FastQCFastQC Report
Thu 26 May 2016
SRR937874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413004
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66020.46723151526818046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56420.3992911555805928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.2538563231243507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19360.1370130587033016No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.10955382999623497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.048.5168271
GTATCAA66050.038.4422651
ATCAACG82850.030.519313
TATCAAC84350.030.0339952
TCAACGC84200.029.9767284
CAACGCA84950.029.7110185
AACGCAG87950.028.6843326
ACGCAGA99650.025.078097
CGCAGAG100800.024.603458
GCAGAGT103800.023.2973489
GTACATG59150.022.5083581
GAGTACT61900.022.41068512-13
TACATGG59200.021.8382662
ACATGGG59400.021.0438353
GTGGTAT21050.021.0073551
GTACTTT66300.020.81554614-15
GTATAGA5750.020.6734371
CAGAGTA101650.020.54102710-11
AGTACTT65150.020.52706712-13
AGAGTAC96550.020.44510810-11
Produced by FastQC (version 0.11.5)