FastQCFastQC Report
Thu 26 May 2016
SRR937876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213151
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117190.5295165128814076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105730.47773513872302437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.3097845560470117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.11671142185960198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61050.051.6074751
GTATCAA101550.039.8230361
ATCAACG129150.030.8974323
TCAACGC131550.030.333744
TATCAAC133750.030.0834122
CAACGCA133500.029.9262455
AACGCAG139250.028.7548436
CGCAGAG160350.024.7654028
ACGCAGA160700.024.7410247
GTACATG92800.023.2483731
GAGTACT103250.023.1642112-13
GCAGAGT170600.022.720579
TACATGG94850.022.2373562
GTACTTT107350.022.0567614-15
ACATGGG92200.021.4338383
TGGTATC30250.021.043452
AGTACTT105950.020.84779212-13
CAGAGTA167800.020.4245510-11
GTGGTAT34650.019.6118031
AGAGTAC162100.019.60429210-11
Produced by FastQC (version 0.11.5)