FastQCFastQC Report
Thu 26 May 2016
SRR937873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304123
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62930.4825465082664748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53470.4100073382648723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33240.25488393349400323No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19460.14921905372422695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.10643167860700255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.042.5956461
GTATCAA64200.036.505571
ATCAACG78350.029.1722283
TATCAAC79050.029.0975952
TCAACGC79150.028.9407484
CAACGCA80150.028.462175
AACGCAG82350.027.6943426
GTACATG55800.024.8768791
CGCAGAG90850.024.840798
ACGCAGA91600.024.7411357
TACATGG57000.023.6786582
GCAGAGT95600.022.9090149
ACATGGG56950.022.7788833
GTACCTG11150.022.5968691
GAGTACT52300.022.4773512-13
TACCTGG11350.021.3544272
GTACTTT55850.020.8789314-15
TATCACG1150.003712625220.662642
AGTACTT55500.020.15438312-13
AGAGTAC85400.020.02432610-11
Produced by FastQC (version 0.11.5)