##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937875_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1518972 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.027045923163826 31.0 31.0 34.0 30.0 34.0 2 32.318159255075145 33.0 31.0 34.0 31.0 34.0 3 32.40167099854375 34.0 31.0 34.0 31.0 34.0 4 36.06269832491975 37.0 35.0 37.0 35.0 37.0 5 35.961810355951265 37.0 35.0 37.0 35.0 37.0 6 36.00002567525932 37.0 35.0 37.0 35.0 37.0 7 35.94037743947881 37.0 35.0 37.0 35.0 37.0 8 35.91700044503783 37.0 35.0 37.0 35.0 37.0 9 37.5983533600356 39.0 37.0 39.0 35.0 39.0 10-11 37.506996837334725 39.0 37.0 39.0 35.0 39.0 12-13 37.38845877343361 39.0 37.0 39.0 34.0 39.0 14-15 38.56992591041836 40.0 38.0 41.0 34.0 41.0 16-17 38.51915045175289 40.0 38.0 41.0 34.0 41.0 18-19 38.50356227764567 40.0 38.0 41.0 34.0 41.0 20-21 38.40655423536444 40.0 38.0 41.0 34.0 41.0 22-23 38.34629209755019 40.0 38.0 41.0 34.0 41.0 24-25 38.37182449709408 40.0 38.0 41.0 34.0 41.0 26-27 38.223356651735514 40.0 38.0 41.0 34.0 41.0 28-29 38.062976144392394 40.0 37.5 41.0 33.0 41.0 30-31 38.09932737403981 40.0 37.0 41.0 34.0 41.0 32-33 38.009397474081155 40.0 37.0 41.0 33.0 41.0 34-35 37.85991479763946 40.0 37.0 41.0 33.0 41.0 36-37 37.702878986577765 40.0 37.0 41.0 33.0 41.0 38-39 37.5639086829777 40.0 37.0 41.0 33.0 41.0 40-41 37.37137978843586 40.0 36.5 41.0 32.5 41.0 42-43 37.14098153224681 40.0 36.0 41.0 31.5 41.0 44-45 36.83941672394224 39.5 36.0 41.0 31.0 41.0 46-47 36.45859403596643 39.0 36.0 41.0 30.0 41.0 48-49 36.111582043645306 39.0 35.0 41.0 29.5 41.0 50-51 35.84283054592184 39.0 35.0 40.0 28.5 41.0 52-53 35.597842817379124 39.0 35.0 40.0 28.0 41.0 54-55 35.356174109858514 38.5 35.0 40.0 27.5 41.0 56-57 35.068765915369084 38.0 34.0 40.0 27.0 41.0 58-59 34.80225211524636 38.0 34.0 40.0 26.0 41.0 60-61 34.46546644704445 38.0 34.0 40.0 26.0 41.0 62-63 34.073426304105666 37.0 33.5 40.0 25.0 41.0 64-65 33.6514461754397 36.5 33.0 39.5 23.5 41.0 66-67 33.208034446981245 36.0 32.5 39.0 22.5 40.0 68-69 32.75320414069516 35.5 32.0 39.0 22.0 40.0 70-71 32.33989303291963 35.0 31.0 38.0 21.0 40.0 72-73 31.82715711678688 35.0 31.0 37.5 19.5 39.5 74-75 31.32729503901323 35.0 30.5 37.0 18.0 39.0 76-77 30.122328785520736 33.5 29.5 35.5 18.0 38.0 78-79 30.722998514784997 34.0 30.0 36.0 18.0 38.0 80-81 30.520101094687725 34.0 30.0 36.0 16.0 37.5 82-83 30.12223464290323 34.0 30.0 35.0 10.0 37.0 84-85 29.70496789934245 34.0 29.5 35.0 7.0 36.5 86-87 29.27958876134649 34.0 29.0 35.0 3.5 36.0 88-89 28.795632506721653 34.0 29.0 35.0 2.0 36.0 90-91 28.192105911103035 33.0 27.5 35.0 2.0 35.0 92-93 27.69348875423642 33.0 27.0 35.0 2.0 35.0 94-95 27.14482393355506 33.0 26.0 35.0 2.0 35.0 96-97 26.48589835757341 32.5 25.0 34.0 2.0 35.0 98-99 25.48248716895374 32.0 22.0 34.0 2.0 35.0 100-101 22.645217950034628 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2599728965965582 1101 2 0.1854351287929532 1101 3 0.1896330919345104 1101 4 0.04475480532538256 1101 5 0.08375207043471988 1101 6 0.07858245541095243 1101 7 0.06940612473876229 1101 8 0.06032880653604167 1101 9 0.08202229154501595 1101 10-11 0.24017052216273527 1101 12-13 0.19611209807386132 1101 14-15 0.13679587246257086 1101 16-17 0.21134800609777216 1101 18-19 0.264756240807813 1101 20-21 0.3279362456728947 1101 22-23 0.17327607387680644 1101 24-25 0.22974264858351034 1101 26-27 0.020940903015791434 1101 28-29 0.0511752795677225 1101 30-31 0.11095721113422741 1101 32-33 0.23657091225550886 1101 34-35 0.2911589755931203 1101 36-37 0.15776807632656897 1101 38-39 0.07251150958365571 1101 40-41 0.1454108696531975 1101 42-43 0.08730514260384581 1101 44-45 -0.013762507989063977 1101 46-47 -0.055552936382945006 1101 48-49 -0.01877708138267309 1101 50-51 0.10986500960027001 1101 52-53 0.25755493926096307 1101 54-55 0.09082022708494719 1101 56-57 -0.02050769395189178 1101 58-59 0.005827141666230773 1101 60-61 0.07318211334656155 1101 62-63 -0.16915615145148877 1101 64-65 0.04678462160955377 1101 66-67 0.04613381454564802 1101 68-69 0.08497372953731741 1101 70-71 -0.1275994248112795 1101 72-73 -0.16167070406368111 1101 74-75 -0.0906429409958065 1101 76-77 -0.0030786466777428245 1101 78-79 -0.01021260802763635 1101 80-81 -0.12626898490604432 1101 82-83 0.03744758000716786 1101 84-85 0.04240627415128628 1101 86-87 0.07051837657726168 1101 88-89 0.15656620529238907 1101 90-91 0.0820532788106938 1101 92-93 -0.054537617732236754 1101 94-95 -0.10078072477845268 1101 96-97 -0.00786807800995959 1101 98-99 -0.07272188226460585 1101 100-101 -0.003725939040332804 1102 1 0.3335272751788665 1102 2 0.20460487896828994 1102 3 0.23226713387022357 1102 4 0.16752560993975862 1102 5 0.04955112240695314 1102 6 0.05141130254147441 1102 7 0.07465957705925774 1102 8 0.1251635062869383 1102 9 0.19078187460628016 1102 10-11 0.14984880942481738 1102 12-13 0.18294007456664474 1102 14-15 0.3097027142241586 1102 16-17 0.39061443333906 1102 18-19 0.24031792307340538 1102 20-21 0.11624823628935133 1102 22-23 0.22762412340642157 1102 24-25 0.1052103140651397 1102 26-27 0.19397078281941305 1102 28-29 0.11893296415501453 1102 30-31 0.19116622465329414 1102 32-33 0.2361661261663599 1102 34-35 0.2425220678081743 1102 36-37 0.06183256398043824 1102 38-39 0.14095935596446196 1102 40-41 0.18190661320200974 1102 42-43 0.1807091547927797 1102 44-45 -4.6593491595814385E-4 1102 46-47 -0.08643548493309083 1102 48-49 2.790021985532576E-4 1102 50-51 0.06038920607418419 1102 52-53 0.20780554991659983 1102 54-55 0.09552953081659865 1102 56-57 0.06816555086660259 1102 58-59 0.07680104671835153 1102 60-61 0.021192645644809716 1102 62-63 -0.08410995928239373 1102 64-65 -0.08707925659790305 1102 66-67 0.009552518746467342 1102 68-69 0.10037101846813101 1102 70-71 -0.03587894789527013 1102 72-73 -0.0882435957902068 1102 74-75 -0.10827486679811926 1102 76-77 -0.16326268982268033 1102 78-79 -0.029673477698000994 1102 80-81 -0.17418429120123946 1102 82-83 -0.08142288844430468 1102 84-85 0.009317203073386793 1102 86-87 -0.1580717948900059 1102 88-89 -0.11798275598841812 1102 90-91 0.2800087916405083 1102 92-93 0.18879928279740454 1102 94-95 -0.13992556504061326 1102 96-97 -0.4841199220690662 1102 98-99 -0.24332334783813891 1102 100-101 -0.16366783606323665 1103 1 0.2640110084418623 1103 2 0.23059336580659107 1103 3 0.13223988960193367 1103 4 0.05108846611084772 1103 5 0.127537695783694 1103 6 0.0978862065791759 1103 7 0.0034482078120774418 1103 8 0.07557951643037342 1103 9 0.11329825008898098 1103 10-11 0.12967662680608072 1103 12-13 0.21371568591413848 1103 14-15 0.23015899498241765 1103 16-17 0.2145228983819507 1103 18-19 0.032540176145467115 1103 20-21 -0.004793391332988506 1103 22-23 -0.06915604947609921 1103 24-25 0.04873123951418279 1103 26-27 0.006302747576548029 1103 28-29 -0.05389012625894196 1103 30-31 0.012054972018525234 1103 32-33 -0.04595449016724018 1103 34-35 0.06736788607328492 1103 36-37 0.10093054732615059 1103 38-39 0.024305877606010995 1103 40-41 -0.0714704324318447 1103 42-43 0.03952007262961388 1103 44-45 -0.0989802835639253 1103 46-47 0.09739637223520958 1103 48-49 0.00918111024550683 1103 50-51 -0.1603752646234753 1103 52-53 0.05592584001310286 1103 54-55 0.006087060762155261 1103 56-57 0.05678658598683484 1103 58-59 -0.15677314677032683 1103 60-61 -0.06963474138113668 1103 62-63 -0.22292239840215444 1103 64-65 -0.03156934917057441 1103 66-67 0.019730075022373228 1103 68-69 -0.07964606270475372 1103 70-71 -0.1852492640213299 1103 72-73 -0.21148587773772576 1103 74-75 -0.1631589080196818 1103 76-77 -0.09544239324770487 1103 78-79 0.018694498081785582 1103 80-81 0.06609222366105882 1103 82-83 0.01867666014535274 1103 84-85 -0.07585555219556994 1103 86-87 -0.09492611730018297 1103 88-89 -0.10934990923424337 1103 90-91 -0.06011608659901313 1103 92-93 -0.10574466203382116 1103 94-95 -0.3684783680188559 1103 96-97 -0.39544604695077723 1103 98-99 -0.3512332092220767 1103 100-101 -0.41375821646494515 1104 1 0.23802418671803593 1104 2 0.1710128841086842 1104 3 0.1700951509235935 1104 4 0.06699585800155461 1104 5 0.02842846572092128 1104 6 0.10454830638270352 1104 7 0.100131171192821 1104 8 0.11219877149122226 1104 9 0.0659513061137389 1104 10-11 0.12914562763008774 1104 12-13 0.08604049527269808 1104 14-15 0.1039735841992524 1104 16-17 0.10257530110978763 1104 18-19 0.12572242467508943 1104 20-21 0.12843528632851076 1104 22-23 0.16701249325253542 1104 24-25 0.10625475084901836 1104 26-27 0.08310248445111057 1104 28-29 0.09419567093180348 1104 30-31 0.1288866614934534 1104 32-33 0.16217995975060973 1104 34-35 0.23634954314972845 1104 36-37 0.07479114296014444 1104 38-39 0.04904540137444968 1104 40-41 0.04837787668031268 1104 42-43 0.08974466153954808 1104 44-45 0.006085025860350868 1104 46-47 -0.10235471828190157 1104 48-49 -0.15214955515958195 1104 50-51 -0.23417010785131964 1104 52-53 -0.2231065544109967 1104 54-55 -0.255059434208988 1104 56-57 -0.07853047611110497 1104 58-59 -0.1043183034150914 1104 60-61 -0.171459479225625 1104 62-63 -0.07035669376855935 1104 64-65 -0.28206531514030075 1104 66-67 -0.4266099103737062 1104 68-69 -0.262773173021138 1104 70-71 -0.23302804293169288 1104 72-73 -0.3095876859723212 1104 74-75 -0.2218370675082113 1104 76-77 -0.36486859565393814 1104 78-79 -0.23400465121931902 1104 80-81 -0.22312947747036915 1104 82-83 -0.2713063617784819 1104 84-85 -0.17495392485971095 1104 86-87 -0.06426829791749356 1104 88-89 -0.2088326338101254 1104 90-91 -0.22627395143800655 1104 92-93 -0.1905124266199394 1104 94-95 -0.2636774000600539 1104 96-97 -0.24248860737437283 1104 98-99 0.0018082810963058193 1104 100-101 -0.2113422860264187 1105 1 0.1948132284893589 1105 2 0.1165972832919806 1105 3 0.07434147057646356 1105 4 0.029548556318538033 1105 5 0.044647794650870765 1105 6 0.07399914414772013 1105 7 0.019202508565378196 1105 8 0.053688457818026336 1105 9 0.12272620839110004 1105 10-11 0.04959949330126534 1105 12-13 0.04846871770264727 1105 14-15 0.052541930222879785 1105 16-17 -0.07706908457451078 1105 18-19 0.03594509753493469 1105 20-21 -0.18022382436982554 1105 22-23 -0.09359696123490835 1105 24-25 -0.15830288541431514 1105 26-27 -0.12113193143974144 1105 28-29 -0.09650969645635854 1105 30-31 -0.026832673176890864 1105 32-33 -0.020335679798641593 1105 34-35 0.1125174864999039 1105 36-37 0.11737760687210397 1105 38-39 0.1367697827564811 1105 40-41 0.08913678078494058 1105 42-43 0.0989676600911551 1105 44-45 0.12212721835204121 1105 46-47 0.03981109568465513 1105 48-49 -0.010755975094092207 1105 50-51 0.04641621587420275 1105 52-53 0.24252223915783588 1105 54-55 0.1369970101675122 1105 56-57 0.22626716295594917 1105 58-59 0.2619522962867791 1105 60-61 0.14459748722450172 1105 62-63 0.046082190081541796 1105 64-65 0.12738406637769373 1105 66-67 0.11912546114150047 1105 68-69 0.19322384560009453 1105 70-71 -0.0462101300055906 1105 72-73 0.0486595062202575 1105 74-75 -0.1481438679401279 1105 76-77 -0.31107295726484807 1105 78-79 -0.11451565494815341 1105 80-81 -0.2328582348445103 1105 82-83 -0.25218949402686164 1105 84-85 0.005260421409246874 1105 86-87 -0.048236589195905566 1105 88-89 -0.04882160017081816 1105 90-91 0.12569007865615234 1105 92-93 0.14292146429688302 1105 94-95 -0.11622891404334723 1105 96-97 0.019178287900924573 1105 98-99 0.07436050205430078 1105 100-101 0.3418015156484451 1106 1 0.14806442541761555 1106 2 0.11854188926409392 1106 3 0.06934605554062045 1106 4 0.06128238602335756 1106 5 -0.0045623928522076085 1106 6 0.0059567570869276665 1106 7 -0.014366627641905438 1106 8 -0.08245377282234756 1106 9 0.010343899319281036 1106 10-11 -0.12373724779800455 1106 12-13 -0.35624971728744725 1106 14-15 -0.14585674999428022 1106 16-17 4.3744780239762804E-4 1106 18-19 -0.009943508804369117 1106 20-21 0.0681635131380176 1106 22-23 0.14521973827113044 1106 24-25 0.0766599346044643 1106 26-27 0.1404190577534692 1106 28-29 0.05121778265208121 1106 30-31 0.09660621834817817 1106 32-33 0.15861264796901509 1106 34-35 -0.3111257719187108 1106 36-37 0.13022343154981542 1106 38-39 0.1474624739240582 1106 40-41 -0.15499497691893538 1106 42-43 -0.23606179628102097 1106 44-45 -0.05514364323487797 1106 46-47 -0.27072141067105804 1106 48-49 -0.16621465807840963 1106 50-51 0.11818006826114669 1106 52-53 0.3014303605707198 1106 54-55 0.1844077189399016 1106 56-57 0.12904842169619002 1106 58-59 0.19312285398154216 1106 60-61 0.2480737056741802 1106 62-63 0.03527309993193484 1106 64-65 -0.02080867844899359 1106 66-67 -0.022501720678995696 1106 68-69 0.20726734150565562 1106 70-71 0.06728283999120777 1106 72-73 0.09216218108537788 1106 74-75 0.02520773260366127 1106 76-77 -0.003450920774675126 1106 78-79 -0.26044910378890407 1106 80-81 -0.25680637604357415 1106 82-83 -0.5698651004655595 1106 84-85 -0.7519781918809763 1106 86-87 -0.40625426665151565 1106 88-89 -0.5293833003818555 1106 90-91 -0.715128287088934 1106 92-93 -0.7313597100747344 1106 94-95 -0.6820795330382765 1106 96-97 -0.6560637641949683 1106 98-99 -1.0776735742842796 1106 100-101 -1.40378580719808 1107 1 0.15270007025527832 1107 2 0.08752617454018718 1107 3 0.017466372382720863 1107 4 0.01558016582563937 1107 5 -0.0024295274652601506 1107 6 -0.018713351920531807 1107 7 0.03741318890925527 1107 8 -0.025999587647234534 1107 9 0.005495273657025734 1107 10-11 0.010606776357484193 1107 12-13 0.02457935897700736 1107 14-15 -0.01157590675326503 1107 16-17 0.08277593649454218 1107 18-19 0.16830258900987616 1107 20-21 0.05736008628674227 1107 22-23 0.039628634959122166 1107 24-25 -0.030348153571637226 1107 26-27 -0.010017247222840808 1107 28-29 0.03579195857425077 1107 30-31 -0.05270862539625654 1107 32-33 0.07731970150668133 1107 34-35 0.11661258572399191 1107 36-37 0.10436307563745828 1107 38-39 0.01712636164953807 1107 40-41 -0.023307098788812652 1107 42-43 0.10235352447931234 1107 44-45 0.013094801536347234 1107 46-47 0.08980519206059512 1107 48-49 -0.07393995153847754 1107 50-51 0.10458547165599441 1107 52-53 0.18408404050232008 1107 54-55 0.17221940720526874 1107 56-57 0.056665003221475274 1107 58-59 -0.10431301993788367 1107 60-61 0.015667297498978883 1107 62-63 -0.06050254260268417 1107 64-65 -0.02573322311332049 1107 66-67 0.005862433731088856 1107 68-69 0.18349246529439256 1107 70-71 0.2696548757108417 1107 72-73 0.3073028758104819 1107 74-75 -0.10820641115737573 1107 76-77 -0.025934154903779927 1107 78-79 0.0830599234390128 1107 80-81 0.06447163118962251 1107 82-83 0.09797652740089191 1107 84-85 0.29997333409096427 1107 86-87 0.19245368364752125 1107 88-89 -0.012541510486929752 1107 90-91 -0.08926375308384138 1107 92-93 -0.06535695253353069 1107 94-95 -0.1569416814543203 1107 96-97 0.01742847932873204 1107 98-99 0.054973736603525225 1107 100-101 -0.03020215515779867 1108 1 0.09718073721653298 1108 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0.3215948049763391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 11.0 5 39.0 6 74.0 7 180.0 8 410.0 9 863.0 10 1439.0 11 2299.0 12 3493.0 13 4978.0 14 6446.0 15 8835.0 16 11746.0 17 16258.0 18 14778.0 19 9530.0 20 8322.0 21 8561.0 22 8987.0 23 9940.0 24 11574.0 25 13763.0 26 16731.0 27 20894.0 28 25678.0 29 31641.0 30 38595.0 31 48710.0 32 61342.0 33 79022.0 34 107580.0 35 151478.0 36 225467.0 37 323419.0 38 229459.0 39 16419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.329062080227565 20.028248215555614 15.674664840519398 21.968024863697423 2 15.567370563776029 20.784714925620747 40.30153287881541 23.346381631787814 3 19.242092678469387 24.98196148447766 30.508198966142892 25.267746870910063 4 12.995631255875683 17.670371804088557 37.304440108178426 32.029556831857334 5 13.366939148623407 37.62108235839588 34.47162385371855 14.540354639262166 6 31.053567807701526 36.993966972399754 17.440216146183076 14.512249073715644 7 27.604129635042646 33.430438480761985 20.800383417205847 18.165048466989518 8 26.008313517299857 35.85734299249756 19.456777346784534 18.677566143418048 9 25.881122232667884 17.115720368775726 20.700579075848665 36.302578322707724 10-11 24.680935527448828 27.397772967506974 27.052012808662703 20.8692786963815 12-13 25.558633425669434 24.68339908783122 28.094008357980982 21.66395912851836 14-15 22.509697347943213 26.03372544062695 26.66533681990188 24.791240391527953 16-17 21.454608774881958 29.350903110788085 27.353170433688046 21.841317680641907 18-19 21.273927366666403 27.512488709469302 30.31040071838059 20.903183205483707 20-21 22.061631155808005 26.504241026167698 30.698492138103926 20.735635679920367 22-23 21.72805166675993 26.413775169276448 30.564068296504527 21.294104867459094 24-25 21.586674386812902 26.662648591693248 30.704731069320353 21.045945952173497 26-27 21.47754530489148 26.81146324932511 30.57845227502924 21.13253917075417 28-29 21.196694972245787 26.910445545418128 30.743565410114893 21.1492940722212 30-31 21.432060630479036 26.699702166991884 30.717452329601862 21.15078487292722 32-33 21.29403965313383 26.814088738962933 30.559582401782258 21.332289206120983 34-35 21.71020927311366 26.865603842598812 30.316490363219334 21.107696521068195 36-37 21.662611292374052 26.75816275744385 30.356155347169008 21.223070603013092 38-39 21.51892859117877 26.95546725021923 30.424853124349887 21.100751034252113 40-41 21.622463234761632 26.881153050570063 30.30217345792719 21.194210256741115 42-43 21.560599960297615 26.8894104261883 30.112644814114827 21.437344799399263 44-45 21.707674664180775 27.03864850701659 29.795414267017428 21.458262561785208 46-47 21.771006970503738 27.26755331895519 29.432866438617694 21.528573271923378 48-49 21.915710098671997 27.25570319926898 29.29484546127249 21.533741240786533 50-51 21.94326162694243 27.49570103991384 28.994214508233203 21.56682282491053 52-53 22.058353977791544 27.7836772393943 28.678888537042514 21.479080245771645 54-55 21.901558918727464 27.635841272652286 28.780992640076054 21.6816071685442 56-57 21.9434591289372 27.907723118003492 28.65487316421896 21.493944588840346 58-59 21.989872097708187 27.620357715612926 28.77590897001393 21.613861216664958 60-61 21.99349296761231 27.52493133513982 29.062978119412342 21.418597577835534 62-63 21.878020134670027 27.647942160882494 29.01245052575031 21.461587178697172 64-65 21.880060988615984 27.562917552133943 28.868241152568974 21.688780306681096 66-67 21.822127070150074 27.769767974656546 28.72360385839897 21.684501096794413 68-69 21.7963859768317 27.667132771374327 28.747238263773127 21.789242988020845 70-71 21.891878191303064 27.695112220633426 28.610369381397422 21.802640206666087 72-73 22.000570122424904 27.586650708505488 28.55493715486526 21.857842014204344 74-75 21.869659216891424 27.906636857032257 28.29788830867192 21.9258156174044 76-77 21.91067379780536 27.751301538145533 28.415566580555797 21.92245808349331 78-79 21.915973434665023 27.76726628272279 28.406152318805088 21.910607963807102 80-81 21.813733235372343 28.02270219595885 28.31089052332762 21.852674045341193 82-83 21.89570183595711 27.928833002789734 28.30902672054116 21.866438440712 84-85 21.949647524773333 27.764270835802108 28.322279805026028 21.963801834398527 86-87 21.878053051669156 27.774639690527543 28.328830287852576 22.01847696995073 88-89 21.96334099641073 27.705843162349275 28.275768085257663 22.055047755982336 90-91 21.883904101133307 27.754244751158407 28.41990732739109 21.941943820317196 92-93 21.907556444474583 27.556233802528524 28.499221665514007 22.036988087482886 94-95 21.75764266885762 27.671148645267984 28.38307750241611 22.18813118345829 96-97 21.832463007876378 27.688364235812113 28.266320906507826 22.212851849803684 98-99 21.800554262816956 27.65882717355918 28.1234888865993 22.41712967702456 100-101 21.86232258616773 27.432367921142824 28.420288471352052 22.285021021337396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 800.0 1 562.5 2 408.5 3 537.0 4 441.0 5 361.5 6 646.5 7 1442.5 8 2371.0 9 2831.5 10 2561.0 11 2122.5 12 2075.0 13 2173.5 14 2390.5 15 2593.5 16 2743.0 17 2946.5 18 3102.0 19 3260.5 20 3618.0 21 4141.5 22 4836.0 23 5741.5 24 6901.0 25 8440.0 26 10369.5 27 12787.5 28 14807.0 29 17011.5 30 20118.5 31 22634.5 32 25047.5 33 28103.0 34 31726.5 35 34985.0 36 38688.0 37 42450.5 38 44657.5 39 46092.5 40 47711.0 41 49816.0 42 51735.5 43 53803.0 44 55965.5 45 57455.5 46 57843.0 47 57937.5 48 59948.5 49 59814.0 50 56581.0 51 53165.0 52 49951.0 53 47618.0 54 45087.5 55 41516.5 56 36727.0 57 32335.0 58 28687.5 59 25036.5 60 21057.5 61 17012.5 62 13346.5 63 10097.0 64 7521.5 65 5582.0 66 4191.0 67 3431.0 68 2566.0 69 1665.0 70 1150.5 71 858.5 72 698.5 73 529.0 74 422.0 75 357.0 76 248.5 77 142.5 78 78.0 79 57.5 80 33.0 81 18.0 82 13.5 83 9.5 84 7.5 85 5.5 86 1.5 87 1.5 88 2.5 89 1.5 90 1.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019223527490960995 2 0.0 3 0.0 4 0.0 5 0.03844705498192199 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004246292887558165 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.6083798779701006E-4 24-25 1.6458499564178932E-4 26-27 8.887589764656623E-4 28-29 8.887589764656623E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.583399825671573E-4 42-43 0.011290530701026747 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.583399825671572E-5 54-55 1.6458499564178932E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.254229834387994E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012146372678364051 92-93 0.0015470989590328195 94-95 0.0 96-97 0.0 98-99 0.001152094969492525 100-101 0.018236017517110258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1518972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.73157330080159 #Duplication Level Percentage of deduplicated Percentage of total 1 81.52292884234433 52.771074440542435 2 11.471334822945645 14.851151018990876 3 3.141268732051388 6.100178015589015 4 1.266612215194091 3.2795920580610787 5 0.6875668393444975 2.2253641630114402 6 0.41353011319679817 1.606107290069239 7 0.27396116764930634 1.2413756183684985 8 0.19448141478052358 1.0071270365207248 9 0.14661939573846025 0.8541813746307039 >10 0.8050024389262223 9.450054234020234 >50 0.05457691335360366 2.399129717973434 >100 0.020493937407881385 2.180441104573921 >500 0.0011159273587631946 0.43558147374156236 >1k 3.043438251172349E-4 0.5411113136786483 >5k 2.028958834114899E-4 1.0575311402282708 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8288 0.5456321775516599 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7816 0.5145585303744901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4999 0.3291041572853219 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1948 0.12824462860408223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.583399825671572E-5 0.0 0.0 0.0 0.0 6 6.583399825671572E-5 0.0 0.0 0.0 0.0 7 6.583399825671572E-5 0.0 0.0 0.0 0.0 8 6.583399825671572E-5 0.0 0.0 0.0 0.0 9 6.583399825671572E-5 0.0 0.0 0.0 0.0 10-11 1.3166799651343145E-4 0.0 0.0 6.583399825671572E-5 0.0 12-13 1.3166799651343145E-4 0.0 0.0 6.583399825671572E-5 0.0 14-15 1.3166799651343145E-4 0.0 0.0 6.583399825671572E-5 0.0 16-17 1.3166799651343145E-4 0.0 0.0 6.583399825671572E-5 0.0 18-19 1.3166799651343145E-4 0.0 0.0 1.3166799651343145E-4 0.0 20-21 1.3166799651343145E-4 0.0 0.0 1.9750199477014716E-4 0.0 22-23 1.3166799651343145E-4 0.0 0.0 3.6208699041193645E-4 0.0 24-25 1.975019947701472E-4 0.0 0.0 4.6083798779701006E-4 0.0 26-27 1.975019947701472E-4 0.0 0.0 8.229249782089466E-4 0.0 28-29 1.975019947701472E-4 0.0 0.0 0.0015800159581611773 0.0 30-31 1.975019947701472E-4 0.0 0.0 0.002633359930268629 0.0 32-33 1.975019947701472E-4 0.0 0.0 0.004410877883199954 0.0 34-35 1.975019947701472E-4 0.0 0.0 0.006320063832644709 0.0 36-37 1.975019947701472E-4 0.0 0.0 0.008558419773373044 0.0 38-39 1.975019947701472E-4 0.0 0.0 0.012673044664417777 0.0 40-41 1.975019947701472E-4 0.0 0.0 0.018565187508393834 0.0 42-43 2.3041899389850503E-4 0.0 0.0 0.025379006327963916 0.0 44-45 2.633359930268629E-4 0.0 0.0 0.03380575810482353 0.0 46-47 2.633359930268629E-4 0.0 0.0 0.04921091369689501 0.0 48-49 3.2916999128357864E-4 0.0 0.0 0.06076478039094861 0.0 50-51 3.2916999128357864E-4 0.0 0.0 0.07462283702398728 0.0 52-53 3.2916999128357864E-4 0.0 0.0 0.09091675159252442 0.0 54-55 3.2916999128357864E-4 0.0 0.0 0.10925152010701975 0.0 56-57 3.2916999128357864E-4 0.0 0.0 0.1442093731813358 0.0 58-59 3.2916999128357864E-4 0.0 0.0 0.18183350318504884 0.0 60-61 3.2916999128357864E-4 0.0 0.0 0.21359840734391417 0.0 62-63 3.2916999128357864E-4 0.0 0.0 0.2509921183537287 0.0 64-65 3.2916999128357864E-4 0.0 0.0 0.29108502329206853 0.0 66-67 3.2916999128357864E-4 0.0 0.0 0.34813018278151275 0.0 68-69 3.2916999128357864E-4 0.0 0.0 0.4217984268307777 0.0 70-71 3.2916999128357864E-4 0.0 0.0 0.472326020492807 0.0 72-73 3.2916999128357864E-4 0.0 0.0 0.5260794800694153 0.0 74-75 3.2916999128357864E-4 0.0 0.0 0.6102153298414981 0.0 76-77 3.2916999128357864E-4 0.0 0.0 0.7096905012073955 0.0 78-79 3.2916999128357864E-4 0.0 0.0 0.818184930334463 0.0 80-81 3.2916999128357864E-4 0.0 0.0 0.9227951535643844 0.0 82-83 3.2916999128357864E-4 0.0 0.0 1.0535414741022218 0.0 84-85 3.2916999128357864E-4 0.0 0.0 1.2152956078189723 0.0 86-87 3.2916999128357864E-4 0.0 0.0 1.3862994182907915 0.0 88-89 3.2916999128357864E-4 3.291699912835786E-5 0.0 1.601872845582407 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4595 0.0 46.8472 1 GTATCAA 7405 0.0 37.155613 1 ATCAACG 9415 0.0 29.067091 3 TATCAAC 9465 0.0 28.913542 2 TCAACGC 9505 0.0 28.791864 4 CAACGCA 9625 0.0 28.383537 5 AACGCAG 9875 0.0 27.656773 6 ACGCAGA 11235 0.0 24.351988 7 CGCAGAG 11270 0.0 24.234215 8 GTACATG 5840 0.0 23.352854 1 GAGTACT 7315 0.0 22.856691 12-13 GCAGAGT 11930 0.0 22.694439 9 GTACCTG 1210 0.0 22.385172 1 TACATGG 6080 0.0 21.802185 2 GTACTTT 7655 0.0 21.592592 14-15 TACCTGG 1255 0.0 21.200384 2 AGTACTT 7560 0.0 21.07928 12-13 ACATGGG 6005 0.0 20.966806 3 CAGAGTA 11740 0.0 19.986828 10-11 AGAGTAC 11075 0.0 19.707285 10-11 >>END_MODULE