FastQCFastQC Report
Thu 26 May 2016
SRR937871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925694
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193981.0073251513480335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145430.7552082521937546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101390.526511481055661No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43190.22428277805300323No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35780.18580314421709784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.17344396357884484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30490.15833252842871193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.14207864800949685No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26440.13730114961151668No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24760.1285770221021616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA222750.030.3849071
GGTATCA151650.029.7955841
TATCAAC266900.025.24522
ATCAACG269400.024.9756243
GTACATG180850.024.8006971
TCAACGC271250.024.7364544
TACATGG178550.024.607622
CAACGCA274600.024.4000455
AACGCAG280750.023.8914556
ACATGGG179750.023.6232723
GAGTACT140950.023.57369612-13
AGTACTT145250.022.09094212-13
CATGGGA136750.022.0466234
GTACTTT151900.021.87462614-15
ATGGGAT51700.021.615115
ACGCAGA308800.021.5366617
CGCAGAG307850.021.5094178
GGACCGA2701.0022632E-921.1127666
GCAGAGT313850.020.5987599
TAGCCCT14250.020.3561274
Produced by FastQC (version 0.11.5)