FastQCFastQC Report
Thu 26 May 2016
SRR937870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851615
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185120.9997758713339436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139500.7533963593943666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98070.5296457416903622No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42210.22796315648771479No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34320.18535170648325922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.1788168706777597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27910.15073327878635676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.139661862752246No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24730.13355908220661422No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22940.1238918457670736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200200.031.9497911
GGTATCA133000.029.3563041
TACGGTA700.0083852227.147937
TATCAAC241100.026.380352
ATCAACG243000.026.1538123
TCAACGC245150.025.9057524
CAACGCA248550.025.5322535
AACGCAG251700.025.2550166
TACATGG165150.025.1279662
GTACATG168900.024.918591
ACATGGG166900.023.7815423
GAGTACT130950.023.6339712-13
AGTACTT134300.023.0444412-13
CGCAGAG276100.022.9715148
ACGCAGA276650.022.9086727
GTACTTT140900.021.94695514-15
GCAGAGT283650.021.7894259
CATGGGG34550.021.7385564
CATGGGA129500.021.6205714
ATGGGAT48950.020.5875575
Produced by FastQC (version 0.11.5)