FastQCFastQC Report
Thu 26 May 2016
SRR937874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413004
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79180.5603664250065817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72470.5128789444332783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.3263260401244441No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.13149290447868514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.046.254261
GTATCAA71650.037.0038071
ATCAACG86800.030.2672023
TATCAAC88050.029.8914722
TCAACGC88150.029.7497734
CAACGCA89200.029.3995825
AACGCAG92300.028.4565836
ACGCAGA105550.024.7069747
CGCAGAG105900.024.5356068
GTACCTG11850.023.657131
GAGTACT66100.023.64285312-13
GCAGAGT111650.022.8891129
TACCTGG11650.022.836472
TAGGACG1252.2536819E-422.8038464
GTACATG57050.022.5705431
TACATGG58000.021.951982
GTACTTT71100.021.84426514-15
ACATGGG58250.020.879063
TAGACCG1150.00372005620.6556594
CAGAGTA109550.020.40105610-11
Produced by FastQC (version 0.11.5)