##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937874_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1413004 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11239741713399 33.0 31.0 34.0 30.0 34.0 2 32.333406699485636 34.0 31.0 34.0 31.0 34.0 3 32.40567967252747 34.0 31.0 34.0 31.0 34.0 4 36.05094606950865 37.0 35.0 37.0 35.0 37.0 5 35.9517842836963 37.0 35.0 37.0 35.0 37.0 6 35.98396253655333 37.0 35.0 37.0 35.0 37.0 7 35.92629744855641 37.0 35.0 37.0 35.0 37.0 8 35.910761752974516 37.0 35.0 37.0 35.0 37.0 9 37.57219017072846 39.0 37.0 39.0 35.0 39.0 10-11 37.49670453869911 39.0 37.0 39.0 35.0 39.0 12-13 37.38347343673479 39.0 37.0 39.0 34.0 39.0 14-15 38.569920184231606 40.0 38.0 41.0 34.0 41.0 16-17 38.52179965520267 40.0 38.0 41.0 34.0 41.0 18-19 38.516997828739335 40.0 38.0 41.0 34.0 41.0 20-21 38.41688664717155 40.0 38.0 41.0 34.0 41.0 22-23 38.25251379330844 40.0 38.0 41.0 34.0 41.0 24-25 38.32307976481312 40.0 38.0 41.0 34.0 41.0 26-27 38.18829883001039 40.0 38.0 41.0 33.5 41.0 28-29 38.03500697804111 40.0 37.0 41.0 33.0 41.0 30-31 38.07347219116153 40.0 37.0 41.0 33.5 41.0 32-33 37.98693138872926 40.0 37.0 41.0 33.0 41.0 34-35 37.84824671409281 40.0 37.0 41.0 33.0 41.0 36-37 37.674028523627676 40.0 37.0 41.0 33.0 41.0 38-39 37.521162006618525 40.0 37.0 41.0 33.0 41.0 40-41 37.33978000062279 40.0 36.0 41.0 32.0 41.0 42-43 37.11482663884887 40.0 36.0 41.0 31.5 41.0 44-45 36.79189655514068 39.0 36.0 41.0 31.0 41.0 46-47 36.4228462905979 39.0 36.0 41.0 30.0 41.0 48-49 36.076579754905154 39.0 35.0 41.0 29.5 41.0 50-51 35.82495095555285 39.0 35.0 40.0 28.5 41.0 52-53 35.56139048438646 39.0 35.0 40.0 28.0 41.0 54-55 35.32927472250609 38.5 35.0 40.0 27.0 41.0 56-57 35.05506247682243 38.0 34.0 40.0 27.0 41.0 58-59 34.781935861469606 38.0 34.0 40.0 26.0 41.0 60-61 34.41727978123204 38.0 34.0 40.0 26.0 41.0 62-63 34.05549064263088 37.0 33.0 40.0 25.0 41.0 64-65 33.659247602979185 37.0 33.0 39.5 23.5 41.0 66-67 33.20571137802865 36.0 32.5 39.0 22.5 40.0 68-69 32.75206156528927 35.5 32.0 39.0 22.0 40.0 70-71 32.324673532417464 35.0 31.0 38.0 20.5 40.0 72-73 31.806781863320982 35.0 31.0 37.5 19.5 39.5 74-75 31.27474586059204 35.0 30.5 37.0 18.0 39.0 76-77 30.052936509733872 33.5 29.5 35.5 17.5 38.0 78-79 30.668120189327134 34.0 30.0 36.0 17.5 38.5 80-81 30.465990542135764 34.0 30.0 36.0 14.0 37.5 82-83 30.078115490118925 34.0 30.0 35.0 9.0 37.0 84-85 29.651584142720047 34.0 29.5 35.0 7.0 36.5 86-87 29.18007450792779 34.0 29.0 35.0 2.0 36.0 88-89 28.756412579157598 34.0 29.0 35.0 2.0 36.0 90-91 28.173489247022655 33.0 27.5 35.0 2.0 35.0 92-93 27.636864085310446 33.0 26.5 35.0 2.0 35.0 94-95 27.09734650432695 33.0 26.0 35.0 2.0 35.0 96-97 26.4657778746557 32.5 25.0 34.0 2.0 35.0 98-99 25.484910870740634 32.0 22.0 34.0 2.0 35.0 100-101 22.788180005152142 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29752129102946157 1101 2 0.1911438081486594 1101 3 0.15353321811405607 1101 4 0.10619387696765159 1101 5 0.10843698274343438 1101 6 0.1749621845005649 1101 7 0.10546521722212532 1101 8 0.05719458236652031 1101 9 0.16136503439119565 1101 10-11 0.19090816200937866 1101 12-13 0.2658877426680135 1101 14-15 0.20196952174610772 1101 16-17 0.25430654067920955 1101 18-19 0.2639482362035537 1101 20-21 0.3592542076269254 1101 22-23 0.3552913471103807 1101 24-25 0.30229493295493626 1101 26-27 0.24014441766394867 1101 28-29 0.25236826023407133 1101 30-31 0.35077125672339804 1101 32-33 0.37880665384403756 1101 34-35 0.3049774443810733 1101 36-37 0.37423631231055765 1101 38-39 0.35307978938783435 1101 40-41 0.35070437473063265 1101 42-43 0.28090848797617696 1101 44-45 0.3514372183784715 1101 46-47 0.2853314087333132 1101 48-49 0.46304827495065837 1101 50-51 0.469728802832023 1101 52-53 0.4406158516386043 1101 54-55 0.5655995820841113 1101 56-57 0.3562597220175263 1101 58-59 0.3548028942379631 1101 60-61 0.28328367614620475 1101 62-63 0.232326787148466 1101 64-65 0.1707205117198427 1101 66-67 0.25702561918420486 1101 68-69 0.3093571077547921 1101 70-71 0.4237816213063681 1101 72-73 0.2684720714420301 1101 74-75 0.15268741154184795 1101 76-77 0.1935333974589497 1101 78-79 0.27459878231691803 1101 80-81 0.3475341912254528 1101 82-83 0.23507102748836317 1101 84-85 0.1092658889478102 1101 86-87 0.09833437987902016 1101 88-89 0.3543987833187039 1101 90-91 0.37821659783860184 1101 92-93 0.47113435439699103 1101 94-95 0.36580909411405926 1101 96-97 0.5100790096361365 1101 98-99 0.5583081133274561 1101 100-101 1.1538715985853933 1102 1 0.27978047846540477 1102 2 0.24900575532632274 1102 3 0.21403897156000085 1102 4 0.10818300652551471 1102 5 0.11669887733181383 1102 6 0.22559131358033824 1102 7 0.17633075899623663 1102 8 0.08883778804138132 1102 9 0.20413146928657255 1102 10-11 0.2018456853533408 1102 12-13 0.17718248463694408 1102 14-15 0.208908317989561 1102 16-17 0.20111383385545167 1102 18-19 0.24902065103731985 1102 20-21 0.18855270853379835 1102 22-23 0.29955567992877974 1102 24-25 0.20633369961692694 1102 26-27 0.25962336551988585 1102 28-29 0.3385147213643265 1102 30-31 0.2487900454370191 1102 32-33 0.24085975705946083 1102 34-35 0.29562073670872024 1102 36-37 0.35887396921931725 1102 38-39 0.37717987036336353 1102 40-41 0.4813582446737712 1102 42-43 0.5176645066095134 1102 44-45 0.4909411760201934 1102 46-47 0.5469507998331835 1102 48-49 0.49815579910138297 1102 50-51 0.512403355897888 1102 52-53 0.5577164794230711 1102 54-55 0.5458130168509996 1102 56-57 0.41223771818687993 1102 58-59 0.445844042357443 1102 60-61 0.21181993137151522 1102 62-63 0.4153382103755945 1102 64-65 0.5050674336038554 1102 66-67 0.5122274753465632 1102 68-69 0.3791772882913875 1102 70-71 0.33599996241355967 1102 72-73 0.41846026873548325 1102 74-75 0.30981998133468736 1102 76-77 0.39497258065189556 1102 78-79 0.5359804758708471 1102 80-81 0.48262498242511853 1102 82-83 0.49615496415414384 1102 84-85 0.48378115627164675 1102 86-87 0.598967542795215 1102 88-89 0.7154111567384085 1102 90-91 0.5789172901644513 1102 92-93 0.5906778436356106 1102 94-95 0.8554914369662505 1102 96-97 0.8114664999623997 1102 98-99 0.7406883158559232 1102 100-101 1.1358273518819324 1103 1 0.17354245107431865 1103 2 0.19738437742667259 1103 3 0.09765989656239782 1103 4 0.05846517645509408 1103 5 -0.06169177413328697 1103 6 0.035246588655070354 1103 7 0.11248431645240231 1103 8 0.05867414155182615 1103 9 0.11696886480552848 1103 10-11 0.07666241427422449 1103 12-13 0.07477008835644483 1103 14-15 0.13806107827759462 1103 16-17 0.11981461073516186 1103 18-19 0.009393894695456595 1103 20-21 0.04910748467438708 1103 22-23 0.2123245373805176 1103 24-25 0.05747045447019872 1103 26-27 -0.07225246821624154 1103 28-29 -0.08408496632049633 1103 30-31 -0.04197860970528211 1103 32-33 -0.037261828609679526 1103 34-35 -0.035769439444173656 1103 36-37 0.0011381623466633073 1103 38-39 -0.15217381997331358 1103 40-41 -0.2074009088221942 1103 42-43 -0.03323010914338198 1103 44-45 -0.1001638760256256 1103 46-47 0.06721737699324848 1103 48-49 -0.09415635630444541 1103 50-51 -0.09862213451569346 1103 52-53 -0.21357946191638888 1103 54-55 -0.1724879115308866 1103 56-57 -0.3239667327355562 1103 58-59 -0.3439814600107809 1103 60-61 -0.46419344059219014 1103 62-63 -0.2980085796002001 1103 64-65 -0.3017523483502558 1103 66-67 -0.18451212425318175 1103 68-69 -0.13667655808794166 1103 70-71 -0.297357874116976 1103 72-73 -0.34520093423548204 1103 74-75 -0.08609641429289994 1103 76-77 0.017822827417674603 1103 78-79 0.08529828502063452 1103 80-81 0.016739984991342993 1103 82-83 0.04508740832219971 1103 84-85 0.19861394350362005 1103 86-87 0.2843924701908449 1103 88-89 0.16778409693241159 1103 90-91 0.059521017140976795 1103 92-93 0.1380133652059925 1103 94-95 0.11634631500872317 1103 96-97 0.13005658139768883 1103 98-99 0.34457492716720495 1103 100-101 0.8494396729860831 1104 1 0.10113686890412765 1104 2 0.05035161709081848 1104 3 -0.06536428069447453 1104 4 -0.002048699183063718 1104 5 -0.006049452220295848 1104 6 -0.04249908557045501 1104 7 0.038004829716904 1104 8 -0.01009517575161567 1104 9 0.02415017669022035 1104 10-11 0.03791802909497477 1104 12-13 0.07040442021965987 1104 14-15 0.06872532755809146 1104 16-17 -0.060417598672046324 1104 18-19 -0.04994099590536649 1104 20-21 -0.10142601525035388 1104 22-23 -0.04870167473606557 1104 24-25 -0.11509396672086325 1104 26-27 -0.22057993350309602 1104 28-29 -0.17124978882481656 1104 30-31 -0.06510080653892203 1104 32-33 -0.023296500446114976 1104 34-35 -0.09264874781822385 1104 36-37 -0.06536853356404038 1104 38-39 -0.06894375746758641 1104 40-41 -0.014948223837258467 1104 42-43 -0.0599846097709289 1104 44-45 0.2138196164862478 1104 46-47 0.05434275852944381 1104 48-49 -0.1115791351894373 1104 50-51 -0.11099596019906244 1104 52-53 -0.17645091152191839 1104 54-55 -0.2481316707474619 1104 56-57 -0.06873924832238032 1104 58-59 -0.0779094069640962 1104 60-61 -0.09188739452378059 1104 62-63 -0.18008149955596764 1104 64-65 -0.29463260285484694 1104 66-67 -0.2478276101240411 1104 68-69 -0.14814499569368422 1104 70-71 0.059441986453258266 1104 72-73 0.10704578627914074 1104 74-75 0.07270723985338279 1104 76-77 0.029656815459762953 1104 78-79 -0.0323761351631795 1104 80-81 -0.0035316540666485707 1104 82-83 0.08744542366626362 1104 84-85 0.010109033470904194 1104 86-87 0.13447633176153317 1104 88-89 0.06089652689215441 1104 90-91 0.06355423809301541 1104 92-93 0.023439616254261608 1104 94-95 0.10948403924788508 1104 96-97 0.21267522401725003 1104 98-99 0.3012770058250567 1104 100-101 0.38510177762857367 1105 1 0.054302226204612225 1105 2 0.09645709872758346 1105 3 0.05033023870999642 1105 4 0.04360268373553566 1105 5 -0.09545772977468658 1105 6 0.020001962905887183 1105 7 0.01976683686794445 1105 8 -2.747848102160333E-4 1105 9 0.04121474374324663 1105 10-11 0.10460372741492563 1105 12-13 0.09213188694462815 1105 14-15 0.07862135341337506 1105 16-17 -0.029088113319190256 1105 18-19 -0.12954797201862078 1105 20-21 0.04255147880731869 1105 22-23 0.11611374425822163 1105 24-25 0.16127065777420313 1105 26-27 -0.043181063023048694 1105 28-29 0.06691761336607982 1105 30-31 0.07197265159346955 1105 32-33 0.16545440413589318 1105 34-35 -0.04331873143017617 1105 36-37 -0.034084891935279416 1105 38-39 -0.035993978471928756 1105 40-41 0.025824490209544138 1105 42-43 0.16129147704349123 1105 44-45 0.059681446683406136 1105 46-47 0.021139175950864342 1105 48-49 0.17865473432745915 1105 50-51 0.24375159851416583 1105 52-53 0.04176058741456501 1105 54-55 0.13197730137550678 1105 56-57 0.09258756450044103 1105 58-59 0.3218273314733082 1105 60-61 0.285578549518533 1105 62-63 0.18890740481661794 1105 64-65 0.2202745614346 1105 66-67 0.12182060509445591 1105 68-69 0.06307265989586597 1105 70-71 0.05175614872211298 1105 72-73 0.01854064455116955 1105 74-75 -0.003523931680817327 1105 76-77 -0.0662084855556273 1105 78-79 -0.04217251961183166 1105 80-81 0.08316099745915295 1105 82-83 0.19968850357780354 1105 84-85 0.18707115932392782 1105 86-87 0.10925085873239126 1105 88-89 0.15126737803512214 1105 90-91 0.20184175951808925 1105 92-93 0.2915373301998372 1105 94-95 0.41287245446649834 1105 96-97 0.46723342067265605 1105 98-99 0.6779734405427256 1105 100-101 0.43633683937153833 1106 1 -0.05402071087218019 1106 2 0.01228422303451282 1106 3 0.07261689339495803 1106 4 0.03090760446465879 1106 5 -0.008946589242455616 1106 6 0.01797271301093417 1106 7 -0.0966408007392019 1106 8 -0.017338931654123257 1106 9 -0.12298182206031782 1106 10-11 -0.2155531465836873 1106 12-13 -0.352392632226568 1106 14-15 -0.13713443347376852 1106 16-17 -1.3844450094779859E-5 1106 18-19 -0.026457015100270098 1106 20-21 -0.03020275120353233 1106 22-23 -0.16840174981319223 1106 24-25 -0.07983739838514481 1106 26-27 -0.04242747029729088 1106 28-29 -0.07093550608543353 1106 30-31 0.068967131872256 1106 32-33 0.01129104113400814 1106 34-35 -0.21258081306043408 1106 36-37 -0.30248440260467646 1106 38-39 -0.1993857078655239 1106 40-41 -0.20358773852114354 1106 42-43 -0.2167747975002925 1106 44-45 -0.13019348456464996 1106 46-47 0.08097596015812769 1106 48-49 0.0211585131609624 1106 50-51 0.3017981147355897 1106 52-53 0.12850838059754466 1106 54-55 0.24012725219710518 1106 56-57 0.08865329182379611 1106 58-59 0.09796836527270614 1106 60-61 0.1279835677969956 1106 62-63 0.15119360607262422 1106 64-65 -0.20650830727380054 1106 66-67 -0.2639235261837811 1106 68-69 -0.03211931602643858 1106 70-71 0.12821793692793193 1106 72-73 0.02843719752829088 1106 74-75 -0.03413473465692718 1106 76-77 -0.1462963079133388 1106 78-79 -0.13683016366326584 1106 80-81 -0.33565114909529825 1106 82-83 -0.26095448353767026 1106 84-85 -0.18933994421968237 1106 86-87 -0.30503771277692593 1106 88-89 -0.47618901902454525 1106 90-91 -0.4040447733000754 1106 92-93 -0.4318899467822135 1106 94-95 -0.14637782248232512 1106 96-97 -0.20192042808518096 1106 98-99 -0.3171882239639423 1106 100-101 -0.2212483594648269 1107 1 0.11264766536179849 1107 2 0.14787000627277536 1107 3 0.023557630294455123 1107 4 -0.02694795229392355 1107 5 -0.016160583380539606 1107 6 0.06076090331996653 1107 7 0.04538739192523167 1107 8 -0.04433072003248384 1107 9 -0.023095603262298425 1107 10-11 0.06100507653003717 1107 12-13 0.09388649452645836 1107 14-15 0.139867137428638 1107 16-17 0.15926882110724705 1107 18-19 0.1991017759697158 1107 20-21 0.23347274252900974 1107 22-23 0.3325696643218876 1107 24-25 0.2579446089887867 1107 26-27 0.19789115505863464 1107 28-29 0.27077926590578016 1107 30-31 0.34520509604352867 1107 32-33 0.2406511500904358 1107 34-35 0.2431522209086694 1107 36-37 0.1497029887665633 1107 38-39 0.17646133524785768 1107 40-41 0.26679438776270104 1107 42-43 0.14785843382885844 1107 44-45 0.36249336639337315 1107 46-47 0.21882748993593992 1107 48-49 0.31698450960126223 1107 50-51 0.27268304376005403 1107 52-53 0.32474885213419213 1107 54-55 0.30164242270744523 1107 56-57 0.27837385354961697 1107 58-59 0.2338607309149694 1107 60-61 0.28055979075497106 1107 62-63 0.45192169179412645 1107 64-65 0.4997131880657264 1107 66-67 0.33264005357417403 1107 68-69 0.3929376220625329 1107 70-71 0.32464247187812845 1107 72-73 0.4986348804947305 1107 74-75 0.41397666834697233 1107 76-77 0.17804451142528777 1107 78-79 0.528787398531744 1107 80-81 0.5003874795277987 1107 82-83 0.3568364135862687 1107 84-85 0.46487518393138316 1107 86-87 0.4421955424861679 1107 88-89 0.46736201820793255 1107 90-91 0.6105251090626957 1107 92-93 0.7123652422294455 1107 94-95 0.957453887711825 1107 96-97 1.18188294128203 1107 98-99 0.9744440139166528 1107 100-101 1.1363141014763087 1108 1 0.03211021379880208 1108 2 -0.007225233839619705 1108 3 -0.08197886311852187 1108 4 -0.039984844095585004 1108 5 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0.010562087105348894 2316 50-51 0.025806150898318947 2316 52-53 0.06612229731080532 2316 54-55 -0.03688660576118963 2316 56-57 -0.03747876785355686 2316 58-59 -0.12116395659077028 2316 60-61 -0.1539170605020459 2316 62-63 -0.10975424415908464 2316 64-65 0.09954187451911167 2316 66-67 -0.1388307344069588 2316 68-69 0.030443663924216935 2316 70-71 0.025807403995138145 2316 72-73 0.2736286091952529 2316 74-75 0.2729452384295108 2316 76-77 0.24813744902432688 2316 78-79 0.1465140677681127 2316 80-81 -0.007161941371233382 2316 82-83 0.14372664443127192 2316 84-85 0.3064113769772767 2316 86-87 0.37420914967644237 2316 88-89 0.6113969416980787 2316 90-91 0.5598003915770917 2316 92-93 0.6635837098297728 2316 94-95 0.6466562396020876 2316 96-97 0.8808893227116421 2316 98-99 0.9367611520014876 2316 100-101 1.5250502357861286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 14.0 5 44.0 6 80.0 7 166.0 8 431.0 9 853.0 10 1343.0 11 2187.0 12 3337.0 13 4572.0 14 6037.0 15 8250.0 16 11071.0 17 15262.0 18 14053.0 19 9109.0 20 7744.0 21 8045.0 22 8533.0 23 9323.0 24 10882.0 25 12923.0 26 15800.0 27 19165.0 28 24111.0 29 29468.0 30 36732.0 31 45407.0 32 57747.0 33 74027.0 34 99671.0 35 140714.0 36 206141.0 37 295455.0 38 216773.0 39 17528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42442260484001 20.02868217712731 15.638086420277423 21.90880879775526 2 15.607599129231057 20.766254023343176 40.32515123807151 23.300995609354256 3 19.23526048050819 25.014720411265646 30.534025381386037 25.215993726840125 4 12.96206652512385 17.691861288039632 37.37218683651805 31.973885350318472 5 13.358231247637505 37.587757361425126 34.47443268290128 14.57957870803609 6 30.983917950692287 37.03627165952821 17.432010100466808 14.547800289312699 7 27.616553102468217 33.49431424114864 20.736883972019896 18.152248684363244 8 26.015071436457365 35.799474028382086 19.459109811437195 18.726344723723358 9 25.843946655494253 17.209576193697966 20.691731941310852 36.25474520949693 10-11 24.65990895991802 27.44418982536497 27.037255379319518 20.858645835397493 12-13 25.60994123261121 24.698333655945902 28.033994282149287 21.657730829293598 14-15 22.50241329819307 26.000775652439767 26.781594390390968 24.715216658976193 16-17 21.391057633240955 29.413434073788892 27.396737730395664 21.79877056257449 18-19 21.270463494795486 27.55476983787732 30.35745121740632 20.81731544992088 20-21 22.057227014219354 26.57674712881209 30.707237912985384 20.658787943983175 22-23 21.693949991560398 26.411881542681204 30.66788206356559 21.22628640219281 24-25 21.571594984869115 26.65608165298895 30.75047912107821 21.021844241063718 26-27 21.471145666577492 26.830439478157725 30.647263894963462 21.051150960301317 28-29 21.195915454132724 26.893076321785554 30.82818672108576 21.082821502995962 30-31 21.4048934044065 26.68265624159592 30.792658761050923 21.119791592946658 32-33 21.28359155388095 26.81974007150723 30.654584134227502 21.242084240384315 34-35 21.62669037030327 26.877277063617655 30.42110991900943 21.074922647069645 36-37 21.66840999742393 26.682903940823948 30.45072766956074 21.19795839219139 38-39 21.523407408403447 26.968262994394564 30.43626572757959 21.072063869622404 40-41 21.600567023332744 26.861668812230494 30.401424493964228 21.136339670472537 42-43 21.545270926214762 26.88968473313323 30.192485536244597 21.372558804407415 44-45 21.694107622521813 27.114334819411273 29.86146177274154 21.330095785325373 46-47 21.805458441731236 27.288068540499534 29.44779349527673 21.458679522492506 48-49 21.90114111495792 27.312626149678277 29.267609999688602 21.5186227356752 50-51 21.979166371786633 27.579327447056055 28.938240797619823 21.503265383537485 52-53 21.980971037590834 27.847904181446054 28.717894641487213 21.453230139475895 54-55 21.941020690670374 27.678159439039096 28.77840402433397 21.602415845956557 56-57 21.90209652626603 27.963332021706943 28.67415803493833 21.4604134170887 58-59 21.971169225281738 27.62455024897311 28.854766157774502 21.549514367970648 60-61 21.959704289584458 27.604592768314877 29.096874460369538 21.33882848173112 62-63 21.81975422574883 27.73626260081359 29.06212579723766 21.381857376199925 64-65 21.846894984019862 27.518994992229324 28.95476587468967 21.67934414906115 66-67 21.79809823609841 27.830954477128163 28.71106521991445 21.659882066858977 68-69 21.791913466597922 27.641757433721853 28.775795672126787 21.790533427553434 70-71 21.933235857789505 27.649037086943846 28.65986225092073 21.75786480434592 72-73 22.014622746998594 27.64606469620751 28.562374911889847 21.77693764490405 74-75 21.825451307993465 27.98746500363764 28.275999218685865 21.91108446968303 76-77 21.890950061004784 27.839765492525142 28.44779632612505 21.821488120345023 78-79 21.990949777919948 27.764464927204735 28.411703010041016 21.832882284834294 80-81 21.772600624344037 28.00822078934015 28.398807362758603 21.820371223557203 82-83 21.911954439327573 27.981178692020304 28.324638899681737 21.782227968970393 84-85 21.938923372367427 27.796939197612446 28.324400183864707 21.93973724615542 86-87 21.87481422557898 27.803459862817093 28.33247464267617 21.98925126892776 88-89 21.872443390110714 27.74404743369446 28.342559539817298 22.040949636377533 90-91 21.901708569887578 27.828299307979293 28.383076671534003 21.88691545059912 92-93 21.873027196729712 27.60728511129795 28.515376187928354 22.00431150404398 94-95 21.709068056424467 27.675293205114777 28.437923742607946 22.177714995852806 96-97 21.825592850409482 27.671683873506375 28.31064172500573 22.19208155107841 98-99 21.780823032714867 27.61540048471972 28.154402587719655 22.449373894845756 100-101 21.75985242422655 27.47979398286116 28.517206083678182 22.243147509234102 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 658.0 1 459.0 2 363.0 3 490.0 4 390.5 5 306.5 6 585.0 7 1365.0 8 2251.5 9 2694.5 10 2387.5 11 1921.0 12 1939.5 13 2136.5 14 2342.0 15 2505.0 16 2696.5 17 2869.5 18 2999.5 19 3133.5 20 3406.0 21 3867.5 22 4525.5 23 5389.5 24 6477.5 25 7954.5 26 9819.0 27 11946.0 28 13765.0 29 15946.5 30 18802.5 31 21102.0 32 23427.0 33 26385.5 34 29750.0 35 32735.0 36 36112.0 37 39572.0 38 41628.5 39 43086.5 40 44561.0 41 46405.5 42 48236.5 43 50277.0 44 52140.5 45 53540.0 46 53954.5 47 53920.5 48 55794.5 49 55346.0 50 52323.0 51 49429.5 52 46384.5 53 43914.5 54 41378.0 55 38150.0 56 33816.5 57 29572.5 58 26244.0 59 23071.0 60 19444.0 61 15723.0 62 12412.0 63 9425.5 64 6941.5 65 5220.0 66 3932.5 67 3163.0 68 2428.0 69 1604.0 70 1083.0 71 787.0 72 649.5 73 510.0 74 404.5 75 335.5 76 224.5 77 146.0 78 90.0 79 54.0 80 31.0 81 22.0 82 17.5 83 12.0 84 7.5 85 4.5 86 4.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019815938242213043 2 0.0 3 0.0 4 2.830848320316149E-4 5 0.021372904818386926 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004281658084478175 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001592352180177834 24-25 0.0 26-27 7.077120800790372E-4 28-29 0.0013800385561541229 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.538560400395186E-5 40-41 7.784832880869411E-4 42-43 0.017586645189964077 44-45 3.538560400395186E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.538560400395186E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.077120800790372E-5 82-83 0.0021939074482450155 84-85 6.015552680671818E-4 86-87 0.0 88-89 0.0 90-91 0.01309267348146219 92-93 0.002830848320316149 94-95 0.0 96-97 0.0 98-99 0.001592352180177834 100-101 0.02165598965041854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1413004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.45692448084542 #Duplication Level Percentage of deduplicated Percentage of total 1 82.01124513442471 53.6820387934414 2 11.183921868221292 14.641302582556733 3 3.0258055103234796 5.9417976834890975 4 1.2802974019571574 3.3521732141172893 5 0.66153827599586 2.165113048652484 6 0.39084223059191675 1.534999822306816 7 0.2643818113946859 1.211393418379975 8 0.1941098773950805 1.0164668468508757 9 0.14518855082273774 0.85532364060186 >10 0.769155353471267 9.221195077358022 >50 0.05237393833684946 2.292446040258486 >100 0.019414390323686095 2.0270527993904075 >500 0.0011863890095837899 0.4426470420898173 >1k 3.2356063897739717E-4 0.545437168610196 >5k 2.1570709265159813E-4 1.0706128218966633 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7918 0.5603664250065817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7247 0.5128789444332783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.3263260401244441 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.13149290447868514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.538560400395186E-5 0.0 12-13 0.0 0.0 0.0 7.077120800790372E-5 0.0 14-15 0.0 0.0 0.0 7.077120800790372E-5 0.0 16-17 0.0 0.0 0.0 7.077120800790372E-5 0.0 18-19 1.0615681201185559E-4 0.0 0.0 7.077120800790372E-5 0.0 20-21 1.4154241601580745E-4 0.0 0.0 1.4154241601580745E-4 0.0 22-23 1.4154241601580745E-4 0.0 0.0 3.892416440434705E-4 0.0 24-25 2.1231362402371117E-4 0.0 0.0 6.369408720711335E-4 0.0 26-27 2.1231362402371117E-4 0.0 0.0 8.492544960948447E-4 0.0 28-29 2.1231362402371117E-4 0.0 0.0 0.0017338945961936413 0.0 30-31 2.830848320316149E-4 0.0 0.0 0.003821645232426801 0.0 32-33 2.830848320316149E-4 0.0 0.0 0.00587401026465601 0.0 34-35 2.830848320316149E-4 0.0 0.0 0.007430976840829891 0.0 36-37 3.538560400395186E-4 0.0 0.0 0.011040308449232983 0.0 38-39 3.538560400395186E-4 0.0 0.0 0.015640436969746725 0.0 40-41 3.538560400395186E-4 0.0 0.0 0.024416066762726785 0.0 42-43 4.2462724804742235E-4 0.0 0.0 0.03198858601957249 0.0 44-45 4.953984560553261E-4 0.0 0.0 0.040056503732473506 0.0 46-47 4.953984560553261E-4 0.0 0.0 0.05697082244636251 0.0 48-49 4.953984560553261E-4 0.0 0.0 0.06889577099569427 0.0 50-51 4.953984560553261E-4 0.0 0.0 0.08471313598546076 0.0 52-53 4.953984560553261E-4 0.0 0.0 0.10091974261927071 0.0 54-55 4.953984560553261E-4 0.0 0.0 0.12094799448550747 0.0 56-57 4.953984560553261E-4 0.0 0.0 0.1626676216061667 0.0 58-59 5.661696640632298E-4 0.0 0.0 0.20396262147877853 0.0 60-61 6.015552680671817E-4 0.0 0.0 0.23910052625470274 0.0 62-63 6.369408720711335E-4 0.0 0.0 0.28234173434753196 0.0 64-65 6.369408720711335E-4 0.0 0.0 0.32448598871623857 0.0 66-67 7.077120800790372E-4 0.0 0.0 0.3882508471313598 0.0 68-69 7.077120800790372E-4 0.0 0.0 0.4698854355684768 0.0 70-71 7.077120800790372E-4 0.0 0.0 0.5259716179147405 0.0 72-73 7.430976840829891E-4 0.0 0.0 0.5828716691530951 0.0 74-75 8.846401000987966E-4 0.0 0.0 0.6738834426512593 0.0 76-77 9.200257041027484E-4 0.0 0.0 0.7808895091592097 0.0 78-79 9.200257041027484E-4 0.0 0.0 0.895432709320002 0.0 80-81 9.200257041027484E-4 0.0 0.0 1.009338968608723 0.0 82-83 9.200257041027484E-4 0.0 0.0 1.1446889039238388 0.0 84-85 9.200257041027484E-4 0.0 0.0 1.3059057157658436 0.0 86-87 9.907969121106521E-4 0.0 0.0 1.4793659465932154 0.0 88-89 9.907969121106521E-4 0.0 0.0 1.7109293391950766 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4335 0.0 46.25426 1 GTATCAA 7165 0.0 37.003807 1 ATCAACG 8680 0.0 30.267202 3 TATCAAC 8805 0.0 29.891472 2 TCAACGC 8815 0.0 29.749773 4 CAACGCA 8920 0.0 29.399582 5 AACGCAG 9230 0.0 28.456583 6 ACGCAGA 10555 0.0 24.706974 7 CGCAGAG 10590 0.0 24.535606 8 GTACCTG 1185 0.0 23.65713 1 GAGTACT 6610 0.0 23.642853 12-13 GCAGAGT 11165 0.0 22.889112 9 TACCTGG 1165 0.0 22.83647 2 TAGGACG 125 2.2536819E-4 22.803846 4 GTACATG 5705 0.0 22.570543 1 TACATGG 5800 0.0 21.95198 2 GTACTTT 7110 0.0 21.844265 14-15 ACATGGG 5825 0.0 20.87906 3 TAGACCG 115 0.003720056 20.655659 4 CAGAGTA 10955 0.0 20.401056 10-11 >>END_MODULE