##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937873_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1304123 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18360384718313 33.0 31.0 34.0 31.0 34.0 2 32.392494419621464 34.0 31.0 34.0 31.0 34.0 3 32.44944917005528 34.0 31.0 34.0 31.0 34.0 4 36.06688326177822 37.0 35.0 37.0 35.0 37.0 5 35.975944753677375 37.0 35.0 37.0 35.0 37.0 6 36.021030224909765 37.0 35.0 37.0 35.0 37.0 7 35.975187923225036 37.0 35.0 37.0 35.0 37.0 8 35.94142347002545 37.0 35.0 37.0 35.0 37.0 9 37.62652679233477 39.0 37.0 39.0 35.0 39.0 10-11 37.54065222375497 39.0 37.0 39.0 35.0 39.0 12-13 37.43045134546358 39.0 37.0 39.0 34.0 39.0 14-15 38.630233881313345 40.0 38.0 41.0 34.0 41.0 16-17 38.58562037476527 40.0 38.0 41.0 34.0 41.0 18-19 38.570353409916095 40.0 38.0 41.0 34.0 41.0 20-21 38.46749501389056 40.0 38.0 41.0 34.0 41.0 22-23 38.41398855782775 40.0 38.0 41.0 34.0 41.0 24-25 38.426336319503605 40.0 38.0 41.0 34.0 41.0 26-27 38.23749638646048 40.0 38.0 41.0 34.0 41.0 28-29 38.20346892125973 40.0 38.0 41.0 33.5 41.0 30-31 38.205493270190004 40.0 38.0 41.0 34.0 41.0 32-33 38.06932935006897 40.0 37.0 41.0 33.5 41.0 34-35 37.91597648381326 40.0 37.0 41.0 33.0 41.0 36-37 37.73034407030625 40.0 37.0 41.0 33.0 41.0 38-39 37.581914819384366 40.0 37.0 41.0 33.0 41.0 40-41 37.395785903630255 40.0 37.0 41.0 32.5 41.0 42-43 37.16416166266525 40.0 36.0 41.0 31.5 41.0 44-45 36.84950652660831 40.0 36.0 41.0 31.0 41.0 46-47 36.488981100709054 39.0 36.0 41.0 30.5 41.0 48-49 36.12820799878539 39.0 35.0 41.0 29.5 41.0 50-51 35.872286969864035 39.0 35.0 40.5 29.0 41.0 52-53 35.60006763165744 39.0 35.0 40.0 28.0 41.0 54-55 35.36289675130337 38.5 35.0 40.0 27.5 41.0 56-57 35.085605038788515 38.0 34.5 40.0 27.0 41.0 58-59 34.829363871352626 38.0 34.0 40.0 26.0 41.0 60-61 34.48252273750252 38.0 34.0 40.0 26.0 41.0 62-63 34.06267737015604 37.0 33.5 40.0 25.0 41.0 64-65 33.66791667657115 37.0 33.0 39.5 23.5 41.0 66-67 33.24218957874372 36.0 32.5 39.0 22.5 40.0 68-69 32.76868324536872 36.0 32.0 39.0 22.0 40.0 70-71 32.33498565702775 35.0 31.5 38.0 20.5 40.0 72-73 31.846362651375674 35.0 31.0 37.5 19.5 39.5 74-75 31.357023839009052 35.0 31.0 37.0 18.0 39.0 76-77 30.103425827165076 33.5 29.5 35.5 17.5 38.0 78-79 30.71018799607092 34.0 30.5 36.0 17.5 38.5 80-81 30.527104038499438 34.0 30.5 36.0 14.0 37.5 82-83 30.125303748189395 34.0 30.0 35.0 9.0 37.0 84-85 29.69361363920428 34.0 29.5 35.0 7.0 36.5 86-87 29.311309209330716 34.0 29.0 35.0 2.0 36.0 88-89 28.877103616760074 34.0 29.0 35.0 2.0 36.0 90-91 28.35105047606706 33.5 29.0 35.0 2.0 35.0 92-93 27.850184376780412 33.0 27.0 35.0 2.0 35.0 94-95 27.31326301276797 33.0 26.5 35.0 2.0 35.0 96-97 26.738592141998875 33.0 25.5 34.5 2.0 35.0 98-99 25.856856293463117 32.0 24.5 34.0 2.0 35.0 100-101 23.35388111397468 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3282219181588317 1101 2 0.3039163611748137 1101 3 0.20936939458983517 1101 4 0.21972975819209495 1101 5 0.18046269993651975 1101 6 0.14085766211696438 1101 7 0.18009589600960396 1101 8 0.1806976942447278 1101 9 0.3012970140255433 1101 10-11 0.2044271202145751 1101 12-13 0.3161272085312561 1101 14-15 0.3734980214066681 1101 16-17 0.381607544588654 1101 18-19 0.38106836752062634 1101 20-21 0.3903507036209817 1101 22-23 0.3221645644148694 1101 24-25 0.3335341510685126 1101 26-27 0.2584747134352625 1101 28-29 0.344635412071284 1101 30-31 0.46408621009952356 1101 32-33 0.5070739341096768 1101 34-35 0.3766830346252803 1101 36-37 0.32281962568850275 1101 38-39 0.34713032737408156 1101 40-41 0.48881947486423627 1101 42-43 0.5272073014455145 1101 44-45 0.5362515041331974 1101 46-47 0.5566149370050084 1101 48-49 0.47128497163109984 1101 50-51 0.46303072809567425 1101 52-53 0.5362812469646556 1101 54-55 0.45968447806385626 1101 56-57 0.47187279196798926 1101 58-59 0.40914817186983043 1101 60-61 0.49636391692217074 1101 62-63 0.5571519775960496 1101 64-65 0.5526952265192548 1101 66-67 0.6415440092827751 1101 68-69 0.518568135383795 1101 70-71 0.3881678638018258 1101 72-73 0.5262757832518297 1101 74-75 0.8092853010374874 1101 76-77 0.5923622066508365 1101 78-79 0.7792810100796252 1101 80-81 0.8040186685505404 1101 82-83 0.6326331117184623 1101 84-85 0.695912413730408 1101 86-87 0.7720629620910167 1101 88-89 1.0239860545255262 1101 90-91 1.0078003313903885 1101 92-93 1.229449821901241 1101 94-95 1.2644138131123661 1101 96-97 1.236911585139282 1101 98-99 1.3340597453866962 1101 100-101 1.6021600438288033 1102 1 0.15404106160568887 1102 2 0.044101689692183754 1102 3 0.13171201584886916 1102 4 0.0992088890257179 1102 5 0.005235518420640517 1102 6 0.07721458792821778 1102 7 0.08599114884758308 1102 8 0.07595053222202353 1102 9 0.12489092527425072 1102 10-11 0.17732479536650914 1102 12-13 0.16998750264890106 1102 14-15 0.17505819225863206 1102 16-17 0.17500673906176445 1102 18-19 0.19862620205795167 1102 20-21 0.09392899166134328 1102 22-23 0.12701779225571386 1102 24-25 0.06356797746457943 1102 26-27 0.09836907761368252 1102 28-29 0.21336642055654664 1102 30-31 0.13089979225753012 1102 32-33 0.18363768826630888 1102 34-35 0.18275816935753397 1102 36-37 0.38192086121195246 1102 38-39 0.30549232714474783 1102 40-41 0.41037914405355025 1102 42-43 0.3674994124419584 1102 44-45 0.43807612286958175 1102 46-47 0.4209778971013236 1102 48-49 0.32644958864749896 1102 50-51 0.32637423801426024 1102 52-53 0.5194988395572437 1102 54-55 0.4667597131286456 1102 56-57 0.48194187808196887 1102 58-59 0.49395066011658884 1102 60-61 0.44935275134482566 1102 62-63 0.5364942261913939 1102 64-65 0.5360095683378532 1102 66-67 0.5288186194192264 1102 68-69 0.38463495684921867 1102 70-71 0.1535366575181456 1102 72-73 0.26703802382057873 1102 74-75 0.3402235535314695 1102 76-77 0.19202107604654728 1102 78-79 0.3052438440436198 1102 80-81 0.34287781601069867 1102 82-83 0.38918518430187987 1102 84-85 0.4321310239815297 1102 86-87 0.3617996608640901 1102 88-89 0.35171151605516826 1102 90-91 0.4810742535323449 1102 92-93 0.7700880801285059 1102 94-95 0.9698751277551843 1102 96-97 0.8300452567365788 1102 98-99 0.44008614717548156 1102 100-101 0.7959594934332088 1103 1 0.15337297828040164 1103 2 0.11562685148103924 1103 3 0.08463226440806437 1103 4 0.07204553326195651 1103 5 0.0570750281708996 1103 6 0.05248208956489009 1103 7 0.11417138645986569 1103 8 -0.023642178425454574 1103 9 0.12903626156969494 1103 10-11 0.11565319185920231 1103 12-13 0.15153874383503307 1103 14-15 0.1729184474718295 1103 16-17 0.08777625299760672 1103 18-19 0.15426707909842463 1103 20-21 0.2080531854001464 1103 22-23 0.13541176287078116 1103 24-25 -0.08753288433608475 1103 26-27 -0.03872752347886177 1103 28-29 0.05885135782647666 1103 30-31 0.22438584568273967 1103 32-33 0.1613803178852109 1103 34-35 0.18031657969485337 1103 36-37 0.17476405212816815 1103 38-39 0.09884798358356761 1103 40-41 0.30299346947720807 1103 42-43 0.30338085314084395 1103 44-45 0.4968768462240618 1103 46-47 0.41820092424321587 1103 48-49 0.3161238108267881 1103 50-51 0.2798203674238806 1103 52-53 0.15857017690444053 1103 54-55 0.27406462743152815 1103 56-57 0.23986497069066104 1103 58-59 0.23457200443975523 1103 60-61 0.20205459836716244 1103 62-63 -0.0937001887393265 1103 64-65 0.05630293472655268 1103 66-67 0.262580706629592 1103 68-69 0.2724814747667281 1103 70-71 0.2157837785887935 1103 72-73 0.3826714419387791 1103 74-75 0.2751860442748928 1103 76-77 0.2618304174076158 1103 78-79 0.2851195107854103 1103 80-81 0.40539324363768614 1103 82-83 0.42129342989896656 1103 84-85 0.4120724258296242 1103 86-87 0.41591306867932687 1103 88-89 0.5515864851999162 1103 90-91 0.5994712291808426 1103 92-93 0.6773304790037677 1103 94-95 0.7119681335398731 1103 96-97 0.7518942645389295 1103 98-99 0.8182776536631344 1103 100-101 1.2900111370791691 1104 1 0.19150131597770326 1104 2 0.16263198469306417 1104 3 0.11945711388808178 1104 4 0.14152326222244227 1104 5 0.08297870278146746 1104 6 0.05324208601454217 1104 7 0.09738489553544838 1104 8 0.2016792020683411 1104 9 0.22051228192502492 1104 10-11 0.20754619323496826 1104 12-13 0.19408328961233678 1104 14-15 0.17126947707286178 1104 16-17 0.2162225872629051 1104 18-19 0.1937684030825011 1104 20-21 0.1701485936162399 1104 22-23 0.1685372528928255 1104 24-25 0.08670508516051001 1104 26-27 0.17447459489567052 1104 28-29 0.08283331392050286 1104 30-31 0.17490887486955842 1104 32-33 0.06975174349800994 1104 34-35 0.07470342343514602 1104 36-37 0.034790642758203205 1104 38-39 0.12037890489897762 1104 40-41 0.17776417614446416 1104 42-43 0.18959172534309943 1104 44-45 0.1578731257548185 1104 46-47 0.13297666057067659 1104 48-49 0.23405020396869958 1104 50-51 0.2460291870809712 1104 52-53 0.3776382289466298 1104 54-55 0.2156188663995593 1104 56-57 0.2951006250642436 1104 58-59 0.40138382857917065 1104 60-61 0.35123441241766074 1104 62-63 0.31290114781889855 1104 64-65 0.35367465990000824 1104 66-67 0.5325098106104207 1104 68-69 0.3145170448195458 1104 70-71 0.3147622213172383 1104 72-73 0.3029323740531602 1104 74-75 0.6408109055894116 1104 76-77 0.6146624674379382 1104 78-79 0.6778333453095904 1104 80-81 0.7522835452693677 1104 82-83 0.5920258926572934 1104 84-85 0.5808845569262395 1104 86-87 0.7703589554527959 1104 88-89 0.620936623515572 1104 90-91 0.6194424010916677 1104 92-93 0.7916979252677656 1104 94-95 0.8703215300398206 1104 96-97 0.9773543305162384 1104 98-99 0.8379122821339671 1104 100-101 0.896493851320507 1105 1 0.11156426284779286 1105 2 0.09691269611835196 1105 3 0.09008798771331783 1105 4 0.08527572794551475 1105 5 0.09016003678493689 1105 6 0.06538492771647242 1105 7 0.016472699596256746 1105 8 0.08124653184562902 1105 9 0.1059564768698209 1105 10-11 0.09921313011786026 1105 12-13 0.07245406815933109 1105 14-15 -0.03631113518306961 1105 16-17 0.11861491285183945 1105 18-19 0.24098733767397107 1105 20-21 0.23728888177483043 1105 22-23 0.0585146628162434 1105 24-25 0.10606911775938954 1105 26-27 0.010062882643524063 1105 28-29 -0.02568805612981606 1105 30-31 0.034054782174585796 1105 32-33 0.011455294679670658 1105 34-35 0.0775543735153903 1105 36-37 0.1403786467790198 1105 38-39 -0.056177169021374596 1105 40-41 0.09772680524027066 1105 42-43 0.17900786238595856 1105 44-45 0.16466332176967313 1105 46-47 0.29887388653088465 1105 48-49 0.25108345272435173 1105 50-51 0.2884409749936623 1105 52-53 0.39626237573889256 1105 54-55 0.37980292148044015 1105 56-57 0.46325280543898373 1105 58-59 0.37500565826728405 1105 60-61 0.3562700606934115 1105 62-63 0.29896052378155247 1105 64-65 0.24788990468888272 1105 66-67 0.40748177567686383 1105 68-69 0.26956826430537717 1105 70-71 0.19490224061067352 1105 72-73 0.23939829032936544 1105 74-75 0.26319386143067547 1105 76-77 0.36168992005552525 1105 78-79 0.4318085637794127 1105 80-81 0.5549321371726066 1105 82-83 0.38717480206459953 1105 84-85 0.4213429855107904 1105 86-87 0.7152101879090118 1105 88-89 0.7070734502042875 1105 90-91 0.7020037604414213 1105 92-93 0.7043268859621143 1105 94-95 0.5617476029333517 1105 96-97 0.6964963129305666 1105 98-99 0.9751154158315529 1105 100-101 1.0634638357020094 1106 1 -0.20372825093861024 1106 2 -0.11439504233396036 1106 3 -0.2678420828990653 1106 4 -0.24154956964557073 1106 5 -0.24124386315796187 1106 6 -0.1570246566317195 1106 7 -0.20889427520155834 1106 8 -0.10967432012855483 1106 9 -0.3600193038776496 1106 10-11 -0.43294143294543375 1106 12-13 -0.5488953818301923 1106 14-15 -0.41287113944727594 1106 16-17 -0.24237192592497792 1106 18-19 -0.3747014221644207 1106 20-21 -0.26525857560116606 1106 22-23 -0.35607470846613154 1106 24-25 -0.3159189243912195 1106 26-27 -0.40899780823034604 1106 28-29 -0.4787271169923386 1106 30-31 -0.34727106109050254 1106 32-33 -0.45181425020653165 1106 34-35 -0.8007239617814008 1106 36-37 -0.604821861312125 1106 38-39 -0.5023993703642446 1106 40-41 -0.5194537496126159 1106 42-43 -0.7504691940099946 1106 44-45 -0.5183418693607749 1106 46-47 -0.6809174293023261 1106 48-49 -0.4793167774705722 1106 50-51 -0.6096943828745225 1106 52-53 -0.7476670462080435 1106 54-55 -0.6594862839314928 1106 56-57 -0.7669245024729321 1106 58-59 -0.7538723311950619 1106 60-61 -0.7476747376963075 1106 62-63 -0.841812300569238 1106 64-65 -1.1520679049540092 1106 66-67 -1.1550139330127251 1106 68-69 -0.9876409113293718 1106 70-71 -0.933599468686019 1106 72-73 -0.9232459880438739 1106 74-75 -0.9075887742108719 1106 76-77 -1.0344542697812074 1106 78-79 -0.7486563974452132 1106 80-81 -0.8741512479575952 1106 82-83 -1.159829501893885 1106 84-85 -1.0026251576075715 1106 86-87 -0.6596177079576009 1106 88-89 -0.9315333916803503 1106 90-91 -1.0763950025770583 1106 92-93 -1.0542771914151814 1106 94-95 -0.7298494240467939 1106 96-97 -0.6434057367799717 1106 98-99 -0.5711598170736742 1106 100-101 -0.21339163657702898 1107 1 0.04900590580594155 1107 2 0.07845276955369229 1107 3 -0.019204980603532817 1107 4 0.062967322219734 1107 5 0.058271966325875724 1107 6 0.07563147409629067 1107 7 0.056889959057492945 1107 8 0.024673653083688407 1107 9 0.11755559401569116 1107 10-11 0.02897760178263553 1107 12-13 0.09481556581407347 1107 14-15 0.09817738187511083 1107 16-17 0.019670364726849243 1107 18-19 0.15860472863079167 1107 20-21 -0.026508260821479723 1107 22-23 0.06978680420768768 1107 24-25 0.0654774973506207 1107 26-27 0.09216220412476162 1107 28-29 0.06635277802863726 1107 30-31 0.18795770505180087 1107 32-33 0.23439712042294758 1107 34-35 0.07124505405988657 1107 36-37 0.003916324612497135 1107 38-39 0.08624849346206531 1107 40-41 0.06887712122540535 1107 42-43 0.13536316219470734 1107 44-45 0.31486842118088276 1107 46-47 -0.13018158258429935 1107 48-49 0.0728473821069926 1107 50-51 0.07265872321410427 1107 52-53 -0.03842142558053041 1107 54-55 -0.034905580320177876 1107 56-57 0.06730906799443659 1107 58-59 0.11523194351317301 1107 60-61 0.059724601659809196 1107 62-63 0.12014436016907126 1107 64-65 0.18000942461246439 1107 66-67 0.21115526697468567 1107 68-69 0.1842324901799941 1107 70-71 0.08577227039309321 1107 72-73 0.10203967452803298 1107 74-75 0.22877305127526526 1107 76-77 0.052367134057529796 1107 78-79 0.27712258392770295 1107 80-81 0.36608590903090743 1107 82-83 0.5818942513817902 1107 84-85 0.5205733680430384 1107 86-87 0.7424430169800367 1107 88-89 0.6591234059871347 1107 90-91 0.8035895830548228 1107 92-93 0.7031179180888039 1107 94-95 0.6838018334980802 1107 96-97 0.6911042496058037 1107 98-99 0.8085485985614334 1107 100-101 1.1471727634135256 1108 1 0.04321084323071034 1108 2 0.006173519538947403 1108 3 0.05738910942877595 1108 4 0.05707133537921294 1108 5 0.12031373848606819 1108 6 0.0944929522654121 1108 7 0.13775519894922894 1108 8 0.06703519351128762 1108 9 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2316 94-95 1.395485821043156 2316 96-97 1.4190117413517314 2316 98-99 1.3814416848243276 2316 100-101 1.7260785730285164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 12.0 5 48.0 6 73.0 7 180.0 8 421.0 9 795.0 10 1272.0 11 2117.0 12 3093.0 13 4392.0 14 5671.0 15 7979.0 16 10402.0 17 14241.0 18 13419.0 19 8374.0 20 7197.0 21 7349.0 22 7765.0 23 8275.0 24 9675.0 25 11256.0 26 13666.0 27 16931.0 28 20993.0 29 25714.0 30 31870.0 31 39574.0 32 50310.0 33 65163.0 34 88208.0 35 125895.0 36 189741.0 37 282051.0 38 213383.0 39 16612.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55404019444421 19.937205290220334 15.66569219545985 21.84306231987561 2 15.65343146313653 20.775034256738053 40.35355560786827 23.217978672257143 3 19.273182054146734 25.015201786948012 30.431332013928134 25.280284144977124 4 13.03542687307869 17.660910818994836 37.33359506733644 31.97006724059004 5 13.331257673486949 37.702597309975516 34.36778903116021 14.598355985377335 6 30.927220821962347 37.11436727977346 17.443830068176084 14.514581830088114 7 27.642868042354902 33.483268065972304 20.771583661970535 18.10228022970226 8 26.010276638016506 35.82499503497753 19.429839056592055 18.73488927041391 9 25.905148517432785 17.244232330846092 20.66829585859616 36.18232329312496 10-11 24.678730457173135 27.486977838746807 27.009568882689745 20.824722821390313 12-13 25.61145109905721 24.721262498087686 28.08056749379529 21.58671890905982 14-15 22.52279884642783 26.078981813831977 26.764691674021545 24.633527665718645 16-17 21.40668479890317 29.416818812335954 27.39162640333772 21.784869985423157 18-19 21.207317101224348 27.576923342353442 30.39253199276449 20.823227563657724 20-21 22.08578278294282 26.611096605794177 30.716126858006493 20.586993753256507 22-23 21.704227096342798 26.417329672566915 30.693885469540838 21.184557761549453 24-25 21.57844773844185 26.66838174006593 30.820750803413482 20.93241971807874 26-27 21.46755539708191 26.85426623188656 30.68102131150998 20.99715705952155 28-29 21.18880545111989 26.938905711912973 30.85333245405448 21.018956382912645 30-31 21.38709308861204 26.717763585183302 30.851767816379283 21.043375509825378 32-33 21.267058884422283 26.84218698780214 30.639721345195714 21.251032782579856 34-35 21.633388875129107 26.861116627802744 30.42976007631182 21.075734420756326 36-37 21.648791252945475 26.67524972797728 30.49089078527343 21.18506823380382 38-39 21.46979234320689 27.005581528736172 30.480483819394337 21.044142308662604 40-41 21.5675020579019 26.909957254684098 30.454456052532 21.068084634882002 42-43 21.5246750268534 26.860266819855372 30.25106253573583 21.363995617555396 44-45 21.624225629024256 27.09548869240095 29.954919896359467 21.32536578221533 46-47 21.787055362109246 27.28481132531211 29.44979116233668 21.478342150241964 48-49 21.932172042054315 27.352979741941517 29.246857850064757 21.46799036593941 50-51 21.937731333624207 27.5937162368887 28.975142682093637 21.493409747393457 52-53 22.018973670428327 27.82659304375431 28.716923173657698 21.437510112159668 54-55 21.92300879594946 27.723918679449717 28.753921217553867 21.599151307046956 56-57 21.914420648972527 28.020439789805103 28.682455565924382 21.38268399529799 58-59 21.97058866379935 27.672811536948583 28.837157231334775 21.51944256791729 60-61 21.98577128077643 27.63462495485472 29.07513325046794 21.304470513900913 62-63 21.858751053389902 27.711381518461064 29.083069618433232 21.346797809715802 64-65 21.90226688740249 27.571440730667273 28.887152515521926 21.639139866408307 66-67 21.8277340404241 27.80036852352117 28.73452120697204 21.637376229082687 68-69 21.776090138736915 27.702985071193435 28.807175396799227 21.71374939327042 70-71 21.931596942926397 27.693476765611834 28.672295481331133 21.702630810130643 72-73 21.980825428276322 27.67001272119271 28.574528629584783 21.774633220946185 74-75 21.851504804378113 28.008631087711816 28.2997079263229 21.84015618158717 76-77 21.926382710833256 27.830120318405548 28.443252668651652 21.80024430210954 78-79 21.92385227467041 27.804777616835224 28.43063882777928 21.840731280715087 80-81 21.79295971315589 28.042408576491635 28.426038034755923 21.73859367559655 82-83 21.87652760881559 27.97354387608823 28.372701469916723 21.777227045179455 84-85 21.905661252668946 27.78821265995946 28.35836746555818 21.947758621813414 86-87 21.89517399815815 27.804777616835224 28.39701469876691 21.903033686239716 88-89 21.875620627808882 27.749184701136322 28.351121788358917 22.02407288269588 90-91 21.917608620047723 27.785282559173485 28.45308242013799 21.844026400640807 92-93 21.904268583513662 27.626513895555966 28.512114998550707 21.95710252237966 94-95 21.684572697513964 27.720429744740333 28.44731670248895 22.147680855256752 96-97 21.795720188969906 27.73043646956614 28.320066435451256 22.153776906012702 98-99 21.84534752428366 27.672851564372074 28.09754489146875 22.384256019875508 100-101 21.70104371613406 27.56554812639936 28.433911974149247 22.299496183317338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 666.0 1 479.0 2 352.0 3 453.0 4 386.0 5 323.0 6 546.0 7 1226.0 8 2014.5 9 2403.0 10 2195.0 11 1867.5 12 1869.0 13 1961.5 14 2162.5 15 2334.0 16 2507.5 17 2680.5 18 2789.0 19 2943.5 20 3191.5 21 3605.5 22 4245.5 23 5050.5 24 6050.0 25 7355.5 26 8938.0 27 10918.5 28 12760.0 29 14896.5 30 17531.5 31 19617.5 32 21658.5 33 24302.5 34 27396.0 35 30225.5 36 33474.0 37 36620.0 38 38577.0 39 39740.5 40 40793.5 41 42899.0 42 44636.5 43 46355.5 44 48394.5 45 49626.5 46 49955.5 47 49795.5 48 51194.0 49 51024.5 50 48341.5 51 45406.5 52 42726.0 53 40499.0 54 38220.5 55 34948.5 56 30916.0 57 27397.5 58 24397.0 59 21406.0 60 17851.0 61 14339.0 62 11203.5 63 8561.5 64 6337.0 65 4725.0 66 3643.5 67 2910.0 68 2174.0 69 1434.0 70 993.0 71 756.0 72 618.5 73 479.0 74 379.0 75 313.5 76 194.5 77 101.0 78 64.0 79 46.5 80 30.0 81 21.5 82 15.0 83 8.5 84 4.5 85 2.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0391067406985384 2 0.0 3 0.0 4 0.0 5 0.009201586046714918 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005635971453612888 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.683795930291851E-4 22-23 0.0018786571512042958 24-25 0.0 26-27 0.0011885381977006768 28-29 0.0018403172093429839 30-31 0.0 32-33 3.833994186131216E-5 34-35 0.0 36-37 3.067195348904973E-4 38-39 0.0 40-41 2.683795930291851E-4 42-43 0.02181542691908662 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1501982558393649E-4 84-85 2.683795930291851E-4 86-87 0.0 88-89 0.0 90-91 0.010696843779306093 92-93 0.0028371556977371 94-95 0.0 96-97 0.0 98-99 4.2173936047443375E-4 100-101 0.013533999477043193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1304123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.09108737630913 #Duplication Level Percentage of deduplicated Percentage of total 1 82.57962142103918 54.57776974840429 2 10.762431216032516 14.226015637606437 3 2.968925684090181 5.886585804029176 4 1.2152479445917097 3.2126823235956308 5 0.6585419903172244 2.17618781115121 6 0.3759911806225131 1.4909799582746484 7 0.27307436516876266 1.2633447210019186 8 0.1853745289143171 0.9801283350254619 9 0.13342982373616533 0.7936669925237185 >10 0.7788126748372651 9.285168050675919 >50 0.04849704088992192 2.1409793716247476 >100 0.01877715946372164 2.079280180107675 >500 6.954383435261051E-4 0.2693389392751622 >1k 3.4771917176305253E-4 0.547104295876908 >5k 2.3181278117536837E-4 1.070767830827138 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7333 0.5622935873380042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6645 0.5095378273368386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4265 0.32703970407699273 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.1346498758169283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.5335976744524865E-4 0.0 0.0 0.0 0.0 14-15 2.683795930291851E-4 0.0 0.0 3.833994186131216E-5 0.0 16-17 3.067195348904973E-4 0.0 0.0 1.5335976744524865E-4 0.0 18-19 3.067195348904973E-4 0.0 0.0 1.5335976744524865E-4 0.0 20-21 3.067195348904973E-4 0.0 0.0 1.916997093065608E-4 0.0 22-23 3.067195348904973E-4 0.0 0.0 3.4505947675180946E-4 0.0 24-25 3.067195348904973E-4 0.0 0.0 7.28458895364931E-4 0.0 26-27 3.067195348904973E-4 0.0 0.0 8.434787209488676E-4 0.0 28-29 3.067195348904973E-4 0.0 0.0 0.0016869574418977352 0.0 30-31 3.067195348904973E-4 0.0 0.0 0.003067195348904973 0.0 32-33 3.067195348904973E-4 0.0 0.0 0.0052142320931384545 0.0 34-35 3.067195348904973E-4 0.0 0.0 0.00728458895364931 0.0 36-37 3.067195348904973E-4 0.0 0.0 0.011080243197919216 0.0 38-39 3.067195348904973E-4 0.0 0.0 0.016562854884086854 0.0 40-41 3.067195348904973E-4 0.0 0.0 0.02568776104707915 0.0 42-43 3.067195348904973E-4 0.0 0.0 0.03285733017514453 0.0 44-45 3.067195348904973E-4 0.0 0.0 0.04209725616372076 0.0 46-47 3.067195348904973E-4 0.0 0.0 0.059886989187369594 0.0 48-49 3.067195348904973E-4 0.0 0.0 0.0737660481411646 0.0 50-51 3.067195348904973E-4 0.0 0.0 0.0892553846531347 0.0 52-53 3.067195348904973E-4 0.0 0.0 0.10643167860700256 0.0 54-55 3.067195348904973E-4 0.0 0.0 0.12870718482842491 0.0 56-57 3.067195348904973E-4 0.0 0.0 0.1755969337248097 0.0 58-59 3.067195348904973E-4 0.0 0.0 0.2203779858188223 0.0 60-61 3.067195348904973E-4 0.0 0.0 0.25902464721502494 0.0 62-63 3.067195348904973E-4 0.0 0.0 0.305454316809074 0.0 64-65 3.067195348904973E-4 0.0 0.0 0.3533409041938529 0.0 66-67 3.8339941861312163E-4 0.0 0.0 0.42315793832330234 0.0 68-69 3.8339941861312163E-4 0.0 0.0 0.507735850069357 0.0 70-71 4.217393604744338E-4 0.0 0.0 0.562025207744975 0.0 72-73 4.600793023357459E-4 0.0 0.0 0.624787692571943 0.0 74-75 4.600793023357459E-4 0.0 0.0 0.722669564143873 0.0 76-77 4.600793023357459E-4 0.0 0.0 0.8351589535649628 0.0 78-79 4.600793023357459E-4 0.0 0.0 0.9599171243816726 0.0 80-81 4.600793023357459E-4 0.0 0.0 1.0765855674656455 0.0 82-83 4.600793023357459E-4 0.0 0.0 1.2206287290385953 0.0 84-85 4.984192441970581E-4 0.0 0.0 1.3941169659610329 0.0 86-87 5.367591860583703E-4 0.0 0.0 1.5804107434651486 0.0 88-89 5.367591860583703E-4 0.0 0.0 1.828086767889225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4220 0.0 46.391613 1 GTATCAA 6815 0.0 36.11758 1 ATCAACG 8735 0.0 27.788351 3 TCAACGC 8840 0.0 27.45829 4 TATCAAC 8870 0.0 27.418972 2 CAACGCA 8985 0.0 27.015163 5 AACGCAG 9270 0.0 26.182594 6 CGTAGAC 95 0.0012264588 25.000647 3 ATAACGC 95 0.0012264588 25.000647 3 GTACCTG 1050 0.0 23.53254 1 ACGCAGA 10270 0.0 23.495325 7 GTACATG 5445 0.0 23.213385 1 CGCAGAG 10370 0.0 23.13134 8 TACATGG 5525 0.0 22.611444 2 ACATGGG 5465 0.0 22.25126 3 GAGTACT 6570 0.0 22.014565 12-13 TACCTGG 1130 0.0 21.438608 2 GCAGAGT 10990 0.0 21.4374 9 AGTACTT 6820 0.0 21.03346 12-13 GTACTTT 6940 0.0 20.8743 14-15 >>END_MODULE