FastQCFastQC Report
Thu 26 May 2016
SRR937872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035064
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.5709791858281227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.5301121476546378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.3259701815539908No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.1378658710958936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.046.0379031
GTATCAA55300.036.5248571
ATCAACG67550.029.6800653
TATCAAC69150.029.062032
TCAACGC68850.028.981654
CAACGCA69750.028.6076955
AACGCAG72100.027.932096
GTACCTG8350.025.043261
ACGCAGA80900.024.838637
CGCAGAG81650.024.435938
GAGTACT51450.023.1290712-13
GCAGAGT85000.023.0257669
GTACATG41950.022.997931
TACATGG42150.022.4302042
GTACTTT53850.022.05094114-15
ACATGGG42450.021.1525083
AGAGTAC76950.020.86622410-11
AGTACTT53200.020.8502412-13
TACCTGG10150.020.5951232
CAGAGTA84000.020.2742910-11
Produced by FastQC (version 0.11.5)