FastQCFastQC Report
Thu 26 May 2016
SRR937875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518972
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82880.5456321775516599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.5145585303744901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.3291041572853219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.12824462860408223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.046.84721
GTATCAA74050.037.1556131
ATCAACG94150.029.0670913
TATCAAC94650.028.9135422
TCAACGC95050.028.7918644
CAACGCA96250.028.3835375
AACGCAG98750.027.6567736
ACGCAGA112350.024.3519887
CGCAGAG112700.024.2342158
GTACATG58400.023.3528541
GAGTACT73150.022.85669112-13
GCAGAGT119300.022.6944399
GTACCTG12100.022.3851721
TACATGG60800.021.8021852
GTACTTT76550.021.59259214-15
TACCTGG12550.021.2003842
AGTACTT75600.021.0792812-13
ACATGGG60050.020.9668063
CAGAGTA117400.019.98682810-11
AGAGTAC110750.019.70728510-11
Produced by FastQC (version 0.11.5)