##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937871_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1925694 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00340708336839 31.0 31.0 34.0 30.0 34.0 2 32.27364939600996 33.0 31.0 34.0 31.0 34.0 3 32.342544038668656 34.0 31.0 34.0 30.0 34.0 4 36.02052039420593 37.0 35.0 37.0 35.0 37.0 5 35.93788992435974 37.0 35.0 37.0 35.0 37.0 6 35.98944847935342 37.0 35.0 37.0 35.0 37.0 7 35.92807528091171 37.0 35.0 37.0 35.0 37.0 8 35.91540244711776 37.0 35.0 37.0 35.0 37.0 9 37.56199635040666 39.0 37.0 39.0 35.0 39.0 10-11 37.48385205541483 39.0 37.0 39.0 35.0 39.0 12-13 37.3671099873604 39.0 37.0 39.0 34.0 39.0 14-15 38.454495366345846 40.0 38.0 41.0 34.0 41.0 16-17 38.43795327814284 40.0 38.0 41.0 34.0 41.0 18-19 38.522453982823855 40.0 38.0 41.0 34.0 41.0 20-21 38.481842390327856 40.0 38.0 41.0 34.0 41.0 22-23 38.4189100137405 40.0 38.0 41.0 34.0 41.0 24-25 38.42680015620343 40.0 38.0 41.0 34.0 41.0 26-27 38.253058897207964 40.0 38.0 41.0 34.0 41.0 28-29 38.07980110027866 40.0 37.0 41.0 33.0 41.0 30-31 37.99647633528484 40.0 37.0 41.0 33.0 41.0 32-33 37.80104523356255 40.0 36.5 41.0 33.0 41.0 34-35 37.61052405003079 40.0 36.0 41.0 33.0 41.0 36-37 37.409586362111526 40.0 36.0 41.0 33.0 41.0 38-39 37.23545589278463 40.0 35.5 41.0 33.0 41.0 40-41 36.985699700990914 39.5 35.0 41.0 32.0 41.0 42-43 36.68184223453986 39.0 35.0 41.0 31.0 41.0 44-45 36.2320181711113 39.0 35.0 41.0 30.0 41.0 46-47 35.636142606250004 39.0 35.0 41.0 28.0 41.0 48-49 35.11497465329383 39.0 35.0 41.0 25.0 41.0 50-51 34.779999574179485 39.0 35.0 40.0 23.5 41.0 52-53 34.50768034796806 39.0 34.5 40.0 23.0 41.0 54-55 34.28539477196273 38.0 34.0 40.0 22.0 41.0 56-57 34.02785385424683 38.0 34.0 40.0 20.5 41.0 58-59 33.78178931855217 38.0 34.0 40.0 19.0 41.0 60-61 33.46679041426104 38.0 33.0 40.0 18.0 41.0 62-63 33.092312433855014 37.0 33.0 40.0 14.0 41.0 64-65 32.68693312644688 36.5 32.0 39.5 10.0 41.0 66-67 32.273300430909586 36.0 32.0 39.0 8.0 40.5 68-69 31.839707139348207 35.5 31.0 39.0 7.0 40.0 70-71 31.446605743176228 35.0 31.0 38.0 3.5 40.0 72-73 30.956439600476504 35.0 30.5 37.5 2.0 39.5 74-75 30.47331871003389 35.0 30.0 37.0 2.0 39.0 76-77 29.236540436850298 33.5 28.5 35.5 2.0 38.0 78-79 29.802615836160886 34.0 30.0 36.0 2.0 38.5 80-81 29.621420900724623 34.0 30.0 36.0 2.0 37.5 82-83 29.237740783322792 34.0 29.0 35.0 2.0 37.0 84-85 28.853430763143052 34.0 29.0 35.0 2.0 36.5 86-87 28.47267348810351 34.0 29.0 35.0 2.0 36.0 88-89 28.0094719098673 34.0 28.0 35.0 2.0 36.0 90-91 27.44520235302182 33.0 26.5 35.0 2.0 35.0 92-93 27.009768426343957 33.0 26.0 35.0 2.0 35.0 94-95 26.538328779131056 33.0 25.0 35.0 2.0 35.0 96-97 25.95923547562593 32.5 24.5 34.5 2.0 35.0 98-99 25.083214934459992 32.0 19.0 34.0 2.0 35.0 100-101 22.46908387313872 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3245797041173972 1101 2 0.3083720554181255 1101 3 0.325158273981522 1101 4 0.1659740286751159 1101 5 0.09761550692147836 1101 6 0.14320524824553615 1101 7 0.1633361401724045 1101 8 0.20153554962815434 1101 9 0.2744879810204992 1101 10-11 0.29100708134905773 1101 12-13 0.2641235995683857 1101 14-15 0.3498846378038607 1101 16-17 0.2668264434108565 1101 18-19 0.2986592589448307 1101 20-21 0.32403382502590006 1101 22-23 0.19231218630887525 1101 24-25 0.19233535708753635 1101 26-27 0.0050664543668759165 1101 28-29 0.08305395729856002 1101 30-31 0.2348615310181401 1101 32-33 0.34547306929225385 1101 34-35 0.4261001854824755 1101 36-37 0.33267686639123895 1101 38-39 0.3721472156427552 1101 40-41 0.3293650505102548 1101 42-43 0.46347807247421713 1101 44-45 0.2540583083249075 1101 46-47 0.2705423787167689 1101 48-49 0.3744811868713285 1101 50-51 0.48413667955720285 1101 52-53 0.3606336031686226 1101 54-55 0.291528942252377 1101 56-57 0.18760981733117887 1101 58-59 0.21718186216959623 1101 60-61 0.39483899144492085 1101 62-63 0.2993093288448847 1101 64-65 0.24796639284285504 1101 66-67 0.18650041383268245 1101 68-69 0.4082138781679099 1101 70-71 0.415390736466577 1101 72-73 0.28802159256147064 1101 74-75 0.21118969631096363 1101 76-77 0.19897605343438585 1101 78-79 0.29135403303861906 1101 80-81 0.25241965252234166 1101 82-83 0.16067633189792474 1101 84-85 0.09297085028225638 1101 86-87 -0.07528786225322293 1101 88-89 0.018267630271406432 1101 90-91 -0.011290596073273207 1101 92-93 0.027770594326135267 1101 94-95 0.03470479165344287 1101 96-97 0.30593176027441515 1101 98-99 0.30330580587970246 1101 100-101 0.5022011866279676 1102 1 0.25553465482941107 1102 2 0.18964392757678894 1102 3 0.10822963200136826 1102 4 0.08931239873732011 1102 5 0.08406474388976193 1102 6 0.1341587598921521 1102 7 0.09011387652254399 1102 8 0.1093424793821427 1102 9 0.1302164424633574 1102 10-11 0.1653092981638835 1102 12-13 0.24533136790503107 1102 14-15 0.2748942860831889 1102 16-17 0.29569213282129425 1102 18-19 0.26893601738462536 1102 20-21 0.2985545181321285 1102 22-23 0.2996860882038632 1102 24-25 0.15989424429751153 1102 26-27 0.21826791698061498 1102 28-29 0.25747914879237754 1102 30-31 0.33942402243280867 1102 32-33 0.38861036267190485 1102 34-35 0.3080268323947095 1102 36-37 0.2248077951771279 1102 38-39 0.29192324473803666 1102 40-41 0.3103648084856232 1102 42-43 0.3636939322830912 1102 44-45 0.29718840637759314 1102 46-47 0.034818888590720576 1102 48-49 0.059805990471609505 1102 50-51 0.1518852617507278 1102 52-53 0.29047916528718787 1102 54-55 0.18892292565079316 1102 56-57 0.2291955365695273 1102 58-59 0.2293844825768545 1102 60-61 0.11250744386092038 1102 62-63 -0.04051746001151457 1102 64-65 0.08409609188195333 1102 66-67 0.15292898058896043 1102 68-69 0.03153981993809296 1102 70-71 0.11128612949496386 1102 72-73 0.0679224278735191 1102 74-75 0.1510295675800215 1102 76-77 -0.010669609418755499 1102 78-79 0.12486986602853634 1102 80-81 0.2189561491830574 1102 82-83 0.03336221662878813 1102 84-85 0.009358534498979054 1102 86-87 -0.07104719818995875 1102 88-89 0.023051868580633794 1102 90-91 0.25165085358887396 1102 92-93 0.27758057228227173 1102 94-95 0.16178276937408853 1102 96-97 -0.10707804499387308 1102 98-99 -0.0509168698661604 1102 100-101 0.16693100210054013 1103 1 0.24320385370316444 1103 2 0.1840114611085113 1103 3 0.13165285560572926 1103 4 0.056123850967331634 1103 5 0.04397700999946608 1103 6 0.056688009656490124 1103 7 0.07505987458796426 1103 8 0.01560029626229209 1103 9 0.013489705190622203 1103 10-11 0.06922651778817368 1103 12-13 0.13915487738385934 1103 14-15 0.1655571575197783 1103 16-17 0.1304228008680539 1103 18-19 0.06842341487952552 1103 20-21 0.10640620842657 1103 22-23 0.08768182473110642 1103 24-25 0.07297423370044243 1103 26-27 0.1421827953915411 1103 28-29 0.07069221769182121 1103 30-31 0.1357292180781542 1103 32-33 0.19103659904819636 1103 34-35 0.27884360780456774 1103 36-37 0.25627185447143574 1103 38-39 0.2271880790971892 1103 40-41 0.1634992619047182 1103 42-43 0.2862601485742431 1103 44-45 0.3085395945433689 1103 46-47 0.3999951881181616 1103 48-49 0.3221709856351538 1103 50-51 0.373606792904269 1103 52-53 0.5215920425768061 1103 54-55 0.39263066828983284 1103 56-57 0.4470750133699468 1103 58-59 0.4306776949064144 1103 60-61 0.6221082669822451 1103 62-63 0.6147776868996218 1103 64-65 0.5055785728287887 1103 66-67 0.6623807053854947 1103 68-69 0.5108835408654535 1103 70-71 0.5030937310450909 1103 72-73 0.5015958532535016 1103 74-75 0.5425272664137246 1103 76-77 0.6225971841663203 1103 78-79 0.5827107744193221 1103 80-81 0.6463495777668058 1103 82-83 0.7773671701433784 1103 84-85 0.7952521078682935 1103 86-87 0.6343961228828086 1103 88-89 0.7848081482693736 1103 90-91 0.6974647024820335 1103 92-93 0.7327389430442146 1103 94-95 0.6101282092449551 1103 96-97 0.4435174241421542 1103 98-99 0.49033469350987247 1103 100-101 0.4557917553551363 1104 1 0.3937607544295574 1104 2 0.24619063786396111 1104 3 0.22430015181235063 1104 4 0.14349269681075327 1104 5 0.06958637238848553 1104 6 0.15343538657336353 1104 7 0.14496302228771896 1104 8 0.14404258843339335 1104 9 0.1545712225433249 1104 10-11 0.20072369292942227 1104 12-13 0.14159636245246787 1104 14-15 0.20113020029620543 1104 16-17 0.26121376132002894 1104 18-19 0.2681126804162446 1104 20-21 0.1897395417598986 1104 22-23 0.23751233320567877 1104 24-25 0.22190078737276053 1104 26-27 0.23377682283302903 1104 28-29 0.12985283108972112 1104 30-31 0.15788821727105073 1104 32-33 0.20252166765190793 1104 34-35 0.30658372079891905 1104 36-37 0.28715966722365494 1104 38-39 0.18918969330219682 1104 40-41 0.14116270016137378 1104 42-43 0.35921010821911636 1104 44-45 0.17111425152481274 1104 46-47 0.22951899764197492 1104 48-49 0.3038336167893121 1104 50-51 0.35305392769038946 1104 52-53 0.47319817655582597 1104 54-55 0.35048781114697647 1104 56-57 0.4043591134506599 1104 58-59 0.46394646003958684 1104 60-61 0.4164787270306718 1104 62-63 0.36204160941777985 1104 64-65 0.24318551391030496 1104 66-67 0.30405947858968574 1104 68-69 0.4099876570882124 1104 70-71 0.3690178634099013 1104 72-73 0.2311358048273533 1104 74-75 0.17926657230557552 1104 76-77 0.1393849162082894 1104 78-79 0.17571319572683208 1104 80-81 0.13748759229465435 1104 82-83 0.10230260194321517 1104 84-85 0.245441776956266 1104 86-87 0.12016690577223699 1104 88-89 0.20689047272457728 1104 90-91 0.41223064275644816 1104 92-93 0.2861247380401757 1104 94-95 0.17321134080266276 1104 96-97 0.1622422021889669 1104 98-99 0.4047374376583761 1104 100-101 0.20571508061744126 1105 1 0.19410964646161943 1105 2 0.17661968076824763 1105 3 0.1748847817039092 1105 4 0.013200381864969302 1105 5 0.03985039879113117 1105 6 0.045406155078779875 1105 7 0.026344446144392464 1105 8 0.07847917494918732 1105 9 0.13816386752210974 1105 10-11 0.11143797864370697 1105 12-13 0.22364492445152706 1105 14-15 0.16882232665169994 1105 16-17 0.12172766985373329 1105 18-19 0.16505516037852885 1105 20-21 -0.008303834006248678 1105 22-23 0.10334920260690694 1105 24-25 0.050528793030473196 1105 26-27 0.047253956260654206 1105 28-29 0.07190846522455985 1105 30-31 -0.05599438527491429 1105 32-33 0.023422186275197987 1105 34-35 0.12929470618316685 1105 36-37 0.23401020942073814 1105 38-39 0.2623250068335494 1105 40-41 0.33132064452723853 1105 42-43 0.4520837527264234 1105 44-45 0.43279741984164133 1105 46-47 0.4481800066000119 1105 48-49 0.42249462574734764 1105 50-51 0.5916842907266968 1105 52-53 0.6410152439768524 1105 54-55 0.6187975706943618 1105 56-57 0.47577589798625297 1105 58-59 0.5713580583422768 1105 60-61 0.4609978217972923 1105 62-63 0.2948846080033576 1105 64-65 0.4134801862346791 1105 66-67 0.5217115295090693 1105 68-69 0.5698603586440889 1105 70-71 0.4548641623255065 1105 72-73 0.5012913918146218 1105 74-75 0.3621142856483388 1105 76-77 0.3781402203101294 1105 78-79 0.46012149369948574 1105 80-81 0.5567803787161516 1105 82-83 0.47250297912379224 1105 84-85 0.6794752517323168 1105 86-87 0.40016219101504547 1105 88-89 0.5854666160531998 1105 90-91 0.7497577526102894 1105 92-93 0.7660610888181125 1105 94-95 0.65918965291959 1105 96-97 0.4716245689970755 1105 98-99 0.2828350331322085 1105 100-101 0.33063492974521225 1106 1 0.05292284234164413 1106 2 0.03708349500681862 1106 3 0.0321115071237017 1106 4 0.03925734882540155 1106 5 0.01865688520900477 1106 6 -0.04176607862810755 1106 7 -0.03171280188810499 1106 8 -0.11797206358125578 1106 9 0.014232394704499995 1106 10-11 -0.0809155012097662 1106 12-13 -0.3314920075109029 1106 14-15 -0.09532126490525883 1106 16-17 0.022591966448231915 1106 18-19 -0.0827069166533505 1106 20-21 -0.00214461574925906 1106 22-23 0.019201893645245605 1106 24-25 0.02566910239107756 1106 26-27 -0.11303407460287218 1106 28-29 -0.009959439972547557 1106 30-31 -0.1553352259523919 1106 32-33 -0.11869170231145887 1106 34-35 -0.4351836784684764 1106 36-37 -0.24430553424154056 1106 38-39 -0.12956946420696625 1106 40-41 -0.22755295917928464 1106 42-43 -0.39271754745854537 1106 44-45 -0.20864994261437886 1106 46-47 -0.08640591078293625 1106 48-49 -0.01591455170886036 1106 50-51 0.16255026468672185 1106 52-53 0.07589277362541225 1106 54-55 0.0818820419162094 1106 56-57 0.09504134788289065 1106 58-59 0.0927720094901403 1106 60-61 -0.008899020222074228 1106 62-63 -0.06198998398881628 1106 64-65 -0.2890695227197284 1106 66-67 -0.35251790236269187 1106 68-69 -0.08751234291178278 1106 70-71 -0.10014651387947637 1106 72-73 -0.17133672264517585 1106 74-75 -0.27141795150394543 1106 76-77 -0.22827762957925657 1106 78-79 -0.2640326833940492 1106 80-81 -0.2639753197932606 1106 82-83 -0.336397031756416 1106 84-85 -0.45943917542468427 1106 86-87 -0.13700800265267077 1106 88-89 -0.14966172507761755 1106 90-91 -0.33498547445966764 1106 92-93 -0.44282214840670875 1106 94-95 -0.26185505113872765 1106 96-97 -0.10617583810407183 1106 98-99 -0.19450477845883896 1106 100-101 -0.29578675088438544 1107 1 0.15449808161849887 1107 2 0.1686791171266293 1107 3 0.05685007500743211 1107 4 0.03281681355421995 1107 5 -0.06324004850245046 1107 6 -0.0274094674973 1107 7 0.044363943946706286 1107 8 0.050709255100059636 1107 9 0.08160655280446605 1107 10-11 0.15053168064063271 1107 12-13 0.12020619348164985 1107 14-15 0.10754341074167684 1107 16-17 0.17788042798668613 1107 18-19 0.19194524570042404 1107 20-21 0.11354906556942268 1107 22-23 0.09548468343609784 1107 24-25 0.06087160150486426 1107 26-27 0.01455255247972076 1107 28-29 0.11272533492996928 1107 30-31 0.11273460744002506 1107 32-33 0.07484117610044905 1107 34-35 0.04693702341028683 1107 36-37 0.1097710343511551 1107 38-39 0.166409355360571 1107 40-41 0.2479599351844115 1107 42-43 0.25749735860313194 1107 44-45 0.31394067241574675 1107 46-47 0.16451131715042067 1107 48-49 0.040131619861512036 1107 50-51 0.2155500874446119 1107 52-53 0.18040247763109107 1107 54-55 0.1928610830363766 1107 56-57 0.04978922097753724 1107 58-59 0.13348563054650953 1107 60-61 0.2016169758785935 1107 62-63 0.13655773845003694 1107 64-65 0.2751286782728215 1107 66-67 0.25942046169260635 1107 68-69 0.3195729105444407 1107 70-71 0.3080808434406208 1107 72-73 0.4087855744126081 1107 74-75 0.35287640333476133 1107 76-77 0.2952988947029098 1107 78-79 0.34903116807705814 1107 80-81 0.47154289183382403 1107 82-83 0.32395390762678034 1107 84-85 0.4573234973863727 1107 86-87 0.42073526214704415 1107 88-89 0.35721305336974396 1107 90-91 0.41936581940775497 1107 92-93 0.22044885478364762 1107 94-95 0.1879732455645673 1107 96-97 0.21884742492322218 1107 98-99 0.187679065922584 1107 100-101 0.3935875844576877 1108 1 0.0753784974171623 1108 2 0.059315230166632205 1108 3 7.319848195237455E-4 1108 4 0.003603590483287178 1108 5 0.03702929476291672 1108 6 -0.07004145621854718 1108 7 -0.07732660589231699 1108 8 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-0.6321823688247292 2316 54-55 -0.607532131872965 2316 56-57 -0.7235305894390436 2316 58-59 -0.8628992868061545 2316 60-61 -0.7470845615326169 2316 62-63 -0.8235017361255643 2316 64-65 -0.602433126708334 2316 66-67 -0.42407238954217874 2316 68-69 -0.5510094939089321 2316 70-71 -0.506619092227055 2316 72-73 -0.5547412810497327 2316 74-75 -0.5484745754355735 2316 76-77 -0.514379837556465 2316 78-79 -0.5412139512003158 2316 80-81 -0.5583040234969623 2316 82-83 -0.5124654078247914 2316 84-85 -0.5418821953677053 2316 86-87 -0.6450386294332979 2316 88-89 -0.6468661410320315 2316 90-91 -0.3891877536619468 2316 92-93 -0.3311056241901831 2316 94-95 -0.5188440112526891 2316 96-97 -0.5583927184459512 2316 98-99 -0.5492857613648212 2316 100-101 -0.2277668078644446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 33.0 5 52.0 6 113.0 7 282.0 8 731.0 9 1322.0 10 2400.0 11 4050.0 12 6442.0 13 9217.0 14 12025.0 15 17591.0 16 24711.0 17 35580.0 18 31177.0 19 17001.0 20 12355.0 21 11592.0 22 12010.0 23 12785.0 24 14401.0 25 17027.0 26 20304.0 27 24748.0 28 30589.0 29 37979.0 30 46213.0 31 57828.0 32 72702.0 33 93848.0 34 125805.0 35 179513.0 36 259527.0 37 388790.0 38 316263.0 39 28677.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.336275096396754 20.076026708549392 15.712671187342108 19.87502700771174 2 15.306897149806769 21.913346564926723 40.75533288258675 22.02442340267976 3 19.537475839879026 27.361512265188548 30.687430090138935 22.41358180479349 4 12.060015765744714 17.75806540395307 38.12480072119454 32.05711810910768 5 12.062585490610255 39.788115759676124 33.43366655947601 14.715632190237615 6 28.979630200852263 37.88924927844196 17.693049882276206 15.438070638429574 7 26.358964612238495 35.96983736772301 20.207831566178218 17.46336645386027 8 26.548091233602015 34.64776854474283 19.518677422269583 19.285462799385574 9 27.58532767926784 16.547073418725926 20.859544662859207 35.00805423914703 10-11 24.40382532219553 28.44805561008135 26.191544450987543 20.956574616735576 12-13 26.36074448116298 26.219516469980025 27.678301337836146 19.741437711020847 14-15 23.237051161814907 26.301712525458353 28.411107891492627 22.05012842123411 16-17 19.78325736072294 29.93583092640887 28.90666949162224 21.37424222124595 18-19 19.61417546089877 27.337962313846333 33.653269938006765 19.39459228724813 20-21 21.406542264762727 25.763750627046665 33.935531813465694 18.894175294724917 22-23 20.560107037208308 26.217884488406856 33.59273879353612 19.629269680848715 24-25 19.96245501612409 26.324226640840426 34.31764718101044 19.395671162025042 26-27 20.05386173083 26.357922418231773 34.19614923936923 19.392066611569003 28-29 19.68972430179659 26.554202176951502 34.396701000091134 19.35937252116077 30-31 19.888154608156853 26.61894880495032 34.27849907617721 19.214397510715617 32-33 19.66589707398995 26.644575929509053 33.93981598322475 19.749711013276254 34-35 20.030025538844697 26.539003600779772 34.04624514590584 19.384725714469692 36-37 20.202508809810904 26.364261403940603 33.96458627383167 19.468643512416822 38-39 20.208350859482348 26.53240857581734 33.86677737999911 19.392463184701203 40-41 20.209764263167585 26.657753823248914 33.73886857433366 19.39361333924984 42-43 19.858852356126445 26.89091647033253 33.43588670276289 19.814344470778135 44-45 20.152422970627732 27.253369434603837 32.81133970402359 19.782867890744843 46-47 20.371149310326565 27.512574687359464 31.780464601333335 20.33581140098063 48-49 20.585435692275098 27.673919116952124 31.4839740893413 20.256671101431483 50-51 21.010035862395583 28.107217449916753 30.87720582813261 20.005540859555047 52-53 20.750596927653095 28.380859056527154 30.586219825164328 20.28232419065542 54-55 20.518717157927043 28.39463507423042 30.59329810099533 20.49334966684721 56-57 20.94930451047778 28.773808299761022 29.945256099878797 20.3316310898824 58-59 20.835501382878068 28.337627888958473 30.36497491294048 20.46189581522298 60-61 20.870449822245902 28.17830870325192 30.60803014393772 20.34321133056446 62-63 21.060563100887265 28.03194588548336 30.687637807460582 20.21985320616879 64-65 20.93650393053102 28.033893235373846 30.67153971503261 20.358063119062532 66-67 20.778015614111066 28.27404042386797 30.427030462783804 20.52091349923716 68-69 20.789543925462716 28.553004786845676 30.248860930137393 20.408590357554214 70-71 20.85411804783107 28.44387529898312 29.93315656589261 20.768850087293206 72-73 20.87800553982097 28.344794136555446 29.8339455801389 20.943254743484687 74-75 20.85539031642618 28.636870655462392 29.62334618064968 20.884392847461747 76-77 20.819169608463234 28.38623374222488 30.063473220563598 20.731123428748287 78-79 20.708663993344736 28.52823449623876 29.90999608452849 20.853105425888018 80-81 20.477941978320544 28.99910369975707 29.690620628199493 20.832333693722887 82-83 20.643361676538493 28.92354500898902 29.469896898864924 20.96319641560756 84-85 20.68530116552816 28.596035661957625 29.428540938628085 21.29012223388613 86-87 20.611374392816302 28.648866330787758 29.537403138816448 21.20235613757949 88-89 20.907787010812726 28.221851446803075 29.673406055167646 21.196955487216556 90-91 20.601141893823662 28.44207344069846 29.718556696479926 21.23822796899795 92-93 20.409644860900215 28.570449319405007 29.69006079968094 21.329845020013835 94-95 20.333111075799167 28.678336225797036 29.476853539555094 21.511699158848707 96-97 20.309950594435044 28.536854765087288 29.317378565857293 21.83581607462037 98-99 20.37609708398051 28.427048621442996 29.22022586389278 21.976628430683714 100-101 20.619603490538086 28.456226613340302 29.534405348772047 21.38976454734957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2937.0 1 1982.5 2 1316.0 3 1623.0 4 1287.0 5 1167.5 6 2298.0 7 4870.5 8 7301.5 9 8100.0 10 6992.5 11 5591.5 12 5512.0 13 5912.5 14 6228.0 15 6373.0 16 6475.5 17 6694.0 18 6973.0 19 7387.0 20 8031.5 21 8793.5 22 9793.5 23 11292.0 24 12805.5 25 14816.5 26 18046.5 27 21364.5 28 23821.0 29 26429.5 30 29810.5 31 33169.5 32 36605.0 33 40700.5 34 45527.0 35 48825.5 36 53195.5 37 57720.0 38 58511.0 39 59153.0 40 60490.0 41 63085.0 42 64996.0 43 65321.0 44 66904.0 45 68110.0 46 68921.0 47 71531.5 48 81949.0 49 79771.5 50 66927.0 51 60733.0 52 53923.0 53 49398.0 54 46036.0 55 41369.0 56 35420.0 57 30278.5 58 26885.5 59 24342.0 60 20799.5 61 17031.5 62 13443.5 63 9345.0 64 6177.0 65 4489.0 66 3316.5 67 2550.5 68 1932.5 69 1444.0 70 1143.0 71 829.5 72 632.5 73 577.5 74 476.5 75 366.5 76 271.0 77 156.0 78 99.5 79 79.0 80 53.5 81 42.0 82 31.0 83 18.5 84 12.5 85 8.0 86 6.0 87 4.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016513527071279236 2 0.0 3 0.0 4 0.0 5 0.04024523106994154 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004388028419883948 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.413993085090362E-4 24-25 2.077173216513112E-4 26-27 0.0011684099342886252 28-29 0.0010126219430501419 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.413993085090362E-4 42-43 0.00999639610446935 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.19293304128278E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.231519649539334E-4 84-85 2.59646652064139E-5 86-87 0.0 88-89 0.0 90-91 0.010827265391074594 92-93 0.0013501625907335224 94-95 0.0 96-97 0.0 98-99 0.0010905159386693838 100-101 0.016487562406072822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1925694.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.9834596843561 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14863312210467 33.1799064964284 2 15.549787283565268 14.922651824425342 3 6.308348270516027 9.080891247395499 4 3.153194321370887 6.052046903857622 5 1.7499201294147708 4.198361099030843 6 1.0790190861227444 3.1065041290572872 7 0.6795697421507674 2.2825675127639733 8 0.4635651520510795 1.7794767827612306 9 0.3558622467084528 1.5367951591307483 >10 1.3821310590281668 11.234501098246167 >50 0.08133565144117552 2.7133891814898545 >100 0.04300254442803145 3.6929546043985364 >500 0.003032287514340222 1.0092478995365293 >1k 0.0022742156357551664 2.2767576701829624 >5k 0.0 0.0 >10k+ 3.248879479650238E-4 2.933948391295025 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23395 1.2148866850081061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19232 0.9987048824995041 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13834 0.7183903569310596 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4514 0.23440899748350466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4502 0.23378584551855072 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3940 0.2046015618265415 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3929 0.20403033919200042 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3104 0.16118864160141746 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2168 0.11258278833501065 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2164 0.11237507101335933 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 0.10515689408597628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.192933041282779E-5 0.0 8 0.0 0.0 0.0 5.192933041282779E-5 0.0 9 0.0 0.0 0.0 5.192933041282779E-5 0.0 10-11 0.0 0.0 0.0 7.78939956192417E-5 0.0 12-13 0.0 0.0 0.0 1.5578799123848336E-4 1.2982332603206947E-4 14-15 0.0 0.0 0.0 2.0771732165131117E-4 1.5578799123848336E-4 16-17 0.0 0.0 0.0 2.3368198685772506E-4 1.5578799123848336E-4 18-19 0.0 0.0 0.0 2.5964665206413894E-4 1.5578799123848336E-4 20-21 0.0 0.0 0.0 3.115759824769668E-4 1.5578799123848336E-4 22-23 0.0 0.0 0.0 5.192933041282779E-4 1.5578799123848336E-4 24-25 0.0 0.0 0.0 6.491166301603474E-4 1.5578799123848336E-4 26-27 0.0 0.0 0.0 8.049046213988308E-4 1.5578799123848336E-4 28-29 0.0 0.0 0.0 0.0017655972340361449 1.5578799123848336E-4 30-31 0.0 0.0 0.0 0.0036869824593107735 1.5578799123848336E-4 32-33 0.0 0.0 0.0 0.006205554984332921 1.5578799123848336E-4 34-35 0.0 0.0 0.0 0.00825676353563962 1.5578799123848336E-4 36-37 0.0 0.0 0.0 0.01165813467767984 1.5578799123848336E-4 38-39 0.0 0.0 0.0 0.017967548322838416 1.5578799123848336E-4 40-41 0.0 0.0 0.0 0.024147138641964924 1.5578799123848336E-4 42-43 0.0 0.0 0.0 0.029963223648201635 1.5578799123848336E-4 44-45 0.0 0.0 0.0 0.03357231211189317 1.5578799123848336E-4 46-47 0.0 0.0 0.0 0.039622079104987606 1.5578799123848336E-4 48-49 0.0 0.0 0.0 0.04761919598856308 1.5578799123848336E-4 50-51 0.0 0.0 0.0 0.05725208678014264 1.5578799123848336E-4 52-53 0.0 0.0 0.0 0.07732277298470058 1.5578799123848336E-4 54-55 0.0 0.0 0.0 0.09290157210854892 1.5578799123848336E-4 56-57 0.0 0.0 0.0 0.12678546020291906 1.5578799123848336E-4 58-59 0.0 0.0 0.0 0.18227194974902555 1.5578799123848336E-4 60-61 0.0 0.0 0.0 0.23827773259926033 1.5578799123848336E-4 62-63 0.0 0.0 0.0 0.271252857411406 1.5578799123848336E-4 64-65 0.0 0.0 0.0 0.3241948097672839 1.5578799123848336E-4 66-67 0.0 0.0 0.0 0.40668455112806084 1.5578799123848336E-4 68-69 0.0 0.0 0.0 0.5255248237778172 1.5578799123848336E-4 70-71 0.0 0.0 0.0 0.6460268350007841 1.5578799123848336E-4 72-73 0.0 0.0 0.0 0.7743442104508815 1.8175265644489728E-4 74-75 0.0 0.0 0.0 0.9235112120617295 2.0771732165131117E-4 76-77 0.0 0.0 0.0 1.0452075978841915 2.0771732165131117E-4 78-79 0.0 0.0 0.0 1.1975422886502218 2.0771732165131117E-4 80-81 0.0 0.0 0.0 1.3992617726388512 2.0771732165131117E-4 82-83 0.0 0.0 0.0 1.5847014115430593 2.5964665206413894E-4 84-85 0.0 0.0 0.0 1.8047779138326234 2.5964665206413894E-4 86-87 0.0 0.0 0.0 2.0794840717164824 2.5964665206413894E-4 88-89 0.0 0.0 0.0 2.4097546131420673 2.5964665206413894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16015 0.0 31.041935 1 GTATCAA 23270 0.0 29.574417 1 TATCAAC 28725 0.0 23.854498 2 ATCAACG 29065 0.0 23.57545 3 TCAACGC 29375 0.0 23.326654 4 CAACGCA 29710 0.0 23.047636 5 GAGTACT 17540 0.0 22.655104 12-13 AACGCAG 30340 0.0 22.590408 6 AGTACTT 17935 0.0 22.48723 12-13 TACATGG 16635 0.0 21.938326 2 GTACATG 17105 0.0 21.728397 1 GTACTTT 18575 0.0 21.351625 14-15 ACATGGG 16825 0.0 20.899778 3 ACGCAGA 33510 0.0 20.35681 7 CGCAGAG 33470 0.0 20.352753 8 TACTTTT 19950 0.0 20.141916 14-15 GCGTATC 120 0.004748779 19.803083 1 GCAGAGT 34245 0.0 19.656328 9 TCCGTTG 170 7.9523335E-5 19.56045 8 CATGGGA 12880 0.0 19.40595 4 >>END_MODULE