FastQCFastQC Report
Thu 26 May 2016
SRR937872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035064
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50710.4899213961648748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.4099263427189043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.2538973435459063No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15650.15119838000355534No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.10859231892907106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.046.029761
GTATCAA47250.038.3351971
ATCAACG58500.030.7885653
TCAACGC59700.030.2551674
TATCAAC60250.030.2097972
CAACGCA60550.029.9060465
AACGCAG61950.029.2174576
TCTCGCG350.00837902627.1521950-51
ACGCAGA70100.025.8205627
CGCAGAG70600.025.5704068
GCAGAGT74000.023.7512599
GTACATG38900.023.7097321
GTGTAGC6150.023.1910651
TACATGG39900.022.6301172
ACATGGG40500.021.9428313
GTACCTG7600.021.8941961
GAGTACT45000.021.84904112-13
CGTTAGT550.002881295721.58682490-91
GTGGTAT16100.021.5561661
TGGTATC15150.021.3305642
Produced by FastQC (version 0.11.5)