57 0.5581448666019284 1104 58-59 0.6424727435663584 1104 60-61 0.530197848507072 1104 62-63 0.6421919140571859 1104 64-65 0.6900412204390989 1104 66-67 0.7507561489118615 1104 68-69 0.7114305411852548 1104 70-71 0.8599689264410557 1104 72-73 0.673076257225528 1104 74-75 0.6715347901458344 1104 76-77 0.7103897580699865 1104 78-79 0.9666133980780103 1104 80-81 1.1517695838977993 1104 82-83 1.1026157407695685 1104 84-85 1.2466665454274235 1104 86-87 1.3338101089506758 1104 88-89 1.4971069295787949 1104 90-91 1.459743157838627 1104 92-93 1.479683628486768 1104 94-95 1.518631281213029 1104 96-97 1.5575243352614159 1104 98-99 1.9361430776644717 1104 100-101 2.424226913371033 1105 1 0.09967151199717961 1105 2 0.12910915634623166 1105 3 0.09494409158246597 1105 4 -0.014020744413009822 1105 5 -0.018748897304725176 1105 6 0.044023948166774574 1105 7 0.023525239951595722 1105 8 0.09516030693627897 1105 9 0.2429409287364308 1105 10-11 0.17328694073616902 1105 12-13 0.18661982471604688 1105 14-15 0.17622767349321578 1105 16-17 0.18184624627802037 1105 18-19 0.20112697672351487 1105 20-21 0.23383615142842729 1105 22-23 0.16347402921520882 1105 24-25 0.12726432758867645 1105 26-27 0.1482581217168928 1105 28-29 0.22175851967774207 1105 30-31 0.2081417585761045 1105 32-33 0.2808589117099274 1105 34-35 0.46454640583203144 1105 36-37 0.4177870346115782 1105 38-39 0.4026542840937566 1105 40-41 0.38088101603180036 1105 42-43 0.37680984418353347 1105 44-45 0.49243956984212645 1105 46-47 0.6084872547148805 1105 48-49 0.5142064266607065 1105 50-51 0.5042830176557729 1105 52-53 0.409008244235757 1105 54-55 0.4709496933360384 1105 56-57 0.5176881994757352 1105 58-59 0.35782670322743826 1105 60-61 0.4898819098284406 1105 62-63 0.7129825207315648 1105 64-65 0.6704179971081388 1105 66-67 0.8164760025366036 1105 68-69 0.7489141265484385 1105 70-71 0.7681496898122475 1105 72-73 0.6815861307510822 1105 74-75 0.6908064132816811 1105 76-77 0.6267638111906635 1105 78-79 0.9023546238691864 1105 80-81 1.1657242344317318 1105 82-83 1.0955925738183474 1105 84-85 1.2895194623282684 1105 86-87 1.6111532928334888 1105 88-89 1.5527701118356134 1105 90-91 1.559333057026688 1105 92-93 1.800822190113898 1105 94-95 1.938016414986798 1105 96-97 1.757108976463524 1105 98-99 1.8507420082166384 1105 100-101 2.616462525047119 1106 1 -0.1610476464720314 1106 2 -0.032189859958851486 1106 3 0.033861100745649964 1106 4 0.01108747887226258 1106 5 -0.1035146802180833 1106 6 -0.006253558233979106 1106 7 -0.005189724338933388 1106 8 0.014234976792096177 1106 9 0.04672200608449373 1106 10-11 -0.3224872979109037 1106 12-13 -0.3957667303304433 1106 14-15 -0.4378984129506591 1106 16-17 -0.30503010124926533 1106 18-19 0.04658981023651876 1106 20-21 -0.07500973356011542 1106 22-23 -0.13424949187358948 1106 24-25 -0.37352839744838207 1106 26-27 -0.3724790599297805 1106 28-29 -0.08737266210368233 1106 30-31 0.03203257517763802 1106 32-33 -0.007323484530466828 1106 34-35 -0.03656425142440867 1106 36-37 -0.18960773192931413 1106 38-39 -0.2589584534079279 1106 40-41 -0.17962822295216796 1106 42-43 -0.0819705594008937 1106 44-45 0.06308368788551633 1106 46-47 0.3334758849533941 1106 48-49 0.2410121132258567 1106 50-51 0.15087503692541304 1106 52-53 0.25734720630286034 1106 54-55 0.3527178596882834 1106 56-57 0.2001276028580321 1106 58-59 -0.11862736293614518 1106 60-61 0.06178091265825003 1106 62-63 -0.13838732497656991 1106 64-65 -0.008045148020549675 1106 66-67 0.2764354924975265 1106 68-69 0.12518374991455872 1106 70-71 0.06012621757130887 1106 72-73 0.05203401513324124 1106 74-75 0.2659457981354727 1106 76-77 0.500983719221928 1106 78-79 0.4991344547545111 1106 80-81 0.45652693959275936 1106 82-83 0.44387995085648413 1106 84-85 0.5336135028888407 1106 86-87 0.7485789682815422 1106 88-89 0.7977874191392083 1106 90-91 0.8845492930460956 1106 92-93 0.7022705713967454 1106 94-95 0.9012594499551305 1106 96-97 1.0653642655081264 1106 98-99 1.0461039877342238 1106 100-101 1.4383815302350982 1107 1 -0.0015897450115147649 1107 2 0.09096411025098661 1107 3 0.06542651135703892 1107 4 0.11429017067815295 1107 5 0.11597656303202086 1107 6 0.13468288850975796 1107 7 0.15009988715673472 1107 8 0.12580256186802075 1107 9 0.15419321708003508 1107 10-11 0.16249247503875353 1107 12-13 0.21252556753495355 1107 14-15 0.33898765190274815 1107 16-17 0.16915209622835192 1107 18-19 0.36629074208517665 1107 20-21 0.33986932317528584 1107 22-23 0.3177106973048254 1107 24-25 0.28404921508015946 1107 26-27 0.31646548669436214 1107 28-29 0.300094267914794 1107 30-31 0.3092439603115551 1107 32-33 0.2606999385297257 1107 34-35 0.32211112593579827 1107 36-37 0.2695783048372533 1107 38-39 0.4406636201252141 1107 40-41 0.3619054653927023 1107 42-43 0.3463938582050261 1107 44-45 0.44685176312199104 1107 46-47 0.5692608893023205 1107 48-49 0.5412694248628682 1107 50-51 0.3889386989049015 1107 52-53 0.4742593873814016 1107 54-55 0.5024113394657661 1107 56-57 0.5224860248309895 1107 58-59 0.5137380694198797 1107 60-61 0.7291082239328475 1107 62-63 0.7569105087131405 1107 64-65 0.7743962773409478 1107 66-67 0.9170797455689552 1107 68-69 0.9274518749825127 1107 70-71 1.0541767353656724 1107 72-73 1.0000914934792 1107 74-75 1.0142181181079302 1107 76-77 1.0174453784289739 1107 78-79 1.167106743922048 1107 80-81 1.3333690055152765 1107 82-83 1.468616183777879 1107 84-85 1.4533389540902348 1107 86-87 1.546035807425529 1107 88-89 1.655739673787128 1107 90-91 1.8310736040126407 1107 92-93 1.7932721954499442 1107 94-95 1.9424879204911356 1107 96-97 2.1727930064931584 1107 98-99 2.2720263231091415 1107 100-101 2.872976864649626 1108 1 0.007956262700496097 1108 2 -0.05770235434759741 1108 3 0.007022947969950621 1108 4 0.028606276567906264 1108 5 0.013828298234706438 1108 6 0.06832232460306642 1108 7 0.058614891851398454 1108 8 0.11913032693424697 1108 9 0.19501571774093662 1108 10-11 0.10270301706739104 1108 12-13 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-0.7058500073945666 2316 62-63 -0.6278680201857156 2316 64-65 -0.8950946821394865 2316 66-67 -0.5194857904193384 2316 68-69 -0.5341854888557158 2316 70-71 -0.6531792257008213 2316 72-73 -0.689476735010011 2316 74-75 -0.8248017447779041 2316 76-77 -0.8034133836580644 2316 78-79 -0.9141835620122265 2316 80-81 -1.0384378234805602 2316 82-83 -1.042021775021091 2316 84-85 -1.3736598939322064 2316 86-87 -1.5771813972606985 2316 88-89 -1.5691579535033569 2316 90-91 -1.6237945636145135 2316 92-93 -1.9648512194425862 2316 94-95 -2.1055705268131 2316 96-97 -1.8864321482042214 2316 98-99 -2.015061586893939 2316 100-101 -2.2765872734844734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10119.0 3 2225.0 4 2290.0 5 2444.0 6 2777.0 7 3308.0 8 3992.0 9 5103.0 10 6570.0 11 7938.0 12 10397.0 13 12977.0 14 14645.0 15 17607.0 16 21497.0 17 27584.0 18 24121.0 19 18407.0 20 18307.0 21 19595.0 22 21076.0 23 23267.0 24 26158.0 25 29553.0 26 34050.0 27 39293.0 28 45926.0 29 54125.0 30 64746.0 31 78918.0 32 98131.0 33 124395.0 34 163834.0 35 222372.0 36 306986.0 37 385107.0 38 243198.0 39 20113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14954532801757 19.196505885030415 15.067418756114655 22.58653003083736 2 14.975796596253332 19.905832605698166 41.51929316908036 23.59907762896814 3 18.751515211934816 24.731793326678105 30.1285080979723 26.388183363414775 4 12.434173859851832 16.725073064636717 37.590475543170434 33.250277532341016 5 13.312686484318892 37.54104637384994 34.53754154479815 14.608725597033015 6 31.390270983026657 36.389718547874395 17.71034647645992 14.509663992639027 7 28.08748894661276 33.14232235005087 20.832301382007326 17.93788732132904 8 25.908974415864684 35.49036938561308 19.795125874018215 18.805530324504023 9 26.126841240157034 17.035586366785456 20.804734585203065 36.03283780785445 10-11 24.521342579584545 27.173500338821416 27.293519873410073 21.01163720818397 12-13 25.41312836424579 24.47169425987838 28.139460851239374 21.975716524636457 14-15 22.591360621903366 25.761689441353784 26.504315704345956 25.142634232396897 16-17 21.46245497744061 29.13995875243082 27.22117707250924 22.176409197619325 18-19 21.444802262265824 27.43196518950234 29.991909484013423 21.131323064218414 20-21 22.234757844302592 26.48902704230976 30.17889897146277 21.09731614192487 22-23 21.936393363053437 26.37607160353635 30.105460186198897 21.58207484721132 24-25 21.645880138112016 26.59131719853195 30.162493514114104 21.600309149241934 26-27 21.34075730789006 26.749242309500865 30.182060795903865 21.727939586705215 28-29 21.42172740243915 26.900127740060526 30.179189283057212 21.498955574443112 30-31 21.539439659124948 26.703962045295594 30.167211085910917 21.589387209668544 32-33 21.32972374269599 26.76917336540517 30.097976974935424 21.803125916963413 34-35 21.706401155242766 26.77401446408138 29.99340689296661 21.526177487709244 36-37 21.79324271246835 26.489160245896535 29.91477835467553 21.802818686959583 38-39 21.412485329053624 26.829004390248873 29.975541820544194 21.782968460153313 40-41 21.74386415234422 26.903838710334572 29.808822763808063 21.543474373513146 42-43 21.542042407773458 26.773268632970925 29.745226476924007 21.939462482331603 44-45 21.636387550054888 26.99170847292333 29.64064163520586 21.731262341815924 46-47 21.7965688588362 27.253436676430926 29.039414950564062 21.910579514168813 48-49 21.83760721496154 27.276065832697675 28.915973900035567 21.97035305230521 50-51 22.02381909208701 27.493656717178222 28.650877078985896 21.831647111748868 52-53 21.93265057049115 27.51313861836757 28.609310361224267 21.94490044991701 54-55 21.94966068790645 27.492282701592135 28.575907992370432 21.98214861813098 56-57 21.91721540205727 27.785049485207626 28.474155360940873 21.82357975179423 58-59 21.938850327500244 27.397957151598757 28.631016391305764 22.032176129595232 60-61 22.013003253642665 27.433651152921207 28.68855000707314 21.86479558636299 62-63 21.802356766866755 27.498004945652593 28.866614491223796 21.833023796256857 64-65 21.89791215539179 27.338014534311945 28.793279097312713 21.970794212983556 66-67 21.87949498010807 27.46419689990523 28.60486229106795 22.05144582891875 68-69 21.788443257435155 27.465347879226837 28.676723552577972 22.069485310760037 70-71 21.82324937535691 27.438348819170493 28.63120832081859 22.107193484654005 72-73 21.858046202722907 27.396193272298625 28.45199034340229 22.293770181576182 74-75 21.759848888554856 27.79090515194225 28.280514202691993 22.168731756810903 76-77 21.900382302179903 27.541331556138292 28.339371510428645 22.21891463125316 78-79 21.904361128553894 27.49388924076278 28.309419239055977 22.292330391627342 80-81 21.723973855806726 27.797627428494852 28.241709287327616 22.236689428370802 82-83 22.040366234902045 27.72643650688026 28.0877417415623 22.145455516655396 84-85 21.879284676085845 27.70947695096056 28.066215488907424 22.34502288404617 86-87 21.907691806264875 27.56263020137813 28.211000245351226 22.318677747005765 88-89 22.02014453888871 27.449056345810437 28.161270395740118 22.369528719560737 90-91 21.938301441477044 27.50257531917321 28.23799276533936 22.321130474010385 92-93 21.910629251065547 27.346425217573756 28.31352530025529 22.42942023110541 94-95 21.774433217528173 27.46741607671977 28.236827398856462 22.521323306895592 96-97 21.843703433243913 27.390007100952822 28.130638517587254 22.635650948216014 98-99 21.76554581598274 27.485175597525963 28.047706099713064 22.701572486778236 100-101 22.28153540240197 26.69720011714271 28.55853808790321 22.462726392552113 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2970.0 1 1747.0 2 499.0 3 474.0 4 426.5 5 447.0 6 733.5 7 1442.0 8 2407.5 9 2987.0 10 2872.5 11 2685.5 12 2806.5 13 3040.5 14 3255.0 15 3399.5 16 3545.0 17 3748.5 18 3914.5 19 4198.0 20 4715.0 21 5554.0 22 6599.0 23 7902.5 24 9665.0 25 11884.5 26 15030.5 27 18660.5 28 21352.5 29 23871.0 30 27489.0 31 32035.5 32 36773.0 33 40994.5 34 45651.5 35 50129.0 36 55925.0 37 61954.5 38 64302.5 39 65529.5 40 67822.0 41 71857.0 42 74775.5 43 77141.5 44 80997.5 45 83311.5 46 84188.5 47 85823.0 48 87482.0 49 85998.5 50 82001.5 51 76803.5 52 72483.0 53 70677.5 54 68918.0 55 63498.0 56 56083.0 57 49941.0 58 44888.0 59 39476.0 60 32320.0 61 25781.0 62 20312.0 63 15036.5 64 11093.0 65 8472.5 66 6371.0 67 4706.0 68 3557.0 69 2684.0 70 1934.0 71 1292.5 72 898.5 73 683.5 74 530.5 75 386.0 76 248.5 77 165.0 78 108.0 79 75.0 80 54.0 81 32.0 82 23.0 83 18.0 84 13.5 85 9.5 86 5.0 87 7.5 88 9.0 89 4.0 90 3.5 91 6.0 92 5.0 93 2.0 94 1.0 95 1.0 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33368712753897045 2 0.2814990933741078 3 0.28755380902613514 4 0.28018874446434067 5 0.2799628222385187 6 0.2144905611953274 7 0.20567959438827266 8 0.211146912253163 9 0.21331576562105345 10-11 0.21510055120504656 12-13 0.21769865680199862 14-15 0.2125928144984233 16-17 0.2049566432656425 18-19 0.19845008316197132 20-21 0.19585197756501926 22-23 0.205363303272122 24-25 0.20373666324620415 26-27 0.20922657333367675 28-29 0.21507795898246437 30-31 0.21844420014721094 32-33 0.21575572565993012 34-35 0.21616238566640958 36-37 0.20326222657197812 38-39 0.19332164863581383 40-41 0.19004577636139605 42-43 0.1990374809491083 44-45 0.20109337320408774 46-47 0.20719327330127948 48-49 0.2166820067858 50-51 0.21293169783715618 52-53 0.22438595468632733 54-55 0.2095880488949918 56-57 0.18329070180932075 58-59 0.16659504932108113 60-61 0.184736604054581 62-63 0.17793634505734132 64-65 0.18536918628688237 66-67 0.1847817884997454 68-69 0.18304218736091665 70-71 0.1847817884997454 72-73 0.19422533753910148 74-75 0.20107078098150558 76-77 0.20003153874272472 78-79 0.19619086090375215 80-81 0.19537754089079326 82-83 0.1842169829351906 84-85 0.17938224730260158 86-87 0.18403624515453304 88-89 0.19268906640351247 90-91 0.1898424463581563 92-93 0.18401365293195085 94-95 0.1863632440804988 96-97 0.1939542308681152 98-99 0.1939768230906974 100-101 0.1753156472378071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2213151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.69796039622911 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43697425479276 40.508116559452155 2 13.460534911353891 14.456065411638807 3 4.619125310902293 7.441128240301524 4 2.112603226292066 4.537699375135087 5 1.145414836296998 3.0753220258364715 6 0.7016735952631619 2.260706455771257 7 0.44476125268174194 1.6717940492596999 8 0.34216496807873487 1.469884872389512 9 0.2487459848412613 1.2021436838454347 >10 1.3329368763994078 13.105879890702926 >50 0.10449563790178884 3.874738235038454 >100 0.04854466473035491 4.509760369369039 >500 0.001434006996457236 0.4852110727530385 >1k 3.374134109310865E-4 0.34207561813206705 >5k 1.6870670546554326E-4 0.6064685449113144 >10k+ 8.435335273277163E-5 0.45300559546329483 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10001 0.4518896360889971 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8300 0.3750308948643811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5089 0.22994364144154647 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3181 0.1437317200679032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.518444516438327E-5 0.0 3 0.0 0.0 0.0 4.518444516438327E-5 0.0 4 0.0 0.0 0.0 4.518444516438327E-5 0.0 5 0.0 0.0 0.0 4.518444516438327E-5 0.0 6 0.0 0.0 0.0 4.518444516438327E-5 0.0 7 0.0 0.0 0.0 4.518444516438327E-5 0.0 8 0.0 0.0 0.0 4.518444516438327E-5 0.0 9 0.0 0.0 0.0 2.7110667098629963E-4 0.0 10-11 0.0 0.0 0.0 2.7110667098629963E-4 0.0 12-13 0.0 0.0 2.2592222582191634E-5 2.7110667098629963E-4 0.0 14-15 0.0 0.0 4.518444516438327E-5 2.7110667098629963E-4 0.0 16-17 0.0 0.0 4.518444516438327E-5 3.162911161506829E-4 0.0 18-19 0.0 0.0 4.518444516438327E-5 3.162911161506829E-4 0.0 20-21 0.0 0.0 4.518444516438327E-5 3.840677838972578E-4 0.0 22-23 0.0 0.0 4.518444516438327E-5 4.0666000647944945E-4 0.0 24-25 0.0 0.0 4.518444516438327E-5 4.7443667422602436E-4 0.0 26-27 0.0 0.0 4.518444516438327E-5 5.873977871369825E-4 0.0 28-29 0.0 0.0 4.518444516438327E-5 9.714655710342404E-4 0.0 30-31 0.0 0.0 4.518444516438327E-5 0.001920338919486289 0.0 32-33 0.0 0.0 4.518444516438327E-5 0.003343648942164362 0.0 34-35 0.0 0.0 4.518444516438327E-5 0.005444725642308184 0.0 36-37 0.0 0.0 4.518444516438327E-5 0.007410249006958856 0.0 38-39 0.0 0.0 4.518444516438327E-5 0.009127257923205422 0.0 40-41 0.0 0.0 4.518444516438327E-5 0.01204165463630814 0.0 42-43 0.0 0.0 4.518444516438327E-5 0.016966759159225915 0.0 44-45 0.0 0.0 4.518444516438327E-5 0.023292581482239574 0.0 46-47 0.0 0.0 4.518444516438327E-5 0.037277167260616195 0.0 48-49 0.0 0.0 4.518444516438327E-5 0.06330340767530096 0.0 50-51 0.0 0.0 4.518444516438327E-5 0.08487898024129398 0.0 52-53 0.0 0.0 4.518444516438327E-5 0.10453421388780071 0.0 54-55 0.0 0.0 4.518444516438327E-5 0.13413002547047176 0.0 56-57 0.0 0.0 4.518444516438327E-5 0.15699335472364967 0.0 58-59 0.0 0.0 4.518444516438327E-5 0.18575325407077964 0.0 60-61 0.0 0.0 4.518444516438327E-5 0.23211249480943685 0.0 62-63 0.0 0.0 4.518444516438327E-5 0.2661363820182175 0.0 64-65 0.0 0.0 4.518444516438327E-5 0.3068249748887446 0.0 66-67 0.0 0.0 4.518444516438327E-5 0.3778775149097373 0.0 68-69 0.0 0.0 4.518444516438327E-5 0.45905137064755186 0.0 70-71 0.0 0.0 4.518444516438327E-5 0.5326794240429144 0.0 72-73 0.0 0.0 4.518444516438327E-5 0.6158413953679618 0.0 74-75 0.0 0.0 4.518444516438327E-5 0.6954337955250229 0.0 76-77 0.0 0.0 4.518444516438327E-5 0.7683163055751732 0.0 78-79 0.0 2.2592222582191634E-5 4.518444516438327E-5 0.8556352458553438 0.0 80-81 0.0 4.518444516438327E-5 4.518444516438327E-5 0.9573002474752061 0.0 82-83 0.0 4.518444516438327E-5 4.518444516438327E-5 1.0843137228322877 4.518444516438327E-5 84-85 0.0 4.518444516438327E-5 4.518444516438327E-5 1.230688732942307 4.518444516438327E-5 86-87 0.0 4.518444516438327E-5 4.518444516438327E-5 1.4469640797216277 4.518444516438327E-5 88-89 0.0 4.518444516438327E-5 4.518444516438327E-5 1.7299316675635779 4.518444516438327E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 51.5983 1 GTATCAA 9585 0.0 42.01614 1 ATCAACG 12250 0.0 32.669567 3 TATCAAC 12420 0.0 32.29894 2 TCAACGC 12505 0.0 32.083027 4 CAACGCA 12755 0.0 31.452772 5 AACGCAG 13270 0.0 30.286594 6 CGCAGAG 14930 0.0 26.632786 8 ACGCAGA 15130 0.0 26.40633 7 GCAGAGT 15645 0.0 24.777962 9 GTACATG 9240 0.0 24.545465 1 TACATGG 9290 0.0 24.046371 2 GAGTACT 9150 0.0 23.722878 12-13 ACATGGG 9450 0.0 22.583015 3 GTACTTT 9815 0.0 22.041485 14-15 CAGAGTA 15455 0.0 21.114206 10-11 AGTACTT 9665 0.0 21.107344 12-13 AGAGTAC 15020 0.0 20.44493 10-11 TGGTATC 2720 0.0 20.095385 2 GTATAGG 640 0.0 20.058971 1 >>END_MODULE