FastQCFastQC Report
Thu 26 May 2016
SRR937875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518972
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.46854056559304585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.3937531435734168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37440.2464824894731437No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20510.13502553042452395No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.10263520328221983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.046.643231
GTATCAA71250.037.0458451
ATCAACG88650.029.2820783
TCAACGC89400.028.9842014
CAACGCA90850.028.5739355
TATCAAC91250.028.551942
AACGCAG93850.027.6883476
ACGCAGA105450.024.7325977
CGCAGAG106950.024.1628288
GAGTACT62850.023.1284112-13
GCAGAGT111100.022.5753869
ATAACGC1503.0128362E-522.1868063
GTACATG64900.021.7642231
GTACTTT68100.021.45001814-15
TACATGG65250.021.2031562
GTACCTG12000.021.0051611
TAACGCA1604.9545844E-520.8008174
CAGAGTA108250.020.58104510-11
ACATGGG66050.020.2985153
TACCTGG12000.020.2058412
Produced by FastQC (version 0.11.5)