FastQCFastQC Report
Thu 26 May 2016
SRR937870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851615
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224761.2138592525984073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184750.9977776157570553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134620.7270409885424346No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43630.23563213735036714No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.2344979922932143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37480.2024178892480348No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36710.19825935737180786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29630.16002246687351313No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21520.11622286490442127No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.1128744366404463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.10266713112607102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164950.029.8991551
GTATCAA236150.028.5903841
TATCAAC284200.023.6871452
ATCAACG286100.023.4634173
GAGTACT164700.023.41786612-13
TCAACGC289600.023.1634434
CAACGCA292800.022.9102885
AACGCAG297400.022.5510546
TACATGG163750.022.3686642
AGTACTT167400.022.33079112-13
GTACATG168050.022.1662081
GTACTTT174250.022.1065614-15
ACATGGG165050.021.1850383
ACGCAGA328050.020.4012037
CGCAGAG328250.020.3598338
TACTTTT187700.019.86454214-15
ACTTTTT197550.019.73964116-17
GCAGAGT336100.019.5451289
CATGGGA126950.018.973274
CATGGGG35850.018.9502014
Produced by FastQC (version 0.11.5)