##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937873_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1304123 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502017064341324 33.0 31.0 34.0 30.0 34.0 2 31.63124337198255 33.0 31.0 34.0 30.0 34.0 3 31.636265137567545 33.0 31.0 34.0 30.0 34.0 4 35.236943141099424 37.0 35.0 37.0 33.0 37.0 5 35.2217206505828 37.0 35.0 37.0 33.0 37.0 6 35.26197528914067 37.0 35.0 37.0 33.0 37.0 7 35.193454911844974 37.0 35.0 37.0 32.0 37.0 8 35.17847856375511 37.0 35.0 37.0 32.0 37.0 9 36.768376909233254 39.0 37.0 39.0 33.0 39.0 10-11 36.639850688930416 39.0 37.0 39.0 32.0 39.0 12-13 36.49985430822093 39.0 37.0 39.0 32.0 39.0 14-15 37.60134856911503 40.0 37.0 41.0 32.0 41.0 16-17 37.51558902036081 40.0 37.0 41.0 32.0 41.0 18-19 37.45917332950957 40.0 37.0 41.0 32.0 41.0 20-21 37.37493434284956 40.0 37.0 41.0 32.0 41.0 22-23 37.16910636496711 40.0 37.0 41.0 31.0 41.0 24-25 36.9909966314527 39.0 36.0 41.0 31.0 41.0 26-27 36.928522846387956 39.0 36.0 41.0 31.0 41.0 28-29 36.959818974130506 39.0 36.0 41.0 31.0 41.0 30-31 36.76872465250594 39.0 36.0 41.0 30.0 41.0 32-33 36.50956734909207 39.0 35.5 41.0 30.0 41.0 34-35 36.5212863357214 39.0 36.0 41.0 30.0 41.0 36-37 36.416640915005715 39.0 35.0 41.0 30.0 41.0 38-39 36.27643941560727 39.0 35.0 41.0 30.0 41.0 40-41 36.03297503379666 39.0 35.0 41.0 29.0 41.0 42-43 35.77243480867986 39.0 35.0 41.0 27.5 41.0 44-45 35.520438639606844 39.0 35.0 40.0 27.0 41.0 46-47 35.163180543553025 39.0 35.0 40.0 25.5 41.0 48-49 34.79487172605651 38.0 34.0 40.0 24.0 41.0 50-51 33.85675009182416 37.5 33.5 39.5 22.5 40.5 52-53 33.95948081584329 38.0 33.5 39.5 23.0 40.5 54-55 34.29526969465304 38.0 34.0 40.0 23.0 41.0 56-57 34.082580784174496 38.0 34.0 40.0 22.0 41.0 58-59 33.82646038755547 38.0 33.0 40.0 20.5 41.0 60-61 33.50334362632972 38.0 33.0 40.0 18.5 41.0 62-63 33.13166472794361 37.0 32.5 40.0 17.5 41.0 64-65 32.75239912186197 36.5 32.0 39.5 13.5 41.0 66-67 32.338629101702836 36.0 31.5 39.0 10.5 41.0 68-69 31.849716629489706 35.5 31.0 39.0 8.0 40.0 70-71 31.40409992002288 35.0 31.0 38.5 7.0 40.0 72-73 30.925810295501268 35.0 30.0 38.0 2.0 40.0 74-75 30.44702263513488 35.0 30.0 37.0 2.0 39.0 76-77 29.941520086678942 34.0 29.0 36.5 2.0 39.0 78-79 29.397665710979716 34.0 29.0 36.0 2.0 38.5 80-81 28.849369653015856 34.0 28.5 35.5 2.0 37.5 82-83 28.293972654419868 34.0 27.0 35.0 2.0 37.0 84-85 27.808222844010878 34.0 26.0 35.0 2.0 36.5 86-87 27.308873472824267 33.0 26.0 35.0 2.0 36.0 88-89 26.797231549478077 33.0 25.0 35.0 2.0 36.0 90-91 26.270696475715866 32.5 24.5 35.0 2.0 35.0 92-93 25.66185206456753 32.0 21.5 35.0 2.0 35.0 94-95 25.004713129053012 32.0 19.0 34.0 2.0 35.0 96-97 24.246051561087413 31.5 8.5 34.0 2.0 35.0 98-99 23.271658424857165 31.0 2.0 34.0 2.0 35.0 100-101 20.958075656974074 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0180906566348753 1101 2 0.1078458317074471 1101 3 -0.045930773811790004 1101 4 0.018512295809316015 1101 5 -0.12198342505246274 1101 6 0.01645880549480694 1101 7 0.12233295291591162 1101 8 -0.10783124284203183 1101 9 0.09140510064970897 1101 10-11 0.14011718051069266 1101 12-13 0.20944264377323663 1101 14-15 0.3026594833834295 1101 16-17 0.38757077787155936 1101 18-19 0.2667862653167177 1101 20-21 0.3565819845922178 1101 22-23 0.35260355393815246 1101 24-25 0.3609728488441135 1101 26-27 0.3754073250600598 1101 28-29 0.23424661798510016 1101 30-31 0.15926901388179715 1101 32-33 0.2558393089157178 1101 34-35 0.38378136583393285 1101 36-37 0.41593691001887123 1101 38-39 0.4140547790773468 1101 40-41 0.47189466145234604 1101 42-43 0.3317895479016144 1101 44-45 0.47908789410620045 1101 46-47 0.673130921703148 1101 48-49 0.55615785522577 1101 50-51 0.5084515802183986 1101 52-53 0.5756182122752733 1101 54-55 0.7526613175725814 1101 56-57 0.7722065210188944 1101 58-59 0.6739975079468863 1101 60-61 0.6125249700799031 1101 62-63 0.7735548496040039 1101 64-65 0.6345144594909868 1101 66-67 0.8156663804805575 1101 68-69 0.7541016929295132 1101 70-71 0.7352640135172521 1101 72-73 0.8625830836961619 1101 74-75 0.8599676833129557 1101 76-77 0.7969734858822441 1101 78-79 0.8874912169936451 1101 80-81 0.9215137560255648 1101 82-83 1.169726659930479 1101 84-85 1.2161811720812992 1101 86-87 1.2226315782913808 1101 88-89 1.1136286225624907 1101 90-91 1.4644630904554887 1101 92-93 1.6060334721685727 1101 94-95 1.6957771484735993 1101 96-97 1.7087355651973155 1101 98-99 1.617202954098456 1101 100-101 2.0188622763969875 1102 1 0.14283982530819017 1102 2 0.05528950215808237 1102 3 0.1839704703115892 1102 4 0.11226946241865221 1102 5 0.03965228186188341 1102 6 0.028269377676892304 1102 7 0.07238885246871263 1102 8 0.18209035445185862 1102 9 0.226897016714382 1102 10-11 0.1476564535298408 1102 12-13 0.29809001792757783 1102 14-15 0.24372845483166117 1102 16-17 0.3456281967455439 1102 18-19 0.2085701060459897 1102 20-21 0.26389085112128186 1102 22-23 0.4407707652671178 1102 24-25 0.4298063398428482 1102 26-27 0.22986065610007955 1102 28-29 0.13514495246509028 1102 30-31 0.29627845947289444 1102 32-33 0.18840151508473468 1102 34-35 0.4103121535876326 1102 36-37 0.43100291449083983 1102 38-39 0.37889539368576663 1102 40-41 0.48061642501156854 1102 42-43 0.566800584479985 1102 44-45 0.4936092362687958 1102 46-47 0.35870310379236514 1102 48-49 0.41704680144753326 1102 50-51 0.3948411141021282 1102 52-53 0.31405309646287094 1102 54-55 0.34065832192988665 1102 56-57 0.45126604687601457 1102 58-59 0.2577411296645806 1102 60-61 0.43225966220236955 1102 62-63 0.5320866980158883 1102 64-65 0.42819673503277755 1102 66-67 0.6737908071518106 1102 68-69 0.6342616516498474 1102 70-71 0.5984498580304916 1102 72-73 0.5655110019394805 1102 74-75 0.6894292559537085 1102 76-77 0.6205279807796202 1102 78-79 0.5541328005276434 1102 80-81 0.7072643866871893 1102 82-83 0.5425713021733394 1102 84-85 0.6679713910928768 1102 86-87 0.8329077794151054 1102 88-89 0.7862704497612079 1102 90-91 0.6816203115310557 1102 92-93 0.8015367831420832 1102 94-95 0.8399066204378265 1102 96-97 0.7180324491555794 1102 98-99 0.9926024925688139 1102 100-101 1.6595034012679903 1103 1 0.12291269736889276 1103 2 0.20575313588743782 1103 3 0.23829181570798497 1103 4 0.1429154372836976 1103 5 0.10554300596761834 1103 6 0.07621307622911644 1103 7 0.09829116741768473 1103 8 0.17121857278900166 1103 9 0.06537378673273508 1103 10-11 0.15200033667697 1103 12-13 0.16151661461989164 1103 14-15 0.27382842585454625 1103 16-17 0.3702600801502456 1103 18-19 0.32639424719670274 1103 20-21 0.2152737031126506 1103 22-23 0.19631934485221336 1103 24-25 0.3012955485531563 1103 26-27 0.21151519531039042 1103 28-29 0.16932452467711556 1103 30-31 0.20292655932586712 1103 32-33 0.2748163492176232 1103 34-35 0.2408161942843705 1103 36-37 0.2731018230197648 1103 38-39 0.24218112943600545 1103 40-41 0.17769832290482412 1103 42-43 0.24345898730471305 1103 44-45 -0.022258413473089433 1103 46-47 -0.038662930744052915 1103 48-49 0.2127112358036527 1103 50-51 0.3508039590102001 1103 52-53 0.18861307371047076 1103 54-55 0.13026976037499338 1103 56-57 0.06554988301583364 1103 58-59 0.1646588776066693 1103 60-61 0.4163745725343233 1103 62-63 0.5137251388328252 1103 64-65 0.40971980403049457 1103 66-67 0.37259255007121084 1103 68-69 0.3619935490064883 1103 70-71 0.35592756175566365 1103 72-73 0.35082551088482106 1103 74-75 0.3692577007415885 1103 76-77 0.432242513335936 1103 78-79 0.5388259114885052 1103 80-81 0.356945906804814 1103 82-83 0.45838877858919247 1103 84-85 0.5510179047174866 1103 86-87 0.5736122987707439 1103 88-89 0.7239830827071039 1103 90-91 0.9500543134843262 1103 92-93 0.9561216055768043 1103 94-95 0.8300302560989863 1103 96-97 1.2153131622070816 1103 98-99 1.2386087494777058 1103 100-101 1.544617216909188 1104 1 0.3243080582470128 1104 2 0.35220038362778894 1104 3 0.31589651400233976 1104 4 0.1769849131635155 1104 5 0.33800946257200337 1104 6 -0.019992119114014884 1104 7 0.14755225116152815 1104 8 0.2360965665463084 1104 9 0.2383349252111131 1104 10-11 0.3392340605486268 1104 12-13 0.358358006504389 1104 14-15 0.5277661690164237 1104 16-17 0.5987342439692398 1104 18-19 0.4861215545533213 1104 20-21 0.5824962211972391 1104 22-23 0.6223107611453571 1104 24-25 0.4299406653448159 1104 26-27 0.39618138859727736 1104 28-29 0.4191017033336095 1104 30-31 0.4764439190501193 1104 32-33 0.5090843302528398 1104 34-35 0.6116292795371052 1104 36-37 0.569008567202367 1104 38-39 0.692044940291197 1104 40-41 0.5816623238515817 1104 42-43 0.7214736398883375 1104 44-45 0.8458419837221385 1104 46-47 0.8702918984693895 1104 48-49 0.7023468073357719 1104 50-51 0.6285840482127085 1104 52-53 0.7343649524364864 1104 54-55 0.6556737049599732 1104 56-57 0.7806450582403244 1104 58-59 0.6948234325557081 1104 60-61 0.8027661981500813 1104 62-63 0.8525763052701976 1104 64-65 0.5237021669352728 1104 66-67 0.705832039560697 1104 68-69 0.8011767284913951 1104 70-71 0.6267410168363625 1104 72-73 0.6048113316809882 1104 74-75 0.7723743333840822 1104 76-77 0.7501355295048135 1104 78-79 0.7530228670647681 1104 80-81 1.009603490496879 1104 82-83 1.2611280942595435 1104 84-85 1.29025359977215 1104 86-87 1.2722552150860729 1104 88-89 1.1809813371191495 1104 90-91 1.308324221323197 1104 92-93 1.3139296907555824 1104 94-95 1.1912800526936103 1104 96-97 1.4768795315320737 1104 98-99 1.8286701779012056 1104 100-101 2.1259183456241075 1105 1 0.09906459342646201 1105 2 0.007739208295287625 1105 3 0.07662004497182195 1105 4 0.0715671453657265 1105 5 0.007699848819079591 1105 6 0.07547594943444125 1105 7 0.1675482519613638 1105 8 0.10165284718206635 1105 9 0.02777703678879817 1105 10-11 0.17060055751949932 1105 12-13 0.28703797996915625 1105 14-15 0.2579427688316045 1105 16-17 0.40104472781840883 1105 18-19 0.35359012165071135 1105 20-21 0.4889007511020722 1105 22-23 0.33228886687555814 1105 24-25 0.32073632378070016 1105 26-27 0.23156701417486403 1105 28-29 0.4019507416340602 1105 30-31 0.559851561845889 1105 32-33 0.3391515938271539 1105 34-35 0.22131122828249516 1105 36-37 0.220810639220403 1105 38-39 0.33598355121765167 1105 40-41 0.2534350638981735 1105 42-43 0.31432297542662724 1105 44-45 0.396839047822823 1105 46-47 0.4341260910903273 1105 48-49 0.3648730858638487 1105 50-51 0.41666714105360114 1105 52-53 0.31920288349891734 1105 54-55 0.4081645919177177 1105 56-57 0.524192801394392 1105 58-59 0.4503789092982018 1105 60-61 0.48396745789813167 1105 62-63 0.5370610299468837 1105 64-65 0.5237114556180771 1105 66-67 0.4302143590427434 1105 68-69 0.42354938233900796 1105 70-71 0.4774132810321703 1105 72-73 0.4386739510490081 1105 74-75 0.31512878924966614 1105 76-77 0.4185626548905468 1105 78-79 0.48523318175858776 1105 80-81 0.5673000592912274 1105 82-83 0.5620375069715955 1105 84-85 0.8132150547784427 1105 86-87 0.5951652952322029 1105 88-89 0.7128161863655187 1105 90-91 0.7021153955207922 1105 92-93 0.7788128965468566 1105 94-95 1.007509374396836 1105 96-97 0.9484453535028123 1105 98-99 1.0946240817150361 1105 100-101 1.4418733978569058 1106 1 0.08463314236727726 1106 2 0.01376556326525602 1106 3 0.10561620357142587 1106 4 -0.051299847500764884 1106 5 0.05506688143665173 1106 6 -0.06030879120779531 1106 7 -0.1375883110874696 1106 8 -0.20715617414619203 1106 9 0.010172274608706289 1106 10-11 -0.39713709976831524 1106 12-13 -0.24558139976935678 1106 14-15 -0.46454089708581847 1106 16-17 -0.4153118050918678 1106 18-19 -0.012148552903525456 1106 20-21 0.09100322217412327 1106 22-23 6.959234160603955E-4 1106 24-25 -0.10976206449345227 1106 26-27 0.03648584968487256 1106 28-29 0.08461850025100404 1106 30-31 0.10477141823605507 1106 32-33 0.11544219414083301 1106 34-35 0.0979868720693915 1106 36-37 0.1487464376028882 1106 38-39 0.14059250894418085 1106 40-41 0.17028030930692495 1106 42-43 0.11403035322818056 1106 44-45 0.22369461044524286 1106 46-47 0.15063964079002545 1106 48-49 0.24141863518772766 1106 50-51 0.05664765398715588 1106 52-53 0.24252597490473704 1106 54-55 0.17139876737177673 1106 56-57 0.2688410855929817 1106 58-59 0.23212805645670898 1106 60-61 0.18228427043924 1106 62-63 0.5181846847339955 1106 64-65 0.49435844067889434 1106 66-67 0.5389245447164583 1106 68-69 0.5124508791480729 1106 70-71 0.5752885854893464 1106 72-73 0.6262925267314579 1106 74-75 0.7250431166251552 1106 76-77 0.8144909240559883 1106 78-79 1.041660525807849 1106 80-81 1.0274044239604656 1106 82-83 0.6587320234899785 1106 84-85 0.5812995402909777 1106 86-87 0.9159244867673912 1106 88-89 0.8666836056527423 1106 90-91 1.0792016499566515 1106 92-93 1.0672368382645345 1106 94-95 1.3075763188392742 1106 96-97 1.34626925257842 1106 98-99 1.6751947898625659 1106 100-101 1.9270146226240001 1107 1 0.12425406892130653 1107 2 0.1440189412448447 1107 3 0.14773815315945882 1107 4 0.24050879792351765 1107 5 0.12569754255270027 1107 6 0.13139056664163462 1107 7 0.1786865860189195 1107 8 0.2815796312569958 1107 9 0.2951605293913886 1107 10-11 0.39949357708671585 1107 12-13 0.3611584269444208 1107 14-15 0.5199645643418691 1107 16-17 0.4757717157976984 1107 18-19 0.39952992128059606 1107 20-21 0.2001216029473767 1107 22-23 0.4610634385451533 1107 24-25 0.565504809665498 1107 26-27 0.47888214944956786 1107 28-29 0.4225790131058247 1107 30-31 0.5589284283798364 1107 32-33 0.45960189958572784 1107 34-35 0.4771471283787676 1107 36-37 0.36056857920653584 1107 38-39 0.4605070462573764 1107 40-41 0.3270081350448848 1107 42-43 0.42069020622606246 1107 44-45 0.49578575097076794 1107 46-47 0.593788214838014 1107 48-49 0.4424529324027091 1107 50-51 0.4397613465103518 1107 52-53 0.45589422583137207 1107 54-55 0.6453342709799443 1107 56-57 0.7700448719992181 1107 58-59 0.6988334639783815 1107 60-61 0.9380127690187976 1107 62-63 0.9313389346533398 1107 64-65 0.9540336864037968 1107 66-67 0.7796975649489184 1107 68-69 0.8003247784028353 1107 70-71 1.0236322540892928 1107 72-73 1.06004836982952 1107 74-75 1.0309782385629376 1107 76-77 1.0630308873054624 1107 78-79 1.219164057242537 1107 80-81 1.2192232997247885 1107 82-83 1.2018391351502054 1107 84-85 1.2623849203481115 1107 86-87 1.5493591070250403 1107 88-89 1.5110907574473806 1107 90-91 1.5056511612611772 1107 92-93 1.5788118778087359 1107 94-95 1.7622554206154177 1107 96-97 1.6988161154053003 1107 98-99 2.0273144868084145 1107 100-101 2.4716375952460368 1108 1 0.11870454503480232 1108 2 -0.015323871396464739 1108 3 0.05722044663061965 1108 4 0.07233314601175067 1108 5 0.20504673395710427 1108 6 0.21773498926526713 1108 7 -0.014543623977829157 1108 8 0.03818875070370353 1108 9 0.1119135205288373 1108 10-11 -0.014010613891699109 1108 12-13 0.11178854825014639 1108 14-15 0.1195733189757533 1108 16-17 0.2561696894481642 1108 18-19 0.2804218121687967 1108 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16-17 -0.07701856363288329 2316 18-19 -0.15284318072990288 2316 20-21 0.05269244481812052 2316 22-23 0.15639921603860785 2316 24-25 0.3120175195963313 2316 26-27 0.14814825272167553 2316 28-29 0.16872375689401764 2316 30-31 0.20561829890943528 2316 32-33 0.32883858793518783 2316 34-35 0.01804896977528614 2316 36-37 0.030967101078715586 2316 38-39 -0.2664156607207815 2316 40-41 -0.34978281992232496 2316 42-43 -0.3445127231870728 2316 44-45 -0.411589677391035 2316 46-47 -0.39693937389886713 2316 48-49 -0.5152192175932626 2316 50-51 -0.11396115109619132 2316 52-53 -0.025180123461325365 2316 54-55 -0.3694134541169163 2316 56-57 -0.5662750639366436 2316 58-59 -0.5015274560040908 2316 60-61 -0.35733654157594685 2316 62-63 -0.26125499178027667 2316 64-65 -0.147280031016102 2316 66-67 -0.05168446667156701 2316 68-69 -0.15048854980059545 2316 70-71 -0.020931912942735664 2316 72-73 0.28726690369135355 2316 74-75 0.03043076310147086 2316 76-77 -0.03366368066426162 2316 78-79 -0.3041318904324548 2316 80-81 -0.11263518653631976 2316 82-83 0.008335918522163865 2316 84-85 -0.06259796754859792 2316 86-87 0.011557016887874028 2316 88-89 -0.2932334598696187 2316 90-91 -0.33787934525707897 2316 92-93 -0.2810467068749283 2316 94-95 0.1459881854839331 2316 96-97 0.5449715961994812 2316 98-99 0.8330127053601686 2316 100-101 1.5480845186464478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6865.0 3 1603.0 4 1542.0 5 1690.0 6 1990.0 7 2220.0 8 2611.0 9 3235.0 10 3960.0 11 4869.0 12 6197.0 13 7651.0 14 8497.0 15 9949.0 16 12130.0 17 15150.0 18 13823.0 19 10299.0 20 10060.0 21 10860.0 22 11605.0 23 12493.0 24 14116.0 25 15992.0 26 18480.0 27 21217.0 28 24674.0 29 29543.0 30 35307.0 31 43517.0 32 53959.0 33 68672.0 34 90533.0 35 124575.0 36 175033.0 37 230917.0 38 176747.0 39 21538.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.452652922453034 19.128902385930257 15.072405575837013 22.346039115779693 2 15.018568834658543 19.945827760888474 41.63479574281876 23.400807661634225 3 19.030698047816085 24.770058772370295 30.126856966213765 26.072386213599845 4 12.37941467052632 16.911662380298853 38.052335974350996 32.65658697482384 5 13.393323943142189 37.335159089930556 34.44805973637756 14.823457230549694 6 30.748338843623053 36.536141198676304 18.085201221639686 14.630318736060966 7 27.63732907107646 33.171910600186735 21.018254190856382 18.172506137880422 8 25.796311979993636 35.3944785756994 19.911987986344766 18.8972214579622 9 25.93266074520633 17.17105652491694 20.950425894770984 35.94585683510575 10-11 24.571564939399423 27.09377400610175 27.433985742080846 20.90067531241798 12-13 25.462300638073444 24.501021186497667 28.187437196550064 21.84924097887883 14-15 22.497980708561872 25.830099777205483 26.788715638422715 24.883203875809926 16-17 21.439618698205116 29.209096187327876 27.408141472679816 21.94314364178719 18-19 21.281551846106495 27.472359412747277 30.208040737420472 21.03804800372576 20-21 22.157693597719692 26.5220586851042 30.352961245301707 20.967286471874402 22-23 21.748314104280617 26.516230432064923 30.235170591170913 21.500284872483547 24-25 21.523592627296768 26.64055017512802 30.405466406807964 21.430390790767255 26-27 21.428038997655438 26.88004924272406 30.224028266921998 21.467883492698505 28-29 21.192742129480546 26.930537761508443 30.484270489327542 21.392449619683465 30-31 21.411982779423226 26.759791090610914 30.402756153005534 21.425469976960333 32-33 21.218529293882607 26.803394380588564 30.296034163611356 21.682042161917472 34-35 21.63799473349915 26.852583701666905 30.120918951756533 21.388502613077414 36-37 21.584268652794204 26.772342974288993 30.038949553067518 21.60443881984929 38-39 21.46776308535076 26.96304754498753 30.143535154951806 21.425654214709905 40-41 21.604676223753387 26.9099926813948 30.01273552558534 21.472595569266467 42-43 21.494084653372713 26.9454403360899 29.823060226992855 21.737414783544537 44-45 21.56879620095591 27.109453042155252 29.639651754291595 21.682099002597244 46-47 21.660562298556812 27.22219041863299 29.265620119403053 21.851627163407137 48-49 21.75400064322297 27.2937488640813 29.1077990208717 21.844451471824026 50-51 21.82728281620344 27.50250546437168 28.862603715465323 21.807608003959555 52-53 21.930555112516366 27.59624700756386 28.71228300634479 21.760914873574976 54-55 21.672100346000818 27.555305377901675 28.76025659005723 22.01233768604028 56-57 21.761046007119507 27.857875726345817 28.641505244747854 21.73957302178682 58-59 21.864024532420835 27.477509834825636 28.821599470507813 21.836866162245716 60-61 21.862467936934536 27.465115269529516 29.069872416178878 21.60254437735707 62-63 21.73540040401929 27.52813650903665 29.045475606317083 21.690987480626976 64-65 21.667276183217602 27.43573569928195 28.951972144581422 21.945015972919027 66-67 21.597590514186273 27.719382492427137 28.733540910741073 21.949486082645517 68-69 21.619899012756143 27.605830874220054 28.82957989598537 21.94469021703844 70-71 21.708587809279994 27.56280872295781 28.681568091731968 22.04703537603023 72-73 21.728507277539187 27.55491817354454 28.648652386526518 22.06792216238976 74-75 21.609583291553765 27.836832636238846 28.464774734176302 22.088809338031083 76-77 21.78051006195668 27.686816195187554 28.410662312088757 22.122011430767014 78-79 21.74798833250142 27.673920235716977 28.441621968281343 22.13646946350026 80-81 21.64810769149197 27.928984352367202 28.384243403181475 22.038664552959357 82-83 21.68483796443044 27.853589172296328 28.46659912222969 21.99497374104354 84-85 21.814024929040905 27.65756607609643 28.357168213398243 22.171240781464423 86-87 21.724387652978702 27.660624035929228 28.460925239012713 22.154063072079357 88-89 21.804946324719598 27.56359447836756 28.413569946914475 22.21788924999837 90-91 21.766800282317973 27.66769929677928 28.438656353940985 22.126844066961766 92-93 21.85119999200863 27.475123991041983 28.535439980421152 22.138236036528227 94-95 21.66379667388199 27.605872765958427 28.39433316658944 22.33599739357014 96-97 21.76905609697912 27.562917840419775 28.379438957219367 22.28858710538174 98-99 21.79205350220787 27.497293463820778 28.202337494496643 22.508315539474705 100-101 22.030119928839433 27.038903595560264 28.56802607018405 22.362950405416253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2052.0 1 1245.0 2 460.5 3 467.0 4 374.0 5 348.0 6 592.5 7 1179.0 8 1917.5 9 2271.5 10 1994.5 11 1673.0 12 1698.0 13 1824.5 14 1995.0 15 2148.0 16 2248.0 17 2343.0 18 2487.5 19 2705.5 20 2995.0 21 3454.5 22 4002.0 23 4717.5 24 5832.5 25 7212.5 26 8861.5 27 10977.0 28 12801.0 29 14721.0 30 17195.0 31 19335.5 32 21559.0 33 24308.0 34 27517.0 35 30270.0 36 33389.0 37 36456.0 38 38343.5 39 39866.5 40 41099.5 41 42818.0 42 44476.0 43 46186.5 44 48064.5 45 49094.5 46 49370.0 47 49449.5 48 51013.0 49 50748.5 50 47927.5 51 45169.5 52 42562.5 53 40665.5 54 38599.5 55 35414.0 56 31526.0 57 27903.5 58 24763.5 59 21704.5 60 18175.0 61 14758.0 62 11619.5 63 8835.5 64 6634.5 65 5043.5 66 3879.0 67 3071.0 68 2351.0 69 1647.0 70 1194.5 71 871.0 72 673.5 73 546.5 74 415.0 75 320.0 76 224.5 77 148.5 78 100.5 79 68.0 80 49.0 81 34.5 82 23.0 83 13.0 84 6.5 85 10.0 86 9.0 87 3.5 88 2.5 89 3.5 90 4.0 91 4.0 92 5.0 93 3.5 94 1.5 95 3.0 96 3.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31323732500692036 2 0.29337723512276065 3 0.28233533186670273 4 0.26745943442451364 5 0.26646259593611954 6 0.22398194035378566 7 0.21301671698145036 8 0.22520881849334762 9 0.22850605349342049 10-11 0.2316882686679094 12-13 0.22547719808637684 14-15 0.22421198000495351 16-17 0.22210328320258135 18-19 0.22325348145842075 20-21 0.20853094378367687 22-23 0.2042368702952099 24-25 0.20293331227192526 26-27 0.21577719279546487 28-29 0.22773925465619424 30-31 0.22152818407466168 32-33 0.2279309543655008 34-35 0.22079972517929672 36-37 0.20672896651619518 38-39 0.20968114203951618 40-41 0.20285663238820265 42-43 0.2150487339000999 44-45 0.21033292105115853 46-47 0.20952778227207097 48-49 0.21957284703973476 50-51 0.22750921500502638 52-53 0.24035309552856593 54-55 0.21665901145827504 56-57 0.190741210760028 58-59 0.19158468948097687 60-61 0.19833251924856782 62-63 0.2070356860510857 64-65 0.1951503040740789 66-67 0.19940603762068454 68-69 0.20485030936499088 70-71 0.19875425860904225 72-73 0.2007862755276918 74-75 0.20316335192309315 76-77 0.21550881320243567 78-79 0.22248668262119445 80-81 0.2128633572140051 82-83 0.2114447793651366 84-85 0.20465860965568433 86-87 0.20515702889988136 88-89 0.20952778227207097 90-91 0.21113805983024608 92-93 0.2084542638999542 94-95 0.2090293630278739 96-97 0.1966455618066701 98-99 0.20274161256261872 100-101 0.19626216238805694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1304123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.2065337273719 #Duplication Level Percentage of deduplicated Percentage of total 1 83.87460311135541 57.20795945984596 2 10.071217708159638 13.73845700574591 3 2.6614056666960595 5.4457576610317044 4 1.1347485302341143 3.095890555979953 5 0.5842989936536027 1.9926504508751959 6 0.35361843389087244 1.4471452582678934 7 0.24975467981172916 1.1924430694503374 8 0.16690129656513505 0.9107007130649587 9 0.13113992865750454 0.8050139970284906 >10 0.7146168287672363 8.86407253155394 >50 0.041139541838719276 1.8993491550911508 >100 0.0154290882690868 1.76711767261045 >500 5.630960504150995E-4 0.22790612776253483 >1k 3.3785763024905976E-4 0.5114253450094226 >5k 2.2523842016603982E-4 0.8941109966821386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6293 0.4825465082664748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5347 0.4100073382648723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3324 0.25488393349400323 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1946 0.14921905372422695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 0.10643167860700255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.667988372262432E-5 0.0 0.0 0.0 0.0 14-15 1.1501982558393648E-4 0.0 0.0 3.833994186131216E-5 0.0 16-17 1.5335976744524865E-4 0.0 0.0 1.5335976744524865E-4 0.0 18-19 1.5335976744524865E-4 0.0 0.0 1.5335976744524865E-4 0.0 20-21 2.3003965116787296E-4 0.0 0.0 1.916997093065608E-4 0.0 22-23 2.3003965116787296E-4 0.0 0.0 2.683795930291851E-4 0.0 24-25 2.3003965116787296E-4 0.0 0.0 5.750991279196824E-4 0.0 26-27 2.3003965116787296E-4 0.0 0.0 6.901189535036189E-4 0.0 28-29 2.3003965116787296E-4 0.0 0.0 0.0015335976744524863 0.0 30-31 3.4505947675180946E-4 0.0 0.0 0.003067195348904973 0.0 32-33 3.8339941861312163E-4 0.0 0.0 0.00513755220941583 0.0 34-35 4.217393604744338E-4 0.0 0.0 0.007169569128065375 0.0 36-37 4.600793023357459E-4 0.0 0.0 0.010735183721167406 0.0 38-39 4.984192441970581E-4 0.0 0.0 0.01614111552361242 0.0 40-41 6.134390697809946E-4 0.0 0.0 0.025112661919159465 0.0 42-43 6.134390697809946E-4 0.0 0.0 0.03212887127977959 0.0 44-45 6.134390697809946E-4 0.0 0.0 0.04125377744277189 0.0 46-47 6.134390697809946E-4 0.0 0.0 0.05881347081525286 0.0 48-49 6.901189535036189E-4 0.0 0.0 0.07238581023415736 0.0 50-51 6.901189535036189E-4 0.0 0.0 0.08753008726937567 0.0 52-53 6.901189535036189E-4 0.0 0.0 0.10451468151393695 0.0 54-55 6.901189535036189E-4 0.0 0.0 0.12594670901441046 0.0 56-57 6.901189535036189E-4 0.0 0.0 0.1717629395386785 0.0 58-59 6.901189535036189E-4 0.0 0.0 0.21604557238849403 0.0 60-61 6.901189535036189E-4 0.0 0.0 0.25361871541257996 0.0 62-63 6.901189535036189E-4 0.0 0.0 0.2993582660531253 0.0 64-65 6.901189535036189E-4 0.0 0.0 0.34655473448440066 0.0 66-67 6.901189535036189E-4 0.0 0.0 0.4150298706487041 0.0 68-69 6.901189535036189E-4 0.0 0.0 0.4988026436156712 0.0 70-71 6.901189535036189E-4 0.0 0.0 0.5523635423959243 0.0 72-73 6.901189535036189E-4 0.0 0.0 0.6145125881531113 0.0 74-75 6.901189535036189E-4 0.0 0.0 0.7114743011203698 0.0 76-77 6.901189535036189E-4 0.0 0.0 0.8223150730414233 0.0 78-79 6.901189535036189E-4 0.0 0.0 0.94523292664879 0.0 80-81 6.901189535036189E-4 0.0 0.0 1.0603294321164491 0.0 82-83 6.901189535036189E-4 0.0 0.0 1.2031840554916982 0.0 84-85 6.901189535036189E-4 0.0 0.0 1.3730683378791724 0.0 86-87 6.901189535036189E-4 0.0 0.0 1.5564099398599671 0.0 88-89 6.901189535036189E-4 0.0 0.0 1.7998685706792994 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3895 0.0 42.595646 1 GTATCAA 6420 0.0 36.50557 1 ATCAACG 7835 0.0 29.172228 3 TATCAAC 7905 0.0 29.097595 2 TCAACGC 7915 0.0 28.940748 4 CAACGCA 8015 0.0 28.46217 5 AACGCAG 8235 0.0 27.694342 6 GTACATG 5580 0.0 24.876879 1 CGCAGAG 9085 0.0 24.84079 8 ACGCAGA 9160 0.0 24.741135 7 TACATGG 5700 0.0 23.678658 2 GCAGAGT 9560 0.0 22.909014 9 ACATGGG 5695 0.0 22.778883 3 GTACCTG 1115 0.0 22.596869 1 GAGTACT 5230 0.0 22.47735 12-13 TACCTGG 1135 0.0 21.354427 2 GTACTTT 5585 0.0 20.87893 14-15 TATCACG 115 0.0037126252 20.66264 2 AGTACTT 5550 0.0 20.154383 12-13 AGAGTAC 8540 0.0 20.024326 10-11 >>END_MODULE