FastQCFastQC Report
Thu 26 May 2016
SRR937873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304123
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73330.5622935873380042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66450.5095378273368386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.32703970407699273No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.1346498758169283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.046.3916131
GTATCAA68150.036.117581
ATCAACG87350.027.7883513
TCAACGC88400.027.458294
TATCAAC88700.027.4189722
CAACGCA89850.027.0151635
AACGCAG92700.026.1825946
CGTAGAC950.001226458825.0006473
ATAACGC950.001226458825.0006473
GTACCTG10500.023.532541
ACGCAGA102700.023.4953257
GTACATG54450.023.2133851
CGCAGAG103700.023.131348
TACATGG55250.022.6114442
ACATGGG54650.022.251263
GAGTACT65700.022.01456512-13
TACCTGG11300.021.4386082
GCAGAGT109900.021.43749
AGTACTT68200.021.0334612-13
GTACTTT69400.020.874314-15
Produced by FastQC (version 0.11.5)