##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937872_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035064 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.546718850235347 33.0 31.0 34.0 30.0 34.0 2 31.642074306516314 33.0 31.0 34.0 30.0 34.0 3 31.67682288244978 33.0 31.0 34.0 30.0 34.0 4 35.28692911742655 37.0 35.0 37.0 33.0 37.0 5 35.28303274000448 37.0 35.0 37.0 33.0 37.0 6 35.33133120270824 37.0 35.0 37.0 33.0 37.0 7 35.242584033451074 37.0 35.0 37.0 33.0 37.0 8 35.243008161814146 37.0 35.0 37.0 33.0 37.0 9 36.784399805229434 39.0 37.0 39.0 33.0 39.0 10-11 36.69176108916936 39.0 37.0 39.0 32.0 39.0 12-13 36.57789518329301 39.0 37.0 39.0 32.0 39.0 14-15 37.660975553202505 40.0 37.5 41.0 32.0 41.0 16-17 37.567394383342474 40.0 37.0 41.0 32.0 41.0 18-19 37.49812958425759 40.0 37.0 41.0 32.0 41.0 20-21 37.509967016532315 40.0 37.0 41.0 32.0 41.0 22-23 37.50152164503837 40.0 37.0 41.0 32.0 41.0 24-25 37.417923432753916 40.0 37.0 41.0 32.0 41.0 26-27 37.30536807385823 40.0 37.0 41.0 31.5 41.0 28-29 37.14061787483672 40.0 37.0 41.0 31.0 41.0 30-31 36.88428734841517 39.0 36.0 41.0 30.5 41.0 32-33 36.73576609755532 39.0 36.0 41.0 30.0 41.0 34-35 36.70731809820455 39.0 36.0 41.0 30.0 41.0 36-37 36.531115950318046 39.0 36.0 41.0 30.0 41.0 38-39 36.33930897026657 39.0 35.0 41.0 30.0 41.0 40-41 36.09225323265035 39.0 35.0 41.0 29.5 41.0 42-43 35.82312011624402 39.0 35.0 40.5 28.5 41.0 44-45 35.52803932896903 39.0 35.0 40.0 27.0 41.0 46-47 35.130925237473235 38.5 35.0 40.0 25.0 41.0 48-49 34.756657076277406 38.0 34.0 40.0 24.0 41.0 50-51 33.87537002542838 37.5 33.5 39.5 22.5 40.5 52-53 33.98367685476454 38.0 33.5 39.5 23.0 40.5 54-55 34.227695582108936 38.0 34.0 40.0 22.5 41.0 56-57 33.97504067381341 38.0 33.5 40.0 21.0 41.0 58-59 33.67817448969339 38.0 33.0 40.0 19.5 41.0 60-61 33.31207393938925 37.5 33.0 40.0 18.0 41.0 62-63 32.92211447794533 37.0 32.0 40.0 14.0 41.0 64-65 32.46812032879126 36.5 31.5 39.0 10.0 41.0 66-67 31.935783681009095 36.0 31.0 39.0 8.0 40.5 68-69 31.467457567841215 35.0 31.0 39.0 6.5 40.0 70-71 30.98192044163453 35.0 30.0 38.0 2.0 40.0 72-73 30.49621714212841 35.0 29.5 37.5 2.0 39.5 74-75 29.93748405895674 34.5 29.0 37.0 2.0 39.0 76-77 29.36921436742076 34.0 29.0 36.0 2.0 39.0 78-79 28.811815018201774 34.0 28.0 36.0 2.0 38.5 80-81 28.236770383280646 34.0 26.5 35.0 2.0 37.0 82-83 27.65973408407596 33.5 26.0 35.0 2.0 37.0 84-85 27.081950971147677 33.0 25.5 35.0 2.0 36.0 86-87 26.468790818731982 32.5 24.5 35.0 2.0 36.0 88-89 25.892189758314462 32.0 22.5 35.0 2.0 35.5 90-91 25.282396547459868 32.0 19.5 34.0 2.0 35.0 92-93 24.580145768764055 31.0 16.5 34.0 2.0 35.0 94-95 23.771828601902875 31.0 4.5 34.0 2.0 35.0 96-97 22.84865718448328 31.0 2.0 34.0 2.0 35.0 98-99 21.638818952258028 30.0 2.0 34.0 2.0 35.0 100-101 18.57197429337703 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13391844997642366 1101 2 0.14774982633924694 1101 3 0.1413413094098459 1101 4 0.1324440416990882 1101 5 0.004753873396204256 1101 6 0.08240151626261394 1101 7 0.023957852825475356 1101 8 0.12113473957973753 1101 9 0.3038872620987192 1101 10-11 0.3406750221457884 1101 12-13 0.29378308958019517 1101 14-15 0.3425536821283899 1101 16-17 0.33821805986239184 1101 18-19 0.487893440667861 1101 20-21 0.39010572964394186 1101 22-23 0.3912042540834477 1101 24-25 0.26630241351009687 1101 26-27 0.46830420046533305 1101 28-29 0.46504191539808204 1101 30-31 0.4407439115432794 1101 32-33 0.5128603801629197 1101 34-35 0.5664199020591028 1101 36-37 0.5316938358869905 1101 38-39 0.5126613403121496 1101 40-41 0.5147797320803349 1101 42-43 0.342469195240902 1101 44-45 0.5190868806606446 1101 46-47 0.6392744224786497 1101 48-49 0.6947380521264108 1101 50-51 0.5768422894079848 1101 52-53 0.9793166289598929 1101 54-55 0.936172865104048 1101 56-57 0.980754654330454 1101 58-59 0.9651661709984083 1101 60-61 1.029924849057096 1101 62-63 0.9575286636861193 1101 64-65 0.8903528798928733 1101 66-67 0.86151707575317 1101 68-69 0.9371770554239909 1101 70-71 0.9546772544049595 1101 72-73 0.9045961146234802 1101 74-75 1.0037438142330082 1101 76-77 0.8272346784269224 1101 78-79 0.9202771603346527 1101 80-81 1.1904232221438669 1101 82-83 1.250875236011403 1101 84-85 1.4141193863141197 1101 86-87 1.5738235631726987 1101 88-89 1.5929024132853726 1101 90-91 1.3914551366975338 1101 92-93 1.5449210920730785 1101 94-95 1.8568635065822114 1101 96-97 2.1533124841552116 1101 98-99 2.27989549212419 1101 100-101 2.75214921129761 1102 1 0.12190696629967945 1102 2 0.11030856424030233 1102 3 -0.015545425110008892 1102 4 0.11327447142021185 1102 5 -0.08921294959029069 1102 6 -0.03753907006819901 1102 7 0.11425564244014907 1102 8 0.17629494549859004 1102 9 0.23614428722246572 1102 10-11 0.2558244966644452 1102 12-13 0.19592360621082605 1102 14-15 0.32870481691328735 1102 16-17 0.3277579780422073 1102 18-19 0.175590652054197 1102 20-21 0.16640170462736847 1102 22-23 0.12848683706137365 1102 24-25 0.07659710908414041 1102 26-27 0.18871044328214026 1102 28-29 0.2958624361001725 1102 30-31 0.31741487885901876 1102 32-33 0.311167016441928 1102 34-35 0.3557002309037216 1102 36-37 0.47220298420342743 1102 38-39 0.508342996560863 1102 40-41 0.3957378668119773 1102 42-43 0.4054128681974376 1102 44-45 0.4072251782102896 1102 46-47 0.40675086496555224 1102 48-49 0.448663068489779 1102 50-51 0.10501727055051191 1102 52-53 0.24702276520419275 1102 54-55 0.4422369151156076 1102 56-57 0.5056888132805568 1102 58-59 0.3361338911892773 1102 60-61 0.3433713878771911 1102 62-63 0.45683756016518373 1102 64-65 0.3053132741504925 1102 66-67 0.5458971250555891 1102 68-69 0.6088882216750839 1102 70-71 0.4738714287106731 1102 72-73 0.49652349971424314 1102 74-75 0.3837648051753568 1102 76-77 0.43791184865375854 1102 78-79 0.6116755925525794 1102 80-81 0.8822655379692463 1102 82-83 0.9732290729251254 1102 84-85 1.104712120913515 1102 86-87 1.2326162671687264 1102 88-89 1.0534552489163183 1102 90-91 0.9943147986988699 1102 92-93 1.1017516754851115 1102 94-95 1.3197929900931342 1102 96-97 1.1792053575482804 1102 98-99 1.352211951139612 1102 100-101 1.9113091219681415 1103 1 -0.06790265606396417 1103 2 -0.13928435906266046 1103 3 -0.022794660700871106 1103 4 -0.07499841489643444 1103 5 -0.14720924407352953 1103 6 -0.13891855230106387 1103 7 -0.12872387669641228 1103 8 -0.18405451048170107 1103 9 -0.10267606131092322 1103 10-11 -0.16121172335044776 1103 12-13 -0.06424281156243694 1103 14-15 -0.12175214174010307 1103 16-17 -0.0918432554776416 1103 18-19 -0.15252366540985207 1103 20-21 -0.15504550552885377 1103 22-23 -0.3757756776639667 1103 24-25 -0.348159109636363 1103 26-27 -0.31708433222839716 1103 28-29 -0.33373271106364655 1103 30-31 -0.3229711572226037 1103 32-33 -0.4432622878476451 1103 34-35 -0.28934128231311007 1103 36-37 -0.3821909310929428 1103 38-39 -0.3158485818226566 1103 40-41 -0.1804680957514222 1103 42-43 -0.03458437091029509 1103 44-45 -0.05644671211963015 1103 46-47 -0.10887227392797882 1103 48-49 0.11805654658749631 1103 50-51 -0.027620882502056077 1103 52-53 0.03430444988550363 1103 54-55 0.007024591483954623 1103 56-57 -0.005181081220314354 1103 58-59 -0.031816280557499965 1103 60-61 0.29180294632170245 1103 62-63 0.4113477289927161 1103 64-65 0.26867568291540067 1103 66-67 0.29444817298977455 1103 68-69 0.24496544128443887 1103 70-71 0.21686643913803394 1103 72-73 0.14196985199721723 1103 74-75 0.426374805388793 1103 76-77 0.3946706644030975 1103 78-79 0.5045880860376322 1103 80-81 0.570245466764387 1103 82-83 0.5083934424054917 1103 84-85 0.7290612285118918 1103 86-87 0.8632095698361901 1103 88-89 0.756478425952853 1103 90-91 0.9415962148644788 1103 92-93 1.0449444236332148 1103 94-95 1.3372058894500043 1103 96-97 0.9708254642798337 1103 98-99 1.1965022744531773 1103 100-101 1.9031075162442157 1104 1 0.09700461485654088 1104 2 0.13794808315196505 1104 3 -0.05242198902666573 1104 4 0.08394982559764941 1104 5 0.07987839961412391 1104 6 0.07717130724839905 1104 7 0.2266549681029204 1104 8 0.20166996322357278 1104 9 0.31522064001485006 1104 10-11 0.11324721930816395 1104 12-13 0.18063213583196358 1104 14-15 0.11623523585316775 1104 16-17 0.21248648855616636 1104 18-19 0.2085358499796257 1104 20-21 0.10389946662414218 1104 22-23 0.01361962047851506 1104 24-25 -0.05869609440443213 1104 26-27 0.1385727255154734 1104 28-29 0.04321443068027264 1104 30-31 0.12222518928618342 1104 32-33 0.0384561177600915 1104 34-35 0.2583603394450833 1104 36-37 0.1915298071555327 1104 38-39 0.3701396727142807 1104 40-41 0.4370463932820101 1104 42-43 0.429859383143004 1104 44-45 0.36306561466746956 1104 46-47 0.34308880416921994 1104 48-49 0.3143979141642319 1104 50-51 0.13166025138460924 1104 52-53 0.2553946876811892 1104 54-55 0.3003242351980546 1104 56-57 0.4538985539300029 1104 58-59 0.45776560681032663 1104 60-61 0.32673031203144376 1104 62-63 0.41157045358466604 1104 64-65 0.2289644206526873 1104 66-67 0.42740649172574763 1104 68-69 0.42506150113400665 1104 70-71 0.528485869095281 1104 72-73 0.35247415335442156 1104 74-75 0.5277117405074492 1104 76-77 0.48453572231149167 1104 78-79 0.6882249711396504 1104 80-81 0.8402021777703226 1104 82-83 0.8883858334136647 1104 84-85 1.0720949247412541 1104 86-87 1.1765344128272908 1104 88-89 1.286851413444289 1104 90-91 1.2886727388478612 1104 92-93 1.0963471662487798 1104 94-95 1.1941718994371726 1104 96-97 1.341083890907779 1104 98-99 1.3984157963514328 1104 100-101 2.0193917870038725 1105 1 0.08573382307118393 1105 2 0.018241862369034578 1105 3 0.0691975521146162 1105 4 -0.03565183000290517 1105 5 -0.0779754244903259 1105 6 -2.1093233312541315E-4 1105 7 -0.07743215115745272 1105 8 -0.09866882796732312 1105 9 0.08760048127155073 1105 10-11 -0.05075125537504732 1105 12-13 0.08173994831944498 1105 14-15 0.16545161992686985 1105 16-17 0.1682965750471439 1105 18-19 0.14997534322560568 1105 20-21 0.17395213187940328 1105 22-23 0.15692331069504206 1105 24-25 0.24144869500129573 1105 26-27 0.2458432538855675 1105 28-29 0.2651291126107864 1105 30-31 0.27158320356222276 1105 32-33 0.3134369623086002 1105 34-35 0.29589746008483786 1105 36-37 0.3819765986062791 1105 38-39 0.4015477163130683 1105 40-41 0.5021669191158367 1105 42-43 0.3244750326796648 1105 44-45 0.5420929982864209 1105 46-47 0.5010216083582364 1105 48-49 0.42145708737845666 1105 50-51 0.3844216621157628 1105 52-53 0.33064224018848165 1105 54-55 0.441319259675204 1105 56-57 0.45637964098072814 1105 58-59 0.39992971452225134 1105 60-61 0.5795104110079521 1105 62-63 0.631666146300077 1105 64-65 0.5738722297584928 1105 66-67 0.8539897198264583 1105 68-69 0.6433123970573895 1105 70-71 0.8361317642221842 1105 72-73 0.7714067697710689 1105 74-75 0.7210269551419479 1105 76-77 0.6769721444558137 1105 78-79 1.0185781967752945 1105 80-81 1.2074913854866125 1105 82-83 1.222458242355188 1105 84-85 1.4732256525772165 1105 86-87 1.6852965301520904 1105 88-89 1.582392887555283 1105 90-91 1.6667501811568037 1105 92-93 1.8575752420447458 1105 94-95 1.6770450124805905 1105 96-97 1.5552910065016974 1105 98-99 1.769301156424973 1105 100-101 2.601495923246521 1106 1 -0.19722836753977546 1106 2 -0.2173100520984974 1106 3 -0.10905687718621238 1106 4 -0.08169007797754801 1106 5 -0.09154509753717832 1106 6 -0.1302722515655148 1106 7 -0.08644715409514703 1106 8 0.06612681596737247 1106 9 0.046163244526354674 1106 10-11 -0.3445870588555948 1106 12-13 -0.5860347676343096 1106 14-15 -0.5667620788392895 1106 16-17 -0.5885035705963801 1106 18-19 -0.060729455562906764 1106 20-21 0.015102219182764998 1106 22-23 -0.11378661871749784 1106 24-25 -0.27610796943107374 1106 26-27 -0.06851752828634261 1106 28-29 0.1427173883073536 1106 30-31 0.07172222692311436 1106 32-33 -0.03237040758456544 1106 34-35 0.020956420885205773 1106 36-37 -0.23442319285867796 1106 38-39 -0.6378331332189688 1106 40-41 -0.4031697573983237 1106 42-43 -0.1542229473440031 1106 44-45 -0.16648010896844312 1106 46-47 0.01321677067628002 1106 48-49 0.08486663530428018 1106 50-51 0.22524653405704242 1106 52-53 0.29407661371446636 1106 54-55 0.37567458021507605 1106 56-57 0.20236906527506449 1106 58-59 -0.12034866320752968 1106 60-61 0.18300502799031193 1106 62-63 -0.11205670069974616 1106 64-65 0.29399172364725956 1106 66-67 0.483099391052761 1106 68-69 0.29874051453213113 1106 70-71 0.5027926382488168 1106 72-73 0.3678145054280755 1106 74-75 0.7074308014549295 1106 76-77 0.9547747478337527 1106 78-79 0.8492394680115289 1106 80-81 0.9959938225330269 1106 82-83 0.9724354855550423 1106 84-85 0.8789024398135474 1106 86-87 1.237748058167785 1106 88-89 1.3242678823832854 1106 90-91 1.4504248664923232 1106 92-93 1.5489069699892681 1106 94-95 1.3798800960187307 1106 96-97 1.5855385177352836 1106 98-99 1.8281452764815747 1106 100-101 2.3258829378025965 1107 1 -0.12559303606126093 1107 2 -0.05741721937875255 1107 3 -0.1767446307014673 1107 4 0.0036334379336082634 1107 5 -0.1307829358307444 1107 6 -0.012069739129806578 1107 7 -0.05197188968985955 1107 8 0.011389066667611303 1107 9 0.1759087222357465 1107 10-11 0.09272851318723241 1107 12-13 0.04742034669931883 1107 14-15 0.22750484637780488 1107 16-17 0.03272473130809317 1107 18-19 0.1618403108986044 1107 20-21 -0.07704196952158071 1107 22-23 -0.057971575953629895 1107 24-25 -0.06450130332647319 1107 26-27 -0.04708969289678322 1107 28-29 -0.05529102394944374 1107 30-31 -0.12136716969887118 1107 32-33 -0.027843901481460875 1107 34-35 0.10972633910458285 1107 36-37 -0.12703764357952707 1107 38-39 0.11571209905486768 1107 40-41 0.2912845232038421 1107 42-43 0.2233908944718408 1107 44-45 0.1923972575642594 1107 46-47 0.1497698063019115 1107 48-49 0.07160468437265877 1107 50-51 0.051639287150742064 1107 52-53 0.1663105456482512 1107 54-55 0.32043846559105305 1107 56-57 0.5929460261833697 1107 58-59 0.37652718574656063 1107 60-61 0.4365736796872923 1107 62-63 0.40710132419977896 1107 64-65 0.5514255169380675 1107 66-67 0.7464789074885161 1107 68-69 0.7795628173171671 1107 70-71 0.6769919281719048 1107 72-73 0.8324689752591752 1107 74-75 0.9757096612100575 1107 76-77 0.9130226002726509 1107 78-79 1.0947424703815756 1107 80-81 1.2069606699976418 1107 82-83 1.3693291332707105 1107 84-85 1.5341769622915074 1107 86-87 1.5273695066122635 1107 88-89 1.5181226257532465 1107 90-91 1.6845507131044677 1107 92-93 2.069944169400973 1107 94-95 2.171333880129122 1107 96-97 2.022328557923011 1107 98-99 2.2001937265841534 1107 100-101 2.6087305523001554 1108 1 -0.022128417514789334 1108 2 0.15868736585919052 1108 3 0.11451675266552996 1108 4 -0.11466942163905003 1108 5 0.025696281773171847 1108 6 0.041735494561422115 1108 7 0.11231179976125816 1108 8 0.008297397343348223 1108 9 0.2741888150744103 1108 10-11 0.2869721615342087 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56-57 0.3156190336974447 2316 58-59 0.22778930993045066 2316 60-61 0.38556702537989196 2316 62-63 0.4327266768422504 2316 64-65 0.48245536728331473 2316 66-67 0.6664021993555735 2316 68-69 0.7061896745674936 2316 70-71 0.48686278266378835 2316 72-73 0.6564276164549767 2316 74-75 0.5783151852703519 2316 76-77 0.6568978163361336 2316 78-79 0.24827740325635972 2316 80-81 -0.021852794451518776 2316 82-83 0.4780773532658742 2316 84-85 0.28109497907505343 2316 86-87 -0.19413586250924197 2316 88-89 -0.04160829125151011 2316 90-91 -0.2504119403789673 2316 92-93 -0.10436868156224577 2316 94-95 0.17375088040675735 2316 96-97 0.07896405771254322 2316 98-99 -0.5304154156998067 2316 100-101 -1.132685122489601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5394.0 3 1229.0 4 1234.0 5 1249.0 6 1402.0 7 1737.0 8 1997.0 9 2553.0 10 3177.0 11 3990.0 12 5078.0 13 6155.0 14 6977.0 15 8302.0 16 10088.0 17 12575.0 18 11589.0 19 8645.0 20 8707.0 21 9159.0 22 9978.0 23 10930.0 24 12405.0 25 13918.0 26 15738.0 27 18278.0 28 21442.0 29 24956.0 30 29603.0 31 36449.0 32 45068.0 33 57372.0 34 75724.0 35 102577.0 36 141948.0 37 180354.0 38 117358.0 39 9728.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58734735068553 19.145231699289695 15.019325643145965 22.248095306878803 2 14.962404557962868 19.979942637882253 41.60206968722143 23.45558311693345 3 19.06999414712802 24.898834464500975 30.072908954894125 25.958262433476875 4 12.378130432170659 16.91998224885325 37.995399429870375 32.706487889105716 5 13.318476028060926 37.36589667067168 34.611642959575214 14.703984341692184 6 30.80949125494578 36.386048970754274 18.18159810515756 14.622861669142381 7 27.698748659000866 33.12635794316875 21.021347544374 18.153545853456386 8 25.723312947592248 35.37329532470651 19.96847246375689 18.93491926394435 9 25.90986995632934 17.252718522750378 20.92405565830372 35.913355862616555 10-11 24.50861152373519 27.12547666957817 27.49141559002446 20.87449621666218 12-13 25.39755937304658 24.491051857633483 28.22883383390896 21.882554935410976 14-15 22.401164710473388 25.864406320113332 26.820441114240978 24.9139878551723 16-17 21.421430677345036 29.225230912529916 27.39656924422773 21.95676916589732 18-19 21.24521484545185 27.454104147424392 30.270156707155323 21.030524299968437 20-21 22.05865837412479 26.564242673028073 30.41235516450833 20.964743788338808 22-23 21.730428952152263 26.448781597732395 30.293592949315336 21.527196500800002 24-25 21.508674004129443 26.612091564176982 30.50649793891042 21.37273649278316 26-27 21.393045905698184 26.87414337427385 30.298564475625227 21.434246244402733 28-29 21.131612495273288 26.924661649694077 30.516527395081503 21.42719845995113 30-31 21.369047993713362 26.67197008908491 30.54103139784238 21.417950519359348 32-33 21.18206017834854 26.843691808923626 30.3081639629049 21.66608404982294 34-35 21.561101965720926 26.933572189406412 30.058535876827737 21.44678996804493 36-37 21.56997439580341 26.836044551011607 30.090135098565753 21.503845954619237 38-39 21.387844525089175 27.020486956706947 30.16795499772249 21.423713520481392 40-41 21.52489679277092 26.915874189068774 30.09493274165545 21.46429627650486 42-43 21.4272782282464 26.881035943361976 29.94089313808544 21.750792690306184 44-45 21.509110624866874 27.10439943457971 29.705526402416595 21.680963538136826 46-47 21.56596000135556 27.22948145064074 29.33722894890999 21.86732959909371 48-49 21.71632978252102 27.31867592353641 29.16896853752078 21.7960257564218 50-51 21.78251472792554 27.480217520131067 28.92904451493708 21.80822323700631 52-53 21.791770954538478 27.659443626927597 28.75357653531358 21.79520888322034 54-55 21.711315589957405 27.526020056915396 28.803082223283027 21.959582129844176 56-57 21.726142074979286 27.815837263511682 28.704870710684666 21.75314995082437 58-59 21.7745120053271 27.54701860612771 28.841094919899962 21.83737446864523 60-61 21.785021451582924 27.454492828821014 29.105809739173534 21.654675980422528 62-63 21.673714120057245 27.514106602795135 29.073177200419508 21.73900207672811 64-65 21.634302867849314 27.42329177079083 28.994943474070965 21.947461887288892 66-67 21.681496242308313 27.656484976735562 28.74194892714478 21.920069853811345 68-69 21.539374450047095 27.561292221136974 28.872660673369836 22.026672655446095 70-71 21.66778070175014 27.548337102982824 28.76233686968887 22.021545325578167 72-73 21.673945433932964 27.445002628488556 28.73242954634127 22.14862239123721 74-75 21.54824617881047 27.774894733937543 28.577376001951865 22.099483085300122 76-77 21.674708249565164 27.631369386282078 28.526359561625213 22.167562802527545 78-79 21.698832465956404 27.641352749797054 28.509681688560452 22.15013309568609 80-81 21.58998331423692 27.759601090698037 28.542239377529548 22.108176217535494 82-83 21.686922514726025 27.72369764819198 28.51984685901252 22.069532978069475 84-85 21.725320023095772 27.494616874917423 28.518439109099454 22.26162399288735 86-87 21.699921396737384 27.571594794373087 28.525288566903768 22.20319524198576 88-89 21.786990984449687 27.565607793307922 28.333030798087975 22.314370424154415 90-91 21.74786201502059 27.541815830285522 28.485539922283255 22.224782232410632 92-93 21.745607810831665 27.356061361416494 28.656705003838233 22.241625823913605 94-95 21.616254215827116 27.490048518313436 28.453686888438856 22.44001037742059 96-97 21.732528313723364 27.456409427306934 28.318031474375697 22.493030784594005 98-99 21.741733804995828 27.38856586333018 28.231594803967695 22.638105527706298 100-101 22.156612220205687 26.68888082274652 28.752571082879612 22.40193587416818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1572.0 1 941.0 2 349.0 3 397.5 4 327.0 5 281.0 6 450.5 7 870.0 8 1457.0 9 1785.5 10 1605.0 11 1333.0 12 1311.5 13 1428.5 14 1575.5 15 1723.5 16 1778.5 17 1843.5 18 1981.5 19 2158.0 20 2375.5 21 2721.0 22 3224.5 23 3846.5 24 4691.0 25 5809.5 26 7220.5 27 8864.5 28 10207.0 29 11671.5 30 13734.5 31 15549.5 32 17338.0 33 19272.0 34 21621.5 35 23958.0 36 26602.0 37 29133.0 38 30604.0 39 31565.5 40 32548.0 41 33908.0 42 35298.0 43 36768.5 44 38209.5 45 39114.5 46 39208.0 47 39204.5 48 40341.5 49 40041.0 50 37702.0 51 35721.5 52 33794.5 53 32285.5 54 30589.5 55 28083.0 56 25136.0 57 22165.5 58 19652.5 59 17225.0 60 14396.5 61 11573.0 62 9077.0 63 6911.5 64 5315.5 65 4152.5 66 3096.0 67 2384.0 68 1845.5 69 1270.0 70 907.0 71 676.5 72 527.0 73 418.0 74 311.5 75 245.5 76 180.5 77 133.0 78 84.0 79 44.0 80 30.5 81 22.5 82 19.0 83 14.0 84 8.0 85 5.0 86 5.0 87 4.5 88 3.5 89 4.5 90 2.5 91 2.5 92 2.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34133155051281855 2 0.29215584736789224 3 0.29872548943833427 4 0.2915761730675591 5 0.29215584736789224 6 0.2285848990980268 7 0.21757108739169753 8 0.22375427992858415 9 0.22549330282958352 10-11 0.22974424769869303 12-13 0.23375366160933042 14-15 0.22810183718108254 16-17 0.2182956802671139 18-19 0.21080822055447782 20-21 0.20945564718703383 22-23 0.21800584311694737 24-25 0.21607359544917032 26-27 0.22273984990300116 28-29 0.22892304243988779 30-31 0.23162818917477568 32-33 0.22868151148141563 34-35 0.2283916743312491 36-37 0.21501085923189292 38-39 0.20660558187706266 40-41 0.1999393274232318 42-43 0.21148450723819978 44-45 0.21293369298903256 46-47 0.22017962174319655 48-49 0.23085529010766484 50-51 0.22530007806280578 52-53 0.23819783124521768 54-55 0.22249831894452904 56-57 0.1963163630461498 58-59 0.17931258356971164 60-61 0.19708926211326064 62-63 0.18824922903318056 64-65 0.19733079307173276 66-67 0.19800707975545473 68-69 0.1952536268288724 70-71 0.19636466923784424 72-73 0.20781323666942333 74-75 0.21303030537242143 76-77 0.21288538679733815 78-79 0.20873105431161745 80-81 0.20747509332756237 82-83 0.19602652589598324 84-85 0.19076115100129074 86-87 0.19592991351259437 88-89 0.20544623327639644 90-91 0.20114698221559246 92-93 0.19679942496309408 94-95 0.19824861071392688 96-97 0.20563945804317413 98-99 0.20501147755114657 100-101 0.18733141139098644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035064.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.66982140483086 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27286329569084 60.26218019785028 2 9.285338382481129 13.123864103467248 3 2.428002854762411 5.147595843494371 4 0.9815019052447927 2.774502574086029 5 0.5299059211362214 1.8724178404029574 6 0.3147522389372858 1.3346090707481182 7 0.21580419120263183 1.0675590555492773 8 0.1608643753599985 0.9094605341672615 9 0.12217544115459324 0.7770704945806375 >10 0.6429921964941182 8.199013065486556 >50 0.034185646377636617 1.6587000832314485 >100 0.010657142119716643 1.3455099524673002 >500 2.7325972532278993E-4 0.11480517903495499 >1k 5.465194506455799E-4 0.9230761241162067 >5k 1.3662986266139496E-4 0.4896358813172891 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5071 0.4899213961648748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4243 0.4099263427189043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2628 0.2538973435459063 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1565 0.15119838000355534 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1124 0.10859231892907106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.898371501665597E-4 0.0 0.0 0.0 0.0 12-13 2.898371501665597E-4 0.0 0.0 0.0 0.0 14-15 2.898371501665597E-4 0.0 0.0 9.661238338885325E-5 0.0 16-17 2.898371501665597E-4 0.0 0.0 2.415309584721331E-4 0.0 18-19 2.898371501665597E-4 0.0 0.0 2.898371501665597E-4 0.0 20-21 2.898371501665597E-4 0.0 0.0 2.898371501665597E-4 0.0 22-23 2.898371501665597E-4 0.0 0.0 2.898371501665597E-4 0.0 24-25 2.898371501665597E-4 0.0 0.0 5.796743003331195E-4 0.0 26-27 2.898371501665597E-4 0.0 0.0 0.0010144300255829591 0.0 28-29 2.898371501665597E-4 0.0 0.0 0.0020288600511659182 0.0 30-31 2.898371501665597E-4 0.0 0.0 0.004106026294026263 0.0 32-33 3.3814334186098636E-4 0.0 0.0 0.005361987278081355 0.0 34-35 3.86449533555413E-4 0.0 0.0 0.007197622562469567 0.0 36-37 4.8306191694426625E-4 0.0 0.0 0.010240912639218443 0.0 38-39 5.796743003331195E-4 0.0 0.0 0.013574039866133881 0.0 40-41 7.245928754163993E-4 0.0 0.0 0.021109805770464438 0.0 42-43 7.72899067110826E-4 0.0 0.0 0.029321858358516962 0.0 44-45 7.72899067110826E-4 0.0 0.0 0.0393695462309577 0.0 46-47 8.695114504996792E-4 0.0 0.0 0.056614856665868005 0.0 48-49 8.695114504996792E-4 0.0 0.0 0.06941599746489105 0.0 50-51 8.695114504996792E-4 0.0 0.0 0.08579179644930168 0.0 52-53 8.695114504996792E-4 0.0 0.0 0.10163622732507362 0.0 54-55 8.695114504996792E-4 0.0 0.0 0.12511303648856498 4.830619169442662E-5 56-57 8.695114504996792E-4 0.0 0.0 0.17704219256007359 9.661238338885325E-5 58-59 8.695114504996792E-4 0.0 0.0 0.22670095762194414 9.661238338885325E-5 60-61 8.695114504996792E-4 0.0 0.0 0.2689688753545675 9.661238338885325E-5 62-63 8.695114504996792E-4 0.0 0.0 0.31548773795630025 9.661238338885325E-5 64-65 8.695114504996792E-4 0.0 0.0 0.3647600534846155 9.661238338885325E-5 66-67 8.695114504996792E-4 0.0 0.0 0.44026263110300423 9.661238338885325E-5 68-69 8.695114504996792E-4 0.0 0.0 0.534218173948664 9.661238338885325E-5 70-71 8.695114504996792E-4 0.0 0.0 0.5940212392663642 9.661238338885325E-5 72-73 8.695114504996792E-4 0.0 0.0 0.6598625785458677 9.661238338885325E-5 74-75 8.695114504996792E-4 0.0 0.0 0.7651700764397178 1.4491857508327986E-4 76-77 8.695114504996792E-4 0.0 0.0 0.8958866311648361 1.932247667777065E-4 78-79 8.695114504996792E-4 0.0 0.0 1.0311922741009252 1.932247667777065E-4 80-81 9.661238338885325E-4 0.0 0.0 1.1656767117782088 1.932247667777065E-4 82-83 9.661238338885325E-4 0.0 0.0 1.3324296855073696 1.932247667777065E-4 84-85 9.661238338885325E-4 0.0 0.0 1.516476275863135 1.932247667777065E-4 86-87 9.661238338885325E-4 0.0 0.0 1.7099908797910082 1.932247667777065E-4 88-89 9.661238338885325E-4 0.0 0.0 1.974708810276466 1.932247667777065E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2830 0.0 46.02976 1 GTATCAA 4725 0.0 38.335197 1 ATCAACG 5850 0.0 30.788565 3 TCAACGC 5970 0.0 30.255167 4 TATCAAC 6025 0.0 30.209797 2 CAACGCA 6055 0.0 29.906046 5 AACGCAG 6195 0.0 29.217457 6 TCTCGCG 35 0.008379026 27.15219 50-51 ACGCAGA 7010 0.0 25.820562 7 CGCAGAG 7060 0.0 25.570406 8 GCAGAGT 7400 0.0 23.751259 9 GTACATG 3890 0.0 23.709732 1 GTGTAGC 615 0.0 23.191065 1 TACATGG 3990 0.0 22.630117 2 ACATGGG 4050 0.0 21.942831 3 GTACCTG 760 0.0 21.894196 1 GAGTACT 4500 0.0 21.849041 12-13 CGTTAGT 55 0.0028812957 21.586824 90-91 GTGGTAT 1610 0.0 21.556166 1 TGGTATC 1515 0.0 21.330564 2 >>END_MODULE