FastQCFastQC Report
Thu 26 May 2016
SRR937871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925694
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233951.2148866850081061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192320.9987048824995041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138340.7183903569310596No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45140.23440899748350466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45020.23378584551855072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.2046015618265415No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39290.20403033919200042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31040.16118864160141746No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.11258278833501065No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21640.11237507101335933No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.10515689408597628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160150.031.0419351
GTATCAA232700.029.5744171
TATCAAC287250.023.8544982
ATCAACG290650.023.575453
TCAACGC293750.023.3266544
CAACGCA297100.023.0476365
GAGTACT175400.022.65510412-13
AACGCAG303400.022.5904086
AGTACTT179350.022.4872312-13
TACATGG166350.021.9383262
GTACATG171050.021.7283971
GTACTTT185750.021.35162514-15
ACATGGG168250.020.8997783
ACGCAGA335100.020.356817
CGCAGAG334700.020.3527538
TACTTTT199500.020.14191614-15
GCGTATC1200.00474877919.8030831
GCAGAGT342450.019.6563289
TCCGTTG1707.9523335E-519.560458
CATGGGA128800.019.405954
Produced by FastQC (version 0.11.5)